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Search Results: 1 - 10 of 314743 matches for " Alberto J. Prioli "
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Identification of Astyanax altiparanae (Teleostei, Characidae) in the Igua u River, Brazil, based on mitochondrial DNA and RAPD markers
Prioli S?nia M.A.P.,Prioli Alberto J.,Júlio Jr. Horácio F.,Pavanelli Carla S.
Genetics and Molecular Biology , 2002,
Abstract: Astyanax fishes are among the most important food-web components of South America rivers. In the Igua u River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Igua u Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti & Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Igua u River, upstream from the Igua u Falls. Mitochondrial DNA sequences and RAPD markers revealed high genetic diversity within each population, as well as low genetic distance, high gene flow, and high mitochondrial DNA similarity among all three populations. In conjunction with morphological similarities, these results demonstrated that the population presently known as Astyanax bimaculatus in the Igua u River should actually be stated as Astyanax altiparanae. Furthermore, it could be inferred that the A. altiparanae population is not endemic and most likely it was recently introduced in the Igua u River basin, maintaining the ancestral genetic identity.
Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini)
Juliana Bernardi-Wenzel,Adriana García,Celso J R Filho,Alberto J Prioli
Biological Research , 2010,
Abstract: Endophyte microorganisms are organisms that live inside plants without causing any apparent damage to their hosts. Since all plants exhibit endophyte microorganisms, it is believed that mutual association is of great importance in nature. Luehea divaricata (Martius & Zuccarini), known popularly in Brazil as agoita-cavalo, is a big-sized tree with a wide distribution in the country that possesses medicinal qualities for: dysentery, leucorrhea, rheumatism, blennorrhoea, tumors, bronchitis, and depuration. This research aims at isolating and molecularly characterizing fungi isolates from L. divaricata by sequence analysis of ITS1-5.8S-ITS2 rDNA. Further, the colonization of endophyte in the host plant by Light and Scanning Electron Microscopy will also be investigated. Whereas, genera Alternaria, Cochliobolus, Diaporthe, Epicoccum, Guignardia, Phoma, and Phomopsis, were identified; rDNA sequence analysis revealed intra-species variability among endophyte isolates of the genus Phomopsis sp. Light and Scanning Electron Microscopy techniques showed the presence of endophyte fungi inside L. divaricata leaves, inhabiting inter- and intra-cellular spaces. These types of extensive colonization and dissemination were reported throughout all the leaf parts in palisade parenchyma, esclerenchyma, spongy parenchyma, adaxial epidermis, and vascular bundle indicating colonization of endophytes in múltiple structural sub-niches in the host plant.
Identification of Astyanax altiparanae (Teleostei, Characidae) in the Igua?u River, Brazil, based on mitochondrial DNA and RAPD markers
Prioli, S?nia M.A.P.;Prioli, Alberto J.;Júlio Jr., Horácio F.;Pavanelli, Carla S.;Oliveira, Alessandra V. de;Carrer, Helaine;Carraro, Dirce M.;Prioli, Laudenir M.;
Genetics and Molecular Biology , 2002, DOI: 10.1590/S1415-47572002000400011
Abstract: astyanax fishes are among the most important food-web components of south america rivers. in the igua?u river basin, the astyanax genus is represented mainly by endemic species. for millions of years, that hydrographic basin has been geographically isolated from the paraná river basin by the igua?u falls. recently, a species from the upper paraná river basin identified as astyanax bimaculatus was revised and described as a new species named astyanax altiparanae garutti & britski, 2000. fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two a. altiparanae populations from the upper paraná river basin and the population identified as a. bimaculatus in the igua?u river, upstream from the igua?u falls. mitochondrial dna sequences and rapd markers revealed high genetic diversity within each population, as well as low genetic distance, high gene flow, and high mitochondrial dna similarity among all three populations. in conjunction with morphological similarities, these results demonstrated that the population presently known as astyanax bimaculatus in the igua?u river should actually be stated as astyanax altiparanae. furthermore, it could be inferred that the a. altiparanae population is not endemic and most likely it was recently introduced in the igua?u river basin, maintaining the ancestral genetic identity.
Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini)
Bernardi-Wenzel,Juliana; García,Adriana; Filho,Celso J R; Prioli,Alberto J; Pamphile,Jo?o A;
Biological Research , 2010, DOI: 10.4067/S0716-97602010000400001
Abstract: endophyte microorganisms are organisms that live inside plants without causing any apparent damage to their hosts. since all plants exhibit endophyte microorganisms, it is believed that mutual association is of great importance in nature. luehea divaricata (martius & zuccarini), known popularly in brazil as agoita-cavalo, is a big-sized tree with a wide distribution in the country that possesses medicinal qualities for: dysentery, leucorrhea, rheumatism, blennorrhoea, tumors, bronchitis, and depuration. this research aims at isolating and molecularly characterizing fungi isolates from l. divaricata by sequence analysis of its1-5.8s-its2 rdna. further, the colonization of endophyte in the host plant by light and scanning electron microscopy will also be investigated. whereas, genera alternaria, cochliobolus, diaporthe, epicoccum, guignardia, phoma, and phomopsis, were identified; rdna sequence analysis revealed intra-species variability among endophyte isolates of the genus phomopsis sp. light and scanning electron microscopy techniques showed the presence of endophyte fungi inside l. divaricata leaves, inhabiting inter- and intra-cellular spaces. these types of extensive colonization and dissemination were reported throughout all the leaf parts in palisade parenchyma, esclerenchyma, spongy parenchyma, adaxial epidermis, and vascular bundle indicating colonization of endophytes in múltiple structural sub-niches in the host plant.
Embriogênese somática a partir de embri?es imaturos em genótipos de milho
Fernandes, Eloise Helena;Prioli, Alberto José;Scapim, Carlos Alberto;Schuster, Ivan;Vieira, Elisa Serra Negra;Amaral Júnior, Ant?nio Teixeira do;Moterle, Lia Mara;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000900031
Abstract: the establishment of a protocol for regenerating plants by tissue culture is the first step in breeding programs which have the objective of using genetic transformation on plants. the plant regeneration can be achieved either by organogenesis or embryogenesis. in the second case, the somatic embryogenesis depends on the identification of responsive genotypes which enhance the efficiency of the program. the aim of the present study was to identify maize genotypes with high capacity to produce somatic embryos and consequently regenerate maize plants. eleven genotypes (inbreds and hybrids) were investigated in the present experiment. under two-week periods, the cultures were obtained from immature embryos which were inoculated into growing medium n6 with 690mg l-1 of proline and 10mm of 2,4-d. callis of type ii, friable and embryogenic, were observed in the ld82025, cd308, and cml314 genotypes. after, they were submitted to the regeneration process and the best performance was achieved by the ld82025. no embryogenic callus was developed from cd307, cd304, oc-705, 105-b, and gu04328. in the present case, the inbred ld82025 is the most promising maize genotype for participating in a breeding program that will use the genetic transformation of maize plants.
Inheritance of aluminum tolerance in maize
Talge Aiex Boni,Alberto José Prioli,S?nia Maria Alves Pinto Prioli,Léia Carolina Lucio
Crop Breeding and Applied Biotechnology , 2009,
Abstract: The purpose of this study was to investigate aluminum tolerance in maize. For this purpose, a nutrient solutionwas used with the tolerant (L922) and sensitive (Ast214) homozygous parental lines, with the segregating generation F2 andwith the F2:3 lines derived thereof. Seeds were germinated on paper and seedlings transferred to a nutrient solution containing4.5 mg L-1 aluminum. In the experiments involving F2:3 derived lines groups of rows with F2:3 plants were intercropped withrows of eight seedlings of the parental lines in a completely randomized block design. Results of the F2 generation demonstrateda bimodal distribution of relative frequencies, with approximately three times more seedlings in the tolerant group. Thissuggests the participation of only one locus in tolerance inheritance. The narrow-sense heritability (F2, F2:3) of seminal rootlength of F2 plants and means of F2:3 lines was 0.49, which partially explained the bimodal frequency distribution of means ofF2:3 lines, but was not accurate enough to differentiate tolerant from sensitive groups.
Genetic divergence between Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Siluriformes: Pimelodidae) in the Paraná River Basin
Bignotto, TS.;Prioli, AJ.;Prioli, SMAP.;Maniglia, TC.;Boni, TA.;Lucio, LC.;Gomes, VN.;Prioli, RA.;Oliveira, AV.;Júlio-Junior, HF.;Prioli, LM.;
Brazilian Journal of Biology , 2009, DOI: 10.1590/S1519-69842009000300022
Abstract: pseudoplatystoma corruscans (spix and agassiz, 1829) and pseudoplatystoma reticulatum (eingenmann and eigenmann, 1889) are large migratory catfishes of high biological importance and great commercial value in south america. because fertile crossbreeds can be artificially produced in hatcheries, a high genetic proximity between these two pimelodidae species is conceivable. possible escape of crossbred specimens from pisciculture stations is a serious environmental concern. despite their importance, knowledge of p. corruscans and p. reticulatum biology, ecology, population diversity and genetics is limited. in the present work, the genetic divergence between p. corruscans and p. reticulatum populations from the paraná river basin was analyzed on the basis of polymorphisms in issr fragments and in the hypervariable sequence of the mitochondrial dna (mtdna) control region. estimates of intraspecific haplotype (h > 0.5) and nucleotide diversities (π < 0.01) indicate that p. corruscans and p. reticulatum have survived a historical population decline, followed by a demographic expansion. the interspecific polymorphisms within the mtdna control region and issr fragments were suitable as diagnostic molecular markers and could be used to discriminate the two species. a unique pseudoplatystoma specimen, captured in the upper paraná river floodplain, was identified by these dna diagnostic markers as a hybrid p. reticulatum x p. corruscans, which possibly escaped from pisciculture. the integrity of the natural population of p. corruscans in the upper paraná river is at risk of genetic introgression or homogenization due to the presence of hybrids and the transposition of p. reticulatum upstream through the canal da piracema at itaipu dam. data presented herein improve the understanding of the genetic relatedness between p. corruscans and p. reticulatum and represent potential tools for future programs of conservation and surveillance of genetic introgression events and the genet
Genetic variability of brycon orbignyanus (valenciennes, 1850) (characiformes: characidae) in cultivated and natural populations of the upper paraná river, and implications for the conservation of the species
Panarari-Antunes, Renata de Souza;Prioli, Alberto José;Prioli, S?nia Maria Alves Pinto;Galdino, Alexsandro Sobreira;Julio Junior, Horácio Ferreira;Prioli, Laudenir Maria;
Brazilian Archives of Biology and Technology , 2011, DOI: 10.1590/S1516-89132011000400025
Abstract: this study aimed to evaluate the genetic variability of b. orbignyanus in cultivated and natural populations of the upper paraná river, using molecular rapd markers and mtdna control region. specimens were collected in the paraná river and in the piracema fish farm in maringá, state of paraná, brazil. rapd primers produced 82 loci with consistent expression. the population from the paraná river showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. data revealed genetic differentiation between the two populations, although not very pronounced. these results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. the alignment of the d-loop sequences of b. orbignyanus indicated the existence of polymorphism only in the natural population. these data could be helpful for the formulation of management strategies and conservation of the genetic diversity of the species.
Obtaining 5S rDNA molecular markers for native and invasive Cichla populations (Perciformes – Cichlidae), in Brazil = Obten o de marcadores moleculares 5S rDNA para popula es nativas e introduzidas de Cichla (Perciformes – Cichlidae), do Brasil
Viviane Fátima de Oliveira,Alessandra Valéria de Oliveira,Alberto José Prioli,S?nia Maria Alves Pinto Prioli
Acta Scientiarum : Biological Sciences , 2008,
Abstract: The 5S rDNA gene is informative and has high conservation rates along the eukaryotic genome, having unique hereditary characteristics. Molecular studies with the 5S rDNA gene have been carried out with several groups, including some species of fish, aiming at solving phylogenetic relationship problems, ancestral patterns and geneticdiversity among groups in natural populations. Species of the Cichla genus, introduced in the upper Paraná river basin, present some genetic polymorphisms detected by RAPD and SPAR analyses. These species have been intercrossing and forming viable hybrids, withgreater genetic variability. The objective of this work was to standardize the amplification methodology for the non-transcribed regions of 5S rDNA multigenic family of Cichla, and to obtain specific markers for parent species that could also be identified in the hybrids. Sixty-five specimens of Cichla collected from the upper Paraná river and Amazon basins were analyzed. Although molecular markers that could be useful in the identification of hybrids were not obtained, genetic molecular 5S rDNA species-specific markers for Cichla temensis that can be employed to identify of this species, as well population markers that can be useful in population genetic variability studies, were obtained. O gene DNAr 5S é informativo e possui altas taxas de conserva o ao longo do genoma dos eucariotos, possuindo características únicas que s o hereditárias. Estudos moleculares do gene DNAr 5S vem sendo realizados com diversos grupos, inclusive em algumas espécies de peixes, com o intuito de solucionar problemas de rela es filogenéticas, padr o de ancestralidade e diversidade genética, entre grupos de popula es naturais. Espécies do gênero Cichla, introduzidas na bacia do alto rio Paraná, apresentam polimorfismos genéticos, detectados por análise de RAPD e SPAR. Essas espécies est o intercruzando-se e formando híbridos viáveis, com maior variabilidade genética. O objetivo desse trabalho foi padronizar ametodologia de amplifica o de regi es n o-transcritas da família multigênica rDNA 5S de Cichla e obter marcadores específicos para as espécies parentais que pudessem, também, ser identificados nos híbridos. Foram analisados 65 espécimes de Cichla, das bacias do alto rio Paraná e Amaz nica. Apesar de n o se obter marcadores moleculares que pudessem ser úteis à identifica o de híbridos, foram obtidos marcadores moleculares genéticos DNAr 5S espécie-específicos para Cichla temensis, que podem ser utilizados para identifica o deexemplares dessa espécie e, também, marcadores populacionai
Spar genetic analysis of two invasive species of Cichla (Tucunaré) (Perciformes: Cichlidae) in the Paraná river basin = Análise genética via spar, de duas espécies de Cichla (Tucunaré) (Perciformes: Cichlidae) introduzidas na Bacia do rio Paraná
Giovanna Caputo Almeida-Ferreira,Alessandra Valéria de Oliveira,Alberto José Prioli,S?nia Maria Alves Pinto Prioli
Acta Scientiarum : Biological Sciences , 2011,
Abstract: The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be foundin other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti) belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current research used SPAR molecular markers to confirm the presence of hybrids and the rupture of isolation mechanisms. Seventy-two specimens collected in several sites of the river Paraná and Amazon basins were analyzed. Since exclusive SPAR molecular markers were obtained for Cichla kelberi and C. piquiti populations, the introduction of the two species in the region has been confirmed. Identification of the markers in specimens of theParaná river basin confirmed hybridization between these exotic species. A introdu o de espécies exóticas nos ecossistemas lacustres tem sido muito destacada na literatura mundial, pois amea a a diversidade de peixes nativos, tornando-se uma quest o de extrema importancia. Os efeitos observados podem ser ecológicos, como preda o, competi o e parasitismo ou genéticos, como a ocorrência de híbridos. Apesar do peixe tucunaré (Cichla) ser nativo da regi o amaz nica, ele pode ser encontrado em outras bacias hidrográficas nas quais foram introduzidos. Estudos utilizando marcadores moleculares RAPD revelaram que existem duas espécies (Cichla kelberi e C. piquiti) do gênero Cichla na bacia do alto rio Paraná e morfotipos diferentes que podem ser devido à hibridiza o. O presente trabalho utilizou marcadores moleculares SPAR para confirmar a presen a de híbridos e os mecanismos de ruptura de isolamento entre essas espécies. Setenta e dois espécimes foram coletados em diversos pontos da bacia do rio Paraná e da bacia Amaz nica e foram analisados. Marcadores moleculares SPAR exclusivos foram obtidos para popula es de Cichla kelberi e C. piquiti, confirmando a introdu o das duas espécies na regi o. A identifica o dos marcadores diagnósticos em espécimes da bacia do rio Paraná confirmou também a hibridiza o entre estas espécies exóticas.
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