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Search Results: 1 - 10 of 27776 matches for " 郝昭程? "
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乙酸对重组大肠杆菌BL21产酶的影响及作用机理研究
戴琨,王腾飞,郝昭,汤丹丹,刘红娟,王瑞明
生物技术通报 , 2015, DOI: 10.13560/j.cnki.biotech.bull.1985.2015.05.032
Abstract: 旨在研究发酵过程中产生的乙酸对重组大肠杆菌BL21(DE3)/pET15b-TreS的生长以及重组海藻糖合酶基因表达的影响,并对其作用机理进行探讨。通过外源添加乙酸(钠)的方法,研究了乙酸钠对重组菌BL21(DE3)/pET15b-TreS生长曲线的影响;利用环境扫描电子显微镜,观察了重组菌形态的变化情况;通过测定菌液的电导率值变化、菌液上清中OD260的变化,研究了乙酸(钠)对菌体细胞膜渗透性、完整性的影响;通过测定菌液上清中β-半乳糖苷酶的活性检测了乙酸钠对菌体细胞内膜的影响;利用荧光分析法检测了乙酸对菌体膜蛋白构象的影响;采用SDS-PAGE电泳研究了乙酸钠对菌体重组海藻糖合酶表达量的影响。结果表明,乙酸(钠)会对重组菌的生长产生一定的抑制作用,可以导致菌体细胞的表面出现凹陷、皱缩;并会影响细胞膜的渗透性、完整性,使得一些细胞内容物发生泄漏;影响细胞膜上的膜蛋白构象,对膜的结构造成一定程度的破坏;对重组海藻糖合酶的表达产生影响。乙酸(钠)对重组菌菌体的生长及重组海藻糖合酶基因的表达有影响,并且菌体细胞膜是其作用的一个靶点。
拟南芥硫酯酶基因在毕赤酵母中的表达
郝昭,王腾飞?,李忠奎?,郝梓凯?,戴琨?,王瑞明?
生物工程学报 , 2015, DOI: 10.13345/j.cjb.140205
Abstract: 硫酯酶在生物体内能催化水解脂酰酰基载体蛋白和饱和脂肪脂酰链,对中链脂肪酸(mediumchainfattyacids,mcfas)的积累起着关键作用。为获得具有生产中链脂肪酸能力的工程菌,以拟南芥cdna为模板,pcr扩增得到其脂酰-acp硫酯酶基因atfata,经ecorⅰ/xbaⅰ双酶切后连接至同样双酶切的质粒中,获得重组质粒ppiczαa-atfat。将重组质粒电击转入毕赤酵母gs115中,通过zeocin抗性筛选,挑选出阳性克隆子并摇瓶发酵诱导,sds-聚丙烯酰胺凝胶电泳分析获得明显目的蛋白条带,首次成功构建atfata的真菌表达系统。气质联用法检测发酵产物胞外游离脂肪酸,发现毕赤酵母atfata重组菌株比起始gs115菌株胞外游离mcfas(主要是辛酸)产量增加51%,mcfas产量占胞外总脂肪酸产量的28.7%,而野生菌中这一比例仅有18.1%,这为日后生产安全无毒害的mcfas探索了一条新的途径。
敲除frdb基因对大肠杆菌厌氧混合酸发酵的影响
郝梓凯,李丕武,郝昭,陈利飞
中国生物工程杂志 , 2014,
Abstract: 以spuec101(产琥珀酸)为出发菌,利用red同源重组技术敲除延胡索酸还原酶基因frdb,得到重组菌株spuec103(△frdb),通过减少延胡索酸生成琥珀酸的通量,实现延胡索酸的积累。实验结果表明:敲除frdb基因后,缺陷菌株生长速率降低,利用葡萄糖的能力也有所降低,同时敲除frdb基因较大程度地改变琥珀酸、延胡索酸等的分布,在两阶段发酵中,当发酵培养基中添加30g/l的葡萄糖时,琥珀酸和延胡索酸得率最高,对比spuec101,spuec103的琥珀酸产量产率由24.6%下降为15.4%,并有延胡索酸和少量的苹果酸生成,分别为0.182±0.002g/l和0.023±0.002g/l,同时丙酮酸和乙酸含量也略有升高,分别由1.87±0.02g/l、0.012±0.002g/l上升到2.36±0.03g/l、0.862±0.012g/l。
THE DYNAMIC MECHANISM CALCULATION AND ANALYSIS OF FOUR LEVEL AVALANCHE UPCONVERSION SYSTEM
四能级动态雪崩上转换过程的计算分析

Hao Zhao,Chen Xiaobo,Zhang Guangyin,Li Meixian,Mao Yuhong,
郝昭
,冯志萍

红外与毫米波学报 , 1997,
Abstract: Properties of the dynamic mechanism of a four level avalanche upconversion system were investigated by using rate equations.
栀子苷和栀子黄色素改善睡眠作用的研究
郝昭,江璐,车会莲,石宝霞,李博,何计国
食品科学 , 2009,
Abstract: ?目的:探寻栀子中具有改善睡眠作用的活性成分。方法:健康icr小鼠,分设栀子苷低剂量组(65mg/kgbw)、栀子苷中剂量组(650mg/kgbw)、栀子苷高剂量组(1950mg/kgbw),栀子黄色素低剂量组(30mg/kgbw)、栀子黄色素中剂量组(300mg/kgbw)、栀子黄色素高剂量组(900mg/kgbw)和空白对照组(蒸馏水),连续灌胃28d后,测定各项睡眠指标。结果:与对照组比较,栀子黄色素低、中、高剂量组分别将小鼠睡眠潜伏期缩短了18.2%(p<0.05)、21.4%(p<0.01)、26.2%(p<0.01);同时,将小鼠睡眠时间分别延长了32.4%(p<0.05)、48.1%(p<0.01)、52.4%(p<0.01)。枙子苷对睡眠无明显的改善作用。结论:栀子黄色素具有改善睡眠作用。
莫迪亚诺《暗店街》与王小波《万寿寺》的失忆母题
The Motive of Memory Loss in Patrizio Modiano’s Missing Person and Wang Xiaobo’s Wanshou Temple
 [PDF]


World Literature Studies (WLS) , 2015, DOI: 10.12677/WLS.2015.31001
Abstract:
《暗店街》与《万寿寺》均从“失忆”主题出发,探索人的个体本质与生存状态,具有共同的出发点和目的性;与此同时,两者又因各自不同的文化传统和时代背景,呈现出对个体存在的不同的探索方式和探索结果。《暗店街》和《万寿寺》可以说是对历史伤痕的一种写作,但在这种伤痕写作中,体现出不同的个体生存状态。《暗店街》叙述个人对个体经历的回忆以及个体存在的虚无感,《万寿寺》描写个人对集体记忆的反思以及个人在群体生存中的呻吟。《暗店街》与《万寿寺》以回忆的艺术,唤醒了人们对本体的认识和对生存状态的反思。
Both telling stories of memory loss, Missing Person and Wanshou Temple study the state of being and inner characters of individuals for the same purpose; however, the two works take on different study methods and conclusions grounded in different cultural traditions and times. Missing Person and Wanshou Temple can be considered as description of the scars in history, during the process of which, individuals’ state of being is fully presented. Missing Person describes the recall of individual experience and the lacking sense of existence, while Wanshou Temple presents the introspection on memories shared by group from individual perspectives, as well as their sorrows of the life in group. The two works call for recognition on inner characters and provoke introspection on state of being.
关于马氏环境中马氏链的一个极限性质
A Limit Property for Non-Homogeneous Markov Chain in Markov Environments
 [PDF]


Pure Mathematics (PM) , 2016, DOI: 10.12677/PM.2016.61012
Abstract:
本文研究马氏环境中非齐次马氏链泛函的滑动平均强极限性质。通过构造一列带参数且期望为1的随机变量,利用Borel-Cantelli引理来研究随机环境中马氏链的强极限定理,得到了马氏环境中马氏链泛函滑动平均的一个强极限定理,推广了若干已有结论。
In this paper we discuss the strong limit properties for moving average of functions of non-ho- mogeneous Markov chain in Markov environments. By constructing a sequence of random va-riables with one parameter and take 1 as the expectation, with the aid of the classic Borel-Cantelli lemma, we study the strong limit properties of Markov chain in random environments and obtain a strong limit theorem for moving average of functions of non-homogeneous Markov chain in Markov environments. Moreover, we generalize the existing results.
斯图亚特•霍尔:表征的运作
Study on the Cultural Representation Theory and Signifying Practices of Stuart Hall
 [PDF]


World Literature Studies (WLS) , 2016, DOI: 10.12677/WLS.2016.42007
Abstract:
斯图亚特?霍尔被喻为“文化研究之父”,他所建构的“文化表征”理论对20世纪后半叶以来的文化研究理论和后殖民主义理论的发展做出了杰出的贡献,将结构主义语言学、符号学以及福柯权力话语理论融入表征概念之中,形成独特的文化表征理论,对“文化”的重新定义。同时,以表征为内核思考其在各种文化现象中的运作方式以及发挥的作用,不断地反思现代社会留给我们的理论遗产。
Stuart Hall, known as the Father of Cultural Studies, has constructed the theory of cultural representations and signifying practices, which contributed greatly to the theories of Cultural Studies and Postcolonial Studies since the second half of the 20th century. Stuart Hall integrated the theory of structural linguistics, semiotics and Foucault’s power discourse theory into the concept of representation, and formed a unique cultural representation theory, which is a new definition of “culture”. Simultaneously, taking the Cultural Representations as the center, Stuart Hall researches the operation mode and impact of culture in sorts of cultural phenomena, and reflects the theoretical heritages from the modern society.
BLUE UPCONVERSION EMISSION IN Yb,Tm-DOPEDPENTAPHOSPHATE GLASSES
Tm,Yb掺杂五磷酸盐非晶中的上转换蓝光发射

HAO ZHAO,CHEN XIAO-BO,HOU YAN-BING,SONG FENG,WANG HONG,ZHANG GUANG-YIN,
郝昭
,陈晓波,侯延冰,宋峰,王虹,张光寅

物理学报 , 1997,
Abstract: Much stronger blue emission have been observed in phosphate non-crystals at room temperature,co-doped with Yb3+ and Tm3+ when excited into the Yb3+ 2F7/2 state and the excited states of Tm3+ at 966nm by laser diode.Energy transfer and back transfer between the two rare earth ions,participation of phonons were demonstrated to be responsible for the upconversion process.
历史制度主义视角下区域文化资源产业化政策供给体系研究——以徐州汉文化资源为例
The Research on Policy Supplies System of Regional Culture Resources Industrial Production from the Angle of View of Historical Institutionalism—Take the Xuzhou Han Culture Resources as an Example
 [PDF]

朱锦
Modern Management (MM) , 2012, DOI: 10.12677/mm.2012.21003
Abstract: 江苏拥有丰富、优良的历史文化资源,这是优势文化资源向文化资本转化的产业基础之一,其中尤以徐州市汉文化资源为代表。本文认为,充分利用汉文化资源的产业化资源优势,建立相关的政策供给体系,有助于汉文化资源的合理开发,并且为区域性文化资源的产业开发具有较好的借鉴意义。
Jiangsu has rich and fine historical culture resources which are one of the industrial foundations that the advantageous culture resources which are transformed into the cultural capital, as represented by Xuzhou Han culture resources especially. This article establishes the related policy supplies system through the advantages of industrial development of the Han culture resources, which will be helpful in reasonable development of Han culture resources and be good for industrial development of regional culture resources.
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