oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 44 )

2018 ( 216 )

2017 ( 232 )

2016 ( 262 )

Custom range...

Search Results: 1 - 10 of 50696 matches for " Alberto Juan; Bu Coifiu-Fanego "
All listed articles are free for downloading (OA Articles)
Page 1 /50696
Display every page Item
Respuesta neuroinmunológica en ni os con meningoencefalitis por Neisseria meningitidis.
Bárbara Padilla-Docal,Rosa Ferro-Blanco,Alberto Juan Dorta-Contreras,Raisa Bu Coifiu-Fanego
Vaccimonitor , 2011,
Abstract: La enfermedad meningocócica es una entidad clínica que constituye aún un problema de salud mundial. Esta afecta, sobre todo, a la población infantil ocasionando un cuadro clínico grave, de evolución severa, así como un elevado número de muertes y secuelas. Su agente etiológico, Neisseria meningitidis, habita de forma natural enlas membranas mucosas de la nasofaringe humana. Con el objetivo de determinar las características de la respuesta neuroinmunológica en ni os con meningoencefalitis por N. meningitidis, se estudiaron 20 ni os diagnosticados con esta afección clínica, entre los a os 1988- 2009. A estos se les realizó una punción lumbar en el momento del ingreso y se cuantificó la IgA, IgM e IgG, la albúmina y el C3c en el suero y en el líquido cefalorraquídeo.Se confeccionó un reibergrama de acuerdo con los datos obtenidos en la cuantificación. La disfunción de la barrera hematoencefálica se presentó en seis pacientes y hubo síntesis intratecal de las inmunoglobulinas mayores y C3c en 65% y 95%, respectivamente. Se comprobó la ocurrencia de síntesis intratecal de al menos dos inmunoglobulinas mayores asociadas con el componente C3c del complemento, participando estos en los mecanismos involucrados en la respuesta inmune de esta enfermedad.
Síntesis intratecal de C3c e inmunoglobulinas en ni os con meningoencefalitis bacteriana.
Bárbara Padilla-Docal,Alberto Juan Dorta-Contreras,Raisa Bu Coifiu-Fanego,Elena Noris-García
Vaccimonitor , 2008,
Abstract: Las meningoencefalitis bacterianas constituyen una fuente importante de morbilidad, mortalidad y discapacidad en diferentes regiones del mundo. El objetivo del presente trabajo es conocer si el sistema de complemento puede estar involucrado en la lisis de las bacterias productoras de meningoencefalitis a través de la liberación de C3c al líquido cefalorraquídeo. Se estudiaron siete pacientes con edad promedio de 3 a os, que ingresaron en el Hospital Pediátrico de San Miguel del Padrón, a los que se les realizó una punción lumbar diagnóstica y se les aislaron los gérmenes siguientes: Neisseria meningitidis, Streptococcus pneumoniae y Haemophilus influenzae. La cuantificación de los niveles de C3c, albúmina e inmunoglobulinas mayores en suero y líquido cefalorraquídeo se realizó en placas de inmunodifusión radial. Los resultados obtenidos fueron recogidos en un reibergrama. El total de los pacientes estudiados mostraron síntesis intratecal del componente C3c del sistema de complemento. Este hecho evidenció la activación de este sistema en alguna de sus vías y que una vez cumplidas sus funciones biológicas, ha sufrido un proceso de degradación y liberación al LCR en forma de C3c.
Síntesis intratecal de C3c e inmunoglobulinas en ni?os con meningoencefalitis bacteriana
Padilla-Docal,Bárbara; Dorta-Contreras,Alberto Juan; Bu Coifiu-Fanego,Raisa; Noris-García,Elena; Fundora-Hernández,Hermes; Callol-Barroso,Jesus; González-Hernández,Marlen;
Vaccimonitor , 2008,
Abstract: bacterial meningoencephalitis is an important source of morbidity, mortality and disabilities in different regions of the world. the objective of this paper is to know if the complement system can be involved in producing-meningoencephalitis bacterial lysis through c3c release into cerebrospinal fluid. seven patients with an average age of 3 years-old, who attended the pediatric hospital of san miguel del padrón, were studied by lumbar puncture diagnosis. neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae were isolated from the samples taken. the quantification of c3c, albumin and main immunoglobulins in serum and in cerebrospinal fluid were carried out by radial immunodifusion. results were analyzed using a reibergram. all patients showed c3c intrathecal synthesis. this fact demonstrates that the activation of this system has occurred in some of their three pathways and once its biological functions are fulfilled, it suffered a degradation and release process into cerebrospinal fluid as c3c.
Respuesta neuroinmunológica en ni?os con meningoencefalitis por Neisseria meningitidis
Padilla-Docal,Bárbara; Ferro-Blanco,Rosa; Dorta-Contreras,Alberto Juan; Bu Coifiu-Fanego,Raisa; De Paula-Almeida,Olga Susana; Martínez-Alderete,René;
Vaccimonitor , 2011,
Abstract: meningococcal disease is a clinical entity that remains as health world problem. it mostly affects children population producing gravity, a severe evolution, and a high quantity of deaths and sequels. neisseria meningitidis is the etiological agent. the mucosa membranes of human nasopharynx are its natural habitat. this paper aims at establishing and determining the characteristics of the neuroimmunological response in children suffering from meningoencephalitis caused by neiseria meningitidis. twenty children with this diagnosis from 1998 and 2009 were studied. cerebrospinal fluid lumbar puncture and blood extraction were performed. samples were collected at the time of admission of the children to the hospital. iga, igg, igm, c3c and albumin were quantified. a reibergram was done according to the data obtained in the quantification. blood-cerebrospinal fluid barrier dysfunction was present in 6 of studied patients and there was intrathecal synthesis of major immunoglobulins and c3c in 65 and 95% respectively. the occurrence of intrathecal synthesis of at least 2 types of major immunoglobulins associated to c3c was demonstrated, which participate in the mechanisms involved in the immune response in this disease.
sICAM-1 intrathecal synthesis and release during the acute phase in children suffering from Coxsackie A9 and S. pneumoniae meningoencephalitis
Dorta-Contreras, Alberto J.;Lewczuk, Piotr;Padilla-Docal, Bárbara;Noris-García, Elena;Coifiu-Fanego, Raisa Bu;Sánchez-Martínez, Consuelo;Rodríguez-Rey, Alexis;González-Hernández, Marlén;
Arquivos de Neuro-Psiquiatria , 2008, DOI: 10.1590/S0004-282X2008000400013
Abstract: the intercellular adhesion molecule is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. serum and cerebrospinal fluid (csf) soluble intercellular adhesion molecule 1 (sicam-1) from normal control children as well as from children with guillain-barré syndrome (gbs), with coxsackie a9 virus meningoencephalitis and with streptococcus pneumoniae meningoencephalitis were studied. sicam-1 was quantified using an immunoenzimatic assay and albumin using the immunodiffusion technique in both biological fluids. increased sicam-1 values in csf in patients with gbs correspond to an increase of the albumin csf/serum quotient. in contrast, in inflammatory diseases like s. pneumoniae and coxsackie a9 virus meningoencephalitis an increased brain-derived fraction was observed. in particular cases these values are 60-65% and 70-75% respectively. the results indicate an additional synthesis of sicam-1 in subarachnoidal space during central nervous system (cns) inflammatory process. an important role of sicam-1 in the transmigration of different cell types into csf during cns inflammation in children with s. pneumoniae and coxsackie a9 meningoencephalitis may be suggested.
Using reibergrams to evaluate the intrathecal synthesis of C3c and C4 in children affected with Neisseria meningitidis meningoencephalitis
Dorta-Contreras,Alberto J; Padilla-Docal,Bárbara; Coifiu-Fanego,Raisa B;
Biotecnolog?-a Aplicada , 2011,
Abstract: meningococcal meningoencephalitis was a serious pediatric health problem in cuba before the successful introduction of a cuban vaccine against neisseria meningitidis. the present paper assesses the role of the complement system in this disease in unvaccinated sick children, using a novel methodology developed by the authors. seven children were used, of an average of 5.8 years of age with n. meningitidis meningoencephalitis diagnosed by traditional microbiological methods. serum and cerebrospinal fluid samples were obtained at the same time point, and used to quantify major immunoglobulins, igg subclasses, c3c, c4 and albumin by radial immunodiffusion with commercially available plates. no c3c was found in one of the two deceased patients. measurable intrathecal synthesis of c3c was observed in the remaining patients, however, intrathecal synthesis of c4 was found in all patients, as demonstrated with the corresponding reibergrams. the measurement of the intrathecal synthesis of these components of the complement system is useful for discriminating immunodeficiencies and to better understand the behavior of the disease.
Neuroimmunological findings of Angiostrongylus cantonensis meningitis in ecuadorian patients
Dorta-Contreras, Alberto J.;Padilla-Docal, Barbara;Moreira, Juan M.;Robles, Luiggi Martini;Aroca, Jenny Muzzio;Alarcón, Fernando;Bu-Coifiu- Fanego, Raisa;
Arquivos de Neuro-Psiquiatria , 2011, DOI: 10.1590/S0004-282X2011000400011
Abstract: meningitis caused by angiostrongylus cantonensis has recently been reported in patients resulting from the first outbreaks in subtropical regions of ecuador. method: eight young adult patients from the two outbreaks were studied. iga, igm, igg and albumin in cerebrospinal fluid and serum were quantified and plotted in cerebrospinal fluid/serum quotient diagrams (reibergrams). the anamnesis on the patients included asking about any consumption of raw snails, symptoms and harm caused. results: mean eosinophilia of 7.5% and 26% in serum and cerebrospinal fluid respectively was observed, as well as a moderate increase in total proteins. the most frequent pattern of intrathecal synthesis was observed in three classes of immunoglobulins. intrathecal synthesis of igm was observed in all cases two weeks after the first symptoms appeared. conclusion: the intrathecal synthesis patterns of eosinophilic meningitis due to angiostrongylus cantonensis, facilitated by cerebrospinal fluid analysis, were similar to those of previous cases from abroad.
Reibergrama para la evaluación de la síntesis intratecal de C3c
Dorta-Contreras, Alberto Juan;Noris-García, Elena;Padilla-Docal, Bárbara;Rodríguez-Rey, Alexis;Bu-Coifiu-Fanego, Raisa;Magraner-Tarrau, María Esther;Martí-Brenes, Mauricio;
Arquivos de Neuro-Psiquiatria , 2006, DOI: 10.1590/S0004-282X2006000400010
Abstract: introduction: reiber's quotient diagram or reibergram has a growing apply for characterize the intratecal synthesis of proteins. firstly reibergrama was used for the major classes of immunoglobulins but later it was used to evaluate other proteins based on the theory about molecular flux/cerebrospinal fluid (csf) flow rate. method: c3c is a degradation product of complement factor c3 with 145 kda and approaches to igg molecular characteristics according with fick's diffussion laws. it was assumed igg constants and graphic for igg constants and graphic to evaluate the intrathecal synthesis of c3c. twenty-seven patients and 27 controls were studied. serum and csf c3c and albumin were quantified by immunodiffusion. results: the patients with the c3c proposed reibergram were evaluated. it has been proved its validity under several csf blood barrier conditions. conclusion: reibergram for c3c can be used for the evaluation of the intrathecal synthesis of this protein.
Enolasa específica de neurona en dos recién nacidos con depresión ligera al nacer Neuron- specific Enolase in two newborns presenting with moderate depression at birth
Raisa Bu-Coifiu Fanego,Alberto Dorta Contreras,Elena Noris García,Barbara Padilla Docal
Revista Cubana de Pediatr?-a , 2009,
Abstract: INTRODUCCIóN. La enolasa específica de neurona es una isoenzima que se vierte al torrente sanguíneo después de un episodio de da o neuronal. En los procesos de hipoxia neonatal estos valores enzimáticos en suero suelen estar alterados. El objetivo del presente artículo fue estudiar la enolasa específica de neurona en suero de 2 recién nacidos con Apgar bajo y determinar si, en el seguimiento durante un a o, dichos pacientes presentaban trastornos del desarrollo psicomotor. MéTODOS. Se tomaron muestras de suero al momento del nacimiento y a las 72 h siguientes. Se determinaron los niveles de enolasa específica de neurona por un método inmunoenzimático de tipo ELISA. Cada muestra fue evaluada por el método de reacción en cadena de la polimerasa para citomegalovirus, en el Instituto de Inmunología de Wuersburg (Alemania). También se cuantificaron anticuerpos contra citomegalovirus de clase IgM e IgG, en el Laboratorio de Neuroquímica de la Universidad Georg August de Goettingen (Alemania). Se recogieron los datos clínicos de interés de cada recién nacido y al a o se citaron a estos pacientes y se les realizó un examen físico para evaluar su neurodesarrollo. RESULTADOS. Las cifras de enolasa estuvieron incrementadas tanto al nacimiento como a las 72 h, con anticuerpos anticitomegalovirus de clase IgG que fueron transferidos de la madre a través de la placenta. No se encontró presencia de este virus en el momento del nacimiento. En el examen físico y neurológico realizado al a o se constató que los ni os evolucionaban satisfactoriamente hasta esa fecha. CONCLUSIONES. Se recomienda extender el estudio hasta los 3 a os de vida y aumentar el número de pacientes estudiados, con énfasis en aquellos casos cuyo Apgar es menor de 5 a los 5 min del nacimiento. INTRODUCTION: Neuron-specific Enolase of is an isoenzyme present in blood stream after a neuronal damage episode. In processes of neonatal hypoxia, these enzymatic values in serum may be altered. The aim of present paper was to study Enolase specific of neuron in the serum of two newborns with low Apgar score and to determine if, during a one year follow-up, such patients presented disorders of psychomotor development. METHODS: We took serum samples at birth and at 72 hours. Levels of Enolase specific of neuron by immunoenzymatic method type ELISA were determined. Each sample was assessed by polymerase chain reaction (PCR) to cytomegalovirus in Wuersburg Institute of Immunology (Germany). Also, we quantified antibodies to IgM and IgG cytomegalovirus in Neurochemistry Institute of Georg August of Goettingen Univ
CSF/serum quotient graphs for the evaluation of intrathecal C4 synthesis
Barbara Padilla-Docal, Alberto J Dorta-Contreras, Raisa Bu-Coifiu-Fanego, Alexis Rey
Fluids and Barriers of the CNS , 2009, DOI: 10.1186/1743-8454-6-8
Abstract: The constants in the previously-defined Reibergram for immunoglobulin IgA were used to calculate the CSF/serum quotient for C4. CSF and serum were analyzed for C4, IgA and albumin from a total of 12 patients with meningoencephalitis caused by encapsulated microorganisms and 10 subjects without infections or inflammatory neurological disease, some of which had dysfunction of the blood-CSF barrier,The formula and C4 Reibergram with the constants previously found for IgA, determined the intrathecal C4 synthesis in CSF. The intrathecal C4 fraction in CSF (C4 loc in mg/l) was compared to the C4-Index (fraction of CSF: serum for C 4/fraction of CSF: serum for albumin). There was a significant correlation between the two formulae. The CSF/Serum quotient graph was superior for detecting intrathecal synthesis of C4 under variable conditions of blood-CSF barrier permeability.The C4 Reibergram can be used to quantify the intrathecal synthesis of this component of the complement system in different infectious diseases of the central nervous system and is especially useful for patients with blood-brain barrier dysfunction.Cerebrospinal fluid (CSF) analysis has great potential for the diagnosis of neurological diseases. Reibergrams or CSF/serum quotient graphs [1,2] are diagrams that analyze in an integrated way both the function of the blood-CSF barrier and intrathecal protein synthesis, to aid in the diagnosis of central nervous system (CNS) diseases associated with specific patterns of immunoglobulin response. This diagram was first defined for the major classes of immunoglobulins [2,3] empirically based on the results of thousands of profiles and was subsequently confirmed by application of Fick's law of diffusion in the theory of molecular diffusion/flow rate [4]. Fick's law states that the diffusion of a protein through a barrier depends on the diffusion coefficient of the molecule and on the concentration gradient between the compartments on either side of the barrier. The
Page 1 /50696
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.