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Search Results: 1 - 6 of 6 matches for " Acardi "
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Sex pheromone and period gene characterization of Lutzomyia longipalpis sensu lato (Lutz & Neiva) (Diptera: Psychodidae) from Posadas, Argentina
Salomón, Oscar D;Araki, Alejandra S;Hamilton, James GC;Acardi, Soraya A;Peixoto, Alexandre A;
Memórias do Instituto Oswaldo Cruz , 2010, DOI: 10.1590/S0074-02762010000700016
Abstract: lutzomyia longipalpis s.l. is the primary vector of leishmania (l.) infantum in the new world. in this study, male lutzomyia longipalpis specimens from posadas, argentina were characterized for two polymorphic markers: the male sex pheromone and the period (per) gene. the male sex pheromone was identified as (s)-9-methylgermacrene-b, the same compound produced by lu. longipalpis from paraguay and many populations from brazil. the analysis of per gene sequences revealed that the population from argentina is significantly differentiated from previously studied brazilian populations. marker studies could contribute to the understanding of the distribution and spread of urban american visceral leishmaniasis, thus aiding in the design of regional surveillance and control strategies.
Lutzomyia longipalpis behavior and control at an urban visceral leishmaniasis focus in Argentina
Santini, Maria Soledad;Salomón, Oscar Daniel;Acardi, Soraya Alejandra;Sandoval, Enrique Adolfo;Tartaglino, Lilian;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2010, DOI: 10.1590/S0036-46652010000400004
Abstract: during the earlier stages of visceral leishmaniasis transmission in posadas city, misiones, both the night activity and attraction to humans of lutzomyia longipalpis were assessed, in order to provide preliminary recommendations. the impact of peridomestic deltamethrin spraying performed by local officials was also evaluated. although lu. longipalpis were found in traps located over a dog the entire night, 90% of the females were captured from 20.30h to 1.30h, and only landed on a human when he was at a distance of 1.5 m from the dog. peridomestic spraying of deltamethrin (25 mg/m2) reduced the sand fly capture up to seven days post-intervention without dispersion in the border of the sprayed areas. these results support the recommendations about time-space focus of the protection measures: first half of the night, in the backyard, with pets and domestic animals kept at least 5 m from humans. the deltamethrin as it was used did not seem very effective in this scenario; neither did the eventual use of bed nets, at least in adults, due to the place/hour of sand fly higher activity. this study strengthens the need for a multidisciplinary approach to develop prevention strategies based both on biological and anthropological studies.
Distribución de vectores de leishmaniasis visceral en la Provincia de Corrientes, 2008 Distribution of vectors of visceral leishmaniasis in the Province of Corrientes, 2008
Oscar Daniel Salomón,Ladys K. Ramos,María Gabriela Quintana,Soraya A. Acardi
Medicina (Buenos Aires) , 2009,
Abstract: La leishmaniasis visceral es una parasitosis de importancia en salud pública, producida por Leishmania infantum chagasi. A partir de la urbanización y emergencia en el sur de Brasil y Paraguay, se notificó la presencia del insecto vector Lutzomyia longipalpis en la Argentina; en Formosa, 2004, y en Misiones el primer caso humano en 2006. La notificación de casos de reservorios caninos infectados en la provincia de Corrientes, contigua a Misiones, determinó la búsqueda del vector. Mediante trampeos estandarizados en diciembre del 2008 se capturaron 376 Lu. longipalpis en Ituzaingó, Virasoro, Santo Tomé, Garruchos, Riachuelo, Corrientes y Monte Caseros. Se confirma el riesgo de transmisión vectorial autóctona de leishmaniasis visceral en la provincia de Corrientes. La distribución de vectores en áreas urbanas densamente pobladas, con intenso tránsito de reservorios caninos desde zonas de alta transmisión, y la presencia de reservorios infectados, implica a su vez riesgo epidémico. Visceral leishmaniasis (VL) is a relevant parasitic disease in public health, produced by Leishmania infantum chagasi. Since the urbanization and emergence in Southern Brazil and Paraguay, the vector Lutzomyia longipalpis in Formosa, 2004, and the first human visceral leishmaniasis case in Misiones, 2006, have been reported in Argentina. Due to the reports of canine VL, a search of the vector in the Province of Corrientes, contiguous to Misiones, was performed during December 2008. Standarized trapping detected 376 Lu. longipalpis in Ituzaingó, Virasoro, Santo Tomé, Garruchos, Riachuelo, Corrientes and Monte Caseros localities. The risk of autochtonous vectorial transmission was then confirmed in the Province of Corrientes. The distribution of vectors in populated urban areas, with intense transit of canine reservoirs from localities with high transmission, and the existence of infected reservoirs, also implies epidemic risk.
Distribución de vectores de leishmaniasis visceral en la Provincia de Corrientes, 2008
Salomón,Oscar Daniel; Ramos,Ladys K.; Quintana,María Gabriela; Acardi,Soraya A.; Santini,María Soledad; Schneider,Adolfo;
Medicina (Buenos Aires) , 2009,
Abstract: visceral leishmaniasis (vl) is a relevant parasitic disease in public health, produced by leishmania infantum chagasi. since the urbanization and emergence in southern brazil and paraguay, the vector lutzomyia longipalpis in formosa, 2004, and the first human visceral leishmaniasis case in misiones, 2006, have been reported in argentina. due to the reports of canine vl, a search of the vector in the province of corrientes, contiguous to misiones, was performed during december 2008. standarized trapping detected 376 lu. longipalpis in ituzaingó, virasoro, santo tomé, garruchos, riachuelo, corrientes and monte caseros localities. the risk of autochtonous vectorial transmission was then confirmed in the province of corrientes. the distribution of vectors in populated urban areas, with intense transit of canine reservoirs from localities with high transmission, and the existence of infected reservoirs, also implies epidemic risk.
Detection of Leishmania infantum in naturally infected Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae) and Canis familiaris in Misiones, Argentina: the first report of a PCR-RFLP and sequencing-based confirmation assay
Acardi, Soraya Alejandra;Liotta, Domingo Javier;Santini, María Soledad;Romagosa, Carlo Mariano;Salomón, Oscar Daniel;
Memórias do Instituto Oswaldo Cruz , 2010, DOI: 10.1590/S0074-02762010000600011
Abstract: in this study, a genotypification of leishmaniawas performed using polimerase chain reaction-restriction fragment length polymorfism (pcr-rflp) and sequencing techniques to identify species of leishmaniaparasites in phlebotomine sand flies and dogs naturally infected. between january-february of 2009, cdc light traps were used to collect insect samples from 13 capture sites in the municipality of posadas, which is located in the province of misiones of argentina. sand flies identified as lutzomyia longipalpiswere grouped into 28 separate pools for molecular biological analysis. canine samples were taken from lymph node aspirates of two symptomatic stray animals that had been positively diagnosed with canine visceral leishmaniasis. one vector pool of 10 sand flies (1 out of the 28 pools tested) and both of the canine samples tested positively for leishmania infantumby pcr and rflp analysis. pcr products were confirmed by sequencing and showed a maximum identity with l. infantum. given that infection was detected in one out of the 28 pools and that at least one infected insect was infected, it was possible to infer an infection rate at least of 0.47% for lu. longipalpisamong the analyzed samples. these results contribute to incriminate lu. longipalpis as the vector of l. infantumin the municipality of posadas, where cases of the disease in humans and dogs have been reported since 2005.
Transmisión de la Leishmaniasis tegumentaria en la Argentina
Salomón,Oscar D.; Orellano,Pablo W.; Quintana,María G.; Pérez,Sandra; Sosa Estani,Sergio; Acardi,Soraya; Lamfri,Mario;
Medicina (Buenos Aires) , 2006,
Abstract: the total cases of tegumentary leishmaniasis in argentina increased since 1985, as the frequency, intensity and geographic distribution of the epidemic outbreaks, and the relative incidence in women and children. the characterization of the new scenarios of transmission generated in the last decades is needed for the design of appropriate control measures. with this aim, four outbreaks were described as case studies, based on epidemiological and entomological data together with the qualitative analysis of satellite imaginery: urugua-í (2004), esperanza (1998), alberdi (2003) and monteros-simoca (2004). these descriptions allowed to define four scenarios of transmission according to a cycle: a) sylvatic with transmission in primary or residual vegetation; b) sylvatic with eventual peridomestic transmission due to changes within patches of residual vegetation; c) sylvatic with peridomestic transmission in domiciles contiguous with the residual vegetation; and d) peridomestic in rural, ruralized periurban or urban-rural interfase environment. all the epidemic outbreaks since 1985 were consistent with the scenarios characterized here, indicating that the tipification is comprehensive of the transmission of tegumentary leishmaniasis in argentina up to now. in conclusion, four scenarios of epidemic outbreaks were defined for argentina, while it is highlighted the qualitative analysis of field and remote sensing data as a tool to understand the epidemiology of the disease, and so to develop adequate measures for prevention and control.
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