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Prevalence and Antibiotic Resistance Pattern of Escherichia coli and Klebsiella pneumoniae in Urine Tract Infections at the La Paz Medical Center, Malabo, Equatorial Guinea  [PDF]
Aleksey Shatalov
Open Journal of Medical Microbiology (OJMM) , 2015, DOI: 10.4236/ojmm.2015.54022
Abstract: The study was conducted to isolate and determine the antibiotic resistance in Escherichia coli and Klebsiella pneumonia from urine samples over a 2-year period (August 2013-September 2015) at the La Paz Medical Center, Malabo. A retrospective analysis of 785 urine culture samples over a 2-year period August 2013-September 2015 was carried out according to the routine protocol of urinalysis. Bacterial etiological agents were isolated from 155 (19.7%) samples with highest prevalence of Escherichia coli (55.5%) followed by Klebsiella pneumonia (23.2%), Proteus mirabilis (4.5%), Pseudomonas species (3.2%), Enterobacter species (2.6%), Enterococcus faecalis (2.6%) and others species (8.4%). The E. coli and K. pneumonia represent 78.7% of all isolated bacterial strains. The E. coli and K. pneumoniae isolates possess highly resistant to ampicillin, Trimethoprim/Sulfamethoxazole, Doxycycline, Amoxicicline/Clavulanic acid. Whereas K. pneumonia demonstrated also to be highly resistant to Gentamycin, Cefuroxime and Ceftriaxon, low level of resistance to Piperacilin/Tazobactam, Amikacin and the lowest to Imipenem. The alarming level of MDR strains to the first choice antibiotics treatment was observed.
Multi-Drug Resistance Pattern of Lactose Non-Fermenting Escherichia coli as Causative Agent of Urine Tract Infections in Luanda, Angola  [PDF]
Aleksey Shatalov
Open Journal of Medical Microbiology (OJMM) , 2019, DOI: 10.4236/ojmm.2019.91001
Abstract: This prospective study was carried out to assess the sensitivity and resistance pattern of lactose non-fermenting Escherichia coli from July 2018 to December 2018 in the Laboratory of Microbiology at Luanda Medical Center, Angola. Out of 1170 patient, a total of 120 urine specimens infected with Escherichia coli (>105 CFU/ml) were collected according to the routine protocol of urinalysis. Among these 120 isolates, 25 (21%) isolates were determined as “atypical”, lactose non-fermenting E. colis trains. The twenty-five lactose non-fermenting Escherichia coli strains isolated from urine samples in Luanda Medical Center were declared as Multiple Drugs-Resistant strains with high resistance to Cefalexine (100%), Cefuroxime (100%), Ceftriaxone (92%), Gentamycin (92%), Ciprofloxacin (72%) and Amoxiciclin/Clavulanic (80%). The alarming resistance level to the first-choice drugs for the treatment of urinary tract infections caused by non-fermentative lactose E. coli was observed.
First Case of Chromobacterium violaceum as Urinary Tract Infection Agent in Angola  [PDF]
Aleksey Shatalov, Ziv Maianski
Open Journal of Medical Microbiology (OJMM) , 2019, DOI: 10.4236/ojmm.2019.91005
Abstract: Background: Chromobacterium violaceum is a Gram-negative, a facultative anaerobe bacteria producing violacein pigment. C. violaceum is generally present as the normal flora of water and soil. The Urine Tract Infection (UTI) due to C. violaceum is very rare. Until now there was no report from Africa about UTIs caused by C. violaceum. The antimicrobial susceptibility pattern of C. violaceum is very limited due to the rarity of isolation from clinical specimens. Here, we describe the first case of urinary tract infection caused by C. violaceum in Angola. Aim: Our case report was carried out to assess the sensitivity and resistance pattern of C. violaceum as the causative agent of UTI. Results: C. violaceum was sensitive to Amoxicillin/Clavulanic acid, Ceftriaxon, Ciprofloxacin, Doxycycline, Trimethoprim/Sulfamethoxazole, Piperacilin/Tazobactam, Gentamicin, Amikacin, Aztreonam and imipenem. The bacteria showed resistance to Cefuroxime. Conclusion: Here, we report a rare case of complicate urinary tract infection caused by C. violaceum in patient, who was treated successfully with ciprofloxacin for a total duration of 7 days.
Predominance of Multi-Drug Resistant Klebsiella pneumonia and Other Gram Negative Bacteria in Neonatal Sepsis in Equatorial Guinea  [PDF]
Aleksey Shatalov, Fares Awwad, Pablo Mangue, Rami Juden Foqahaa
Open Journal of Medical Microbiology (OJMM) , 2015, DOI: 10.4236/ojmm.2015.54031
Abstract: The study was conducted on new-born babies in whom septicemia was suspected, to determine the prevalence of bacterial strains isolated and their sensitivity to antimicrobial drugs. The study was carried out at La Paz Medical Center, Microbiology section, Malabo, Equatorial Guinea from August 2013 to October 2015. Out of 293 septicemia suspected cases, 29 (10%) blood cultures were positive, 28 with bacterial growth and 1 with growth of Candida sp. The mortality rate of neonates caused by Gram negative bacterial sepsis was 34.7%. Among the Gram negative bacteria (24 isolates), the most common types were Klebsiella pneumoniae (16 = 69.6%), followed by Escherichia coli (4 = 17.4%) and Acinetobacter species (4 = 17.4%). Four Gram positive bacteria were also isolated and identified all ascoagulase-negative staphylococci. All the Klebsiella pneumoniae isolates and Acinetobacter species demonstrated Multi Drug Resistance against different antibiotics with Extended-spectrum β-lactamase (ESBL) activity. The most frequent causative agent of bacterial sepsis in new-born children was Klebsiella pneumoniae. An alarming level of Multi Drug Resistance (MDR) Klebsiella pneumoniae strains to the first choice antibiotic treatment was observed.
Low Efficiency of the Commonly Prescribed Drugs against Klebsiella pneumonia, Escherichia coli and Acinetobacter Species as the Causative Agents of Blood Stream Infection in Malabo, Equatorial Guinea  [PDF]
Aleksey Shatalov, Yaari Shilo, Rizeq Nakhash, Dennis Zhdanov
Advances in Microbiology (AiM) , 2016, DOI: 10.4236/aim.2016.63016
Abstract: The prevalence of multi drug resistant gram-negative bacteria to commonly first line drugs in blood is a serious problem in Equatorial Guinea and other world. This is the first study describing antibiotic resistance analysis of blood stream infection in Equatorial Guinea. Our study presents alarming rate of inefficiency of the most commonly prescribed drugs to treatment Klebsiella pneumoniae, Escherichia coli and Acinetobacter species isolates as the most frequency etiologic agents in blood stream infection. Out of 1849 blood culture the bacterial etiological agents were isolated from 196 (10.6%) samples. E. coli (n = 22), K. pneumonia (n = 39) and Acinetobacter (n = 17) represent 71.6% of all gram negative bacterial isolates. Almost all isolates of K. pneumonia and Acinetobacter sp. (92.1% and 100%, respectively) and about 50% of E. coli strains possessed extended-spectrum β-lactamase activity. Alarming level of multi drug resistant gram negative strains was observed. E. coli and K. pneumonia
Боулинг-клуб в г. Красноярске Боулинг-клуб в г. Красноярске
Anton Shatalov
Project Baikal , 2006,
Estimations of local thermal impact on living organisms irradiated by non-thermal microwaves
Vladimir Shatalov
Physics , 2013,
Abstract: Pennes' differential equation for bioheat transfer and the heat transfer equation are solved for the temperature distribution in a living tissue with spherical inclusions, irradiated by microwave power. It is shown that relative temperature excess in a small inclusion in the tissue in some cases is inversely proportional to its radius and does not depend on the applied power. In pulsing RF fields the effect is amplified proportionally to the ratio of the pulse period to the pulse duration. The local temperature rise significantly outpaces the averaged one and therefore the Watt to Weight SAR limits may be insufficient to estimate the safety of RF radiation and the conventional division of the biological effects of electromagnetic fields on the thermal and non-thermal needs to be revised.
School of the future: smartphones as a laboratory in pocket of each student
Vladimir Shatalov
Computer Science , 2014,
Abstract: The idea to unite student smartphones in a scalable network and use the network to collect data of personal environmental sensors and health indicators is proposed. Access to the sensors, which are available in every smartphone will provide the appropriate software. Such a monitoring at the global level would reveal the impact of the electromagnetic radiation, environmental pollution and weather factors on human health. Participation in these measurements increases the educational and social activity of students. [in Ukrainian]
On Lucas Sequences Computation  [PDF]
Aleksey Koval
Int'l J. of Communications, Network and System Sciences (IJCNS) , 2010, DOI: 10.4236/ijcns.2010.312128
Abstract: This paper introduces an improvement to a currently published algorithm to compute both Lucas "sister" sequences Vk and Uk. The proposed algorithm uses Lucas sequence properties to improve the running time by about 20% over the algorithm published in [1].
First Evidence of Surface SH-Wave Propagation in Cubic Piezomagnetics  [PDF]
Aleksey Zakharenko
Journal of Electromagnetic Analysis and Applications (JEMAA) , 2010, DOI: 10.4236/jemaa.2010.25037
Abstract: This theoretical work provides with results of characteristics calculation of the ultrasonic surface Zakharenko waves (USZWs) existing in piezomagnetic cubic monocrystals of class m3m that can be readily used for non-destructive testing. The piezomagnetic waves propagate in direction [101] corresponding to relatively easy magnetization for the following piezomagnetics: Galfenol, Terfenol-D, and CoFe2O4 with cubic structures. The phase velocities of the USZW-waves and the coefficient of magnetomechanical coupling (CMMC) K2 were calculated for the crystals. It was found that the coefficient K2 for piezomagnetics with Km2 > 1/3 and Km2 >> 1/3 is about 8% to 9%, where K2 = 2 (VUSZW,o – VUSZW,c)/VUSZW,o and Km2 = h2/(Cμ). Knowledge of piezomagnetic properties of cubic crystals makes possible the use of them in new products utilizing the phenomenon called the magnetoelectric effect. Also, this study is useful for possible application of cubic piezomagnetics in composite structures consisting of piezoelectric and (or) piezomagnetic materials and in the microwave technology. This broadens choice of possible piezomagnetic materials for utilization in various technical devices.
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