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Search Results: 1 - 10 of 7791 matches for " Adam Warner "
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Determining the Sub-Cellular Localization of Proteins within Caenorhabditis elegans Body Wall Muscle
Barbara Meissner,Teresa Rogalski,Ryan Viveiros,Adam Warner,Lorena Plastino,Adam Lorch,Laure Granger,Laurent Segalat,Donald G. Moerman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019937
Abstract: Determining the sub-cellular localization of a protein within a cell is often an essential step towards understanding its function. In Caenorhabditis elegans, the relatively large size of the body wall muscle cells and the exquisite organization of their sarcomeres offer an opportunity to identify the precise position of proteins within cell substructures. Our goal in this study is to generate a comprehensive “localizome” for C. elegans body wall muscle by GFP-tagging proteins expressed in muscle and determining their location within the cell. For this project, we focused on proteins that we know are expressed in muscle and are orthologs or at least homologs of human proteins. To date we have analyzed the expression of about 227 GFP-tagged proteins that show localized expression in the body wall muscle of this nematode (e.g. dense bodies, M-lines, myofilaments, mitochondria, cell membrane, nucleus or nucleolus). For most proteins analyzed in this study no prior data on sub-cellular localization was available. In addition to discrete sub-cellular localization we observe overlapping patterns of localization including the presence of a protein in the dense body and the nucleus, or the dense body and the M-lines. In total we discern more than 14 sub-cellular localization patterns within nematode body wall muscle. The localization of this large set of proteins within a muscle cell will serve as an invaluable resource in our investigation of muscle sarcomere assembly and function.
A Pipeline to Determine RT-QPCR Control Genes for Evolutionary Studies: Application to Primate Gene Expression across Multiple Tissues
Olivier Fedrigo,Lisa R. Warner,Adam D. Pfefferle,Courtney C. Babbitt,Peter Cruz-Gordillo,Gregory A. Wray
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0012545
Abstract: Because many species-specific phenotypic differences are assumed to be caused by differential regulation of gene expression, many recent investigations have focused on measuring transcript abundance. Despite the availability of high-throughput platforms, quantitative real-time polymerase chain reaction (RT-QPCR) is often the method of choice because of its low cost and wider dynamic range. However, the accuracy of this technique heavily relies on the use of multiple valid control genes for normalization. We created a pipeline for choosing genes potentially useful as RT-QPCR control genes for measuring expression between human and chimpanzee samples across multiple tissues, using published microarrays and a measure of tissue-specificity. We identified 13 genes from the pipeline and from commonly used control genes: ACTB, USP49, ARGHGEF2, GSK3A, TBP, SDHA, EIF2B2, GPDH, YWHAZ, HPTR1, RPL13A, HMBS, and EEF2. We then tested these candidate genes and validated their expression stability across species. We established the rank order of the most preferable set of genes for single and combined tissues. Our results suggest that for at least three tissues (cerebral cortex, liver, and skeletal muscle), EIF2B2, EEF2, HMBS, and SDHA are useful genes for normalizing human and chimpanzee expression using RT-QPCR. Interestingly, other commonly used control genes, including TBP, GAPDH, and, especially ACTB do not perform as well. This pipeline could be easily adapted to other species for which expression data exist, providing taxonomically appropriate control genes for comparisons of gene expression among species.
Multi-stakeholder platforms: integrating society in water resource management?
Warner, Jeroen;
Ambiente & Sociedade , 2005, DOI: 10.1590/S1414-753X2005000200001
Abstract: multi-stakeholder platforms are a currently popular concept in the international water world. it is however not a very well defined phenomenon. the present article unpacks the concept, proposes to see platforms as networks, and identifies two ′schools of thought′: social learning and negotiation. it attempts a preliminary typology of platforms encountered in real life, in which the comités de bacia in brazil, for all their shortcomings, come out as a relatively influential type. in closing, the article then identifies reasons for non-participation, suggesting that it is an inevitable corollary of organised participation.
Facilitation strategies to improve the use of evidence in integrated stroke care
Grace Warner
International Journal of Integrated Care , 2008,
Kommunale Versorgung, Verortung und Steuerung von Risiken psychischer Gesundheit Community Care and the Location and Governance of Risk in Mental Health La asistencia comunitaria y la localización y gobierno del riesgo en salud mental
Joanne Warner
Forum : Qualitative Social Research , 2006,
Abstract: Das Risikokonzept ist heute zentral für alle Bereiche von gesundheitlicher und sozialer Wohlfahrt in Gro britannien. Im Bereich psychischer Gesundheit und insbesondere seit den Ma nahmen zur kommunalen Versorgung in den 1990er Jahren kommt dem Konzept jedoch besondere Aufmerksamkeit zu. Dabei kamen bisher vor allem die Risiken in den Blick, die von Personen mit psychischen Erkrankungen oder in psychischer Not ausgehen, aber nicht die Risiken, mit denen diese selbst konfrontiert sind. Der vorliegende Beitrag beruht auf qualitativem Material, das mit Hilfe teilstandardisierter Interviews mit 39 Sozialarbeitern in England erhoben wurde, und entwickelt einen theoretischen Rahmen für die Risiken im Kontext des Dienstes für geistige Gesundheit. Anhand der Daten wird illustriert, dass sich die Bedeutung von Risiken für die Politik und die professionelle Praxis mit drei grundlegenden Konzepten fassen l sst: "gef hrliche Personen", "(psychotische) Erkrankung" und "soziale Kontexte". Zum einen verorteten Sozialarbeiter Risiken in "gef hrlichen Personen". Dabei stellte der junge schwarze Mann mit einer Schizophreniediagnose den Idealtypus eines gef hrlichen Individuums dar. Zudem wurden Risiken aber auch innerhalb einer Person verortet bzw. auf eine bestimmte Erkrankung bezogen. Schlie lich wurden Risiken sozialen Kontexten und spezifischen Umst nden zugeordnet, die gef hrliches Verhalten, so die befragten Sozialarbeiter, f rdern. Abschlie end wird gezeigt, wie der entwickelte theoretische Rahmen in anderen Kontexten nutzbringend angewendet werden kann. URN: urn:nbn:de:0114-fqs0601310 The concept of risk is now central to all areas of health and social welfare in the UK, although its exact character in relation to different groups varies. It has been argued that risk in mental health has been characterised by a preoccupation with the perceived risk of violence to others posed by those experiencing mental distress, particularly since the implementation of community care policies in the 1990s. The present paper draws on qualitative materials from semi-structured interviews with thirty-nine mental health social workers to demonstrate the significance for policy and practice of identifying where professionals see risk as being located. In the present study, three key sites were identified: firstly, risk was located in dangerous individuals, where the concept "high-risk" was particularly closely identified with young Black men with a diagnosis of schizophrenia. Secondly, social workers located risk in within-subject entities such as active psychotic illness, when it was
Science in Wonderland
Brian Warner
South African Journal of Science , 2010, DOI: 10.4102/sajs.v106i1/2.109
Charles Darwin and John Herschel
B. Warner
South African Journal of Science , 2010, DOI: 10.4102/sajs.v105i11/12.147
Abstract: The influence of John Herschel on the philosophical thoughts of Charles Darwin, both through the former’s book, Natural Philosophy, and through their meeting in 1836 at the Cape of Good Hope, is discussed. With Herschel having himself speculated on evolution just a few months before he met Darwin, it is probable that he stimulated at least the beginnings of the latter’s lifelong work on the subject.
The Right to Oblivion: Data Retention from Canada to Europe in Three Backward Steps
Jeremy Warner
University of Ottawa Law and Technology Journal , 2005,
Abstract: [] The issue of data retention is one that has become prominent in recent times, particularly with the recent extension of Canadian privacy legislation to cover the private sector. This paper investigates the origins of the prohibition on data retention under European and Canadian law and its subsequent development in Europe and Canada with an emphasis on the trends, disparities and other consequences generated by the prohibition since 1968, the date of the first Council of Europe recommendations in relation to data protection in general. In Europe, ever since the first proposal for harmonized data protection laws was made by the Council of Europe in 1973, one of the fundamental principles of data protection law has been that of data retention or data conservation – that is, the obligation of the data user or controller to keep data for a limited period of time only. The 1995 EU Directive on Data Protection contains an express data-retention principle. The OECD Guidelines, which were used to develop Canada’s privacy standard and subsequent privacy legislation, are less explicit. In Canada, Part 1 of the Personal Information Protection and Electronic Documents Act (PIPEDA) establishes Principle 5 on data retention or data conservation, which is closely allied with its European counterpart. The connections between each of these discrete legal instruments are obscured by the legal backgrounds to each of PIPEDA and the EU Directive. The paper examines the data-retention principle under PIPEDA, analyzing the extent to which this principle has been influenced by the European legal developments and the extent to which other factors were important in shaping this fundamental rule.***** [Résumé] La question de la rétention des données est devenue un sujet de l’heure récemment, en particulier avec l’extension récente de la loi canadienne relative à la protection de la vie privée au secteur privé. Cet article examine les origines de la prohibition de la rétention des données en droit européen et canadien et son évolution subséquente en Europe et au Canada, en insistant sur les tendances, les disparités et les autres conséquences de cette prohibition depuis 1968, date à laquelle le Conseil de l’Europe a fait ses premières recommandations relativement à la protection des données en général. En Europe, depuis que le Conseil de l’Europe a proposé pour la première fois en 1973 l’harmonisation des lois relatives à la protection des données, l’un des principes fondamentaux de cette protection est la rétention ou la conservation des données, c’est-à-dire l’obligation que l
Contested hydrohegemony: Hydraulic control and security in Turkey
Jeroen Warner
Water Alternatives , 2008,
Abstract: The article seeks to expand the understanding of the emerging concept of hydrohegemony (Zeitoun and Warner, 2006). Illustrated by Turkey’s strategy with respect to the Euphrates-Tigris it looks at the layered nature of water-related political strategies at different levels. The article therefore introduces hegemony as a layered phenomenon whose multi-level interactions impinge on each other. It zooms in on Turkish hegemony in its hydraulic control and security strategies, and the international repercussions of that strategy.The present analysis suggests that Turkey’s basin and regional hegemony is contested and constrained from different sides, not least at home. Its water projects are a flashpoint of domestic, basin as well as global politics. It argues that the need to access capital in the international market to realise these ambitions necessitated a 'passive revolution' in Turkey which opened a window of opportunity utilized by the internationalised counter-hegemonic moves against Turkey’s dam projects in Southeast Anatolia, notably the ongoing Ilisu dam on the Tigris.
Forms of labour in information systems
Julian Warner
Information Research: an international electronic journal , 2002,
Abstract: A presentation open to further development is given. The idea of technology, including information technology, as a human construction is taken as the basis for the themes to be developed. The possibility of constructing an information dynamic, continuous with the dynamic of capitalism, is considered. Differentiations are made between forms of semiotic labour: semantic from syntactic labour and communal from universal labour. Information retrieval systems and the departure from the labour theory of copyright are considered in relation to the forms of labour distinguished. An information dynamic is constructed. The potential and limitations of syntactic labour are considered. The analytic value of the distinctions developed is differentiated from the possible predictive power of the dynamic indicated. The Semantic Web is viewed from the perspective of these considerations.
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