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In order to complete the reinforcement design of lock chamber floor, we use 3D finite element analysis of the southern dike lock chamber in Meishan waterway under each working condition by using the ANSYS, calculate the lock chamber structure stress, and then select the maximum stress for reinforcement calculation. The calculated value of reinforcement ratio is lower than the standard value. Study shows that the lock chamber structure based on ANSYS finite element analysis can calculate each part of the complex stressing sluice chamber structure’s stress, providing the basis for reinforcement; that has guiding significance for its design and optimization.