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The epidemiology and prevalence of Ulcerative colitis in the South of India  [PDF]
Sivaram Gunisetty, Santosh Kumar Tiwari, Avinash Bardia, Meka Phanibhushan, Vishnupriya Satti, Mohammed Aejaz Habeeb, Aleem Ahmed Khan
Open Journal of Immunology (OJI) , 2012, DOI: 10.4236/oji.2012.24018
Abstract: Ulcerative colitis is considered frequent in majority of European and North American population and exceptional in most of the developing Asian countries. There is a dearth of reported data from South India on the incidence of the disease and its prevalence. Hence the present study was designed to estimate the prevalence of ulcerative colitis in a tertiary care hospital of Hyderabad, South India. The study population consisted of 157 Ulcerative colitis and 204 healthy subjects. All subjects were interviewed by means of a questionnaire for general demographical details and socioeconomic conditions, health related quality of life and history of UC. Patients were categorized based on disease severity; moderate: 95, and severe: 62 and disease manifestation: 73 (46.5%) pancolitis, 60 (38.2%) left-sided colitis and 24 (15.3%) had proctosigmoiditits. Disease prevalence was high in patients of <35 years age. Junk/outside food consumers were significantly high in Ulcerative colitis than controls 68 (43.3%) vs 67 (32.8%) p = 0.048. There was no significant difference of disease prevalence with diet, drinking habits and alcoholic consumption. Whereas UC prevalence was high in nonsmokers than smokers (p = 0.025). This report establishes the importance of various factors in ulcerative colitis. This is the first population based study from South India that reports the prevalence of ulcerative colitis. Ulcerative colitis is more predominant in young age. Further, junk food consumers and Non-smokers/ex-smokers were found to be high in terms of UC prevalence.
A study on the transmission of helicobacter pylori from food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples  [PDF]
Khawaja Shakeel Ahmed, Basheer Madompoyil, Janak Dulari Ahi, Aleem Ahmed Khan, Santosh Kumar Tiwari, Mohammed Aejaz Habeeb
Health (Health) , 2014, DOI: 10.4236/health.2014.64040
Abstract:

Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in 1000 people across India. The Ratio of Male: female was 700: 300 with age group ranging from 20 - 39. Three gastric biopsies were collected for identification of Helicobacter pylori. PCR (Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis showed the prevalence of H. pylori as 70.8% in individuals frequently consuming food prepared under less hygienic condition (street vendor’s food) whereas 60% (less prevalence) in individuals consuming food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s food is not directly involved in the transmission of this bacterium. The unhygienic preparation of food and poor personal hygiene play a vital role in the transmission of these bacteria.

Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model
Nyamathulla Parveen,Aleem Ahmed Khan,Subramani Baskar,Mohammed Aejaz Habeeb
Hepatitis Monthly , 2008,
Abstract: Orthotopic liver transplantation (OLT) is the only treatment that improves the survival rate in patients with acute liver failure (ALF). Hepatocytes are anchorage dependent and require a substratum for their long-term survival. Various substrata are being used to improve the function and survival of these hepatocytes, but cannot be used clinically as they are animal derived. This has been overcome by thermoreversible gelation polymer (TGP). In the present study, rat hepatocytes embedded in TGP, were transplanted intraperitoneally in acute liver failure rat model. Efficacy of the transplanted cells was studied by assessing biochemical parameters and histopathology at different time points. The ALF condition reverted to normal 15 days after cell transplantation. The transplanted animals survived 100%. Therefore this study suggests that the cells in TGP provide substratum to the transplanted cells for long term survival and could successfully provide liver support in severe ALF.
Characterization of hepatic progenitors from human fetal liver during second trimester
Mekala Subba Rao, Aleem Ahmed Khan, Nyamath Parveen, Mohammed Aejaz Habeeb, Chittoor Mohammed Habibullah, Gopal Pande
World Journal of Gastroenterology , 2008,
Abstract: AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule (EpCAM) as a marker from human fetal liver and investigate the expression of human leukocyte antigen (HLA) and their markers associated with hepatic progenitor cells.METHODS: EpCAM +ve cells were isolated using magnetic cell sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 (integrin β1), CD49f (integrin α6) and CD90 (Thy 1) was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class I (A, B, C) and class II (DR) expression was studied by flow cytometry only.RESULTS: FACS analysis indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA class I, albumin and AFP but negative for HLA class II (DR) and CD45. RT PCR showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical staining, EpCAM +ve cells were shown positive signals for CK18 and albumin.CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor.
Association of Helicobacter pylori restriction endonuclease-replacing gene, hrgA with overt gastrointestinal diseases
G, Manoj;Tiwari, Santosh K.;Sharma, Vishwas;Habeeb, Mohammed Aejaz;Khan, Aleem A.;Cm, Habibullah;
Arquivos de Gastroenterologia , 2008, DOI: 10.1590/S0004-28032008000300011
Abstract: background and aim: helicobacter pylori has been proven to be responsible for causing various gastrointestinal disorders including gastric adenocarcinoma. several genes of pathogen (the genes of the cag-pai, vaca, icea, and baba) either in combination or independently have been reported to significantly increase the risk of ulceration/gastric carcinoma, with the caga gene having the strongest predictive value. pursuit to identify new genes which could serve as a marker of overt disease progression, lead to the discovery of hrga gene. methods: fifty-six indigenous strains of h. pylori from subjects with various gastric disorder were screened to assess the status of hrga gene along with the caga gene using simple polymerase chain reaction using specific oligonucleotide primers. post-amplification, amplicons were subjected for sequencing to identify any strain specific variations in sequences from the h. pylori isolated from different disease manifestations. histopathological analysis was done to ascertain any significant change in the histological scores of subjects infected with caga+/hrga+ and caga-/hrg+ strains. results: all the 56 (100%) subjects amplified with the oligonucleotide primers specific to hrga gene, whereas 81.71% subjects showed the presence of caga gene. sequencing of the amplimers showed 99% homology. histology of the caga+/hrga+ and caga-/hrg+ subjects did not show any significant difference. conclusion: hrga gene of helicobacter pylori is not a ideal surrogate marker for identifying individuals with higher risk of developing overt gastro-duodenal diseases such as neoplasia of the stomach.
Seroepidemiology of hepatitis A virus in Hyderabad, South India
Rahamathulla Syed,Aejaz Habeeb Mohammed,Prathap Kumar Sindiri,Arif Aziz Nathani
Journal of Medical and Allied Sciences , 2012,
Abstract: The present study was carried to determine the prevalence of Hepatitis A virus (HAV) IgM antibody among general population in Hyderabad since January 2002 to December 2011. A total of 4571 subjects were selected and divided into groups on the basis of age. The serum samples were screened for the presence of HAV IgM antibodies (anti-HAV IgM) using a commercially available enzyme linked immunosorbent assay (ELISA) kit. Of the 4571 serum samples, 4257 were negative and 314 (6.87%) were positive for the HAV IgM assay. The disease predominance was more in males (212) with 67.51% compared to females (102) with 32.48%. Anti-HAV seroprevalence was measured for the following age groups: Early (≤20 years), Middle (21-40 years) and Late (≥41 years). Average age-wise IgM-anti-HAV positivity from 2002-2011 was 234 of 314 in ≤20 years, 74.52%), 73 of 314 (21-40 years, 23.24%), and 7 of 314 (≥41 years, 2.22%). The present study will provide insights to the recent epidemiologic features of HAV infection in South India.
Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies
Mohammed Ibrahim, Mohammed Nane Khaja, Anjum Aara, Aleem Ahmed Khan, Mohammed Aejaz Habeeb, Yalavarthy Prameela Devi, Mangamoori Lakshmi Narasu, Chitoor Mohammed Habibullah
World Journal of Gastroenterology , 2008,
Abstract: AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats.METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats.RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo: extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities.CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury.
Application of Scale Relativity (ScR) Theory to the Problem of a Particle in a Finite One-Dimensional Square Well (FODSW) Potential  [PDF]
Saeed N. T Al-Rashid, Mohammed A. Z Habeeb, Khalid A Ahmad
Journal of Quantum Information Science (JQIS) , 2011, DOI: 10.4236/jqis.2011.11002
Abstract: In the present work, and along the lines of Hermann, ScR theory is applied to a finite one-dimensional square well potential problem. The aim is to show that scale relativity theory can reproduce quantum mechanical results without employing the Schrödinger equation. Some mathematical difficulties that arise when obtaining the solution to this problem were overcome by utilizing a novel mathematical connection between ScR theory and the well-known Riccati equation. Computer programs were written using the standard MATLAB 7 code to numerically simulate the behavior of the quantum particle in the above potential utilizing the solutions of the fractal equations of motion obtained from ScR theory. Several attempts were made to fix some of the parameters in the numerical simulations to obtain the best possible results in a practical computer CPU time within limited local computer facilities [1,2]. Comparison of the present results with the corresponding results obtained from conventional quantum mechanics by solving the Schrödinger equation, shows very good agreement. This agreement was improved further by optimizing the parameters used in the numerical simulations [1,3]. This represents a new example where scale relativity theory, based on a fractal space-time concept, can accurately reproduce quantum mechanical results without invoking the Schrödinger equation.
Qualitative Interpretation of Gravity and Aeromagnetic Data in West of Tikrit City and Surroundings, Iraq  [PDF]
Emad Mohammed Salah Al-Heety, Mahmoud Abdullah Al-Mufarji, Losyan Habeeb Al Esho
International Journal of Geosciences (IJG) , 2017, DOI: 10.4236/ijg.2017.82005
Abstract: The qualitative interpretation of anomalies of the gravity and the magnetic fields at the west of Tikrit City and surroundings, Iraq, was carried out. According to the tectonic framework of Iraq, the study area is classified as a part of the stable shelf unit. The polynomial technique was employed to separate the residual-regional anomalies as a first step in the qualitative interpretation. Bouguer gravity anomaly map did not exhibit any clear closures. The magnetic maps showed remarkable high closure in the central eastern part of the study area. The regional gravity and magnetic fields values decline towards the eastern parts of the maps (Mesopotamian Basin) in consistent with the increase in the sedimentary cover (basement depth). The residual gravity and magnetic maps displayed several positive and negative closures. The gravity and magnetic lineaments were depicted and superimposed on the tectonic map of the study area. The rose diagram showed that the dominant orientation of the gravity and magnetic lineaments was toward NE-SW.
Application of Scale Relativity to the Problem of a Particle in a Simple Harmonic Oscillator Potential  [PDF]
Saeed N. T. Al-Rashid, Mohammed A. Z. Habeeb, Khalid A. Ahmed
Journal of Quantum Information Science (JQIS) , 2017, DOI: 10.4236/jqis.2017.73008
Abstract: In the present work, Scale Relativity (SR) is applied to a particle in a simple harmonic oscillator (SHO) potential. This is done by utilizing a novel mathematical connection between SR approach to quantum mechanics and the well-known Riccati equation. Then, computer programs were written using the standard MATLAB 7 code to numerically simulate the behavior of the quantum particle utilizing the solutions of the fractal equations of motion obtained from SR method. Comparison of the results with the conventional quantum mechanics probability density is shown to be in very precise agreement. This agreement was improved further for some cases by utilizing the idea of thermalization of the initial particle state and by optimizing the parameters used in the numerical simulations such as the time step and number of coordinate divisions. It is concluded from the present work that SR method can be used as a basis for description the quantum behavior without reference to conventional formulation of quantum mechanics. Hence, it can also be concluded that the fractal nature of space-time implied by SR, is at the origin of the quantum behavior observed in these problems. The novel mathematical connection between SR and the Riccati equation, which was previously used in quantum mechanics without reference to SR, needs further investigation in future work.
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