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Search Results: 1 - 10 of 20701 matches for " Abbas Ali Imani Fooladi "
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A method to survey heat labile anti-tuberculosis drugs
Abbas Ali Imani Fooladi,Majid Riazipour
Jundishapur Journal of Microbiology , 2009,
Abstract: Introduction and objective: The appearance of resistance to anti-tuberculosis drugs has generated research to find new and more effective drugs. L wenstein-Jensen medium (LJ) is frequently used for culturing strains of Mycobacterium tuberculosis. A group of antimicrobial substances used in treating tuberculosis is sensitive to heat and cannot be used on LJ medium. Research now aims to setup a modified method for evaluation of heat labile drugs in LS medium. Materials and methods: In this study, we investigated culturing M. tuberculosis for 48h on Middlebrook 7H9 broth medium with antituberculosis drugs and re culturing on LJ medium (without antibiotic) and incubating for 40 days.Results: Our results after 48h of contact of the strains with antibiotic were comparable with the standard method of culture on Middlebrook 7H10 agar medium containing antibiotic. Therefore, 48h is a suitable time for primary contact between mycobacterium and heat labile antibiotics. Conclusion: This modified method can be applied to LJ medium instead of expensive Middlebrook 7H10 agar medium for evaluation of heat labile anti tuberculosis drugs.
Miliary tuberculosis with empyema, a case report
Mohammad Javad Hosseini,Abbas Ali Imani Fooladi
Jundishapur Journal of Microbiology , 2010,
Abstract: We report a 39 year-old patient who presented with chest pain, malaise, lassitude, anorexia, weight loss, fever, chills, productive coughs, pleural pain in the left thorax area and septic empyema without hemoptysis. Laboratory investigations’ including tuberculosis (TB) skin test by PPD, HIV, HBV and HCV serologic tests were negative. The CBC showed anemia and leucocytosis. The ESR was elevated and he had hypoalbuminaemia. Both chest radiograph and high-resolution CT scan showed miliary infiltrates and diffused reticulonodular lung lesions. He was diagnosed with miliary tuberculosis (MTB) via direct staining, PCR and culture from open window region washing sample but not from bronco-alveolar lavage. He was treated with antituberculosis drugs.
Survey of T-2 Toxin Present in Cereals Destined for Human Consumption
Majid Riazipour,Abbas Ali Imani Fooladi,Ghasem Bagherpour
Jundishapur Journal of Microbiology , 2012,
Abstract: Background: A variety of agricultural products are exposed to fungal contamination from the early stages of planting, until their final consumption. T-2 mycotoxin is toxic to humans and to all animal species, it is mainly produced by the various Fusarium species including; F. sporotrichioides, F. poae, F equiseti, and F. acuminatum, and occasionally by other genera species, therefore, measuring T-2 toxin levels is very important in cereals.Objectives: We examined the occurrence and levels of T-2 mycotoxin in grains for human consumption.Materials and Methods: Rice, barley and wheat samples, 23, 16 and 7 respectively, were collected from the staple stores of nine food cooking centers in Tehran. After pulverizing the samples, they were extracted using a methanol-water solution (70:30), then analysed with an enzyme linked immunosorbent assay (ELISA), based on the monoclonal antibodies, the amount of T-2 mycotoxin was measured in their extracts.Results: All of the tested samples were contaminated with T-2 toxin at different levels ranging from 7.9 to 65.9 μg/kg (mean: 17.9 ± 2.1). Wheat samples had the highest level of contamination at approximately 42.4 μg/kg (± 8.4). However, both barley and rice were also affected with contamination levels of 18.3 (±2) and 12.5 (± 0.56) μg/kg respectively.Conclusions: Although the majority of samples were based on Iranian national standards, a small number of specimens (13.9 %) were contaminated at higher than acceptable limits. The extent of the impurities with T-2 toxin is an indicator of the current normal prevalence of mycotoxins in agricultural products destined for human consumption in this country, and the risk of exposure to the chronic effects of this toxin. Overall, this study showed that the level of mycotoxins in food products should be checked before they are bought or consumed..--------------------------------------------------------------------------------Implication for health policy/practice/research/medical education:Our study suggested that the levels of mycotoxins in products should be detected before buying and be discarded from human consumptive cycle if the grains are contaminated more than allowable limit..Please cite this paper as:Riazipour M, Imani Fooladi AA, Bagherpour G. Survey of T-2 Toxin Present in Cereals Destined for Human Consumption. Jundishapur J Microbiol. 2012;5(3):497-501. DOI: 10.5812/jjm.4251.
Assessment of carboxyl esterase activity in clinical isolates of Candida albicans
Majid Riazipour,Hamid Reza Tavakoli,Abbas Ali Imani Fooladi
Jundishapur Journal of Microbiology , 2011,
Abstract: Introduction and objective: Esterase activity is used in evaluation and correlation of strains in fungi. Our previous study showed that Candida albicans has a kind of intracellular esterase activity in yeast extract, peptone, and glucose medium (YPG). The aim of this research was to study the qualitative and quantitative differences of this enzymatic activity among clinical isolates of this yeast.Materials and methods: Candida albicans isolates which have been kept on Sabouraud dextrose agar medium by continuous passage were grown in YPG medium for 48h in order to induce enzymatic production. In the next step, yeast cells were collected and then broken with glass bead. Esterase activity of cytoplasmic extract of isolates was measured by colorimetric method. Besides, five synthetic substrates were used to assess the qualitative differences in this enzymatic activity.Results: The cytoplasmic extract of 12 C. albicans isolates demonstrated an esterase activity to all used substrates and no significant qualitative and quantitative differences were found in this enzymatic activity. The average enzymatic activity of all isolates had a reversed relation to the number of carbon atoms in carboxyl substrates (except for alpha-naphtyle laurate). The amount of this activity for alpha-naphtyle acetate, beta-naphtyle acetate, alpha- naphtyle caprilate, alpha- naphtyle laurate, and alpha-palmitate were 14.4, 8.45, 0.94, 0.42, 0.75 unit (μM/mg protein in min), respectively.Conclusion: The observed fluctuation in esterase activity of clinical isolates of C. albicans might be useful in tracking its sub species in epidemiological purposes.
Antimicrobial Effect of Chloroformic Garlic Extract on Mycobacterium Tuberculosis
Abbas Ali Imani-Fooladi,Morteza Sattari,Kiyumars Ghazi Saeidi
Pakistan Journal of Biological Sciences , 2006,
Abstract: This study assesses the antimicrobial effect of chloroformic extract of garlic on strains of mycobacterium tuberculosis. A standard H37RV isolate and isolates from patients with drug resistant pulmonary tuberculosis were used. A strain sensitive to 4 drugs (rifampin, isoniazid, ethambutol and streptomycin), resistant to the four drugs, resistant to two drugs and a strain resistant to one drug, were used. The antimicrobial effect was tested in vitro with minimum inhibitory concentrations of the chloroformic extract of garlic on the mycobacteria. Middel Broke 7H10 agar medium with 1:128 dilution or (167±SD μg mL-1) of chloroformic extract of garlic were used. Our study showed that garlic extract is effective in inhibiting growth of not only drug sensitive, but also drug resistant isolates of mycobacterium tuberculosis.
Smad Molecules Expression Pattern in Human Bronchial Airway Induced by Sulfur Mustard
Maryam Adelipour,Abbas Ali Imani Fooladi,Samaneh Yazdani,Ensieh Vahedi
Iranian Journal Of Allergy, Asthma and Immunology , 2011,
Abstract: Airway remodelling is characterized by the thickening and reorganization of the airways seen in mustard lung patients. Mustard lung is the general description for the chronic obstructive pulmonary disease induced by sulfur mustard(SM). Pulmonary disease was diagnosed as the most important disorder in individuals that had been exposed to sulfur mustard. Sulfur mustard is a chemical warfare agent developed during Wars. Iraqi forces frequently used it against Iranian during Iran -Iraq in the 1980-1988. Peribronchial fibrosis result from airway remodeling that include excess of collagen of extracellular matrix deposition in the airway wall. Some of Smads families in association with TGF-β are involved in airway remodeling due to lung fibrosis. In the present study we compared the mRNA expression of Smad2, Smad3, and Smad4 and Smad7 genes in airway wall biopsies of chemical-injured patients with non-injured patients as control. We used airway wall biopsies of ten unexposed patients and fifteen SM-induced patients. Smads expression was evaluated by RT-PCR followed by bands densitometry. Expression levels of Smad3 and Smad4 in SM exposed patients were upregulated but Smad2 and Smad7 was not significantly altered. Our results revealed that Smad3, and 4 may be involved in airway remodeling process in SM induced patients by activation of TGF-β. Smad pathway is the most represented signaling mechanism for airway remodeling and peribronchial fibrosis. The complex of Smads in the nucleus affects a series of genes that results in peribronchial fibrosis in SM-induced patients.
Evaluation of new primers for detecting toxigenic vibrio cholerae by multiplex PCR
Jalil F. Mehrabadi,Parisa Morsali,Hamideh Rohani nejad,Abbas Ali Imani Fooladi
Microbiology Research , 2011, DOI: 10.4081/mr.2011.e1
Abstract: Vibrio cholerae is the etiological agent of cholera that has emerged as an endemic disease in different regions of the world in recent years. Traditional microbial culture and microscopy methods are considered to be the best standard for diagnosing V. cholerae infection. These methods, however, delay any available confirmatory answer by days. Molecular methods have the potential to provide sensitive, accurate, and rapid analysis of V. cholerae infection. We have developed a multiplex PCR assay to detect virulence and toxigenic-associated (VTA) genes (ctxA, tcpA, and ompW). To evaluate PCR specificity, additional bacteria from the enterobacteriaceae family (Salmonella typhi, Shigella dysantry, and entrotoxigenic E. coli) and Aeromonas hidrophyla were examined in this study. Specificity tests were evaluated using the genome dilution method. Importantly, the results show that our PCR specificity method represents the best tool for the rapid detection of VTA genes because of its simplicity, cost effectiveness, and accuracy. This multiplex PCR method can be used for examining the existence of VTA genes in patient samples, and therefore will distinguish V. cholerae from other vibrios and bacteria. This method is able to detect 10-100 colony forming units (CFUs) of V.Cholerae and 8.5-85 picograms (pg) of genomic DNA. The multiplex PCR method is also more specific and sensitive than other methods, validating it as an appropriate and sensitive tool for detecting the presence of toxigenic and pathogenic V. cholerae.
Prevalence and Evaluation of Toxin Genes among Uropathogenic Escherichia Coli Clinical Isolates by Duplex PCR
Javad Saraylu,Jalil Fallah Mehrabadi,Abbas Ali Imani Fooladi,Aylar Sabbaghi
Journal of Medical Bacteriology , 2012,
Abstract: Background: One of the most common infections in human is urinary tract infection (UTI) and Uropathogenic Escherichia coli is one of its major causative agents. UTI is extremely common among young women. Children under age 5 are also highly at risk. Considering the prevalence of this disease, it is necessary to design an appropriate diagnostic method for its effective diagnosis. The aim of present study was to identify the prevalence of two virulence genes (sat and vat) among Uropathogenic E. coli isolates.Methods: Urine samples were taken from 350 patients with urinary tract infection. The samples were cultured on EMB agar and Blood agar. The suspected E. coli colonies were isolated and confirmed by biochemical tests. The genomic DNA was extracted from 297 isolated E. coli and target genes were amplified by PCR. The amplicons were sequenced and analyzed with ClustalW software. Moreover, data analysis was performed by using SPSS software. Subsequently, Duplex PCR was optimized for simultaneous detection of two genes.Results: The prevalence of sat and vat genes were 75 (n: 225) and 36 (n: 106) percent, respectively. In addition, less than 4% (n: 11) of clinical isolates comprised two genes.Conclusion: According to the conducted research, molecular identification of Uropathogenic E .coli strains according to detection of sat gene is potentially an appropriate method and could be noted for diagnosis.
Prevalence and Evaluation of Toxin Genes among Uropathogenic Escherichia coli Clinical Isolates by Duplex PCR
Javad Saraylu,Jalil Fallah Mehrabadi,Abbas Ali Imani Fooladi,Aylar Sabbaghi
Journal of Medical Bacteriology , 2012,
Abstract: Background: One of the most common infections in human is urinary tract infection (UTI) and Uropathogenic Escherichia coli is one of its major causative agents. UTI is extremely common among young women. Children under age 5 are also highly at risk. Considering the prevalence of this disease, it is necessary to design an appropriate diagnostic method for its effective diagnosis. The aim of present study was to identify the prevalence of two virulence genes (sat and vat) among Uropathogenic E. coli isolates. Methods: Urine samples were taken from 350 patients with urinary tract infection. The samples were cultured on EMB agar and Blood agar. The suspected E. coli colonies were isolated and confirmed by biochemical tests. The genomic DNA was extracted from 297 isolated E. coli and target genes were amplified by PCR. The amplicons were sequenced and analyzed with ClustalW software. Moreover, data analysis was performed by using SPSS software. Subsequently, Duplex PCR was optimized for simultaneous detection of two genes. Results: The prevalence of sat and vat genes were 75 (n: 225) and 36 (n: 106) percent, respectively. In addition, less than 4% (n: 11) of clinical isolates comprised two genes. Conclusion: According to the conducted research, molecular identification of Uropathogenic E .coli strains according to detection of sat gene is potentially an appropriate method and could be noted for diagnosis.
Designing and analyzing the structure of Tat-BoNT/A(1-448) fusion protein: An in silico approach
Jafar Amani, Parvaneh Saffarian, Shahin Najar-Pirayeh, Abbas Ali Imani-Fooladi
Molecular Biology Research Communications , 2014,
Abstract: Clostridium botulinum type A (BoNT/A) produces a neurotoxin recently found to be useful as an injectable drug for the treatment of abnormal muscle contractions. The catalytic domain of this toxin which is responsible for the main toxin activity is a zinc metalloprotease that inhibits the release of neurotransmitter mediators in neuromuscular junctions. A cell penetrating cationic peptide, Tat, which is a truncated N-terminal part of the Tat protein from human immunodeficiency virus, can help the toxin penetrate the skin uninvasively. This study aimed at an in silico analyses of the Tat-BoNT/A(1-448) fusion protein structure. A genomic construct was designed and optimized based on E. coli codon usage. The structure of mRNA as well as the properties of hypothetical chimeric protein was then analyzed by bioinformatic tools. Afterwards, the secondary and tertiary structures of the fusion protein were predicted by GOR4 and I-TASSER online web servers. The interaction with synaptosomal associated protein 25kDa (SNAP-25) was also analyzed as a natural substrate for the toxin. Based on the studied secondary and tertiary structures of the protein, the selected order of fusion proteins provides the natural activity of each peptide. Energy calculating data show that the acquired thermodynamic ensemble related to the mRNA structure was-1473.2 kJ/mol (-352.10 kcal/mol) and both total protein energy (Etotal) and shape related energy (Eshape) were calculated as -2294.2kJ/mol (-548.32 kcal/mol). The stability index of TAT-BoNT/A was computed to be 27.22 which has an acceptable stability as compared to that of native BoNT/A (22.39).
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