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Search Results: 1 - 10 of 342140 matches for " Abelardo Silva Júnior "
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Porcine Circovirus 2: Immunopathogenesis and Recent Developments in Vaccines  [PDF]
Abelardo Silva Júnior, Otávio Valério de Carvalho, Luiz Henrique Silva Bulos, Juliana Lopes Rangel Fietto, Mauro Pires Moraes, Márcia Rogéria de Almeida
World Journal of Vaccines (WJV) , 2012, DOI: 10.4236/wjv.2012.22013
Abstract: Porcine circovirus 2 (PCV2) is currently considered an important etiologic agent of swine and its infection has potentially serious economic impact on the swine industry worldwide. This virus is frequently associated with postweaning multisystemic wasting syndrome (PMWS), and also with other clinical conditions such as porcine dermatitis and nephropathy syndrome (PDNS), late-term abortions, reproductive failure in sows, proliferative and necrotizing pneumonia and congenital tremors. The term porcine circovirus-associated disease (PCVAD) is currently used to refer to any of these diseases when they are associated with PCV2 infection. The PCV2 was recognized as a pathogen in 1997, and many questions regarding its biology and pathogenesis remain unanswered. Currently, some studies have shown the production of new vaccine candidates and field efficacy testing of commercial vaccines. This review discusses some major points concerned with immunopathogenesis and vaccines for PCV2 infection.
Variabilidade espacial de parametros de crescimento da mamoneira e de atributos físico-químicos em Neossolo
Silva, Júlio J. N.;Montenegro, Abelardo A. A.;Silva, ênio F. F.;Fontes Júnior, Robertson V. P.;Silva, Ana P. N.;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2010, DOI: 10.1590/S1415-43662010000900003
Abstract: the aim of this study was to evaluate the potential of the castor bean crop in a fluvisol of pernambuco "agreste". the research was conducted at the nossa senhora do rosário farm, pesqueira-pe. the brs 188 - paragua?u castor bean was cultivated, which, according to the literature, is suitable for the local conditions. the experiment was conducted under rainfed condition. classical statistics and geostatistics techniques were applied to observe the degree of dependency for the attributes and the spatial variability of soil texture, electrical conductivity of saturation extract (cees) and sodium (na) content, as well as the crop stem diameter and height of the plants. all parameters adjusted to a normal distribution, except stem diameter, at 200 days after sowing, showing a medium to low degree of spatial dependence. among the studied variables only cees showed pure nugget effect, adjusting the others to theoretical models of semivariograms. the produced contour maps allowed the observation of more heterogeneous areas, mainly in the most sandy area where better crop growth was verified and where a local management strategy must be developed.
O Paquist?o e as estratégias ocidentais para a ásia Meridional
Arantes Júnior, Abelardo;
Revista Brasileira de Política Internacional , 2003, DOI: 10.1590/S0034-73292003000100009
Abstract: faced with the risk of a nuclear conflict between pakistan and india, the western powers have tried to exert a measure of control upon events in south asia; they urged those two countries to start bilateral negotiations, promoted non-proliferation, and from time to time applied sanctions. pakistan is very sensitive to western policies, above all those of the united states, but it never gave up two fundamental criteria of its foreign policy: the demand of self-determination for kashmir and nuclear deterrence. the united states now shows unmatched power and influence, but after the iraqi crisis the unity of the great powers became shaky. at the same time the return to a civilian and parliamentarian regime in pakistan added strength to the nationalist and islamic parties, which want to contain united states influence and favour increased links with france, germany, russia, and china. in this new, fluid political environment, the evolution of indian-pakistani relations will have a strong impact on western strategies for south asia and for the world at large.
Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein
Silva Júnior, Abelardo;Castro, Luiza A.;Chiarelli Neto, Orlando;Silva, Fernanda M.F.;Vidigal, Pedro M.P.;Moraes, Mauro P.;Almeida, Márcia R.;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000100012
Abstract: porcine circovirus 2 (pcv2) is generally associated with the porcine circovirosis syndrome, which is considered an important disease of swine and has potentially serious economic impact on the swine industry worldwide. this article describes the construction of a recombinant plasmid expressing the pcv2 structural protein and the evaluation of cellular and humoral immune responses produced by this recombinant vaccine in balb/c mice. the vaccine candidate was obtained and analyzed in vivo, in an effort to determine the ability to induce a specific immune response in mice. dna was extracted from a brazilian pcv2 isolate and the gene coding for cap protein was amplified by pcr and inserted into an expression plasmid. groups of balb/c mice were inoculated intra-muscularly and intradermally in a 15-day interval, with 100 μg and 50 μg of the vaccine construct, respectively. another group was inoculated intramuscularly with 100 μg of empty plasmid, corresponding to the control group. seroconversion and cellular response in balb/c mice were compared and used for vaccine evaluation. seroconversion was analyzed by elisa. after a series of 3 immunizations the spleen cells of the immunized animals were used to perform lymphocyte proliferation assays. seroconversion to pcv2 was detected by elisa in the animals inoculated with the vaccine construct when compared with control groups. lymphocyte proliferation assays showed a stronger cell proliferation in the inoculated animals compared with the control group. thus, the vaccine candidate construct demonstrated to be able to induce both humoral and cellular responses in inoculated mice.
Estimativa da transpira??o em cafeeiros utilizando-se sensores de dissipa??o térmica
Pimentel, Jussálvia da S.;Silva, Tonny J. A.;Borges Júnior, Jo?o C. F.;Folegatti, Marcos V.;Montenegro, Abelardo A. A.;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2010, DOI: 10.1590/S1415-43662010000200010
Abstract: some methods are capable of directly determining the amount of water consumed by a plant, among them, the "heat dissipation method" or granier method. this study aimed to: build and calibrate sensors of thermal dissipation; to evaluate the transpiration as a function of the position of insertion of the sensor in the plant (north, south, east or west) and to compare the results with the meteorological elements and the evapotranspiration of reference. in 24 coffee plants, thermal dissipation sensors adapted from granier (1985) were installed, where the whole thermocouple and resistance were inserted into the needle. density of the sap flow was obtained from the variation of the difference in temperature between the probes and accompanied transpiration according to the transition between day and night periods. the experiment was evaluated during 3 periods: before flowering, during the flowering and at the beginning of fruiting. there was no significant difference between the angles of insertion to the sap flow. regarding the meteorological elements, radiation and relative humidity were the most determinant factors in transpiration, obtained by analysis of regressions with, r2 coefficients between 0.84 and 0.80.
Avalia??o da salinidade de neossolo usando-se dispositivo de indu??o eletromagnética
Montenegro, Abelardo A. A.;C. Netto, Manoel L.;Montenegro, Suzana M. G.;Silva, ênio F. F.;Fontes Júnior, Robertson V. P.;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2010, DOI: 10.1590/S1415-43662010000600007
Abstract: salt concentration in soil and in the available waters in irrigated areas in the semi-arid regions is not always suitable, and it may interfere with the agricultural yield. in this work, soil salinity has been estimated from readings of an electrical magnetic device em38 in an alluvial valley, considering the apparent soil salinity (em). the equipment has been placed not only at the soil surface, but also at different heights above the soil level, as a way to encompass distinct vertical scales on the actual soil electrical conductivity estimation (ecr). multiple regression models have been developed for the local conditions. the results have been analysed both by descriptive statistics and by the geostatistical methodology. the ecr estimates fitted to a lognormal distribution and presented a spatial dependence at a plot scale in the 0-30, 30-60 and 60-90 cm layers. it has been verified that the em38 is a suitable tool for soil salinity mapping, when local calibrations are considered, at plot scale, and in the valley scale, even in areas with relevant vertical heterogeneities. determination coefficients higher than 90% has been verified for the multiple linear regression models.
Retrospective study on Porcine circovirus-2 by nested pcr and real time pcr in archived tissues from 1978 in Brazil
Silva, Fernanda Miquelitto Figueira da;Silva Júnior, Abelardo;Peternelli, Ethel Fernandes de Oliveira;Viana, Vinícius Winter;Chiarelli Neto, Orlando;Fietto, Juliana Lopes Rangel;Vargas, Marlene Izabel;Nero, Luís Augusto;Almeida, Márcia Rogéria de;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000300039
Abstract: porcine circovirus-2 (pcv-2) infection is currently considered an important disease of swine. the pathogenic agent was first described in brazil in 2000. this study detected the pcv-2 dna in four brazilian pig tissues collected between 1978 and 1979. this observation is the oldest description of this virus in brazil.
Immunopathogenic and Neurological Mechanisms of Canine Distemper Virus
Otávio Valério Carvalho,Clarisse Vieira Botelho,Caroline Gracielle Torres Ferreira,Paulo Oldemar Scherer,Jamária Adriana Pinheiro Soares-Martins,Márcia Rogéria Almeida,Abelardo Silva Júnior
Advances in Virology , 2012, DOI: 10.1155/2012/163860
Abstract: Canine distemper is a highly contagious viral disease caused by the canine distemper virus (CDV), which is a member of the Morbillivirus genus, Paramyxoviridae family. Animals that most commonly suffer from this disease belong to the Canidae family; however, the spectrum of natural hosts for CDV also includes several other families of the order Carnivora. The infectious disease presents worldwide distribution and maintains a high incidence and high levels of lethality, despite the availability of effective vaccines, and no specific treatment. CDV infection in dogs is characterized by the presentation of systemic and/or neurological courses, and viral persistence in some organs, including the central nervous system (CNS) and lymphoid tissues. An elucidation of the pathogenic mechanisms involved in canine distemper disease will lead to a better understanding of the injuries and clinical manifestations caused by CDV. Ultimately, further insight about this disease will enable the improvement of diagnostic methods as well as therapeutic studies. 1. Introduction Canine distemper is an infectious disease caused by a member of the Morbillivirus genus, Paramyxoviridae family, infecting a broad range of terrestric and aquatic carnivores. Canine distemper virus (CDV) is an enveloped virion which contains a nonsegmented single-stranded negative-sense RNA genome that encodes six structural (nucleocapsid N, matrix M, fusion F, hemagglutinin H, phospho-P and large-L proteins) and two nonstructural (C and V proteins) proteins [1]. CDV has been reported in dogs, ferrets, wild dogs, foxes, jackals, coyotes, hyenas [2], lions, tigers, leopards, cheetahs [3], seals, sea lions, and dolphins [4]. The domestic dog is the most suffered species, and, although the disease has been also found in big cats, CDV has not been detected in domestic cats. However, experimental infection with SPF cats revealed that this species can sustain CDV replication with pronounced lymphopenia, without displaying any clinical signs [5, 6]. The Morbillivirus genus includes other important highly infectious pathogens like measles (MV) and rinderpest viruses (RPV), and almost all members present equivalent tropism and tissue distribution in their respective hosts. Morbilliviruses are transmitted by aerosols and produce clinical similarities, such as fever, serous nasal discharge, and cough, as well as respiratory and gastrointestinal signs often complicated by secondary bacterial infections. Furthermore, the most notorious property of morbillivirus infection is the establishing of severe transitory
Imunogenicidade de isolados de herpesvírus bovino 5 como candidatos à vacina
Souza, Luiz Felipe Louren?o de;Silva Júnior, Abelardo;Freitas, Carla Maria Batista de;Freitas, Ethel Cardoso de;Flores, Eduardo Furtado;Almeida, Márcia Rogéria de;Moraes, Mauro Pires;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000100026
Abstract: herpesvirus bovine 5 (bohv-5) is the agent of bovine herpetic menigoencephalitis. the neurological disease associated with the infection is highly lethal in young cattle and it is widespread in brazil. control of the clinical signs caused by herpesviruses is based mainly on the immunization of cattle. a comparative study was performed among brazilian bohv-5 isolates to select the more antigenic virus. inactivated vaccines were formulated using isolates iso9898292, sv507, sv163, 1807 and evi145 and administered to five groups of 10 sheep, each animal receiving two intramuscular doses with a 21 days interval. blood collection for serology by virusneutralization were performed until the 63rd day after the first vaccination, two peaks in the curve of antibodies were observed, the first on day 14, ranged from 23.l to 138.6. the second peak was observed 14 days after the booster, and ranged from 301.3 to 1017.5. in the 42nd day after the booster, it was observed a titer variation from 82.4 to 305.9. the differences among antibody titers of each group of suggests a lower antigenicity of isolate iso9898292 in comparison with the others, demonstrating a possible antigenic variation among the isolates. thus, all isolates, with exception of iso9898292, were immunogenic for the induction of neutralizing antibodies.
Recent changes in Clostridium difficile infection
Silva Júnior, Moacyr;
Einstein (S?o Paulo) , 2012, DOI: 10.1590/S1679-45082012000100023
Abstract: clostridium difficile is the main cause of nosocomial diarrhea. diarrhea associated with c. difficile has increased incidence, morbidity, and mortality in the last few years. the major related risk factors include use of antibiotics, elderly patients and prolonged hospital stay. many patients receive combinations of antibiotics or multiple antibiotics, which represents the main risk to develop diarrhea associated to c. difficile or its recurrence. therefore, interventions to improve antibiotic prescribing, as well as compliance with infection control measures can reduce hospital-acquired c. difficile infections. this review addresses the epidemiological changes in c. difficile disease and its treatment.
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