oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 15 )

2019 ( 898 )

2018 ( 1052 )

2017 ( 996 )

Custom range...

Search Results: 1 - 10 of 594044 matches for " . María Teresa; Lubián Caballero "
All listed articles are free for downloading (OA Articles)
Page 1 /594044
Display every page Item
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1
Cruz Sui,Otto; Pérez Guevara,. María Teresa; Lubián Caballero,Ana Luisa; Montano Tamayo,Lucy; Silva Cabrera,Eladio;
Revista Cubana de Medicina Tropical , 2007,
Abstract: the objective of this report was to evaluate oral fluid and urine as optional samples for hiv-1 confirmatory test using davih-blot system (laboratorios davih, la habana, cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. in band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. these results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for hiv-1 antibody confirmation testing.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Ana Luisa Lubián Caballero,Héctor Manuel Díaz Torres,Eladio Silva Cabrera,María Teresa Pérez Guevara
Revista Cubana de Medicina , 1998,
Abstract: Se estudiaron, en el período comprendido desde 1991 hasta 1996, 26 352 muestras de suero procedentes de diferentes grupos de riesgo y donantes de sangre para conocer la seroprevalencia de anticuerpos contra el virus linfotrópico de las células T humanas tipo I (HTLV-I) y como continuación de investigaciones realizadas entre 1989 y 1990. Se empleó el sistema de ELISA DAVIH-HTLV-I para el pesquisaje de anticuerpos y como prueba confirmatoria, el Western Blot DAVIH-Blot HTLV-I, ambos de Laboratorios DAVIH, Cuba. Se confirmó la presencia de anticuerpos anti HTLV-I en 10 personas y en la mayoría de ellas el estudio epidemiológico logró esclarecer la vía probable de contagio. El índice de seropositividad observado fue de 0,037 %, lo que en comparación con las tasas de seroprevalencia reportadas para el área del Caribe resulta bajo. In the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human T-lymphotropic virus type I (HTLV-I), and also as a continuation of researches carried out between 1989 and 1990. The ELISA DAVIH-HTLV-I system was used for the antibodies investigation, and as a certifying test, the Western Blot DAVIH-Blot HTLV-I was used too, both from the Cuban DAVIH Laboratories. The presence of anti HTLV-I antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. The seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the Caribbean area.
Diagnóstico serológico del VIH-1 en muestras de sangre seca en papel de filtro por el sistema DAVIH Dot VIH-1
René Grana Sánchez,María Teresa Pérez Guevara,Ana Luisa Lubián Caballero,Héctor Díaz Torres
Revista Cubana de Medicina Tropical , 1998,
Abstract: Se evaluaron muestras de sangre seca en papel de filtro por el sistema DAVIH Dot VIH-1 de Laboratorios DAVIH (La Habana, Cuba). Las muestras utilizadas, 103 positivas y 105 negativas, estaban confirmadas de forma previa por DAVIH Blot de Laboratorios DAVIH. Para utilizar la sangre seca en papel de filtro, se realizaron modificaciones al procedimiento original en cuanto a los tiempos de incubación de las muestras y conjugado, y la agitación en ambas etapas. Se obtuvieron resultados de sensibilidad y especificidad relativas de 100 y 99,05 %, respectivamente. Dried blood specimens spotted on filter paper were evaluated by the HIV-1 DAVIH Dot system from DAVIH Laboratories (Havana, Cuba). The samples used, 103 positive and 105 negative, were previously confirmed by DAVIH Blot from DAVIH Laboratories. In order to use the dried blood on filter paper some modifications were made to the original procedure regarding the times of incubation of the samples and conjugate, and the agitation in both stages. Relative sensitivity and specificity of 100 and 99.05 %, respectively, were attained.
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1 Oral fluid and urine as alternative samples in confirmatory testing of HIV-1 infection
Otto Cruz Sui,. María Teresa Pérez Guevara,Ana Luisa Lubián Caballero,Lucy Montano Tamayo
Revista Cubana de Medicina Tropical , 2007,
Abstract: Se evaluó el fluido oral y la orina como muestras opcionales en el diagnóstico confirmatorio del VIH-1 con el sistema DAVIH-BLOT (Laboratorios DAVIH, La Habana, Cuba), para lo cual se comparó con las correspondientes muestras de sueros en un grupo de 125 individuos. En el análisis de los patrones de bandas reveladas, los anticuerpos predominantes en las muestras positivas de fluido oral y orina contra la p34, p68, gp41 y gp120, no tuvieron diferencias con sus correspondientes sueros según la prueba de comparación de proporciones (p< 0,001). La sensibilidad y especificidad relativas del sistema resultaron de 100 % en el análisis del fluido oral; 98,75 y 100 %, respectivamente, en las muestras de orina. Estos resultados apoyan el empleo opcional del fluido oral y la orina que, con ligeras modificaciones introducidas en el sistema diagnóstico, pueden aplicarse para la confirmación de anticuerpos contra el VIH-1. The objective of this report was to evaluate oral fluid and urine as optional samples for HIV-1 confirmatory test using DAVIH-BLOT system (Laboratorios DAVIH, La Habana, Cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. In band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). Relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. These results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for HIV-1 antibody confirmation testing.
Diagnóstico serológico del VIH-1 en muestras de sangre seca en papel de filtro por el sistema DAVIH Dot VIH-1
Grana Sánchez,René; Pérez Guevara,María Teresa; Lubián Caballero,Ana Luisa; Díaz Torres,Héctor; Montano Tamayo,Lucy;
Revista Cubana de Medicina Tropical , 1998,
Abstract: dried blood specimens spotted on filter paper were evaluated by the hiv-1 davih dot system from davih laboratories (havana, cuba). the samples used, 103 positive and 105 negative, were previously confirmed by davih blot from davih laboratories. in order to use the dried blood on filter paper some modifications were made to the original procedure regarding the times of incubation of the samples and conjugate, and the agitation in both stages. relative sensitivity and specificity of 100 and 99.05 %, respectively, were attained.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Lubián Caballero,Ana Luisa; Díaz Torres,Héctor Manuel; Silva Cabrera,Eladio; Pérez Guevara,María Teresa; Cruz Sui,Otto; de la Fuente Arzola,José Luis; Navea Leyva,Leonor; Noa Romero,Enrique;
Revista Cubana de Medicina , 1998,
Abstract: in the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human t-lymphotropic virus type i (htlv-i), and also as a continuation of researches carried out between 1989 and 1990. the elisa davih-htlv-i system was used for the antibodies investigation, and as a certifying test, the western blot davih-blot htlv-i was used too, both from the cuban davih laboratories. the presence of anti htlv-i antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. the seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the caribbean area.
Pesquisaje de anticuerpos contra el virus linfotrópico de células T humanas tipo I (HTLV-I) en donantes de sangre y grupos de riesgo
ELADIO SILVA CABRERA,MARíA T. PéREZ GUEVARA,ANA L. LUBIáN CABALLERO,JOSé L. DE LA FUENTE ARZOLA
Revista Cubana de Medicina Tropical , 1997,
Abstract: Se estudió la presencia de anticuerpos contra el virus linfotrópico de células T humanas tipo I (HTLV I) en 3 774 sueros; de ellos, 1 409 eran de donantes de sangre, 1 444 de pacientes que habían padecido recientemente alguna enfermedad de transmisión sexual (ETS) y 921 de enfermos politransfundidos. Se emplearon los sistemas DAVIH HTLV I (ELISA) y DAVIH BLOT HTLV I (Western Blot) producidos en Laboratorios DAVIH (La Habana, Cuba), para el pesquisaje y confirmación, respectivamente. De los 68 sueros reactivos en la prueba de ELISA, en 2 se confirmó la presencia de anticuerpos al HTLV I/II y 12 fueron considerados indeterminados por el Western Blot. It was studied the presence of HTLV-I antibodies in 3 774 sera, 1 409 of them were from blood donors, 1 444 were from patients who had suffered from some sexually transmitted disease recently, and 921 from transfused patients. The DAVIH HTLV I (ELISA) and DAVIH BLOT HTLV I (Western Blot) systems produced at the DAVIH Laboratories (Havana, Cuba) were used for screening and confirmation, respectively. Of the 68 reactive sera utilized in the ELISA test, it was confirmed the presence of HTLV I/II antibodies in 2, whereas 12 were considered as undetermined by the Western Blot.
Antigenemia P24: correlación con algunos aspectos clínicos y epidemiológicos en 100 individuos cubanos infectados por VIH-1
Díaz Torres,Héctor Manuel; Ribas Antúnez,María de los ángeles; Lubián Caballero,Ana Luisa; Joanes Fiol,José; Ricardo Fonseca,María Elena;
Revista Cubana de Medicina Tropical , 2001,
Abstract: a non-probabilistic selection of 100 cuban patients at different stages of hiv infection, according to the revised classification of the centers for disease control and prevention of 1987, was made from a set of 130 persons with serologically-confirmed hiv infection. clnical and epidemiological information about each case was collected and peripheral blood samples were taken to detect hiv-1 p24 antigen. the frequency of p24 antigenemia detection and concentration were correlated with available clinical and epidemiological data. p24 antigenemia was significantly more frequent in aids patients. no difference was found between the type of opportunistic diseases diagnosed in the group of patients with detectable p24 antigen and the group that was negative to antigen presence; although in the group with antigenemia concentrations over 100 pg/ml, more than one aids-related disease was often diagnosed simultaneously. a history of sexual intercourses with several persons who had been infected with hiv was much more frequent in patients with antigenemia, and it was associated with a shorter time elapsed from the probable date of infection to the date of their classification as aids patients. these results were compared with the literature review information.
Pesquisaje de anticuerpos contra el virus linfotrópico de células T humanas tipo I (HTLV-I) en donantes de sangre y grupos de riesgo
SILVA CABRERA,ELADIO; PéREZ GUEVARA,MARíA T.; LUBIáN CABALLERO,ANA L.; DE LA FUENTE ARZOLA,JOSé L.; NAVEA LEYVA,LEONOR; CRUZ SUI,OTTO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it was studied the presence of htlv-i antibodies in 3 774 sera, 1 409 of them were from blood donors, 1 444 were from patients who had suffered from some sexually transmitted disease recently, and 921 from transfused patients. the davih htlv i (elisa) and davih blot htlv i (western blot) systems produced at the davih laboratories (havana, cuba) were used for screening and confirmation, respectively. of the 68 reactive sera utilized in the elisa test, it was confirmed the presence of htlv i/ii antibodies in 2, whereas 12 were considered as undetermined by the western blot.
Antigenemia P24: correlación con algunos aspectos clínicos y epidemiológicos en 100 individuos cubanos infectados por VIH-1
Héctor Manuel Díaz Torres,María de los ángeles Ribas Antúnez,Ana Luisa Lubián Caballero,José Joanes Fiol
Revista Cubana de Medicina Tropical , 2001,
Abstract: A partir de 130 personas con infección por VIH-1 confirmada mediante serología se realizó una selección no probabilística de un grupo de 100 pacientes cubanos en diferentes estadios de la infección según la clasificación de los Centros para el Control de Enfermedades revisada en 1987. En cada caso se obtuvo información clínica y epidemiológica y se tomó muestra de sangre periférica para detección de antígeno p24 del VIH-1. Se correlacionó la frecuencia de detección y la concentración de la antigenemia p24 con los datos clínicos y epidemiológicos disponibles. Esta última resultó significativamente más frecuente en los pacientes con SIDA. No se observó diferencia, entre el tipo de enfermedades oportunistas diagnosticadas en el grupo de pacientes con antígeno p24 detectable y el grupo que resultó antígeno negativo; aunque en el grupo con antigenemia a concentraciones superiores a 100 pg/mL se diagnosticó con mayor frecuencia más de una enfermedad indicadora de SIDA simultáneamente. El antecedente de contacto sexual con varias personas antes infectadas con el VIH resultó significativamente más frecuente en el grupo de pacientes con antigenemia y se relacionó con menor intervalo de tiempo desde la fecha probable de contagio hasta la fecha de clasificación como enfermo de SIDA. Estos resultados se compararon con información de la literatura revisada. A non-probabilistic selection of 100 Cuban patients at different stages of HIV infection, according to the revised classification of the Centers for Disease Control and Prevention of 1987, was made from a set of 130 persons with serologically-confirmed HIV infection. Clnical and epidemiological information about each case was collected and peripheral blood samples were taken to detect HIV-1 p24 antigen. The frequency of p24 antigenemia detection and concentration were correlated with available clinical and epidemiological data. P24 antigenemia was significantly more frequent in AIDS patients. No difference was found between the type of opportunistic diseases diagnosed in the group of patients with detectable p24 antigen and the group that was negative to antigen presence; although in the group with antigenemia concentrations over 100 pg/ml, more than one AIDS-related disease was often diagnosed simultaneously. A history of sexual intercourses with several persons who had been infected with HIV was much more frequent in patients with antigenemia, and it was associated with a shorter time elapsed from the probable date of infection to the date of their classification as AIDS patients. These results were compared wi
Page 1 /594044
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.