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Search Results: 1 - 10 of 462261 matches for " A. Taghavi Moghadam "
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Identification and Purification of the Three Toxins from Odotobuthus bidentatus, Lourenco and Pezier 2002, Venom (Scorpions, Buthidae)
B. Masihipour,A.M. Zare,S.H. Navidpour,A. Taghavi Moghadam
Journal of Biological Sciences , 2009,
Abstract: Odonthobuthus belongs to Buthidae family from which 2 species O.doriea and O. bidentatus have been collected and reported from Iran. This study investigated the venom of O. bidentatus to identify mammal toxic fractions. After collecting scorpions, they were milked with electroshock technique and then the venoms was lyophilized. The LD50 of the venom was determined by injecting the venom to 18-20 g mice via., tail vein. In order to separate different fractions, the freeze dried venom was solubilized in distilled water and centrifuged at 15000 rpm for 20 min, to separate insoluble material and the clear supernatant containing soluble venom was loaded on a chromatography column packed with sephadex G50 gel and the fractions were collected according to UV absorption at 280 nm wavelength. Second fraction was toxic in Lab animal and in order to study sub fractions, fraction II (O2) was loaded first on an anionic ion exchange resin (DEAE sephacel) out of which we obtained 2 toxic fractions (O21 and O23). Then these 2 fraction were loaded on a cationic resin (CM sephadex C25) out of which, from fraction 21, we got 2 toxic fractions (O211, O213) and from fraction O23 we got one toxic fraction (O232). SDS PAGE electrophoretic studies on these toxins showed single band appearance that indicates purified toxins with molecular weight of O211 9.2, O213 8.6 and O233 6.8 kD.
Application of fermentor technology in production of diphtheria toxin
Ahmad Taghavi Moghadam,Koroush Afsharpad
Jundishapur Journal of Microbiology , 2008,
Abstract: The present study shows the production of diphtheria toxoid in fermentor as compared to old static method of production. With using 45l glass fermentor which has the provision for growth of the organisms under controlled conditions with regard to temperature, pH, aeration and addition of maltose which can be automatically monitored and maintain microorganism growth toxin production and recording all of the conditions during growth. We succeeded to produce diphtheria toxin with 115 Lf (Rammon flucolation titer) within 40h time period in a minimum possible space, then the toxin was detoxified with formaldehyde, concentrated and purified and finally quality controlled and compared with toxin produced with static method. Fermentor production showed a remarkable increase in volume, titer and immunogenecity in a shorter period of time, lower contamination and minimum labor work and requirements. Therefore this technique can be industrially used for production of diphtheria Toxin (DT) and Diphtheria Pertussis Toxin (DPT) vaccines.
Sequence Analysis of Txk from the Scorpion Mesobuthus eupeus Venom Glands Using Semi-Nested RT-PCR
Ghafar Eskandari,Abbas Jolodar,Ahmad Taghavi Moghadam
Research Journal of Biological Sciences , 2012, DOI: 10.3923/rjbsci.2011.12.14
Abstract: Natural toxins are useful probes for evaluating the involvement of K+ channels in cell activity and for investigating K+ channel structure and localization. In recent years, peptide toxins that block various K+ channels with high affinity have been purified from diverse animal venoms. One polypeptide beta neurotoxin named Txk was isolated from the venom of scorpion Mesobuthus eupeus of Khuzestan. This toxin consists of 91 amino acid residues which modulate voltage-gated sodium channels gating. In this study, cDNA of Txk β-toxin was amplified and sequence of beta neurotoxin compared with M. martensii occitanus Israelis and Tityus costatus however, the comparison suggests that the length of the peptide is close to the long-chain potassium ion channel blocker peptide family.
Survey of Shareholders Composition's Effect on Amount of Cash Dividend in Tehran Stock Exchange
Amin Taghavi Baghan,Hossein Shoghi,Abolfazl Ghadiri Moghadam,Marzieh Yazdi
Asian Journal of Finance & Accounting , 2012, DOI: 10.5296/ajfa.v4i2.2155
Abstract: In the capital market many research have been done to determine relationship between different financial variables and corporate dividend policy, but has been less attention to the ownership structure in the Iran as one of the influencing factors on dividend policy. The present study is investigate the shareholders composition effect on amount of cash dividend in Tehran Stock Exchange listed companies with exerting control free per share cash flow and the size of company in a 5-year period (2006-2010). In this regard, proposed four hypotheses. In sampling method (systematic elimination) 146 firms were selected as sample. In order to test the hypothesis were used the correlation and regression analysis. The results indicated existence of meaningful relationship between 5 percent ownership among largest shareholders, legal and real shareholders and percentage ownership of the board members with cash dividends in Tehran Stock Exchange listed companies.
The Effect of Different Levels of Dietary Phosphorus (Inorganic Phosphorus) on Performance in Broiler Chicks
A. Nouri Moghadam
International Journal of Poultry Science , 2006,
Abstract: This experiment was conducted to study the effects of available phosphorus on the growth performance, phosphorus and calcium metabolism of broiler chicks. Four hundred and five 7 days old age chicks were fed with a diet containing 0.20, 0.25, 0.30% available phosphorus (0.30, 0.35 and 0.40 % respectively total phosphorus) in a completely randomized design, consisting of 45 chicks per replicate during a 14 days experimental period (from 8 to 21 days of age). All feeding programs were isocaloric and isoprotein. chicks were put at random into 3 treatment groups (3 replicates and 135 chicks per treatment).The effects of different levels of available phosphorus on body weight, daily feed consumption, feed conversion efficiency, feed/gain ratio, weight percentage of body parts, percentage of bone ash, calcium and phosphorus percentages in bone ash, and plasma inorganic phosphorus and calcium were assessed and determined. The results showed that the effects of different levels of available phosphorus on body weight, daily feed consumption, bone ash percentage, plasma calcium and phosphorus percentages (p< 0.01) and bone ash phosphorus percentage (p< 0.05) were significant while no significant effects were observed in the case of feed conversion efficiency, feed/gain ratio, and plasma calcium. From the results obtained, it seems that increasing levels of inorganic phosphorus (non-phytate phosphorus) led to more efficiency in the broiler performance, calcium and phosphorus utilized by poultry.
Functional Motions of Candida antarctica Lipase B: A Survey through Open-Close Conformations
Mohamad Reza Ganjalikhany, Bijan Ranjbar, Amir Hossein Taghavi, Tahereh Tohidi Moghadam
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040327
Abstract: Candida antarctica lipase B (CALB) belongs to psychrophilic lipases which hydrolyze carboxyl ester bonds at low temperatures. There have been some features reported about cold-activity of the enzyme through experimental methods, whereas there is no detailed information on its mechanism of action at molecular level. Herein, a comparative molecular dynamics simulation and essential dynamics analysis have been carried out at three temperatures (5, 35 and 50°C) to trace the dominant factors in the psychrophilic properties of CALB under cold condition. The results clearly describe the effect of temperature on CALB with meaningful differences in the flexibility of the lid region (α5 helix), covering residues 141–147. Open- closed conformations have been obtained from different sets of long-term simulations (60 ns) at 5°C gave two reproducible distinct forms of CALB. The starting open conformation became closed immediately at 35 and 50°C during 60 ns of simulation, while a sequential open-closed form was observed at 5°C. These structural alterations were resulted from α5 helical movements, where the closed conformation of active site cleft was formed by displacement of both helix and its side chains. Analysis of normal mode showed concerted motions that are involved in the movement of both α5 and α10 helices. It is suggested that the functional motions needed for lypolytic activity of CALB is constructed from short-range movement of α5, accompanied by long-range movement of the domains connected to the lid region.
Delaying Apricot (cv. Shahroudi) Flower Induction by Growth Regulators Application
E. Ganji Moghadam,A. Mokhtarian
Journal of Applied Sciences , 2006,
Abstract: Control flowering time in apricot trees is very important where spring frost damage is of high risk. Application of plant growth regulators during autumn has been suggested for controlling bloom date. This experiment was conducted during 2000-2001 to examine the effects of application of different concentrations of ethephon and gibberellic acid (0, 50, 100, 200 and 300 mg L-1) at the end of August, September and October) to delay flowering in apricot Shahroudi cultivar. A factorial experiment was laid out in Completely Randomized Block Design with 3 replications. Compared to the control results showed, 100 mg L-1 of ethephon applied at the end of October delayed full bloom approximately 3 days (2000) and 7 days (2001). Gibberellic acid applied at 300 mg L-1 at the end of August delayed full bloom about 5 days (2000) and at the end of September, l0 days (2001). During both year`s gibberellic acid and ethephon applied at the higher concentrations resulted lower percentages of flower opening and fruit set.
Genetic Variation of Mahaleb (Prunus mahaleb L.) on Some Iranian Populations Using Morphological Characters
E. Ganji Moghadam,A. Khalighi
Journal of Applied Sciences , 2006,
Abstract: Prunus mahaleb L. and Prunus avium L. are the principal rootstocks used in Iran and world wide for sweet and sour cherries. This study was conducted at Khorasan Natural Resources and Agricultural Research Center with the main purpose of investigation of genetic variation and identification of mahaleb dwarf genotypes for cherry rootstock breeding. We evaluated morphological characters of 17 mahaleb populations. One-way analysis of variance was performed for determination of different regions genetic diversity, which indicated significant differences for most traits. Correlation coefficient showed significant correlation between tree vigor, crown width and crown volume and size index. Factor analysis detected that in the first factor, crown height, size index, crown width, trunk circumference and crown volume had highest factor loading, bark to wood ratio, internode and chlorophyll content were the second most important and third factor was leaf area. These factors contained 57.9% of total variance. Results also showed that mahaleb seedlings had vigor variation.
Lagrangian method for solving Lane-Emden type equation arising in astrophysics on semi-infinite domains
K. Parand,A. R. Rezaei,A. Taghavi
Physics , 2010, DOI: 10.1016/j.actaastro.2010.05.015
Abstract: In this paper we propose a Lagrangian method for solving Lane-Emden equation which is a nonlinear ordinary differential equation on semi-infinite interval. This approach is based on a Modified generalized Laguerre functions Lagrangian method. The method reduces the solution of this problem to the solution of a system of algebraic equations. We also present the comparison of this work with some well-known results and show that the present solution is acceptable.
Numerical approximations for population growth model by Rational Chebyshev and Hermite Functions collocation approach: A comparison
K. Parand,A. R. Rezaei,A. Taghavi
Mathematics , 2010, DOI: 10.1002/mma.1318
Abstract: This paper aims to compare rational Chebyshev (RC) and Hermite functions (HF) collocation approach to solve the Volterra's model for population growth of a species within a closed system. This model is a nonlinear integro-differential equation where the integral term represents the effect of toxin. This approach is based on orthogonal functions which will be defined. The collocation method reduces the solution of this problem to the solution of a system of algebraic equations. We also compare these methods with some other numerical results and show that the present approach is applicable for solving nonlinear integro-differential equations.
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