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OALib Journal期刊

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Antibacterial Quantification from Lactic Acid Bacteria Isolated from Food Sources and Soil
Akharaiyi Fred Coolborn
Journal of Food Technology , 2013,
Abstract: Indicator bacteria of pathogenic and food spoilage capabilities were isolated from food sources, water and human feaces using MacConkey agar, Deoxycholate citrate agar and selenite F medium. Lactic Acid Bacteria (LAB) were isolated from soil, wine local cheese and corn slurry using MRS medium. Well-in-agar and paper disc methods were emplored for the antagonistic assay. The sensitivity of the indicator bacteria to LAB was indicated by clear zones of inhibition. Lactobacillus acidophilus exhibited the greatest inhibitory effect while Lactobacillus jugurti and Lactobacillus casei could not demonstrate visible inhibitory affinity on all the indicator bacteria. However, the LAB antagonistic activity over the indicator bacteria, displayed more elaborate inhibitory zones in the well -in-agar (5-6 mm) than the paper disc method (3-20 mm). Also the human enteropathogen assay with well-in-agar was between (2-26 mm) over (1- 8 mm) with the paper disc method.
Water Quality Assessment of the Owena Multi-Purpose Dam, Ondo State, Southwestern Nigeria  [PDF]
Oyhakilome Gloria Irenosen, Aiyesanmi Ademola Festus, Akharaiyi Fred Coolborn
Journal of Environmental Protection (JEP) , 2012, DOI: 10.4236/jep.2012.31003
Abstract: Composite water samples taken from Owena Multi-purpose Dam in six sampling campaigns covering the wet and dry seasons were analyzed for physico-chemical and microbial characteristics using standard methods for the examination of water and wastewater jointly published by the American Public Health Association, American Water Works Association and Water Pollution Control Federation. Results showed significant (p < 0.05) seasonal variations in most measured parameters with few showing significant spatial variation. The characteristics of the water from the dam lake revealed an acceptable quality for most measured parameters with low chemical pollutants burden when compared with drinking water standards and water quality for aquaculture. However, high values of turbidity, colour, iron, manganese and microbial load were recorded compared with drinking water standards, which call for proper treatment of the water before distribution for public consumption.
Mixture of honey and ginger extract for antibacterial assessment on some clinical isolates.
OMOYA, F. O,AKHARAIYI, F. C.
International Journal of Pharmaceutical and Biological Research , 2011,
Abstract: The antibacterial activity of honey, methanol and ethanol extracts of ginger (Zingiber officinale) were investigated against some selected bacteria using the agar diffusion technique. Two Gram positive and four Gram negative bacteria were assessed for possible inhibition by the extract samples. The inhibitory potency of the extracts on the test organisms varied in the halos as inhibition effects. Though all the test organisms were susceptible to the antibacterial samples with inhibition measure between 6-3mm, E. coli was the most inhibited where an inhibitory measure of 20mm was recorded with honey, 18mm with ginger ethanol extract and 32mm with the mixture of honey and ginger ethanol extract. The pasture honey, the ethanol and methanol extracts of ginger were both positive for saponin and cardiac glycosides among the phytochemicals identified. While some of the commercial antibiotics (positive control) were not effective on the test organisms, gentamycin and streptomycin were effective with inhibitory halos ranging between 8-25mm. However, the antibacterial test samples were higher in inhibition values than the reference drugs (positive control).
Assessment of Wrap Sizes as it Affects Storage of Fufu, a Traditional Cassava Based Fermented Products
F.C. Akharaiyi,R.A.O. Gabriel
American Journal of Food Technology , 2007,
Abstract: Fufu, a traditional Cassava based fermented food product was prepared in the Laboratory and used for the study. Due to the short time storage and transportation of this particular food from one place to another on a popular demand because of its nutritional values, three wrap sizes were adopted to assess storage improvement. In this method, 50 g, 100 and 200 g of raw fufu were wrapped before cooking for the normal 1 h. The batches were stored on a wooden tray at ambient temperature in the laboratory. Quantitative determination of microbial and chemical changes occurring in the fufu samples was studied for four weeks. Microbial counts was higher in the 200 g wrap at fourth week of storage being 8.40x104 cfu (g-1) for bacteria and 2.32x103 spore (g-1) for fungi while it was 1.35x104 cfu (g-1); 1.06x103, 1.66x103 spore (g-1) bacterial and fungal counts, respectively for 50 g and 100 g wraps. Also at fourth week of storage pH was 3.80, 4.06 and 6.15; TTA was 0.45, 1.85 and 2.56% (W/W lactic acid) while moisture was 45.10, 58.40 and 70.30%, respectively for 50, 100 and 200 g wraps. In the pounded fufu at fourth week storage, colour odor, aroma and texture rating were significantly higher (p<0.05) for the 50 and 100 g wraps for overall acceptability while the 200 g wrap characters were very low thus unacceptable. This implies that smaller wrap sizes will store longer than big wrap sizes.
ANTIBACTERIAL, PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF CNESTIS FERRUGINEA DC (CONNARACEAE) EXTRACTS
Fred Coolborn Akharaiyi,Bolatito Boboye,Adetuyi,F.C.
Journal of Microbiology, Biotechnology and Food Sciences , 2012,
Abstract: To evaluate the health relevance of decoctions, infusions and concoction of medicinal plants in traditional medicine, antibacterial efficacy of crude aqueous, ethanol and petroleum ether extracts of Cnestis ferruginea leaf, stem bark and roots were assayed against nine clinical bacterial isolates namely Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus cereus, Shigalla dysenteriae, Campylobacter jejunum, a -hemolytic group A streptococcus, and Salmonella enterica ser.Typhi by agar well diffusion method. The leaf extracts had higher inhibitory effects at various degrees than the stem bark and root extracts. Chemical methods were used to determine the quality and quantity of phytochemical components where alkaloids, flavonoids, saponin, tannins, phenol and cardinolides were found present. The antioxidant activities of the extracts of the plant tested were determined by a spectrophotometric method using the stable free radical DPPH (2, 2-diphenyl-1-picrylhydrazyl). Considerable antioxidant activities were found in the plant extracts. C. ferruginea contains bioactive principles necessary for bacteria inhibition and therefore, the powder could be used as preservative, beverage and source of novel drug(s).
3. Antibacterial effect of Terminalia catappa on some selected pathogenic bacteria
F.C. Akharaiyi,R.M. Ilori,J.A. Adesida
International Journal of Pharmaceutical and Biomedical Research (IJPBR) , 2011,
Abstract: Pathogenic organisms are responsible for the deaths of many people living in both rural and urban areas. However, series of commercial antibiotics have proved ineffective in cure of many of these diseases. Curing of disease(s) is important in health care system and trial of alternative medicine is important in this for good health care delivery. Water extract of the leaf of Terminalia catappa at different stages (young, matured and old red pigmented) were studied on some selected pathogenic bacteria species In-Vitro. The leaf extracts at their different stages of maturity, exhibited therapeutic effect on the test organisms. However, the old (red pigmented) leaf extract (C) had inhibitory affinity on the tested organisms with halo ranging between 5mm-10mm in diameter, different from the matured leaf extract (B) that ranged in inhibitory halo of between 10.6mm-18.5 mm. The young leaf extract (A) of Terminalia catappa indeed showed the highest inhibitory effect on the test organisms with halos between 10.2- 20.6 mm. Bacillus cereus and Shigella dysenteriae were most inhibited with this extract (20.6 mm) while Escherichia coli was the least inhibited (10.2 mm). MIC was effective by the young leaf extract at 100-145mg/mL, the matured leaf at 130-145mg/mL and the old red leaf extract at 130-350mg/mL on the test organisms. The MBC of the young leaf extract was effective at 100-145mg/mL, the matured leaf extract at 130-160mg/mL and the old red leaf extract at 145-300mg/mL on the test organisms. The antibacterial potency of Terminalia catappa is determined so that nutritional and medicinal properties could be exploited judiciously. The results confirm the effective use of this plant in medicine, food system and pharmacy.
Growth Responses of Some Bacterial Isolates to Some Environmental Parameters  [PDF]
Esther Aanuoluwa Ekundayo, Olayinka Temitayo Ogunmefun, Ijeoma Nwaefere Oguike, Fred Coolborn Akharaiyi, Oluwakemi Sola Asoso
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.911039
Abstract: This study was carried out to investigate the antimicrobial activities of bacterial isolates of maize against plant pathogens as well as their growth responses to some environmental parameters. Twenty four bacterial isolates were obtained from maize plants collected from the Department of Biological Sciences, AfeBabalola University, Ado-Ekiti. The isolates were characterized by their biochemical and physiological characteristics and were identified as Kurthiazopfu, Morganellamorganic, Rhodococcusequi, Bacillus subtilis, Catabaterhongkongensis, Brevibacteriumotitidis, Lactobacillus coleohominis, Staphylococcus aureus, Propionibacterium acnes among others. Their responses to different NaCl concentrations, sugars, temperature as well as antibiotics were determined. Most of the isolates were able to withstand various environmental parameters in which they were subjected to. Also, eight isolates were able to ferment sucrose. The bacterial isolates showed a degree of resistance to the antibiotics tested. There was a high prevalence of multidrug resistant bacteria showing resistance to 3 - 8 drugs. The antagonistic effect of the bacterial isolates against selected fungi was determined. None of the isolates showed antagonistic potential against the fungal pathogens. However, the supposed antagonistic bacterial species can be genetically modified to produce secondary metabolites that will result in biocontrol.
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