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Search Results: 1 - 10 of 280600 matches for " 陈正华HOU Bing-kai "
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Cloning of Insecticidal Protein Gene from Bacillus thuringiensis and Chloroplast Genetic Transformation in Brassica napus L.
苏云金芽孢杆菌杀虫蛋白基因克隆及油菜叶绿体遗传转化研究 Cloning of Insecticidal Protein Gene from Bacillus thuringiensis and Chloroplast Genetic Transformation in Brassica napus L.

侯丙凯,HOU Bing-kai,CHEN Zheng-hua
遗传 , 2001,
Abstract: 向细胞核导入外源基因的核转化技术是植物基因工程的主要方法。然而,外源基因表达效率低,表达不稳定,基因易失活和因随机插入而造成的位置效应等是该方法不足之处。而且,由于外源基因可随花粉扩散,细胞核转化体的生物安全性问题已在全球范围内引起世人的关注和担忧。将外源基因导入叶绿体基因组有望克服细胞核转化中存在的某些弊端。油菜作为世界上重要的油料作物,其叶绿体遗传转化研究还未见报道。本研究从苏云金芽孢杆菌克隆得到野生型杀虫晶体蛋白基因,构建了用于油菜叶绿体定点转化的植物表达载体,并用基因枪法将杀虫蛋白基因导入油菜,…
植物基因工程表达载体的改进和优化策略 Strategies for Optimizing Expression Vectors Used in Plant Genetic Engineering
侯丙凯,夏光敏,HOU Bing-kai,XIA Guang-min,CHEN Zheng-hua
遗传 , 2001,
Abstract: 外源基因在转基因植物中表达效率低一直是令相关研究者困扰的一个问题.转基因植物的生物安全性最近在全球范围内开始引起世人的担忧.本文简要介绍了近年来在植物表达载体构建方面所采用的一些新策略,这些策略有助于增强外源基因的表达水平、提高生物工程体的安全性。 Abstract:The low expression level of foreign genes in transgenic plants is a puzzling question for researchers in plant genetic engineering.The biosafety concern is now causing a growing public wariness of transgenic plants around the world.This article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering.These strategies are important for obtaining better expression of foreign genes and developing safer transgenic plants.
Plant Regeneration From Protoplasts of Different Types of wheat Callus and Their Derivative Suspensions
小麦不同类型愈伤组织及其悬浮系的原生质体培养和植株再生

Hou Bing-kai,Cheng Ji-jiang,Song Xie-zhi,
侯丙凯
,际江,宋协志

植物学报 , 1996,
Abstract:
Current Research on Transgenic Plants for Conferring Virus Tolerance
植物抗病毒基因工程的某些现状

Hou Bing-kai,Yu Hui-min,
侯丙凯
,于惠敏

植物学报 , 1995,
Abstract:
生物钟的分子机制研究进展 Recent Developments in Molecular Mechanisms of Biological Clock
侯丙凯,于惠敏HOU Bing-Kai,YU Hui-Min
遗传 , 1996,
Abstract: RecentDevelopmentsinMolecularMechanismsofBiologicalClockHouBingkai(DepartmentofBiology,ShandongUniversity,Jinan250100)YuHuimin(DepartmentofBiochemistry,ShandongEducationCollege,Jinan250013)生物的昼夜节奏表现,从单细胞生物到多细胞生物,从原校生物到真核生物都曾被描述过。由于这种现象在生物界广泛存在,关于它的特征、意义和机理的研究日益受到人们重视。其中最重要和最吸引人的方面是它的测时系统—一生物钟(biologicalclock),也称生物振荡器(oscillators)。近年来,人们从分子水平对生物钟的研究比较活…
Expression Vector Used in Chloroplast Genetic Transformation
用于叶绿体遗传转化的表达载体 Expression Vector Used in Chloroplast Genetic Transformation

HOU Bing-kai,YU Hui-min,XIA Guang-min,
侯丙凯
,于惠敏,夏光敏HOU Bing-kai,YU Hui-min,XIA Guang-min

遗传 , 2002,
Abstract: Chloroplast genetic transformation is a new way of plant genetic engineering.This paper reviews the construction methods of expression vector used in chloroplast genetic transformation.It contains the homologous recombinant fragments,the chloroplast specific promoter and terminater,the selectable marker genes and the interest genes whose expression in chloroplast have been achieved.
UGT74D1 Is a Novel Auxin Glycosyltransferase from Arabidopsis thaliana
Shang-Hui Jin, Xin-Mei Ma, Ping Han, Bo Wang, Yan-Guo Sun, Gui-Zhi Zhang, Yan-Jie Li, Bing-Kai Hou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061705
Abstract: Auxin is one type of phytohormones that plays important roles in nearly all aspects of plant growth and developmental processes. The glycosylation of auxins is considered to be an essential mechanism to control the level of active auxins. Thus, the identification of auxin glycosyltransferases is of great significance for further understanding the auxin regulation. In this study, we biochemically screened the group L of Arabidopsis thaliana glycosyltransferase superfamily for enzymatic activity toward auxins. UGT74D1 was identified to be a novel auxin glycosyltransferase. Through HPLC and LC-MS analysis of reaction products in vitro by testing eight substrates including auxins and other compounds, we found that UGT74D1 had a strong glucosylating activity toward indole-3-butyric acid [IBA], indole-3-propionic acid [IPA], indole-3-acetic acid [IAA] and naphthaleneacetic acid [NAA], catalyzing them to form corresponding glucose esters. Biochemical characterization showed that this enzyme had a maximum activity in HEPES buffer at pH 6.0 and 37°C. In addition, the enzymatic activity analysis of crude protein and the IBA metabolite analysis from transgenic Arabidopsis plants overexpressing UGT74D1 gene were also carried out. Experimental results indicated that over-production of the UGT74D1 in plants indeed led to increased level of the glucose conjugate of IBA. Moreover, UGT74D1 overexpression lines displayed curling leaf phenotype, suggesting a physiological role of UGT74D1 in affecting the activity of auxins. Our current data provide a new target gene for further genetic studies to understand the auxin regulation by glycosylation in plants.
Ectopic Expression of Arabidopsis Glycosyltransferase UGT85A5 Enhances Salt Stress Tolerance in Tobacco
Yan-Guo Sun, Bo Wang, Shang-Hui Jin, Xiao-Xia Qu, Yan-Jie Li, Bing-Kai Hou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0059924
Abstract: Abiotic stresses greatly influence plant growth and productivity. While glycosyltransferases are widely distributed in plant kingdom, their biological roles in response to abiotic stresses are largely unknown. In this study, a novel Arabidopsis glycosyltransferase gene UGT85A5 was identified as significantly induced by salt stress. Ectopic expression of UGT85A5 in tobacco enhanced the salt stress tolerance in the transgenic plants. There were higher seed germination rates, better plant growth and less chlorophyll loss in transgenic lines compared to wild type plants under salt stress. This enhanced tolerance of salt stress was correlated with increased accumulations of proline and soluble sugars, but with decreases in malondialdehyde accumulation and Na+/K+ ratio in UGT85A5-expressing tobacco. Furthermore, during salt stress, expression of several carbohydrate metabolism-related genes including those for sucrose synthase, sucrose-phosphate synthase, hexose transporter and a group2 LEA protein were obviously upregulated in UGT85A5-expressing transgenic plants compared with wild type controls. Thus, these findings suggest a specific protective role of this glycosyltransferase against salt stress and provide a genetic engineering strategy to improve salt tolerance of crops.
Optimization of the Route of Municipal Solid Waste Collection and Transportation
上海浦东新区城市生活垃圾收运路线优化研究

ZHU Ming-hua,FAN Xiu-min,LIU Bing-kai,HE Qi-chang,Alberto Rovetta,
朱明
,范秀敏,刘炳凯,何其昌,Alberto Rovetta

资源科学 , 2009,
Abstract: 城市化的迅猛发展、经济的高速增长,城市垃圾产生量日渐增多,如何实现垃圾减量化、资源化、无害化作为可持续发展领域的焦点,已成为环境综合治理工作中的新难点、新挑战.城市生活垃圾收运费用在垃圾处理系统总费用中占有很大的比例,通过对垃圾收运问题的研究,可以有效节约成本,提高作业效率.文章对城市生活垃圾收运现状进行了分析,以总的垃圾收运距离最短为优化目标建立了城市垃圾收运问题的数学模型,提出了以扫描算法和分枝限界法相结合的求解方法.基于优化的结果和GIS技术实现优化作业路径的动态显示,为实际垃圾收运作业提供决策依据.最后,通过调研区浦东新区的实例表明优化后的作业路径减少了17.7%,节约了垃圾收运的成本,证明了该求解方法的实用性和有效性.
Optical quasi-periodic oscillation and color behavior of blazar PKS 2155-304
Bing-Kai Zhang,Xiao-Yun Zhao,Chun-Xiao Wang,Ben-Zhong Dai
Physics , 2014, DOI: 10.1088/1674-4527/14/8/004
Abstract: PKS 2155-304 is a well studied BL Lac object in the southern sky. The historical optical data during different period have been collected and compiled. Light curves with a time span of 35 years have been constructed. The R-band light curve has been analyzed by means of three methods: epoch folding method, Jurkevich method and discrete correlation function (DCF) method. It is derived that there is an evident periodic component of 317 days (i.e. 0.87 yr) superposed on a long-term trend with large-amplitude variation in the light curve. The variability of this source is accompanied by a slight color variation, and the brightness and color index are correlated with each other. On the long time-scale, PKS 2155-304 exhibits a tendency of bluer-when-brighter, which means the spectrum becomes flatter when the source brightens.
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