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Search Results: 1 - 10 of 462794 matches for " A Miramin Mohammadi "
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Flow Cytometric Analysis of Leishmania Reactive CD4+/CD8+ Lymphocyte Proliferation in Cutaneous Leishmaniasis
M Nateghi Rostami,A Khamesipour,SE Eskandari,A Miramin Mohammadi
Iranian Journal of Parasitology , 2008,
Abstract: Background: Determination of the division history of T cells in vitro is helpful in the study of effector mechanisms against infections. Technique described here uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor the proliferation. Methods: In a cross sectional study, blood samples were collected from 7 volunteers with history of cutaneous leishmania-sis (CL) and one healthy control from endemic areas in Isfahan province who referred to the Center for Research and Training in Skin Diseases and Leprosy (CRTSDL), then CD4+/CD8+ lymphocytes and CD14+ monocytes were isolated from peri-pheral blood mononuclear cells (PBMC) using mAbs and magnetic nanoparticles. CFSE labeled CD4+ or CD8+ lympho-cytes cultured with autologous monocytes in the presence of PHA, SLA, live Leishmania major or as control with-out sti-mulation. Cells were harvested after 7 days and were analyzed using flow cytometry. Results: Five consecutive divisions were monitored separately. Stimulation of CD4+ or CD8+ lymphocytes from CL sub-jects with SLA showed a significant difference in proliferation comparing with unstimulated cells (P< 0.05). The signifi-cant difference in the percentages of CD4+ cells stimulated with SLA was revealed at different divisions for each subject. In CD8+ lymphocyte, significant stronger stimulation of SLA was evident later in the proliferation process. The mean number of divisions in both CD4+/CD8+ lymphocytes stimulated with SLA was significantly greater than when stimulated with live L. major (P=0.007 / P=0.012, respectively) Conclusion: The percentage of divided cells might be calculated separately in each division. The cells remained active following CFSE staining and there is possibility of functional analysis simultaneously.
Identification of Malassezia species associated with seborrheic dermatitis using PCR-RFLP
Mahnaz Mahmoudi Rad,Akram Miramin Mohammadi,Parviz Tousi,Amirhoushang Ehsani
Dermatology and Cosmetic , 2011,
Abstract: "nBackground and Aim: Malassezia is a lipophilic and dimorphic fungus which has different species. Some of them can be found as natural flora on the skin and in some conditions may cause seborrheic dermatitis. The aim of this study was to identify Malassezia species associated with seborrheic dermatitis in Iranian patients, using PCR-RFLP."n"nMethods: In this study out of 79 patients with seborrheic dermatitis, isolates of 70 patients were positive for Malassezia species using PCR-RFLP. The Internal Transcribed Spacer 2 (ITS2) region was amplified by PCR employing the ITS3 and ITS4 primers and The restriction endonucleases AluI, BanI and MspAI were selected for producing distinct RFLP patterns."n"nResults: M. globosa (48.6%), M. furfur (40.0%), M. slooffiae (8.6%) and M. sympodialis (2.8%), were the microorganisms responsible for the infection among participants. M. pachydermatis, M. japonica, M. dermatis, M. restricta, M. obtuse, M. nana and M. yamatoensis were not isolated from any samples."n"nConclusion: Our findings suggest that the most common Malassezia species associated with seborrheic dermatitis was M. globosa, followed by M. furfur.
Identification of Malassezia species associated with pityriasis versicolor using PCR-RFLP
Mahnaz Mahmoudi Rad,Akram Miramin Mohammadi,Parviz Tousi,Ali Khamesipour
Dermatology and Cosmetic , 2011,
Abstract: "nBackground and Aim: Malassezia is a lipophilic and dimorphic fungus which has different species. Some of them can be found as natural flora on skin and in some conditions may cause pityriasis versicolor. The aim of this study was to identify Malassezia species associated with pityriasis versicolor in Iranian patients, using PCR-RFLP."n"nMethods: In this study out of 65 patients with pityriasis versicolor to have pityriasis versicolor,isolates of 60 patients were positive. Malassezia species. using by PCR-RFLP. The Internal Transcribed Spacer 2 (ITS2) region was amplified by PCR employing the ITS3 and ITS4 primers and The restriction endonucleases AluI, BanI and MspAI were selected for producing distinct RFLP patterns."n"nResults: M. furfur (36.7%), M. globosa (30.0%), M. sympodialis (20.0%), M. slooffiae (8.3%), M. restricta (3.3%) and M. obtusa (1.7%) were the microorganisms responsible for the infection among participants. The M. sympodialis infection was strongly correlated with the female gender (P=0.02)."n"nConclusion: Our findings suggest that, the most common Malassezia species associated with pityriasis versicolor was M. furfur, followed by M. globosa.
Phenotyping of peripheral memory T cell subsets in cutaneous leishmaniasis
Ali Khamesipour,Mahmoud Nateghi Rostami,Hossein Keshavarz,Akram Miramin Mohammadi
Dermatology and Cosmetic , 2010,
Abstract: "nBackground and Aim: The heterogenous population of memory T lymphocytes is distinguished based on surface markers and effector functions such as cytokine secretion. Recently, two subsets of memory T cells are defined by expression of chemokine receptor CCR7 and CD45RA designating as "central memory" T cells (TCM) and "effector memory" T cells (TEM). The objective of this staudy was to evaluate the phenotype and function of these lymphocytes in healed cases of cutaneous leishmaniasis."n"nMethods: The phenotype of lymphocytes were determined in blood samples of 13 volunteers with history of self healing cutaneous leishmaniasis (HCL) and in 6 healthy controls."n"nResults: No significant difference was found in memory T cell subsets between HCL volunteers and healthy controls using flow cytometry. However, following sorting of different memory subsets, a significantly higher proliferation was seen in cells of HCL volunteers comparing to the control group. A significantly higher IFN-γ response in TEM and a significantly higher IL-2 response in TCM were observed in cell culture of HCL volunteers comparing controls."n"nConclusion: The responses were elicited when the cells were stimulate with SLA in vitro, it is concluded Leishmania-specific TEM and Leishmania-specific TCM subsets exist in HCL volunteers and since the volunteers with history of CL presumed to be protected against reinfection, it seems that both TCM and TEM play role in the protection against Leishmania infection in these individuals.
Recurrent Pulmonary Tumoral Embolism and Sudden Death as the Presenting Symptom of Wilms’ Tumor
A. Mohammadi
Iranian Journal of Radiology , 2010,
Abstract: Only 4-6% of children with renal tumor show inferior vena caval (IVC) or right atrial infiltration at"npresentation. Tumor emboli are even rarer, and so far, sudden death as the presenting symptom"nhas only been described at presentation in Wilms' tumor (WT) in eight cases. We report a unique"ncase of Wilms' tumor that presented with small pulmonary emboli and im-mediately after"nasthatwas diagnosed before death by detection of small emboli and immediately after sudden"ndeath as massive pulmonary embolism. It seems that in cases of invasive vascular infiltration with"na stable hemodynamic condition at diagnosis immediate surgery may be necessary.
A Retained Wooden Foreign Body in the Lung Parenchyma Presenting as Aspergiloma
A. Mohammadi
Iranian Journal of Radiology , 2010,
Abstract: Despite advances in imaging techniques, the detection of retained wooden foreign bodies remains a difficult and challenging task. The detection of wood is especially important because it may serve as a nidus for superimposed infection.The initial examination may mimic malignancy. We report a rare case of retained wooden foreign body in the lung parenchyma, which was found on CXR and CT as a thick-walled cavity. Surgical resection was performed and the wooden particle was removed. Review of literature shows that wooden foreign bodies in the lung parenchyma are quite rare and they may present with a wide variety of density.We conclude that foreign body should be considered in the differential diagnosis of unusual lung opacity
Soft TissueNon-OpaqueForeignBody:UltrasonographicFindings
A. Mohammadi
Iranian Journal of Radiology , 2010,
Abstract:
Clinical Use of Intravenous Immunoglobulin in Immunodeficient Patients
A Agha Mohammadi
Iranian Journal of Pediatrics , 2000,
Abstract: Intramuscular immunoglobulin products were first used for the prophylaxis of viral diseases, such as measles, infectious hepatitis and poliomyelitis. When the first patient with agammaglobulinemia was described by routine in 1952, it became apparent that immunoglobulin replacement therapy was the treatment of choice for this entity. The intramuscular injections remained the mode of administration for immunodeficiency patients for almost 20 years. In the late sixties it was realized that administration of higher doses of gamma globuline was clinically more effective than administering low doses. As serum levels of IgG within the normal range could not be achieved using products intended for intramuscular use, in the following decades a number of different preparations intended for intravenous use were manufactured and became available. General experience suggests that through (Preinfusion) IgG levels should be maintained at 400-500 mg/dl to achieve satisfactory clinical status. The recommended dose for IVIG replacement in patients with antibody deficiency syndromes is a loading dose of 400-800 mg/kg and subsequent maintenance dose of 350-500 mg/kg every 3-4 weeks. Adverse reactions may be mild, moderate or severe. Mild adverse reactions (Headache, flushing, chills, low back pain, nausea) are often associated with fast infusion rate, and respond rapidly to slowing the infusion. Moderate adverse reactions (Chest pain, wheezing, mild cyanosis), and severe adverse reactions (Bronchoconstriction, severe hypotension, collapse) require the infusion to be discontinued. Antihistamines, hydrocortisone and adrenaline are used as treatment for such adverse reactions. Patients with antibody deficiency and complete absence of IgA, may develop anti-IgA after infusion of immunoglobulin containing IgA, and life-threatening anaphylaxis may occur. Such patients should receive an IgA-free preparation of immunoglobulin. To the present date on immunoglobulin preparation has been found to transmit retroviral infection. However, several preparations have been associated with outbreaks of non-A, non-B hepatitis, including hepatitis C. diagnosis of infection should be based on serum HCV-RNA detection by RT-PCR. Once the immunoglobulin preparation has been selected for a patient, the minimal risks of viral transmission and possible adverse reactions to IVIG therapy should be discussed with parents and patients. Liver transminase concentrations, serum creatinine concentrations, and anti-IgA antibody titers should be measured as baseline and then every six months.
A survey of common prevalent psychiatric disorders among a group of Iranian repatriated prisoners of war
Nourbala A,Mohammadi M
Tehran University Medical Journal , 1995,
Abstract: The present survey covers a number of 91 Iranian repatriated war prisoners who, six months after their freedom, approached three psychiatric clinics in Tehran during 1989 and spring 1990. Of these, 34 persons (37.4%) who showed higher symptoms of disorders were hospitalized and 57 (62.6%) who showed milder signs of disorders were carefully diagnosed mentally and psychologically as outpatients. The prevalent disorders revealed by this study were adjustment disorders (48.3%), mood disorders (22%), schizophrenia (11%), anxiety disorder (9.9%) and organic mental disorders (7.7%). The survey showed statistically the existence of a meaningful relation between the disorder severity with such factors as negative opinion of the prisoner's family on his going to the war front, and observance of martyrdom of co-fighters by the patient. However, no significant relation was observed between the severity of disorders and such factors as being involuntarily dispatched to the front, duration of captivity, type of being captive (singular or in group), having a previous record of solitary imprisonment, observing the treason of co-fighters during his captivity.
FEBRILE SEIZURE: RECURRENCE AND RISK FACTORS
A. TALEBIAN,M. MOHAMMADI
Iranian Journal of Child Neurology , 2006,
Abstract: Background:Febrile Convulsion is the most common convulsive disorder in children,occurring in 2 to 4% of the pediatric population and recurring in 30-50% of cases. Considering the varying recurrence rates reported, thisstudy was conducted at the pediatric ward of the Shaheed BeheshtiGeneral Hospital, between 2000-2001 to determine the frequencyof recurrence and related risk factors in children presenting with theirfirst episode of febrile convulsionMaterials & Methods:A two–year cohort study was performed on 50 children presentingwith the first attack of febrile convulsion. Patient demographic dataincluding age, sex, type and duration of seizure, family history offebrile seizure or epilepsy and the interval between fever onset andoccurrence of seizure were recorded in questionnaires. Those patients,for whom prophylactic medication was not administered, werefollowed at three–month intervals for up to one year. Findings werestatistically analyzed using Fisher’s exact testResults:Recurrence was observed in twelve children (24%) out of the fifty,being most common in patients aged less than one year (54.4%).Recurrence rates among children with a positive family history offebrile convulsion, presence of complex febrile seizure and positivefamily history of epilepsy were 42.1%, 42.8% and 25% respectively.From among those children with a “less than one hour” intervalbetween fever onset and occurrence of seizure, recurrence occurredin 43-7% of cases, while in those with a “more than one hourinterval”, 14.7% experienced recurrence.Conclusion:Recurrence rates are increased by certain factors including age-belowone year-, positive family history of febrile convulsion, and a “lessthan one hour” interval between time of fever onset and seizureoccurrence.
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