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Search Results: 1 - 10 of 26427 matches for " Ah-Young Kim "
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Comparison of Movement of the Upper Dentition According to Anchorage Method: Orthodontic Mini-Implant versus Conventional Anchorage Reinforcement in Class I Malocclusion
Ah-Young Lee,Young Ho Kim
ISRN Dentistry , 2011, DOI: 10.5402/2011/321206
The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis
Kathryn Kamo, Ah-Young Kim, Se Park, Young Joung
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-79
Abstract: Gladiolus and Arabidopsis were verified by Southern hybridization to be transformed with the uidA gene that was under control of either the GUBQ1 promoter (1.9?kb), a 5′ GUBQ1 promoter missing its 1.234?kb intron (0.68?kb), or the CaMV 35?S promoter. Histochemical staining showed that GUS was expressed throughout leaves and roots of Gladiolus and Arabidopsis with the 1.9?kb GUBQ1 promoter. GUS expression was significantly decreased in Gladiolus and abolished in Arabidopsis when the 5′UTR-intron was absent. In Arabidopsis and Gladiolus, the presence of uidA mRNA was independent of the presence of the 5′UTR-intron. The 5′-UTR intron enhanced translation efficiency for both Gladiolus and Arabidopsis.The GUBQ1 promoter directs high levels of GUS expression in young leaves of both Gladiolus and Arabidopsis. The 5′UTR-intron from GUBQ1 resulted in a similar pattern of β-glucuronidase translation efficiency for both species even though the intron resulted in different patterns of uidA mRNA accumulation for each species.There are many non-cereal monocots of agronomic (garlic, onions, banana, sugarcane, millet), horticultural (turf and forage grasses such as tall fescue, Kentucky bluegrass, creeping bentgrass, perennial ryegrass, St. Augustinegrass, buffalograss, Zoysiagrass japonica, Paspalum vaginatum, Paspalum notatum, floral crops such as orchids, lilies, tulips, crocus, amaryllis, iris), and potential biofuel (switchgrass, Miscanthus) importance. Successful transformation of these species requires an understanding of how their gene expression is controlled by promoter elements such as introns.Introns have been found to significantly stimulate gene expression. Dicot introns have been used to transform dicot plants, and levels of intron-mediated expression (IME) in leaves ranged from 2–20× [1-4]. Monocot introns have been shown to enhance gene expression in monocots 20–100× [5-10]. Most of the studies with monocots have been transient transformation studies using callus c
DNA Methyltransferase Controls Stem Cell Aging by Regulating BMI1 and EZH2 through MicroRNAs
Ah-Young So,Ji-Won Jung,Seunghee Lee,Hyung-Sik Kim,Kyung-Sun Kang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019503
Abstract: Epigenetic regulation of gene expression is well known mechanism that regulates cellular senescence of cancer cells. Here we show that inhibition of DNA methyltransferases (DNMTs) with 5-azacytidine (5-AzaC) or with specific small interfering RNA (siRNA) against DNMT1 and 3b induced the cellular senescence of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) and increased p16INK4A and p21CIP1/WAF1 expression. DNMT inhibition changed histone marks into the active forms and decreased the methylation of CpG islands in the p16INK4A and p21CIP1/WAF1 promoter regions. Enrichment of EZH2, the key factor that methylates histone H3 lysine 9 and 27 residues, was decreased on the p16INK4A and p21CIP1/WAF1 promoter regions. We found that DNMT inhibition decreased expression levels of Polycomb-group (PcG) proteins and increased expression of microRNAs (miRNAs), which target PcG proteins. Decreased CpG island methylation and increased levels of active histone marks at genomic regions encoding miRNAs were observed after 5-AzaC treatment. Taken together, DNMTs have a critical role in regulating the cellular senescence of hUCB-MSCs through controlling not only the DNA methylation status but also active/inactive histone marks at genomic regions of PcG-targeting miRNAs and p16INK4A and p21CIP1/WAF1 promoter regions.
Lactobacillus brevis Strains from Fermented Aloe vera Survive Gastroduodenal Environment and Suppress Common Food Borne Enteropathogens
Young-Wook Kim, Young-Ju Jeong, Ah-Young Kim, Hyun-Hee Son, Jong-Am Lee, Cheong-Hwan Jung, Chae-Hyun Kim, Jaeman Kim
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090866
Abstract: Five novel Lactobacillus brevis strains were isolated from naturally fermented Aloe vera leaf flesh. Each strain was identified by Random Amplified Polymorphic DNA (RAPD) analysis and 16S rRNA sequence comparison. These strains were highly tolerant to acid, surviving in pH2.5 for up to 4 hours, and resistant to 5% bile salts at 37°C for 18 hours. Due to its tolerance to acid and bile salts, one strain passed through the gastric barrier and colonised the intestine after oral administration. All five strains inhibited the growth of many harmful enteropathogens without restraining most of normal commensals in the gut and hence named POAL (Probiotics Originating from Aloe Leaf) strains. Additionally, each strain exhibited discriminative resistance to a wide range of antibiotics. The L. brevis POAL strains, moreover, expressed high levels of the glutamate decarboxylase (GAD) gene which produces a beneficial neurotransmitter, γ-aminobutyric acid (GABA). These characteristics in all suggest that the novel L. brevis strains should be considered as potential food additives and resources for pharmaceutical research.
Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice
Moon-Jung Goo,Jin-Kyu Park,Il-Hwa Hong,Ah-Young Kim,Eun-Mi Lee,Eun-Joo Lee,Meeyul Hwang,Kyu-Shik Jeong
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140611084
Abstract: Recently, senescence marker protein-30 (SMP30) knockout (KO) mice have been reported to be susceptible to apoptosis, however, the role of SMP30 has not been characterized in the small intestine. The aim of the present study is to investigate the role of SMP30 in the process of spontaneous and γ-radiation-induced apoptosis in mouse small intestine. Eight-week-old male wild-type (WT) mice and SMP30 KO mice were examined after exposure to 0, 1, 3, 5, and 9 Gy of γ-radiation. Apoptosis in the crypts of the small intestine increased in the 0 to 5 Gy radiated SMP30 KO and WT mice. Radiation-induced apoptosis and the BAX/Bcl-2 ratio in the SMP30 KO mice were significantly increased in comparison to each identically treated group of WT mice ( p < 0.05). The levels of spontaneous apoptosis in both WT and KO mice were similar ( p > 0.05), indicating that increased apoptosis of crypt cells of SMP30 KO by irradiation can be associated with SMP30 depletion. These results suggested that SMP30 might be involved in overriding the apoptotic homeostatic mechanism in response to DNA damage.
Smad3 Deficiency Ameliorates Hepatic Fibrogenesis through the Expression of Senescence Marker Protein-30, an Antioxidant-Related Protein
Da-Hee Jeong,Meeyul Hwang,Jin-Kyu Park,Moon-Jung Goo,Il-Hwa Hong,Mi-Ran Ki,Akihito Ishigami,Ah-Young Kim,Eun-Mi Lee,Eun-Joo Lee,Kyu-Shik Jeong
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141223700
Abstract: Smad3 is a key mediator of the transforming growth factor (TGF)-β1 signaling pathway that plays central role in inflammation and fibrosis. In present study, we evaluated the effect of Smad3 deficiency in Smad3?/? mice with carbon tetrachloride (CCl 4)-induced liver fibrosis. The animals were received CCl 4 or olive oil three times a week for 4 weeks. Histopathological analyses were performed to evaluate the fibrosis development in the mice. Alteration of protein expression controlled by Smad3 was examined using a proteomic analysis. CCl 4-induced liver fibrosis was rarely detected in Smad3?/? mice compared to Smad3+/+. Proteomic analysis revealed that proteins related to antioxidant activities such as senescence marker protein-30 ( SMP30), selenium-binding proteins (SP56) and glutathione S-transferases (GSTs) were up-regulated in Smad3?/? mice. Western blot analysis confirmed that SMP30 protein expression was increased in Smad3?/? mice. And SMP30 levels were decreased in CCl 4-treated Smad3 + / + and Smad3?/? mice. These results indicate that Smad3 deficiency influences the proteins level related to antioxidant activities during early liver fibrosis. Thus, we suggest that Smad3 deteriorate hepatic injury by inhibitor of antioxidant proteins as well as mediator of TGF-β1 signaling.
Knockdown of the Sodium-Dependent Phosphate Co-Transporter 2b (NPT2b) Suppresses Lung Tumorigenesis
Seong-Ho Hong, Arash Minai-Tehrani, Seung-Hee Chang, Hu-Lin Jiang, Somin Lee, Ah-Young Lee, Hwi Won Seo, Chanhee Chae, George R. Beck, Myung-Haing Cho
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0077121
Abstract: The sodium-dependent phosphate co-transporter 2b (NPT2b) plays an important role in maintaining phosphate homeostasis. In previous studies, we have shown that high dietary inorganic phosphate (Pi) consumption in mice stimulated lung tumorigenesis and increased NPT2b expression. NPT2b has also been found to be highly expressed in human lung cancer tissues. The association of high expression of NPT2b in the lung with poor prognosis in oncogenic lung diseases prompted us to test whether knockdown of NPT2b may regulate lung cancer growth. To address this issue, aerosols that contained small interfering RNA (siRNA) directed against NPT2b (siNPT2b) were delivered into the lungs of K-rasLA1 mice, which constitute a murine model reflecting human lung cancer. Our results clearly showed that repeated aerosol delivery of siNPT2b successfully suppressed lung cancer growth and decreased cancer cell proliferation and angiogenesis, while facilitating apoptosis. These results strongly suggest that NPT2b plays a role lung tumorigenesis and represents a novel target for lung cancer therapy.
Investigations on Structural Safety of Office Room Based on Fire Simulation and Transient Heat Transfer Analysis  [PDF]
Ah Young An, Eun Mi Ryu, Hee Sun Kim
World Journal of Engineering and Technology (WJET) , 2014, DOI: 10.4236/wjet.2014.23B004

This study aims at investigating heat propagations inside the structural members due to fire using fire simulation and transient heat transfer analysis. Toward that goal, fire simulation and transient heat transfer analysis for a 5-story building are carried out sequentially using Fire Dynamics Simulator (FDS) and ABAQUS 6.10-3, respectively. As results from fire simulation, temporal temperature information is obtained depending on various locations of the building, which is used as boundary condition for the structural elements generated in transient heat transfer analysis. Predictions from the transient heat transfer analysis show that the structural members are exposed spatially non-uniform temperatures which can cause significant eccentric deformation and aceleration of structural damages.

Gingival peripheral odontoma in a child: Case report of an uncommon lesion  [PDF]
Ji Hyun Kwon, Young Ah Cho, Hong Keun Hyun, Teo Jeon Shin, Young Jae Kim
Open Journal of Stomatology (OJST) , 2013, DOI: 10.4236/ojst.2013.31020
Odontomas are defined as hamartomas of odontogenic origin. They are composed of all dental tissues are represented, occurring in a more or less disorderly pattern. Intraosseous (central) odontomas are the odontogenic tumors of greatest incidence. Otherwise, odontomas arising in the extraosseous soft tissue, also known as peripheral odontoma, are extremely uncommon. This article presents a case of peripheral odontoma in a child referred to SNUDH for treatment. A 2-year-old girl was referred to The SNUDH regarding an asymptomatic nodule on the left maxillary buccal gingiva. Clinical examination revealed a 7 mm × 6 mm × 3 mm, sessile mucosal lesion on the buccal gingival between #62 and #63. The lesion was firm on palpation and covered with an intact non-ulcerated mucosa. There was no other mucosal pathology. Under N2O-O2 inhalation sedation, an excisional biopsy of the lesion was performed. The diagnosis was peripheral odontoma. Summary: Odontoma in an extraosseous location represents a challenge for diagnosis. This article reports a case of peripheral odontoma and its clinical presentation, histological evaluation and treatment. A 2-year-old girl reported a firm asymptomatic nodule on left maxillary buccal gingival. The procedures for diagnosis included intraoral examination, excisional biopsy and histological analysis. The diagnosis was peripheral odontoma. Peripheral odontoma is rare and the differential diagnosis with other gingival masses is rather difficult and must include inflammatory and reactive processes. The definitive diagnosis is based on microscopic features.
The Effects of Sleep Deprivation on the Biophysical Properties of Facial Skin  [PDF]
Min Ah Kim, Eun Joo Kim, Byung Young Kang, Hae Kwang Lee
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2017, DOI: 10.4236/jcdsa.2017.71004
Abstract: Lack of sleep is a problem in today’s society, and many people are concerned about changes in their outward appearance due to lack of sleep. People generally come up with some noticeable skin attributes as symptoms of sleep deficiency including rough, dull, and dry skin as well as droopy eyelids and dark eye circles. Several previous reports also suggested that poor sleep could affect the skin condition. The purpose of this study was to evaluate the effect of one night of sleep deprivation on various skin biophysical properties. Twenty four healthy females participated in a study of one night of sleep deprivation. Subjects were kept awake for one night in a laboratory with controlled temperature and relative humidity. The skin condition of each subject was evaluated after normal sleep pattern and after one night of sleep deprivation. The measured skin biophysical parameters included transepidermal water loss, facial pore size, and skin tone, hydration, elasticity, desquamation, translucency, and blood flow. The cheek, eye, and lip areas were evaluated. After one night of sleep deprivation, multiple skin biophysical parameters showed changes when compared to the baseline measurements. A significant decrease in skin hydration and impaired barrier function were observed (p < 0.05). Decreased hydration led to decreased skin elasticity and translucency and increased skin scaling (p < 0.05). Facial pores were more conspicuous, and skin lightness decreased significantly (p < 0.05). Furthermore, skin blood flow decreased prominently (p < 0.05). The results demonstrated that the skin features that are recognized as symptoms of sleep deficiency actually showed remarkable differences after a period of sleep deprivation, and some of these features were confirmed in the eye, lip, as well as the cheek areas. This study revealed a significant association between sleep deprivation and skin biophysical properties by scientific measuring.
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