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Search Results: 1 - 10 of 73002 matches for " 韩雪清? "
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抗病毒免疫研究进展*
,刘湘涛
微生物学通报 , 2002,
Abstract: 抗病毒免疫的机制极其复杂,宿主的免疫系统需要经过抗原肽的加工提纯、淋巴细胞活化、抗体和细胞因子的产生等一系列步骤才能最终清除病毒的感染。从蛋白酶体在病毒肽抗原加工中的作用、免疫支配效应的意义、CTL杀伤病毒感染细胞的机制、细胞因子的作用和免疫逃逸机制等方面阐述了抗病毒免疫机制,对于病毒的防治将是十分重要的。
减小来自阴极保护装置杂散电流干扰的实验探究
Experiments to Explore the Decrease of Stray Current Interference from Cathode Protection
 [PDF]

汪洋, 张亚萍, 秀虹, , 句东旭, 于濂, 李焰
Modern Physics (MP) , 2015, DOI: 10.12677/MP.2015.53009
Abstract:
采用实验室模拟的方法,研究在管线交叉处使用牺牲阳极和涂层保护两种措施的实验效果,将两种措施减小或消除来自阴极保护设施杂散电流干扰的实验效果与用计算机软件ANSYS模拟的结果进行比较,进而为消除来自阴极保护设施杂散电流对交叉管线的腐蚀影响提供一定的科学地依据。
This paper adopts the method of laboratory simulation to research the effect that using anode in intersections and using coating have on reducing or eliminating the interference of stray current from cathodic protection facilities, and compares the experiment results with the results of computer simulation software ANSYS. Then, this paper provides some scientific basis for eliminateing stray current corrosion influence from cathodic protection facilities to cross pipeline.
近距离煤层下保护层开采保护范围的确定
赵训,真理,李树,解庆
煤炭科学技术 , 2013,
Abstract: 为确定金佳矿中煤组近距离煤层群下保护层11223工作面开采的保护范围,在111811运输巷和回风巷共布置6组瓦斯解吸钻孔。通过现场测定的钻孔瓦斯解吸量,结合实验室煤层瓦斯参数计算出瓦斯压力。对瓦斯压力回归分析,得出6组保护范围临界点为理论保护范围,即分别向钻孔内偏移1.30、1.10、17.51、16.52、3.20和3.90m;走向卸压角55°、倾向卸压角分别为54°和82°。保护范围内被保护煤层卸压瓦斯抽采纯量约2628.63万m3,抽采率80.69%;钻屑瓦斯解吸指标未超标。
悬浮芯片技术应用进展
程涛,王慧煜,梅琳,
生物技术通报 , 2011,
Abstract: 悬浮芯片技术(SAT)是一种新型、高通量的生物芯片技术,它是将流式细胞术、激光技术及应用流体学等技术结合在一起,利用悬浮在液相中的分类荧光编码微球作为检测载体,具有高通量、速度快、灵敏度高、特异性强及检测范围广等特点。近几年来,悬浮芯片技术在免疫学、基因组学、蛋白质组学及临床诊断检测等方面应用较广泛。就其原理、技术特点和应用作一介绍。
醋酸甲羟孕酮人工抗原的合成与鉴定
杨泽晓?,张彦明?,
西北农林科技大学学报(自然科学版) , 2008,
Abstract: [目的]为建立醋酸甲羟孕酮(mpa)快速准确的免疫学分析技术,对mpa人工抗原的合成鉴定进行研究.[方法]将mpa肟化后与琥珀酸酐反应制得mpa半琥珀酸(mpa-hs),然后通过碳二亚胺法将mpa-hs与牛血清白蛋白(bsa)偶联合成人工抗原mpa-bsa,并采用紫外全波长扫描法、sds-page法、elisa检测法和动物免疫试验对其进行鉴定.[结果]mpa与bsa的偶联比为8:1时,合成的人工抗原mpa-bsa具有良好免疫原性与反应原性.[结论]成功合成了人工抗原mpa-bsa,为进一步的mpa免疫学分析技术研究奠定了基础.
广西猪瘟流行毒与c-株疫苗毒gp55(e2)主要抗原区dna序列差异的比较
张永国?,刘湘涛?,
西北农林科技大学学报(自然科学版) , 2002,
Abstract: 采用反转录pcr(rt-pcr)和套式pcr(nestpolymerasechainreaction,npcr)扩增出3株广西省近期(1999~2000年)猪瘟流行野毒的e2基因,将其分别克隆于pmd-18t载体上并进行了核苷酸序列测定,根据c株及brescia和alfort株确定起始氨基酸三联体的正确位置后进行氨基酸序列推导,同时进行了同源性比较及e2糖蛋白结构的分析。结果表明,所测3株hcve2基因的长度均为1170bp,编码的氨基酸序列均包括完整信号肽序列和部分跨膜序列,共由384个氨基酸组成。3株流行毒的e2基因核苷酸序列同源性为90.10%~98.54%,相应的氨基酸序列同源性为89.59%~97.92%。这3株流行毒与c-株兔脾组织毒(疫苗种毒)e2基因的核苷酸序列同源性为82.87%~83.99%,相应的氨基酸序列同源性为86.98%~90.10%,表明近期猪瘟流行毒与c-株疫苗毒的gp55蛋白之间存在一定的差异。
极具有应用前景的生物学检测技术-生物传感器
,杨泽晓,林祥梅
中国生物工程杂志 , 2008,
Abstract: 生物传感器是近年逐渐发展起来的一种高新生物学分析检测技术,它将生物学或仿生学信号感应部件紧密连接或整合到传感系统内,具有特异、敏感、快速、便携以及操作简便等优点,发展非常迅速,并且被应用到医疗保健、食品工业、畜牧兽医等多个领域,已成为人们研究的热点之一。本文概述了生物传感器的概念与工作原理、分类、与主要领域的研究应用,分析了生物传感器的产业现状,优点与现存问题,并对其应用发展前景进行了展望。
六盘山区旱作春玉米养分投入与肥料生产效率
Nutrient input and fertilizer production efficiency analysis for dry-cultivation spring maize in dryland of Liupanshan area

夏海,,张旭东,赵冀,杨琳,张鹏,
- , 2018, DOI: 10.7606/j.issn.1000-7601.2018.04.06
Abstract: 为了明确半干旱地区玉米生产养分投入状况及肥料生产效率,提高旱地农业资源效率,连续三年对宁夏六盘山区不同类型旱地玉米施肥及生产状况进行调查。调查结果显示:六盘山区玉米生产中的氮肥(纯N)、磷肥(P2O5)和钾肥(K2O)的平均投入量分别为323.7、210.1 kg·hm-2和28.6 kg·hm-2,氮、磷、钾比例为1∶0.64∶0.08,磷的比例偏高,而钾肥比例偏低;氮、磷肥施肥量明显偏高,分别集中在高于330 kg·hm-2和高于165 kg·hm-2水平的农户比例高达48.5%和70.1%,造成了浪费和环境污染,钾肥用量偏低,集中处于低于42 kg·hm-2水平,样本比例高达69.3%;施肥基追比大致为6∶4,追肥主要为尿素,平均用量为121.9 kg·hm-2,适宜玉米生长,但施追肥量处于两个极端,大多数追肥量或者很高或者很低;调研区玉米三年平均产量为9 864 kg·hm-2,由于降雨不足与施肥不合理,玉米产量呈逐年递减趋势;氮肥、磷肥和钾肥的三年平均偏生产力分别为30.7、47.8 kg·kg-1和391.3 kg·kg-1,氮肥和磷肥的偏生产力偏低。该区域玉米生产有机肥投入以牛羊粪便为主,投入量偏低。综上所述,调研区施肥方面存在投入量和比例不合理问题,需进行科学指导与调整。
In order to explore the nutrient input and efficiency of fertilizer use of maize production in semi-arid region. A three-year consecutive investigation and analysis was carried out in Liupanshan area. The result showed that: (1) Average input of pure N, P2O5, and K2O fertilizer in maize production were 323.7 kg·hm-2, 210.1 kg·hm-2 and 28.6 kg·hm-2 respectively; The ratio of pure N, P2O5, and K2O fertilizer was 1:0.64:0.08, The the proportion of Pfertilizer was higher, and K fetilizer was lower; (2) Dosage of N and P fertilizer were higher, and the percentage of farmers who applied fertilizer higher than 330 kg·hm-2 and 165 kg·hm-2 of N and P fertilizer were 48.5% and 70.1% respectively, which was a waste and would cause environment pollution; Use of KFertilizer was lower, the percentage of K fertilizer application was lower than 42 kg·hm-2 was as high as 69.3%. The topdressing fertilizer was mainly urea, and its average dosage was 121.9 kg·hm-2, the ratio of basal and topdressing fertilizer was approximately 6∶4, which suited maize growth. But the individual topdressing dosage had two extreme, in most cases it was very high or very low. (3) The average yield of maize in research area in three years was 9 864 kg·hm-2. Due to the poor rainfall and irrational fertilizer application from 2013 to 2015, the maize yield declined year by year;(4) The PFP(Partial fertilizer productivity ) of pure N, P2O5, and K2O fertilizer was 30.7 kg·kg-1, 47.8 kg·kg-1 and 391.3 kg·kg-1 respectively in the three years, the PFP of pure N, P2O5 were lower. The input of organic fertilizer in the research area was mainly cow and sheep dung, and it’s low. In conclusion, in Liupanshan area, the input dosage and proportion of fertilizer were irrational, which need a scientific guidance and adjustment.
猪瘟病毒E2基因在Pichia pastoris中的表达及其免疫活性的初步研究
,刘湘涛,张涌,谢庆阁,田波
生物工程学报 , 2002,
Abstract: 将猪瘟病毒的E2基因克隆入酵母分泌型表达载体pPIC9K中,酶切线性化后电穿孔导入Pichia pastoris进行整合,经G418筛选得到高拷贝转化子,甲醇诱导表达。SDSPAGE和Western blot结果证实了酵母培养上清液中含有E2蛋白。免疫活性研究证明P. pastoris表达的E2蛋白能刺激动物产生抗猪瘟病毒的抗体。
Expression of Antigenic Region of VP1 Gene of Swine Vesicular Disease in Escherichia coli
猪水泡病病毒VP1基因抗原区的原核表达

张永国,刘湘涛,,张彦明,谢庆阁
微生物学报 , 2003,
Abstract: 利用RTPCR和nested PCR(nPCR)技术扩增出猪水泡病病毒VP1基因的抗原区,将其克隆到表达载体pProEXHTb中,获得重组质粒,经PCR、酶切和序列分析鉴定表明,目的基因插入的位置、大小和读码框均正确。将重组质粒导入BL21(DE3),经IPTG诱导表达后SDSPAGE检测表明,重组菌能表达猪水泡病病毒VP1抗原区蛋白;Western blot检测表明,诱导表达的抗原区蛋白能与猪水泡病阳性血清发生特异性反应。
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