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Search Results: 1 - 10 of 461860 matches for " A. Farajnia "
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Soil Salinity and Alkalinity Map Preparation Based on Spatial Analysis of GIS (Case Study: Tabriz Plain)  [PDF]
A. Farajnia, J. Yarahmadi
Open Journal of Geology (OJG) , 2017, DOI: 10.4236/ojg.2017.76052
Abstract:

Better management of agricultural fields is related to valuable information which can derived from soil salinity and alkalinity maps. These maps are considered as one of the most important factors which restrict plant growth as well as decline crops yield. The objective of this research was preparing of soil salinity and alkalinity maps in Tabriz plain over 50,000 hectares based on different techniques of spatial analysis in GIS software. For this mean, study area was divided in 1500 × 1500 m2 grid cells. Then, geographical coordinate of each grid recorded in UTM system. So, they were transferred into GPS for navigating to the exact excavation location. After soil sampling and transferring to the lab, their EC and PH were measured in saturation extract of soil samples. So, spatial distribution of soil sampling points was prepared in form of point map by GIS software. Generalization of point information to surface was performed using different interpolation algorithms and based on standards of Soil and Water Research Institute. Accuracy of interpolated maps was evaluated due to the MAE and MBE values. The results showed that the lowest observed error is related to the Spline method and therefore, this method was used for spatial modeling of salinity and alkalinity maps in the intended area. The research findings demonstrated that from total of 50,000 hectares, only 3066 hectares were without salinity and alkalinity limitation (6.1%), 9066 hectares had low salinity and alkalinity (18.1%); 17,772 hectares had average limitation for salinity and alkalinity (35.6%) and the remaining 20,096 hectares had high and very high limitation for salinity and alkalinity.

Determination of cephalosporin acylase activity by biological and colorimetric method in bacteria
A Tanomand, R Abeshov, S Farajnia
African Journal of Biotechnology , 2009,
Abstract: The effective production of 7-aminocephalosporanic acid (7-ACA) is a matter of concern in the pharmaceutical industry because it is a starting material for the synthesis of semi synthetic cephalosporin. Therefore screening for new source of cephalosporin acylase positive bacteria is very important. The cephalosporin acylase can be found in several Pseudomonas sp. and other bacteria. To facilitate the attempts of obtaining the microorganisms with higher cephalosporin acylase activity from natural environments, development of new and specific methods for screening environmental microorganisms with cephalosporin acylase activity is very important. In this study, a biological and colorimetric method was evaluated for determination of cephalosporin acylase product in bacteria. Samples were cultured in general and selective media, and the routine biochemical laboratory tests were used for diagnosis of Pseudomonas sp. All of the isolated strains were tested for cephalosporin acylase by a biological and colorimetric method. A total of 180 Pseudomonas sp. out of 350 samples were isolated. Two strains capable of producing cephalosporin acylase were identified from 180 candidates. The Pseudomonas bacteria isolated in this study is a source for cloning and cephalosporin acylase enzyme production.
Early age onset familial Mediterranean fever associated with compound heterozygote M680I /M694V mutation
S Farajnia, A Nakhlband, M Rafeey, K Sakha
African Journal of Biotechnology , 2006,
Abstract: Familial Mediterranean fever (FMF) is an autosomal recessive genetic disorder characterized by acute episodes of fever accompanied by severe abdominal pain, pleurisy, arthritis, and skin rash. The clinical variability of the disease has been mainly attributed to MEFV gene allelic heterogeneity and partly to the influence of additional genetic and/or environmental factors. We present a 6-month-old boy who suffered from recurrent fever accompanied by abdominal pain and skin rashes. Molecular screening by polymerase chain reaction (PCR) and sequencing for common mutations causing FMF revealed presence of a 694V/680I compound heterozygote mutation in exon 10 of the related gene. This is the first report of early onset and severe phenotype FMF case associated with a 694V/680I compound heterozygote mutation.
In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts
R Pourabbas, S Farajnia, S Kimya, L Mohammadnejad, A Johnson, T Nejatian
African Journal of Biotechnology , 2009,
Abstract: Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cell attachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resin ionomer (RI) restorative material. Discs (6×2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48 and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the unincubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate was higher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures.
Short Communication Molecular cloning and sequencing of penicillin G acylase from Shigella boydii
MS Hassan, S Farajnia, R Aboshof
African Journal of Biotechnology , 2009,
Abstract: In this study, 290 non-Escherichia coli Enterobacteriasea that were isolated from environmental and clinical specimen, were sent to the laboratory for examination with routine microbiological tests for identification of isolates. After identification, non-E. coli isolates were inspected by PCR for existence of penicillin G acylase (PGA) gene. Then, a PGA positive strain (Shigella boydii) from clinical specimens was selected for further analysis. First, DNA was isolated and PCR reactions were conducted using primers based on conserved region of PGA genes. The PCR reaction resulted in amplification of a specific product with expected length. The PCR product was cloned in pGEM-T Easy vector. Sequencing revealed that the gene, composed of encodes a polypeptide of 846 amino acid residues. Analysis of obtained sequence against databases showed the highest homology (about 96%) with the PGA gene reported from S. boydii.
Screening of Pseudomonas sp. for Cephalosporin Acylase Activity
Asghar Tanomand,Rahib Abeshov,Safar Farajnia
Research Journal of Biological Sciences , 2012,
Abstract: Medically useful semisynthetic cephalosporin antibiotics are made from precursor 7-aminocepha-losporanic acid (7-ACA). Cephalosporin Acylase (CA), which catalyzes hydrolysis of both glutary l-7-aminocephalosporanic acid (GL-7ACA) and cephalosporin C (CPC) to 7-ACA, is thus a very important enzyme for producing semisynthetic beta-lactam antibiotics. The cephalosporin acylase can be found in several Pseudomonas sp. (such as P. putida, P. cepacia BY 21 P. nitroreducens, P. syringae, p. SE83, p. V22, p. SY-77, p. sp.130 , ) and other bacteria. Therefore, screening of cephalosporin asylase positive pseudomonas is very important. We isolated 130 Pseudomonas sp. from clinical and environmental specimens (75 clinical samples, 40 hospital environment samples, 10 water samples, 5 soil samples). All of isolated Pseudomonas sp. were tested for cephalosporin acylase by P.C.R method and analysis of gel electrophoresis patterns. We found only 2 cephalosporin acylase pseudomonas positive from clinical samples. These strains were selected for sequencing and cloning in E. coli and assessment of gene expression and cephalosporin acylase production in others stages of our study.
Dose-Dependent Effect of Flouxetine on 6-OHDA-Induced Catalepsy in Male Rats: A Possible Involvement of 5-HT1A Receptors
Hamdolah Sharifi,Alireza Mohajjel Nayebi,Safar Farajnia
Advanced Pharmaceutical Bulletin , 2013, DOI: 10.5681/apb.2013.033
Abstract: Purpose: Progressive loss of dopaminergic neurons of the substantia nigra pars compacta (SNc) in Parkinson’s disease (PD) leads to impairment of motor skills. Several evidences show that the role of serotonergic system in regulation of normal movement is pivotal and mediates via 5-HT1A receptors. Our previous study has shown that fluoxetine in acute injections able to attenuate catalepsy in 6-hydroxydopamine (6-OHDA)-lesioned rats. Since drugs are used chronically in clinic, in this study we attempted to evaluate effect of chronic administration of fluoxetine on 6-OHDA-induced catalepsy. Methods: Catalepsy was induced by unilateral infusion of 6-OHDA (8 μg/2 μl/rat) into the central region of SNc and assayed by using bar-test. Fluoxetine (1, 2.5, 5 and 10 mg/kg) was injected intraperitonealy (ip) for 10 days and its anti-cataleptic effect was assessed at the 10th day. Results: Fluoxetine in high doses (5 and 10 mg/kg) worsened 6-OHDA-induced catalepsy while it had anti-cataleptic effect at the dose of 1mg/kg. The anti-cataleptic effect of fluoxetine (1mg/kg) was reversed by co-administration with NAN-190 (0.5 mg/kg, ip), as a5-HT1Areceptor antagonist. Conclusion: According to the results it can be concluded that fluoxetine has anti-cataleptic effect in parkinsonian rats only at low doses, whereas at higher doses it worsens catalepsy. It’s anti-cataleptic effect is exerted through affecting on 5-HT1Areceptors. However, at high doses other mechanisms may be involved. Further clinical studies are needed to prove it’s possible clinical application as an adjuvant therapy in reducing catalepsy of PD.
The Effect of Chronic Administration of Buspirone on 6-Hydroxydopamine-Induced Catalepsy in Rats
Hamdolah Sharifi,Alireza Mohajjel Nayebi,Safar Farajnia
Advanced Pharmaceutical Bulletin , 2012,
Abstract: Purpose: Several evidences show that serotonergic neurons play a role in the regulation of movements executed by the basal ganglia. Recently we have reported that single dose of buspirone improved 6-hydroxydopamine (6-OHDA) and haloperidol-induced catalepsy. This study is aimed to investigate effect of chronic intraperitoneal (i.p.) administration of buspirone on 6-OHDA-induced catalepsy in male Wistar rats. Methods: Catalepsy was induced by unilateral infusion of 6-OHDA (8 μg/2 μl/rat) into the central region of the SNc and was assayed by the bar-test method 5, 60, 120 and 180 min after drugs administration in 10th day. The effect of buspirone (0.5, 1 and 2 mg/kg, i.p. for 10 days) was assessed in 6-OHDA-lesioned rats. Results: The results showed that chronic injection of buspirone (0.5, 1 and 2 mg/kg, i.p. for 10 days) decreased catalepsy when compared with the control group. The best anticataleptic effect was observed at the dose of 1 mg/kg. The catalepsy-improving effect of buspirone was reversed by 1-(2-methoxyphenyl)- 4-[4-(2-phthalimido) butyl]piperazine hydrobromide (NAN-190), 0.5 mg/kg, i.p.,as a 5-HT1A receptor antagonist. Conclusion: Our study indicates that chronic administration of buspirone improves catalepsy in a 6-OHDA-induced animal model of parkinson's disease (PD). We also suggest that buspirone may be used as an adjuvant therapy to increase effectiveness of antiparkinsonian drugs. In order to prove this hypothesis, further clinical studies should be done.
Screening of Pseudomonas sp. for Cephalosporin Acylase Activity
Asghar Tanomand,Rahib Abeshov,Safar Farajnia
Research Journal of Biological Sciences , 2008,
Abstract: Medically useful semisynthetic cephalosporin antibiotics are made from precursor 7-aminocepha-losporanic acid (7-ACA). Cephalosporin Acylase (CA), which catalyzes hydrolysis of both glutary l-7-aminocephalosporanic acid (GL-7ACA) and cephalosporin C (CPC) to 7-ACA, is thus a very important enzyme for producing semisynthetic beta-lactam antibiotics. The cephalosporin acylase can be found in several Pseudomonas sp. (such as P. putida, P. cepacia BY 21 P. nitroreducens, P. syringae, p. SE83, p. V22, p. SY-77, p. sp.130 ,...) and other bacteria. Therefore, screening of cephalosporin asylase positive pseudomonas is very important. We isolated 130 Pseudomonas sp. from clinical and environmental specimens (75 clinical samples, 40 hospital environment samples, 10 water samples, 5 soil samples). All of isolated Pseudomonas sp. were tested for cephalosporin acylase by P.C.R method and analysis of gel electrophoresis patterns. We found only 2 cephalosporin acylase pseudomonas positive from clinical samples. These strains were selected for sequencing and cloning in E. coli and assessment of gene expression and cephalosporin acylase production in others stages of our study.
Production and Purification of Polyclonal Antibody Against Human Kappa Light Chain
J. Abdolalizadeh,J. Majidi,S. Farajnia
Journal of Biological Sciences , 2008,
Abstract: The aim of this study was production, purification and HRP conjugation of polyclonal IgG against human kappa light chains. Three 6-month-old New Zealand White rabbits were immunized by human kappa light chain. The IgG fraction was purified by ion-exchange chromatography and labeled with Horse Radish Peroxidase (HRP). Direct ELISA was used to determine the optimum titer of HRP conjugated IgG. The purity of various IgG preparations was about 98%. The optimum dilution of prepared HRP conjugated IgG was 1:16000. The produced antibody has many application in research, clinic and education. This polyclonal antibody can be used for diagnosis and monitoring of free light chain producing diseases.
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