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Search Results: 1 - 9 of 9 matches for " Achara Teeraratkul "
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Diagnostic Accuracy of a Loop-Mediated Isothermal PCR Assay for Detection of Orientia tsutsugamushi during Acute Scrub Typhus Infection
Daniel H. Paris ,Stuart D. Blacksell,Pruksa Nawtaisong,Kemajittra Jenjaroen,Achara Teeraratkul,Wirongrong Chierakul,Vanaporn Wuthiekanun,Pacharee Kantipong,Nicholas P. J. Day
PLOS Neglected Tropical Diseases , 2011, DOI: 10.1371/journal.pntd.0001307
Abstract: Background There is an urgent need to develop rapid and accurate point-of-care (POC) technologies for acute scrub typhus diagnosis in low-resource, primary health care settings to guide clinical therapy. Methodology/Principal Findings In this study we present the clinical evaluation of loop-mediated isothermal PCR assay (LAMP) in the context of a prospective fever study, including 161 patients from scrub typhus-endemic Chiang Rai, northern Thailand. A robust reference comparator set comprising following ‘scrub typhus infection criteria’ (STIC) was used: a) positive cell culture isolate and/or b) an admission IgM titer ≥1:12,800 using the ‘gold standard’ indirect immunofluorescence assay (IFA) and/or c) a 4-fold rising IFA IgM titer and/or d) a positive result in at least two out of three PCR assays. Compared to the STIC criteria, all PCR assays (including LAMP) demonstrated high specificity ranging from 96–99%, with sensitivities varying from 40% to 56%, similar to the antibody based rapid test, which had a sensitivity of 47% and a specificity of 95%. Conclusions/Significance The diagnostic accuracy of the LAMP assay was similar to realtime and nested conventional PCR assays, but superior to the antibody-based rapid test in the early disease course. The combination of DNA- and antibody-based detection methods increased sensitivity with minimal reduction of specificity, and expanded the timeframe of adequate diagnostic coverage throughout the acute phase of scrub typhus.
Improved Binding Activity of Antibodies against Major Histocompatibility Complex Class I Chain-Related Gene A by Phage Display Technology for Cancer-Targeted Therapy
Achara Phumyen,Amonrat Jumnainsong,Chanvit Leelayuwat
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/597647
Abstract: Major histocompatibility complex class I chain-related gene A (MICA) is an NKG2D ligand that is over-expressed under cellular stress including cancer transformation and viral infection. High expression of MICA in cancer tissues or patients' sera is useful for prognostic or follow-up markers in cancer patients. In this study, phage display technology was employed to improve antigen-binding activities of anti-MICA monoclonal antibodies (WW2G8, WW6B7, and WW9B8). The 12 amino acid residues in the complementarity determining regions (CDRs) on the V domain of the heavy chain CDR3 (HCDR3) of these anti-MICA antibodies were modified by PCR-random mutagenesis, and phages displaying mutated anti-MICA Fab were constructed. After seven rounds of panning, five clones of phages displaying mutant anti-MICA Fab which exhibited 3–7-folds higher antigen-binding activities were isolated. Two clones of the mutants (phage-displayed mutant Fab WW9B8.1 and phage-displayed mutant Fab WW9B8.21) were confirmed to have antigen-binding specificity for cell surface MICA proteins by flow cytometry. These phage clones are able to recognize MICA in a native form according to positive results obtained by indirect ELISA and flow cytometry. Thus, these phage particles could be potentially used for further development of nanomedicine specifically targeting cancer cells expressing MICA proteins.
Managing Bioplastics Business Innovation in Start Up Phase
Theinsathid,Pornpun; Chandrachai,Achara; Keeratipibul,Suwimon;
Journal of technology management & innovation , 2009, DOI: 10.4067/S0718-27242009000100007
Abstract: fostering innovation in a mature company can often seem like a swim upstream-the needs of the existing business often overwhelm attempts to create something new. mature companies understand that to compete today they need to innovate. but finding sources of innovation while still paying attention to the current business can be a struggle. open innovation has played an important role to drive the new business in 21th century. academic research commercialization may be challenged by the faculty members' academic routines, institutional priorities, and faculty retention. in order to undertake applied research, university scientists must spend a certain amount of time each period to keep up with the latest scientific developments and absorb new ideas. because almost all faculty members have teaching responsibilities, many of them fear that participation in the protection and commercialization of intellectual property rights is time consuming. there are lots of basic researches focuses on polylactic acid (pla), the most promising bioplastics, however that is still far behind the commercialization step. the close innovation system may take longer time to achieve excellence in its technology under fierce competition environment. this study illuminates the complexities and challenges involved in managing innovation toward specific results. these challenges seem to result, in part, from the multidisciplinary nature of r&d work, it associated with risks, uncertainty and non-linear processes. one of the finding in this study is that many of the factors that drive innovative bioplastic industry are derived in collaboration with technology push and demand pull. the study's findings contribute to our understanding of consumer attitudes towards 'green' products such as bioplastics. most critical in this respect is whether such products make economic sense for a company. this study may provide some guidelines to support development of a concrete direction for pla under open innovat
Managing Bioplastics Business Innovation in Start Up Phase Managing Bioplastics Business Innovation in Start Up Phase Managing Bioplastics Business Innovation in Start Up Phase
Pornpun Theinsathid,Achara Chandrachai,Suwimon Keeratipibul
Journal of technology management & innovation , 2009,
Abstract: Fostering innovation in a mature company can often seem like a swim upstream—the needs of the existing business often overwhelm attempts to create something new. Mature companies understand that to compete today they need to innovate. But finding sources of innovation while still paying attention to the current business can be a struggle. Open innovation has played an important role to drive the new business in 21th century. Academic research commercialization may be challenged by the faculty members’ academic routines, institutional priorities, and faculty retention. In order to undertake applied research, university scientists must spend a certain amount of time each period to keep up with the latest scientific developments and absorb new ideas. Because almost all faculty members have teaching responsibilities, many of them fear that participation in the protection and commercialization of Intellectual Property Rights is time consuming. There are lots of basic researches focuses on polylactic acid (PLA), the most promising bioplastics, however that is still far behind the commercialization step. The close innovation system may take longer time to achieve excellence in its technology under fierce competition environment. This study illuminates the complexities and challenges involved in managing innovation toward specific results. These challenges seem to result, in part, from the multidisciplinary nature of R&D work, it associated with risks, uncertainty and non-linear processes. One of the finding in this study is that many of the factors that drive innovative bioplastic industry are derived in collaboration with technology push and demand pull. The study’s findings contribute to our understanding of consumer attitudes towards ‘Green’ products such as bioplastics. Most critical in this respect is whether such products make economic sense for a company. This study may provide some guidelines to support development of a concrete direction for PLA under open innovation atmosphere. Fostering innovation in a mature company can often seem like a swim upstream—the needs of the existing business often overwhelm attempts to create something new. Mature companies understand that to compete today they need to innovate. But finding sources of innovation while still paying attention to the current business can be a struggle. Open innovation has played an important role to drive the new business in 21th century. Academic research commercialization may be challenged by the faculty members’ academic routines, institutional priorities, and faculty retention. In order
Managing Bioplastics Business Innovation in Start Up Phase
Pornpun Theinsathid,Achara Chandrachai,Suwimon Keeratipibul
Journal of technology management & innovation , 2009,
Abstract: Fostering innovation in a mature company can often seem like a swim upstream—the needs of the existing business often overwhelm attempts to create something new. Mature companies understand that to compete today they need to innovate. But finding sources of innovation while still paying attention to the current business can be a struggle. Open innovation has played an important role to drive the new business in 21th century. Academic research commercialization may be challenged by the faculty members’ academic routines, institutional priorities, and faculty retention. In order to undertake applied research, university scientists must spend a certain amount of time each period to keep up with the latest scientific developments and absorb new ideas. Because almost all faculty members have teaching responsibilities, many of them fear that participation in the protection and commercialization of Intellectual Property Rights is time consuming. There are lots of basic researches focuses on polylactic acid (PLA), the most promising bioplastics, however that is still far behind the commercialization step. The close innovation system may take longer time to achieve excellence in its technology under fierce competition environment. This study illuminates the complexities and challenges involved in managing innovation toward specific results. These challenges seem to result, in part, from the multidisciplinary nature of R&D work, it associated with risks, uncertainty and non-linear processes. One of the finding in this study is that many of the factors that drive innovative bioplastic industry are derived in collaboration with technology push and demand pull. The study’s findings contribute to our understanding of consumer attitudes towards ‘Green’ products such as bioplastics. Most critical in this respect is whether such products make economic sense for a company. This study may provide some guidelines to support development of a concrete direction for PLA under open innovation atmosphere.
On the Unicity of Smartphone Applications
Jagdish Prasad Achara,Gergely Acs,Claude Castelluccia
Computer Science , 2015,
Abstract: Prior works have shown that the list of apps installed by a user reveal a lot about user interests and behavior. These works rely on the semantics of the installed apps and show that various user traits could be learnt automatically using off-the-shelf machine-learning techniques. In this work, we focus on the re-identifiability issue and thoroughly study the unicity of smartphone apps on a dataset containing 54,893 Android users collected over a period of 7 months. Our study finds that any 4 apps installed by a user are enough (more than 95% times) for the re-identification of the user in our dataset. As the complete list of installed apps is unique for 99% of the users in our dataset, it can be easily used to track/profile the users by a service such as Twitter that has access to the whole list of installed apps of users. As our analyzed dataset is small as compared to the total population of Android users, we also study how unicity would vary with larger datasets. This work emphasizes the need of better privacy guards against collection, use and release of the list of installed apps.
Equivalent relaxations of optimal power flow
Subhonmesh Bose,Steven H. Low,Thanchanok Teeraratkul,Babak Hassibi
Computer Science , 2014,
Abstract: Several convex relaxations of the optimal power flow (OPF) problem have recently been developed using both bus injection models and branch flow models. In this paper, we prove relations among three convex relaxations: a semidefinite relaxation that computes a full matrix, a chordal relaxation based on a chordal extension of the network graph, and a second-order cone relaxation that computes the smallest partial matrix. We prove a bijection between the feasible sets of the OPF in the bus injection model and the branch flow model, establishing the equivalence of these two models and their second-order cone relaxations. Our results imply that, for radial networks, all these relaxations are equivalent and one should always solve the second-order cone relaxation. For mesh networks, the semidefinite relaxation is tighter than the second-order cone relaxation but requires a heavier computational effort, and the chordal relaxation strikes a good balance. Simulations are used to illustrate these results.
Safety and tolerability of nevirapine-based antiretroviral therapy in HIV-infected patients receiving fluconazole for cryptococcal prophylaxis: a retrospective cohort study
Weerawat Manosuthi, Nopphanath Chumpathat, Achara Chaovavanich, Somnuek Sungkanuparph
BMC Infectious Diseases , 2005, DOI: 10.1186/1471-2334-5-67
Abstract: A retrospective cohort study was conducted among HIV-infected patients who began NVP-based ART between December 2003 and September 2004. Patients were followed up for 6 months. Clinical hepatitis, elevated aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (> 3 times from baseline), and skin rashes were studied.There were 686 patients; 225, 392, and 69 patients in group A, B, and C, respectively. Baseline characteristics including age, previous opportunistic infections, use of antituberculous drugs, and baseline aminotransferase levels among the three groups were similar. Group C had a higher proportion of men (p = 0.016). Baseline median (IQR) CD4 cell counts were 85 (21–159), 18 (7–48), and 16 (5–35) cell/mm3 in group A, B, and C, respectively (p < 0.001). Of 2/225 (0.9%), 4/392 (1.0%), and 0/69 (0%) patients in group A, B, and C developed clinical hepatitis (p = 0.705). There were no significant difference of elevated AST or ALT among the three groups (p > 0.05). By logistic regression, receiving fluconazole was not predictive of clinical hepatitis, elevated aminotransferase, or skin rashes. At 6 months after initiating NVP, 174 (77.3%) patients in group A, 309 (78.8%) patients in group B, and 58 (84.1%) patients in group C remained on NVP.Initiation of NVP-based ART among Thais with advance HIV disease receiving fluconazole is safe and well-tolerated. nevirapine should not be contraindicated for patients receiving fluconazole for treatment or prophylaxis of cryptococcosis.Highly active antiretroviral therapy (HAART) has been widely used for the treatment of human immunodeficiency virus (HIV) infected patients with successful immune restoration and reductions in morbidity and mortality [1,2]. However, access to antiretroviral therapy for HIV-infected patients in resource-limited countries is still a major obstacle [3,4]. HIV-infected patients in these areas often presented with advanced HIV disease. Nevirapine (NVP) is a non-nucleoside reverse tr
Correlation between PFGE Groups and mrp/epf/sly Genotypes of Human Streptococcus suis Serotype 2 in Northern Thailand
Prasit Tharavichitkul,Kanreuthai Wongsawan,Naoki Takenami,Sumalee Pruksakorn,Achara Fongcom,Marcelo Gottschalk,Banyong Khanthawa,Volaluk Supajatura,Shinji Takai
Journal of Pathogens , 2014, DOI: 10.1155/2014/350416
Abstract: Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig’s blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE) groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4%) and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp+epf?sly? and only 12.9% were in mrp?epf?sly+ genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp+epf?sly? genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2. 1. Introduction Streptococcus suis, recognized as a significant swine and human pathogen, mainly causes meningitis, sepsis, endocarditis, and septic shock [1]. It can be transmitted to humans by contact with sick or carrier pigs, pig-derived products [2], or eating undercooked pork [3, 4]. Capsular polysaccharide (CPS) is the most important proven critical virulence factor, due to its antiphagocytosis activity [5]. Of 35 serotypes, serotype 2 is the most frequently isolated and associated with disease in both animals and humans [6]. In addition, virulence-related proteins, such as muramidase-released protein (MRP), extracellular factor (EF), and hemolysin (suilysin, SLY), are expressed by some strains of S. sui “as discussed by Gottschalk and Segura [1].” These proteins are encoded by the genes mrp, epf, and sly, respectively. MRP/EF/SLY phenotypes or mrp/epf/sly genotypes of S. suis serotype 2 have been studied mostly in pig isolates with very little data for human isolates [1, 7], especially in Northern Thailand. Pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI has been confirmed to be valuable for evaluating the genetic diversity of S. suis [8]. The present study aims to clarify the correlation between PFGE and mrp/epf/sly genotypes of S. suis serotype 2 isolated from patients in Northern Thailand. 2. Materials and Methods 2.1. S. suis Serotype 2 Strains from Humans and Healthy Pigs A total of 66 S. suis serotype 2 (SS2)
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