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Search Results: 1 - 9 of 9 matches for " ?an Mihajla "
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Identification of Glu-B1 alleles in bread wheat cultivars using PCR
Obreht Dragana,Kobiljski Borislav,?an Mihajla,Vapa Ljiljana
Genetika , 2007, DOI: 10.2298/gensr0701023o
Abstract: High dough strength is used as a predictor of good quality bread wheat and it has been attributed largely to the type of allele present at the Glu-D1 locus, where the Glu-D1d allele is most favorable. Recently, it has been observed that cultivars with over expressed subunit Bx7OE at the Glu-B1 have enhanced dough strength. In order to implement recently developed PCR assay for Glu-B1 x-type allele discrimination in marker-assisted selection, a set of wheat cultivars from 11 countries was analyzed. The PCR results matched the known Glu-B1 HMW GS genotypes of the cultivars possessing subunits Bx7 or Bx17. It was shown that this molecular marker also differentiated some other Glu-B1 alleles: Bx6, Bx13, Bx20 and Bx22. These results create the opportunity for an improved method of polymorphism scoring at the Glu-B1 in bread wheat germplasm. .
Marker assisted selection in BMQ related breeding programs
Obreht Dragana,Kobiljski Borislav,?an Mihajla,Vapa Ljiljana
Genetika , 2008, DOI: 10.2298/gensr0801039o
Abstract: Implementation of marker assisted selection (MAS) in bread making quality (BMQ) oriented breeding programs could allow genetic potential assessment of genotypes prior to their phenotypic evaluation. The mechanisms underlying some quality traits in wheat are now understood. This knowledge, coupled with the availability of the DNA sequences of the genes encoding gluten proteins and the wide application of the PCR, has enabled the design of diagnostic DNA markers for these quality traits. Bread wheat breeding programs developed in Institute of Field and Vegetable Crops, Novi Sad have originated a wide range of quality cultivars with strong flours and hard grain texture. During twenty years, in the process of bread-making quality prediction, composition of HMW glutenin subunits were analyzed beside standard technological parameters. However, in order to improve our breeding strategies new generations of PCR-based BMQ related markers were included in selection programs. This paper provides an overview of diagnostic DNA markers that are currently in use in foreign and domestic wheat selection programs. .
Identification of Salicornia population: Anatomical characterization and RAPD fingerprinting
Mili? Dubravka,Lukovi? Jadranka,?an Mihajla,Zori? Lana
Archives of Biological Sciences , 2011, DOI: 10.2298/abs1104087m
Abstract: Anatomical and Random Amplified Polymorphic DNA (RAPD) analysis of two typical populations of Salicornia europaea from Montenegro and Greece (Lesvos), one typical population of S. ramosissima from Spain and one population that belongs to the Salicornia genus from Serbia, was undertaken to develop a new strategy for identifying Salicornia plants. Anatomical variability and differentiation were examined using Principal Component Analysis (PCA) and Multivariate Discriminant Function Analysis (MDA). On the basis of the anatomical measurements, the four populations were classified into three groups: one joining the plants from Serbia and Spain, one comprising the Montenegrin group and one comprising the Lesvos group. RAPD analysis indicated that populations from Spain and Serbia were closely related to each other and the Lesvos group was quite different from all the other investigated populations. These results opened up the possibility that the specimens from Serbia belonged to S. ramosissima and not to S. europaea, as reported previously.
Case-control study of apoE gene polymorphism in young CHD patients and controls in the Serbian population
?an I.,Stoki? Edita,Saka? D.,?an Mihajla
Archives of Biological Sciences , 2011, DOI: 10.2298/abs1101089d
Abstract: Apolipoprotein E displays polymorphism with three common alleles, e2, e3, and e4. The aim of this research was to determine apoE gene polymorphism in a group of healthy patients and a group of patients with CHD, and to reveal the relation between anthropometric and biochemical parameters and the apoE genotype. In CHD group significantly higher values of blood pressure, waist circumference, BMI and fat %, triglycerides, insulin (HOMA IR) and CRP were found. A statistically significant higher presence of the e3e4 genotype and e4 allele was detected in the CHD group. Statistically significant differences between waist circumference, BMI, insulin and HOMA IR were found between subjects with e3e3 and e3e4 genotypes.
HMW glutenin variation and rye chromatine presence in wheat genome
Obreht Dragana R.,Vapa Ljiljana B.,Davidovi? Mihajla R.
Zbornik Matice Srpske za Prirodne Nauke , 2003, DOI: 10.2298/zmspn0305043o
Abstract: For estimation of wheat end-product quality during wheat breeding programs composition of high-molecular-weight glutenin subunits (HMW GS) and the presence of 1BL/1RS translations serve as markers due to their profound effects on dough elasticity and viscous properties. Ninety-three wheat genotypes from Institute of Field and Vegetable Crops in Novi Sad have been analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in order to determine their HMW GS composition and 1BL/1RS translocation presence. Eleven alleles were found at the Glu-1 loci. Subunits 1 and 2*and the null allele N were determined at the Glu-A1 locus. Subunitis 7, 7+9, 7+8, 6+8, 20 and 21 were found at the Glu-B1 locus, subunits 2+12 and 5+10 at the Glu-D1 locus. The 1BL/1RS translocation was discovered in 28 cultivars, although three of them were heterogeneous.
An assessment of the genetic diversity in the wild boar population from the Podunavlje-Podravlje hunting area
Veli?kovi? Nevena,?an Mihajla,Zori? M.,Obreht Dragana
Archives of Biological Sciences , 2010, DOI: 10.2298/abs1003807v
Abstract: nema
Molecular characterization of glutenin alleles at the Glu-Dl locus
Vapa Ljiljana B.,Obreht Dragana R.,Kobiljski Borislav ?.,Davidovi? Mihajla R.
Zbornik Matice Srpske za Prirodne Nauke , 2003, DOI: 10.2298/zmspn0305035v
Abstract: It is well known that the composition of high-molecular-weight (HMW) glutenin subunits impacts the bread making quality. The HMW subunits 1Dx5-1Dyl0 are typically associated with high dough strength and good bread making quality contrary to 1Dx2-lDyl2 subunits. Bread wheat cultivars from Institute of Field and Vegetable Crops in Novi Sad have been screened for the alleles present at Glu-Dl locus using traditional SDS PAG electrophoresis method and a new PCR based approach. The Glu-Dl locus was screened for two main x-type alleles which code for HMW glutenins 2 and 5, and two main y-type alleles which code for HMW glutenin subunits 10 and 12. Among the analyzed cultivars 55.6% expressed the presence of 1Dx5 and 1Dy10 alleles at the Glu-Dl locus. These results confirmed that by using marker-assisted selection (MAS) it is possible to identify genotypes with alleles for good bread making quality which could be successfully used in wheat breeding programs.
Genetic variability of pheasant (Phasianus spp) in breeding station Ristova a
Vapa Ljiljana B.,?an Mihajla R.,Obreht Dragana R.,To?ovi?-Mari? Biljana M.
Zbornik Matice Srpske za Prirodne Nauke , 2004, DOI: 10.2298/zmspn0417005v
Abstract: One of the possible reasons for pheasant population number decline in past several years might be loss of adaptability in populations originated from breeding stations caused by inbreeding depression. Due to fact that adaptability is a consequence of genetic structure of the populations, the aim of this paper was the analysis of genetic variability in pheasant population from breeding station Ristovaca using molecular markers. Allozyme variability of 20 putative gene loci was detected by polyacrylamide gel electrophoresis. Polymorphism was revealed in 5 loci: Est-1, Pgd, Sod, Gpi-2 and Odh. The values of genetic variability measures - heterozigosity polymorphism, fixation indices and H/P ratio indicate low level of genetic variability and possible presence of inbreeding depression within pheasant population.
On gene pool divergence of the brown hare (Lepus europaeus, Pallas) in Vojvodina
?an Mihajla R.,Vapa Ljiljana B.,Obreht Dragana R.,Vapa Milan M.
Zbornik Matice Srpske za Prirodne Nauke , 2004, DOI: 10.2298/zmspn0417013d
Abstract: Today it is widely accepted that a conservation of genetic diversity increases chances of animal survival. The aim of this study was investigation of gene pool divergence of the brown hare (Lepus europaeus) in Vojvodina. Allozymic diversity of 60 brown hares from different localities in Vojvodina was studied by polyacrylamide and starch gel electrophoresis at 31 putative structural gene loci. Only five loci: Idh-2, Pgd, Pgm, Es-1 and Es-D were polymorphic, possessing 2 to 6 different alleles. The value of average heterozygosity (Ho) was 0.0427, while polymorphism (P99%) was 8.4%. Nei's values of genetic distance (ranged from 0.000 to 0.029) and modified Roger's distance (ranged from 0.030 to 0.181) were calculated among hare populations. Apart from the relatively high values of heterozygosity and polymorphism, the nuclear gene pool diversity of brown hare population in Vojvodina based on allozyme variation is low and corresponds to the data obtained for the populations in Austria and Central Europe.
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