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Search Results: 1 - 10 of 210491 matches for " 陈永枫 "
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钢筋混凝土柱的分节装配

工业建筑 , 1964, DOI: 10.13204/j.gyjz196409009
Abstract: 冶金工业厂房,一般都有三大特点跨度大、厂房高、吊车重。对厂房结构的强度、刚度和耐久性都有较高的要求。因此,厂房的主要承重结构都比较大而重,特别是钢筋混凝土柱,一般都在30吨上下,甚至有的超过50吨。大型预制钢筋混凝土柱的搬运和吊装,对一般施工单位来就是存在一定困难的。我们于1961年在改建某炼钢厂工程中,由于钢
板基法向冻胀力现场试验研究
,周长庆,,肖柏
冰川冻土 , 1981,
Abstract: 迄今,国内外有关基底法向冻胀力的资料大多局限于小面积,现场大面积之实测值所见较少,难于在大型板基中应用。本文根据就地试验观测结果,对基底法向冻胀力与基础面积间的关系及分布情形进行了讨论和分析。
甜瓜抗蔓枯病种质资源的筛选及rapd分析
周晓慧,joseph,n.,wolukau,李英,,吴明珠,
园艺学报 , 2007,
Abstract: 采用甜瓜蔓枯病病菌孢子悬浮液对208份甜瓜种质资源进行苗期人工接种鉴定,发现86份材料表现抗病,122份材料表现感病。用rapd标记对抗性表现最优的29份材料进行分析,14个引物共扩增出116条多态性条带,平均每个引物为8.29条。shannon多样性指数和nei,s基因多样性指数分别为0.4331和0.2855,表明这些抗源材料遗传差异较大。upgma聚类分析将这29份抗性种质明显划为普通型和野生型两大类,种质间还表现出一定的地域特性。
黄瓜扩张素蛋白基因csexpb1的克隆及表达分析
,李季*,徐建,张开京,娄群峰,
园艺学报 , 2015, DOI: 10.16420/j.issn.0513-353x.2014-0958
Abstract: 基于黄瓜果实转录组学研究,筛选并克隆了一个扩张素蛋白(β-expansin)基因csexpb1。蛋白序列同源比对发现csexpb1蛋白是一类钙调素类似蛋白(calmodulin-likeprotein,cml),与甜瓜、草莓及番茄扩张素蛋白基因具有较高的相似性;expasy分析发现csexpb1为亲水性蛋白,亚细胞定位分析证明该蛋白广泛分布在细胞质以及细胞核结构中;qrt-pcr分析发现csexpb1无论是在授粉坐果还是单性结实坐果过程中皆上调表达,而在不授粉败育的果实中下调表达;qrt-pcr也表明csexpb1的表达受到生长素、细胞分裂素和赤霉素等单性结实诱导激素的正向调控,推测csexpb1与黄瓜坐果有关,可能参与了激素诱导的单性结实过程。
单倍体甜瓜离体繁殖的方法
,伊鸿平,贾媛媛,冯炯鑫,吴明珠,
园艺学报 , 2007,
Abstract: 以单倍体甜瓜无菌苗的顶芽和腋芽为外植体进行离体繁殖研究。结果表明:单倍体甜瓜最佳增殖培养基为ms+6-ba0.5mg·l-1,最适生根培养基为1/2ms+iba0.5mg·l-1,增殖系数为3.57。染色体计数表明没有倍性变化发生。
不同胁迫处理方法对结球甘蓝GABA含量的影响
何娜,叶晓,李丽倩,李广胜,沁滨,
南京农业大学学报 , 2013, DOI: 10.7685/j.issn.1000-2030.2013.06.018
Abstract: 以结球甘蓝(Brassicaoleraceavar.capitataL.)为试材,研究不同机械切分大小、4℃低温贮藏和浸泡胁迫处理对γ-氨基丁酸(GABA)含量和谷氨酸脱羧酶(GAD)活性的影响。结果表明机械切分、低温贮藏和浸泡处理均可提高GABA含量和GAD活性。切分大小为0.5cm×3.0cm的外叶和内叶中GABA含量最高,分别为1.20mg?g-1和1.88mg?g-1,内叶显著高于外叶。4℃下贮藏1.5h时外叶和内叶中的GABA含量达到最大值,分别为1.77和2.41mg?g-1,且对应的外叶中GAD活性和GABA含量显著低于内叶。100μmol?L-1CaCl2溶液浸泡2h,内叶GABA含量为2.32mg?g-1,外叶为1.52mg?g-1。在pH5.6的磷酸-柠檬酸缓冲液中浸泡2h,内叶GABA含量最高为2.01mg?g-1,而外叶在pH6.0时达到最大值1.47mg?g-1。10g?L-1谷氨酸钠浸泡处理4h时GABA含量显著增加,内叶为1.36mg?g-1,外叶为1.04mg?g-1,内叶显著高于外叶;浸泡过程中,内叶GAD活性较外叶变化显著。
低温对不同基因型黄瓜幼苗生长及生理特性的影响
,宁宇,魏庆镇,
华北农学报 , 2013, DOI: 10.7668/hbnxb.2013.06.025
Abstract: 人工低温条件下比较了EC1、北京截头和SWCC123种黄瓜苗期在形态、生理及PSⅡ对低温响应的差异。结果表明:低温胁迫均使3种黄瓜产生冷害,EC1植株脱水最轻,SWCC12冷害最严重;3种黄瓜的电渗率和丙二醛(MDA)因低温处理而增加,变化最小的是EC1,最大的是SWCC12;低温处理提高了3种黄瓜中的超氧化物歧化酶(SOD)活性,尤其是EC1和北京截头的SOD活性反应更为高效和快速,EC1中过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)呈先增加后降低趋势,但始终高于处理前的,而北京截头中分别先降低后升高及变化不明显,SWCC12中主要变化趋势为增加,EC1体内的过氧化氢酶(CAT)在低温处理2d时活性最高,之后下降至原来水平,北京截头和SWCC12中变化不大;3种黄瓜体内可溶性蛋白在胁迫处理时都呈先升高后降低趋势;EC1和北京截头中的可溶性糖也表现出先升高后降低现象,但都显著高于处理前,而SWCC12中可溶性糖呈显著下降趋势;3个基因型黄瓜内叶绿素含量和类胡萝卜素含量随胁迫时间先增加后降低,EC1中维持高含量时间长,下降幅度最小,SWCC12下降幅度最大;低温均影响了3份黄瓜的PSⅡ、Fv/Fm、Yield和ETR,下降幅度最大的是SWCC12、最小的是北京截头。
黄瓜遗传转化体系优化的研究
李蕾,,张璐,娄群峰,李季,钱春桃,
华北农学报 , 2015, DOI: 10.7668/hbnxb.2015.05.019
Abstract: 为了提高农杆菌介导的黄瓜遗传转化效率,以亚洲生态型长春密刺、欧洲生态型Poinset76和Marketmore763种黄瓜为材料,筛选了适合遗传转化最佳的基因型,并通过改变培养基固化剂、添加抗氧化剂和有机添加物的方式对遗传转化体系进行优化。结果发现,3种基因型黄瓜中,长春密刺的抗性芽诱导分化表现最佳,而Poinset76和Marketmore76稍差;在脱乙酰吉兰糖胶为固化剂的培养基上,3种基因型的抗性芽诱导率比以琼脂粉为固化剂的培养基诱导率分别高28.21,19.71,15.39个百分点;共培养基中添加50mg/L抗氧化剂α-硫辛酸时,长春密刺抗性芽诱导率最高达66.67%,平均芽分化数最高达1.32;在选择培养基中添加1.5g/L的有机添加物水解酪蛋白,长春密刺抗性芽诱导率和平均芽分化数最高,分别为67.15%和1.42。此外,在前人建立的农杆菌介导的遗传转化体系的基础上,得到了以长春密刺为遗传转化的最适基因型,脱乙酰吉兰糖胶为培养基固化剂,共培养基中添加50mg/Lα-硫辛酸或选择培养基中添加1.5g/L水解酪蛋白的优化遗传转化体系,极显著提高了外植体抗性芽诱导率和平均芽分化数,推动了黄瓜转基因进程。
GPS导航超声系统引导下超细经皮肾镜取石术治疗无积水肾下盏结石的临床应用
周酉,齐勇,,樊晓栋,,汤春波
- , 2018, DOI: 10.3969/j.issn.1007-1989.2018.08.019
Abstract: 摘要: 摘要:目的??探讨GPS导航超声系统引导超细经皮肾镜取石术(UMP)治疗无积水肾下盏结石的临床疗效及安全性。方法?回顾性分析2017年1月-2017年9月该院23例采用GPS引导UMP治疗无积水肾下盏患者的临床资料。其中,单发结石17例,多发结石6例。结石大小14.0~25.0?mm,平均(18.3± 3.6)mm,结石CT值880~1?610?Hu,平均(1?253.6±182.8)Hu。俯卧位建立“人工肾积水”后在GPS导航引导下16G穿刺针穿刺入目标肾盏,退出针芯见灌注液流出后,将F3超细肾镜直接沿穿刺针鞘进入肾集合系统,进一步确认穿刺针鞘头端在目标肾盏位置,并找到肾盏颈口,调整穿刺针鞘方向及深度使其头端靠近盏颈口或进入肾盂内。退出超细肾镜,置入斑马导丝,扩张通道至F14后置入F13外鞘,使用超细经皮肾镜进行碎石取石。结果?23例均单通道经下盏穿刺行UMP。定位穿刺时间2~8?min,平均(4.2±1.6)min,一次穿刺成功率为100.00%。手术时间30~65?min,平均(42.3±7.6)min;术后住院天数2~5?d,平均(2.6± 0.5)d;血红蛋白下降0~27?g/L,平均(11.2±5.1)g/L。术后止痛药的使用率为0。术后第1或2天复查腹平片(KUB)评估结石清除率为95.65%(22/23)。所有患者围手术期未出现发热、严重出血、集合系统穿孔、通道丢失和胸膜损伤等并发症。结论?GPS导航超声系统结合UMP治疗无积水肾下盏结石安全、有效,结石清除率高,术后恢复快,值得临床推广应用。
Abstract: Abstract: Objective?To investigate the clinical efficacy and safety of real-time ultrasonography-guided ultra-mini percutaneous nephrolithotomy (UMP) using SonixGPS navigation in treatment of lower caliceal calculi without hydronephrosis.?Methods?From January 2017 to September 2017, 23 cases of lower caliceal calculi without hydronephrosis were treated with UMP under real-time ultrasonography-guided by SonixGPS navigation. 17 cases among them were single stone, while the other 6 cases were multiple renal stones. The mean stone size is (18.3?±?3.6)?mm in diameter, CT value (1253.6?±?182.8)?Hu.?Results?Single-access UMP punctured via lower caliceal was performed in all the 23 cases successfully. The mean puncture time was (4.2?±?1.6)?min; mean operation time was (42.3?±?7.6)?min; mean postoperative hospital stay was (2.6?±?0.5)?d; mean hemoglobin drop was (11.2?±?5.1)?g/L. No major complications were recorded and no analgesic consumption perioperatively. The stone clearance rate was 95.65% (22/23).?Conclusion?Real-time ultrasonography-guided UMP using SonixGPS navigation for the treatment of lower caliceal calculi without hydronephrosis is safe and effective, with high stone free rate and easy recovery, which can be popularized in clinical practice.
黄瓜T-DNA插入突变体库的构建
李蕾,李季,,张璐,娄群峰,钱春桃,
南京农业大学学报 , 2016, DOI: 10.7685/jnau.201504014
Abstract: [目的]为了进一步研究黄瓜基因的功能,本文构建了黄瓜T-DNA插入突变体库。[方法]以黄瓜栽培品种‘长春密刺’子叶节为转化外植体,通过农杆菌介导的遗传转化方法,将T-DNA插入突变载体pROK2后转化黄瓜;通过筛选压力梯度试验,确定遗传转化体系最适合的卡那霉素(Kan)筛选浓度;使用PCR检测和斑点杂交方法鉴定T0和T1代转化植株;以‘长春密刺’植株为对照,统计T1代植株表型。[结果]确定100 mg?L-1为最适合的Kan抗性芽筛选浓度。T0代植株PCR检测结果初步证明,pROK2成功整合到14S1和14S2两个T0代株系基因组中。14S1自交后获得55个T1代单株,对其进行PCR检测,发现其中12个单株均扩增出了35S和NPT-Ⅱ片段。以Dig标记的35S和NPT-Ⅱ为探针,对这12个单株及随机从剩余T1代中选取的11个单株进行斑点杂交检测,得到了2个含有35S和NPT-Ⅱ杂交信号的单株:14S1-27和14S1-34。性状调查统计显示:T1代植株在生长各阶段相对于野生型无明显突变表型。[结论]pROK2重组质粒成功整合到了14S1株系的基因组中。对14S1自交后代的PCR检测和斑点杂交检测结果进一步证明了14S1为转化植株,确定14S1为插入突变体。结合T-DNA插入位点鉴定技术可进一步开展相关基因分离及功能研究工作。
[Objectives]For the purpose of further study on the cucumber gene function,this article carried out the research that is the construction of cucumber T-DNA insertion mutant library. [Methods]With the cotyledon nodes of cucumber cultivars‘Changchunmici’as the explants and the Agrobacterium-mediated transformation method,T-DNA was inserted into vector pROK2 and cucumber was transferred. The most suitable selection pressure concentration of kanamycin was confirmed by setting selection pressure gradient. PCR and dot blotting were used to identify the T0 and T1 transgenic plants. The phenotypes of T1 plants were statistically investigated with ‘Changchunmici’ used as control. [Results]The selection pressure concentration of kanamycin was 100 mg?L-1. PCR detection results of T0 plants indicated that pROK2 was integrated into 14S1 line and 14S2 line successfully. 55 T1 plants were gained from 14S1 self-pollinated and 12 of them could amplify 35S promoter and NPT-Ⅱ gene sequence. The results of dot blotting detection of the 12 plants and 11 plants randomly selected from the other T1 plants by dig labeled 35S probe and NPT-Ⅱ probe were that 14S1-27 and 14S1-34 had 35S and NPT-Ⅱ hybridization signals. Traits investigation showed the phenotype of T1 generation from 14S1 self-pollinated had no difference with‘Changchunmici’in every period of growth. [Conclusions]pROK2 recombinant plasmid was integrated into 14S1 successfully. Results of PCR and dot blotting detection of 14S1 selfed progeny further proved that 14S1 were transgenic plants that are T-DNA insertion mutant. Then gene isolation and function analysis will be studied with T-DNA insertion site identification methods
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