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Search Results: 1 - 10 of 32250 matches for " 邓优锦 "
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74株草菇遗传多样性分析
江玉姬,,刘新锐,谢宝贵
核农学报 , -1, DOI: 10.11869/j.issn.100-8551.2015.11.2084
Abstract: 为了保护和充分利用草菇遗传资源,利用RAPD、ISSR、SRAP3种分子标记对来源于不同地区的74个草菇菌株进行遗传多样性分析,并根据3种分子标记建立综合聚类图。结果表明:当相异系数D=0.28时,可将74个草菇菌株分为16个类群,其中有9个菌株各自为一类,其它类群包括多个菌株,最大的一个类群包括32个菌株。供试的74个菌株间的相异系数范围从0~0.79,具有较丰富的遗传多态性。74个菌株中只有V0020(26号)、V0059(71号)、V0060(72号)3个菌株的相异系数为0,而其它的菌株均有一定的差异性。3种分子标记各自的分析结果有差异,因此单独应用一种分子标记对种质资源进行遗传多样性分析,具有较大的局限性。本文综合运用RAPD、ISSR、SRAP3种分子标记,结果此3种分子标记单独分析更可靠。本研究可为其它物种间的遗传资源多样性分析提供新方法。
早熟金针菇新品种‘农金6号’
刘新锐,谢宝贵*,江玉姬,
园艺学报 , 2014,
Abstract: 金针菇‘农金6号’是以黄色品种‘三明一号’与白色品种‘金21’、‘金3’为亲本,采用三亲本杂交选育而来,具有菇形好,生育期短,产量高,易管理,适应性广的突出特点。
双向核迁移在香菇遗传和育种中的应用研究
刘靖宇,刘新锐,,刘芳,谢宝贵
菌物学报 , 2011,
Abstract: 在来源于香菇Lentinulaedodes不同双核菌株的单核菌株间进行单-单杂交,并利用双向核迁移现象获得两对双核菌株。之后采用挑取菌落尖端单根菌丝的方法纯化所获得的双核菌株并通过双-单杂交进行单向核迁移,进一步纯化所获得的双核菌株。从菌落形态、显微镜镜检、出菇实验和分子标记等4个方面对两对配对单核和所获得的双核菌株进行分析和鉴定。结果表明:(1)通过双向核迁移的方法可以获得具有两个相同细胞核和不同受体细胞质的香菇双核菌株;(2)选择不同的受体细胞会对双核菌丝的菌落形态和出菇期产生明显的影响,表明香菇的
四种重金属在金针菇栽培过程中的迁移规律
江玉姬,黎志银,谢宝贵,,刘新锐,肖淑霞
菌物学报 , 2014, DOI: 10.13346/j.mycosystema.130289
Abstract: 为了掌握铅、镉、砷、汞4种有害重金属元素在金针菇栽培过程中的富集和迁移,为金针菇产品质量控制提供依据。采用在培养料中添加一定量的铅、镉、汞、砷栽培金针菇,测定其在各栽培阶段培养料、金针菇子实体中的含量。结果表明,在试验的浓度范围内,金针菇子实体中4种重金属的含量随着培养料中添加量的增加而增加,说明金针菇子实体中4种重金属主要是来源于培养料,但金针菇子实体对不同重金属的吸收富集能力不同,对汞的吸收富集能力最强,富集系数最高达到7.590,富集能力大小依次为汞>镉>砷>铅。
草菇磷酸果糖激酶(PFK)基因克隆、结构及不同菌株中表达量分析
刘朋虎,谢宝贵,,江玉姬
菌物学报 , 2013,
Abstract: 从福建主栽品种屏优一号PY分离到2个不能正常出菇的单孢菌株PYd21、PYd15,二者配对杂交得到可正常出菇异核菌株H15-21。用solexa测序技术对PYd21基因组从头测序(denovosequencing),对PYd15、PYd21、H15-21的菌丝体分别进行数字基因表达谱测序,对8个样品(PYd15、PYd21、H15-21的菌丝体,H15-21出菇后的原基、钮扣期菌柄、蛋形期菌柄、伸长期菌柄和成熟期菌柄)mRNA等量混合物进行转录组测序。克隆测序了PYd21与PYd15中磷酸果糖激酶(PFK)基因,比对结果表明二者序列一致。利用转录组数据对PFK基因结构进行分析,结果表明:该基因全长3,494bp,有12个外显子、11个内含子;开放阅读框(ORF)全长2,457bp,编码818个氨基酸,5′端非翻译区(5′UTR)长度281bp,3′端非翻译区(3′UTR)全长103bp;RNA在加工过程中存在1种可变剪切类型、6个可变剪切位点。数字基因表达谱分析结果表明,PFK基因在PYd21、PYd15及H15-21中的标准表达量分别为71.08、120.61、251.85(transcriptpermillioncleantags,TPM),表达量依次升高,与菌丝生长速度显著正相关。并且异核菌株H15-21表达量高于2个单孢菌株之和,基因表达存在协同增效作用。采用实时荧光定量PCR技术对基因表达量进行验证,表达谱数据切实可靠。
57株毛头鬼伞遗传多样性分析
江玉姬,谢宝贵,,刘新锐,江洪
菌物学报 , 2013,
Abstract: 运用SRAP、RAPD、ISSR3种分子标记技术对来源不同地区的57株毛头鬼伞Coprinuscomatus进行了遗传多样性分析,通过3种分子标记进行聚类分析,当相异系数D为0.48时,可以把57株毛头鬼伞分为4类:Ⅰ类包括Co0001;Ⅱ类包括Co0003;Ⅲ类包括Co0005;Ⅳ类包括其余54个菌种。供试的57个菌株间的相异系数范围从0–0.72,具有一定的遗传多态性。但其中有许多菌株两者之间的相异系数为0,说明毛头鬼伞菌种存在着比较严重的同种异名现象。
高通量测序检测金针菇RNAi转化子插入位点及拷贝数
丑天胜,王威,施乐乐,卢园萍,,谢宝贵
菌物学报 , 2015, DOI: 10.13346/j.mycosystema.150089
Abstract: 本研究对金针菇Flammulinavelutipes的一个RNAi转化子菌株1382R3进行了高通量测序,以本实验室先前获得的野生型W23基因组数据为参考,分析了该转化子的基因插入位点以及拷贝数。转化子菌株1382R3是通过农杆菌介导将fv-hmg1-RNAi载体转化至金针菇菌株并通过PCR检测筛选标记而得到。通过BLAST将转化子测序的reads对外源载体和基因组定位,找到具有基因组序列(GS)和外源载体序列(ES)两种序列的临界reads,并据此使用PERL语言程序成功在转化子1382R3菌株中找到两个插入位点。对两个插入位置的序列分析表明:在插入位点1,T-DNA片段部分插入;在插入位点2,T-DNA全部插入到基因组。两个插入位点都对基因组内源基因的表达造成了一定的干扰。此方法拓宽了高通量测序技术的应用范围,将其运用到遗传转化插入位置和拷贝数的研究中,有利于食用菌的功能基因组及基因工程研究。
Cloning, structural analyses and expression levels of phosphofructokinase gene in different strains of Volvariella volvacea
草菇磷酸果糖激酶(PFK)基因克隆、结构及不同菌株中表达量分析

LIU Peng-Hu,XIE Bao-Gui,DENG You-Jin,JIANG Yu-Ji,
刘朋虎
,谢宝贵,,江玉姬

菌物学报 , 2013,
Abstract:
Comparative analysis of proteomic profile at different development stages of Volvariella volvacea by iTRAQ-coupled 2D LC-MSMS
iTRAQ结合2D LC-MS/MS技术在草菇不同生长发育时期蛋白质组分析中的应用

LIU Jing-Yu,JIANG Yu-Ji,XIE Bao-Gui,CHEN Bing-Zhi,LIAO Wei,DENG You-Jin,
刘靖宇
,江玉姬,谢宝贵,陈炳智,廖伟,

微生物学通报 , 2012,
Abstract: Objective] This work aims to investigate differential expression proteins at different development stages of Volvariella volvacea using isobaric tags for relative and absolute quantification (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) proteomics approach. Methods] Firstly, the proteins extracted from V. volvacea at different development stages were analyzed by SDS-PAGE. Secondly, the tandem mass spcetrometry data obtained from 2D LC-MS/MS were used to search using MASCOT search engine. After that, principal component analysis, hierarchical clustering and Gene Ontology were used to analyze the detected results. Results] Results showed that 1 039 protein groups, included a total of 2 335 unique peptides, were identified. Among them, 1 030 protein groups were provided quantitative information. Contrast to mycelia, 64 up-regulated and 150 down-regulated significantly differential proteins were found in fruiting bodies at different development stages of V. volvacea. Bioinformatics analysis revealed that iTRAQ-coupled 2D LC-MS/MS was a unique method for isolating and identifying protein groups of V. volvacea at different development stages. Conclusion] It is helpful to insight into the molecular mechanism of the fruiting body formation and development of V. volvacea and other macro-basidiomycetes in the future.
Isolation and identification of a bacterial strain JS018 capable of degrading several kinds of organophosphate pesticides
一株能高效降解几种有机磷农药的菌株JS018的鉴定

JIANG Yu-ji,DENG You-jin,LIU Xin-rui,XIE Bao-gui,HU Fang-ping,
江玉姬
,,刘新锐,谢宝贵,胡方平

微生物学报 , 2006,
Abstract: Organophosphate pesticides are used widely all over the world and play an important role in plant pest control. However these pesticides are considered as pollutants and harmful to human health. To search for microorganisms that can degrade organophosphate pesticides with high efficiency, a bacterial strain, coded as JS018, was isolated and screened from the soil in the vicinity of Shanming Pesticides Factory, Shanming, Fujian. Laboratory tests showed that the bacterium could degrade several kinds of organophosphate pesticides, such as Parathion-methyl and phoxin. The strain's degrading rates on phoxin, Parathion-methyl, hostathion and dichlorvos in LB liquid fermentation medium for 36 h were 99%, 96%, 80.4% and 69.0% respectively. The bacterial colonies on LB plate appeared shiny and pale-pink in color. The bacteria were Gram-negative coccoids, 0.5 - 0.7 microm in diameter. They grew well at 30 - 38 degrees C and pH 7.0 - 9.0. The optimal temperature and pH for cell growth was 32 degrees C and pH 7.5 - 8.0, respectively. They did not grow in medium containing 6% or more NaCl. The antibiotic susceptibility tests showed that the strain was resistant to ampicillin, penicillin and lincomycin. It was sensitive to kanamycin, tetracycline and gentamicin. Laboratory tests also showed that the strain could ferment D-glucose, trehalose, melezitose and ethanol. It was negative in the production of indole and hydrogen sulfide. It could not liquefy gelatin, utilize citrate, nor ferment L-arabinose, sucrose, D-mannitol, D-xylose, fructose, D-galactose, maltose or lactose. The catalase, urease and nitrate reduction were positive. Based on its morphological, physiological and biochemical properties as well as the 16S rDNA sequence analysis result, the strain was tentatively identified as Roseomonas sp.
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