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Search Results: 1 - 10 of 1199 matches for " ANJA MERYANDINI "
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Characterization of Xylanase from Streptomyces spp. Strain C1-3
HAYATI Journal of Biosciences , 2007,
Abstract: Xylan is the major constituent of hemi cellulose. Several enzymes are needed to hydrolyse xylan completely, including xylanase. Currently, there is an increasing use of this enzyme. This study was carried out to characterize the xylanase from Streptomyces spp. strain C1-3. Results showed that the xylanase displayed its highest activity at pH 3 and 90 oC and was stable up to 10 hours at this conditions. Its activity increased after the addition of Cu2+, Fe2+, and Co2+ under concentration of 1 and 5 mM, respectively. The activity however, decreased after the addition of Mg2+, Ca2+ at 1 mM and Zn2+ at 5 mM. After a test with five kinds of xylan (i.e. from Birchwood, Beechwood, Arabinoxylan, Oat spelt and CMC), the xylanase of Streptomyces spp. C1-3 showed its preferences to Birchwood- and Arabino-xylan. The results showed that the xylanase of Streptomyces spp. C1-3 was characterized as a thermostable acid xylanase.
Yeast Isolation for Bioethanol Production
HAYATI Journal of Biosciences , 2012,
Abstract: We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.
Pemanfaatan Bakteri Selulolitik dan Xilanolitik yang Potensial untuk Dekomposisi Jerami Padi
Hasrul Satria Nur,Anja Meryandini,Hamim
Jurnal Tanah Tropika , 2009,
Abstract: There were 3 prospective isolates of cellulolytic bacteria resulted from the total of 31 isolates we found, i.e. C4-4, C5-1, and C11-1. Four combinations of bacteria including C4-4 + Xilanolytic (A), C5-1 + Xilanolytic (B), C11-1 + Xilanolytic (C), 45I-3 + 234P-16 (D), and control (E, without bacteria) were applied as inoculant of rice straw decomposition. In the incubation period the pH-H2O value of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) was relatively stable. The C/N ratio of all treatments decreased after 3 weeks of incubation. The C/N ratio value of A, B, C, D and E treatments were 22.48, 23.43, 27.49, 26.82, and 29.53 respectively. Decomposition rate all of combination treatments were faster than the control. The content of macro-micro nutrient of A, B, C, and D treatments increased in the end of measurement, while the control didn’t. The physical characteristic of substrate after incubation was better in A and B treatments that others. The result indicated that the combination of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) bacteria were the best combination for decomposition of rice straw.
Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16
Biodiversitas , 2009,
Abstract: Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90oC. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2 hours at 90oC. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1,98 mg/mL and 523 μmol/minute/mg, respectively.
Purification and Characterization of Streptomyces sp. SKK1-8 xylanase
Anja Meryandini,Yulin Lestari,Nunuk Widhyastuti
Makara Seri Sains , 2008,
Abstract: Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50oC measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml.
Activity of Proteolytic and Amylolytic Enzymes from Bacillus spp. Isolated from Shrimp Ponds
Microbiology Indonesia , 2009,
Abstract: Accumulation of feed excess in commercial shrimp ponds due to overfeeding could decrease water quality. Protein and starch are the primary components of shrimp feed. This study was conducted to characterize extracellular proteases and amylases of Bacillus spp. isolated from shrimp ponds. 72 proteolytic and amylolytic Bacillus spp. isolates were screened from shrimp ponds in Karawang, West Java. Ten isolates were selected for further characterization for their growth and ability to reduce total suspended solid generated from commercial shrimp feed. Bacillus sp. DA 5.2.3 and L5 showed excellent activity in reducing total suspended solid, by 37 and 30% respectively. Protease and a-amylase activities of Bacillus sp. DA 5.2.3 isolate were consistently higher than that of L5. Maximum total and specific protease activity of DA 5.2.3 isolate was 2.0 U mL-1 and 40.9 U mg-1 respectively, while the activities of the L5 isolate were 2.1 U mL-1 and 23.0 U mg-1 respectively. Based on its 16S rRNA gene sequences, Bacillus sp. DA 5.2.3 showed 99% similarity to Bacillus cereus XHJ-2-6. Bacillus sp. DA 5.2.3 could potentially be applied to maintain water quality by reducing total suspended solid in water columns of shrimp ponds.
Screening of Proteolytic Enzymes of Streptomyces sp. Local Strain and Their Characterization
Microbiology Indonesia , 2007,
Abstract: Protease of two Streptomyces sp. strain were chosen for characterization because of the large clear zonesurrounding the colony in nutrient agar media containing 1% (w/v) skim milk. Extracellular protease from the two isolates SLW 8-1 and 45I-3 were characterized following incubation of the isolate in Nutrient Broth media containing skim milk or chicken feather (1%). The optimum activity of the protease SLW 8-1 was at pH 9 and 80 oC, whereas that of the keratinase was at pH 6.5 and 70 °C. Protease of strain 45I-3 showed its optimum activity at pH 7.5 and 50 °C whereas the keratinase was at pH 8.5 and 80 °C.
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2001,
Abstract: Three anoxygenic photosyn thetic bacter ia, DS-1, DS-4 and Cas-13, have been exam in ated for their morphological and physiolog ical propert ies. All s trains were rod-sh ape ce lls with a swollen terminal end, Gram nega tive , motile, non-hal ophilic, non-alkal ophilic a n d non- acid ophilic , a nd capable of utilizing benzoate aerobically and photo-anaerobically. Sequ ence analysis of part of 16S rRNA genes showed that DS-1 and Cas- 13 we re cl osel y relate d to Rhodopseudomonas palustris Stra in 7 w ith a simila ri ty of 97%, where a s DS-4 ma y not be c losel y rela te d t o the former t wo str ai ns wit h a si milarit y of 78% based on t he constr ucte d phyloge nic tree . Sp ec tral anal ys is ind ica te d that the thr ee ba cter ia ha d ba cter io ch lorophyl a and norm al sp ir il loxa nthi n ser ies. Growth in med ium enriched with vitamin and supplemen ted with ben zoa te as their s ole C-s ources wa s bette r t han in me di um witho ut vita min. Benz oate deg ra dati on in me di u m with vita min was ac ce lerated. The ab ility to grow on benzoate withou t added vitam ins ind icated that the bacteria were able to s ynthesize th eir own vitamins.
Characterization of Xylanase Streptomyces spp. SKK1-8
HAYATI Journal of Biosciences , 2006,
Abstract: Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 oC. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 oC for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 oC. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan.
Isolation and identification of an agar-liquefying marine bacterium and some properties of its extracellular agarases
Biodiversitas , 2011,
Abstract: Faturrahman, Meryandini A, Junior MZ, Rusmana I (2011) Isolation and identification of an agar-liquefying marine bacterium and some properties of its extracellular agarases. Biodiversitas 12: 192-197. A new agar-liquefying bacterium, designated Alg3.1, was isolated from Gracilaria samples collected from the Kuta Coast at Central Lombok in West Nusa Tenggara and was identified as Aeromonas sp. on the basis of morphology, biochemical-physiological character and 16S rDNA gene sequencing. The bacterium appeared capable of liquifying agar in nutrient agar-plate within 48 hours of incubation and the agar was completely liquefied after l5 days at 29oC. When the isolate was grown in basal salts solution medium B supplemented with peptone and yeast extract, produced extracellular agarases within a short period of time (4-16 h) and the maximum agarase activity was 0.489 nkat/mL at 36h after incubation.
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