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Search Results: 1 - 10 of 935 matches for " Adalberto águila "
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Estudio de marcadores fenotípicos y de susceptibilidad antimicrobiana en cepas de Escherichia coli entéricas Study of phenotypical and antimicrobial susceptibility markers in enteric Escherichia coli strains
Adalberto águila,Robert Bernedo,Alina Llop,Margarita Ramírez
Revista Cubana de Medicina Tropical , 2007,
Abstract: Se analizaron 41 cepas de Escherichia coli, aisladas en ni os menores de 5 a os con diarreas agudas, procedentes de las diferentes provincias del país. Fueron probados 4 importantes determinantes fenotípicos: sorbosa, sorbitol, enterohemolisina y la serología de O157: H7, para seleccionar las cepas de la categoría enterohemorrágica o productora de toxina shiga. De igual modo fueron caracterizadas por el método de biotipaje y de susceptibilidad antimicrobiana. El uso de las pruebas fenotípicas mostró 6 cepas con características presuntivas, de estas, 4 mostraron mayor probabilidad de pertenecer a la categoría productora de toxina shiga. En el ensayo de susceptibilidad antimicrobiana las cepas mostraron elevada resistencia, sobre todo para la ampicilina y el trimetropin-sulfametoxasol. Otro hallazgo de interés fueron los valores de resistencia y de susceptibilidad intermedia obtenidos para el augmentin, aztreonan y ceftriaxona. Se alcanzaron 12 patrones de resistencia antimicrobiana y de ellos 10 resaltaron por ser multirresistentes. Forty strains of Escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. Four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157: H7 serology, in order to select those strains from enterohemorrhagic or Shiga toxin-producing category. Likewise, they were characterized by biotyping and antimicrobial susceptibility methods. The use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be Shiga toxin-producing strains. In antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. Another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. There were 12 antimicrobial resistance patterns of which 10 were multi-resistant.
Estudio de correlación entre cepas Escherichia coli sorbosa negativa y su capacidad de producir enterotoxinas
águila,Adalberto; Valdés-Dapena,Margarita; Ramírez,Margarita; Fernández,Carlos; Bravo,Laura;
Revista Cubana de Medicina Tropical , 2006,
Abstract: fifty negative sorbose escherichia coli strains isolated from children who suffered spontaneously occurring fluid diarrheas were studied. identification up to the level of species was performed by conventional methods including phenotypical markers known as sorbose and sorbitol. the pcr and dna gene hybridization were applied to confirm the enterotoxin-producing capacity. a good correlation between negative sorbose escherichia coli and their capacity of producing heat-labile and heat -stable enterotoxin was observed. the study showed a high percentage of these strains with encoding gene fragments, 94 and 44% respectively, being heat-stable enterotoxin the predominant. the main primary biotypes of taxonomic and epidemiological importance were obtained. it was demonstrated that the sorbose method has certain qualities that make it stand out as a phenotypical marker, with 100% sensitivity and 88% specificity, and as a practical presumptive diagnostic method that is very useful for clinical microbiology laboratories.
Estudio de marcadores fenotípicos y de susceptibilidad antimicrobiana en cepas de Escherichia coli entéricas
águila,Adalberto; Bernedo,Robert; Llop,Alina; Ramírez,Margarita; Bravo,Laura; Fernandez,Anabel; Ledo,Yudith;
Revista Cubana de Medicina Tropical , 2007,
Abstract: forty strains of escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157: h7 serology, in order to select those strains from enterohemorrhagic or shiga toxin-producing category. likewise, they were characterized by biotyping and antimicrobial susceptibility methods. the use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be shiga toxin-producing strains. in antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. there were 12 antimicrobial resistance patterns of which 10 were multi-resistant.
Estudio de correlación entre cepas Escherichia coli sorbosa negativa y su capacidad de producir enterotoxinas Study of correlation between negative sorbose E. coli strains and their enterotoxin-producing capacity
Adalberto águila,Margarita Valdés-Dapena,Margarita Ramírez,Carlos Fernández
Revista Cubana de Medicina Tropical , 2006,
Abstract: Se estudiaron 50 cepas de Escherichia coli sorbosa negativas, aisladas de ni os con diarreas líquidas de aparición espontánea. La identificación hasta especie se realizó por métodos convencionales, incluidos los marcadores fenotípicos sorbosa y sorbitol. Las técnicas de reacción en cadena de la polimerasa e hibridación de ADN, fueron realizadas para confirmar la capacidad de producción de enterotoxinas. Se obtuvo muy buena correlación entre las cepas de Escherichia coli sorbosa negativas y su capacidad de elaborar enterotoxinas termolábil y termoestable. El estudio demostró un alto porcentaje de estas cepas con fragmentos de genes codificadores, 94 y 44 % respectivamente, predominando la enterotoxina termoestable. Se obtuvieron los principales biotipos primarios de importancia taxonómica y epidemiológica. Se demostró que el método de la sorbosa posee cualidades que lo distinguen como marcador fenotípico, con 100 % de sensibilidad y 88 % de especificidad, revelándose como método de diagnóstico presuntivo práctico y de gran utilidad para los Laboratorios de Microbiología Clínica. Fifty negative sorbose Escherichia coli strains isolated from children who suffered spontaneously occurring fluid diarrheas were studied. Identification up to the level of species was performed by conventional methods including phenotypical markers known as sorbose and sorbitol. The PCR and DNA gene hybridization were applied to confirm the enterotoxin-producing capacity. A good correlation between negative sorbose Escherichia coli and their capacity of producing heat-labile and heat -stable enterotoxin was observed. The study showed a high percentage of these strains with encoding gene fragments, 94 and 44% respectively, being heat-stable enterotoxin the predominant. The main primary biotypes of taxonomic and epidemiological importance were obtained. It was demonstrated that the sorbose method has certain qualities that make it stand out as a phenotypical marker, with 100% sensitivity and 88% specificity, and as a practical presumptive diagnostic method that is very useful for Clinical Microbiology Laboratories.
Aeromonas spp asociada a enfermedad diarreica aguda en Cuba: estudios de casos y controles
Bravo,Laura; Fernández,Anabel; Nú?ez,Fidel á; Rivero,Luis A; Ramírez,Margarita; águila,Adalberto; Ledo,Yudith; Cruz,Yanaika; Hernández,Jenny;
Revista chilena de infectología , 2012, DOI: 10.4067/S0716-10182012000100008
Abstract: the members of the genus aeromonas are currently considered important gastrointestinal pathogens in different geographical areas. from february 1985 to january 2005 several case-control studies were coordinated by the national reference laboratory for diarrheal diseases from the pedro kouri institute. the study purpose was to analyze a possible pathogenic role for aeromonas spp in cuban children with acute diarrhea. in that period 2,322 children less than 5 years old with acute diarrhea were studied for diarhoeal pathogens and another group of 2,072 non hospitalized children without diarrhea during the similar time from the same geographical areas and matched by ages were recruited. in the group of children with diarrheas (cases), aeromonas spp. was isolated in 166 (7.15%) and in the control group the microorganism was found in only 35 (1.76%). when aeromonas isolation rates were compared between both groups, we found that probability to isolate this specie was significantly higher in cases than in controls (or = 4.48, 95% ic: 3.05-6.60; p < 0.001). the aeromonas species more frequently isolated were a. caviae, a. hydrophila, and a. veronii bv sobria. other enteric pathogens detected in children with diarrhea were: shigella spp in 418 (18%) (p < 0.0001), salmonella spp in 53 (2.3%) (p < 0.01), and enteropathogenic e. coli in 58 (2.49%) (p < 0.05).
Sensibilidad in vitro de cepas de Candida frente a fluconazol y anfotericina B
Fernández Andreu,Carlos M.; Martínez Machín,Gerardo; Illnait Zaragozí,María T.; Perurena Lancha,Mayda R.; águila Sánchez,Adalberto; Brito Galloso,Miguel;
Revista Cubana de Medicina Tropical , 2007,
Abstract: in vitro antifungal susceptibility testing is really necessary at present in medical mycology laboratories. minimal inhibitory concentration of 210 candida yeast strains isolated from hiv/aids patients with oral lesions was determined to find out susceptibility to fluconazole and amphotericin b. a clsi′s m27a2 protocol-based dilution micromethod was used. c. albicans was predominant (62.4 %)followed by c. glabrata, c. parapsilosis, and c. tropicalis. regarding amphotericin b, the mode value and mic50 were 0,125 μg/ml, which indicated the effectiveness of this drug in vitro conditions. only c. krusei strain showed a minimal inhibitory concentration over 1 μg/ml. 8.1 % of strains were resistent to fluconazole whereas 8.1 % was dose- depending susceptible. the less susceptible species were c. krusei and c. glabrata. the achieved results laid the foundations for wider studies to be made in the cuban context and indicated the need of keeping surveillance on the behaviour of clinical yeast isolates to antifungal drugs.
Dictadura y memoria: El conflictivo contrapunto entre las memorias de la dictadura en Rosario
Gabriela águila
Prohistoria , 2007,
Abstract: El artículo explora la relación entre la (o las) memorias de la última dictadura militar (1976-83) y el impacto público y social de ciertas políticas de la memoria en la ciudad de Rosario, así como los debates desplegados en el espacio público y político local en estos últimos a os respecto de la preservación de la memoria del periodo. The article explores the relations between memories of last military dictatorship (1976-83) and the public and social impact of some politics of memory in Rosario, Argentina. In this way, analyzes various debates development in recent years in public and politic space about preservation of memory of this period.
Electrorotation: A Spectroscopic Imaging Approach to Study the Alterations of the Cytoplasmic Membrane  [PDF]
Adalberto Bonincontro, Gianfranco Risuleo
Advances in Molecular Imaging (AMI) , 2015, DOI: 10.4236/ami.2015.51001
Abstract: Electrorotation (ER) is a technique allowing the characterization of the surface properties of a variety of supra-molecular aggregates and living cultured cells as well as cellular organelles and biological materials in general. In particular, this technique allows measuring two important physical parameters of the cell membrane: specific capacitance and specific conductance. These parameters are strictly related to the structure/function relationships of the biological membrane; ER becomes thus a powerful means to investigate a number of phenomena involving the membrane integrity. These phenomena may originate from treatments with exogenous molecules and/or from pathological effects. Concerning these aspects, the study of the transfer of exogenous material (i.e. cat-anionic vesicles or liposomes) across the cell membrane assumes a high importance. This review is focused on the physical functioning principles of ER and on the quantitative analysis of the experimental measurements. This work also reports on different fields of application of ER with particular reference to data obtained in our laboratory. The investigation of the alterations of the cytoplasmic membrane function, as evidenced by this strategy, will be illustrated in detail.
Escravid?o e sociabilidade capitalista: um ensaio sobre inércia social
Cardoso, Adalberto;
Novos Estudos - CEBRAP , 2008, DOI: 10.1590/S0101-33002008000100006
Abstract: based on historiographical studies that since 1980?s have undertaken a broad review of the social history of labor in brazil, the article presents some sociological hypotheses about the permanence of structural features of slavery past in the process of building of the capitalist sociality in the country. this legacy includes a depreciated perception of manual work, a derogatory image of black and even national people as workers, an indifference of the elites toward poor majorities, and an extremely rigid social hierarchy. according to the author, this framework of structural inertia provided the general parameters of free labor?s reproduction in the beginning of capitalist order in brazil.
A inven??o do Brasil como terra do samba: os sambistas e sua afirma??o social
Paranhos, Adalberto;
História (S?o Paulo) , 2003, DOI: 10.1590/S0101-90742003000100004
Abstract: as in a race of obstacles, samba traveled across bumpy paths until it was recognized by legitimization instances of the state as a national symbol. throughout this process, the samba composers and interpreters of urban rio samba played a decisive role. this article searches to clarify some hardly studied aspects of the marches and counter-marches which marked the history of samba and its passage from culturally marginal artifact to the bearer of brazilian singularity in the musical field.
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