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Search Results: 1 - 10 of 80981 matches for " 蔡海波 "
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影响脐血造血细胞体外培养与检测的因素
张孝兵,海波
华东理工大学学报 , 1999,
Abstract: 考察了分离方法、血浆(血清)、渗透压、细胞因子的保存等因素对脐血造血细胞体外培养和检测的影响。结果表明,用明胶沉降加密度梯度离心分离两步法分离比传统方法能分离到更多的单个核细胞;混合脐血血浆比胎牛血清更能支持造血细胞的生长;高渗透压对集落形成有一定影响,而低渗透压对集落形成更为有害;在37℃下保存细胞因子,其生物活性下降最快,-30℃冷冻保藏较好地保持其活性,解冻次数对生物活性影响不显著。
基于直射线近似的一步法基准面校正方法研究
杨锴,杰雄,范旭,海波
地球物理学报 , 2009, DOI: 10.3969/j.issn.0001-5733.2009.04.025
Abstract: 地形基准面校正算子(TopographicDatumingOperator,以下简称TDO)是一种基于直射线近似得到的基准面延拓算子.TDO可以视为是两步法波动方程基准面校正与常规静校正之间的一种过渡算法,该方法的最大特点在于它可以基于共炮点道集将炮点和检波点同时延拓到给定的水平基准面,因此相对于常规的两步法叠前波动方程基准面校正,TDO方法可以认为是一种更为高效的一步法基准面延拓方法.本文基于理论与实际数据论证了上述观点.
既有基坑延深开挖稳定性评价与支护方案确定
海波,吴顺川,周喻
岩土力学 , 2011,
Abstract: 以北京市某特殊基坑工程为背景,采用flac3d软件,选用mohr-coulomb准则,对基坑在现有支护方案条件下进行延深开挖,计算分析桩锚支护体系的变形、受力情况。结果表明,基坑在现有支护方案条件下进行延深开挖将导致护坡桩抗弯性能不足、支护结构水平位移过大、锚固体系失效等问题,要保证基坑延深开挖后的安全稳定,必须对护坡桩增设支点,增加支护力。根据设计变更要求及施工现场实际条件,对多种加固支护方案进行比选,最终选用对护坡桩增设锚索的方案进行延深基坑的预加固。采用数值方法模拟分析上述加固方案后的基坑延深开挖力学行为,预测基坑及支护结构的变形情况,并与现场监测数据进行对比分析。结果表明,基坑延深开挖预加固支护方案合理有效,表明该方法对于类似工程的设计优化及现场施工具有重要的参考意义。
移动自组网基于邻居变化率稳定路径选择方法
一兵?,海波,李忠诚?,谢高岗?
软件学报 , 2007,
Abstract: 节点移动是导致移动自组织网络性能下降、限制网络规模扩展的关键因素之一.寻找稳定路径是减小节点移动影响的有效手段.现有的稳定路径寻找方法存在以下局限:需要节点具有地理位置定位的硬件功能支持,或需要信号强度上传的交叉层功能支持.为此,提出了不需要特殊硬件支持、可独立于底层协议工作、基于邻居变化率的稳定路径选择方法.以aodv(adhocon-demanddistancevector)按需路由协议为基础,扩展为ncr-aodv(neighborchangeratioadhocon-demand
一种新型生物反应器在造血干/祖细胞体外扩增中的应用
周美琴?,海波,谭文松?
生物工程学报 , 2008,
Abstract: 针对造血干/祖细胞体外扩增对培养环境的需求,结合静/动态培养的特点,开发了一种新型的生物反应器用于造血干/祖细胞的体外扩增。在该生物反应器内,采用scf+tpo+flt-3细胞因子组合,比较了静态和循环培养两种方式体外扩增脐血cd34+细胞的效果。培养7d后,总细胞分别扩增了(13.86±4.26)和(7.23±2.67)倍,显示静态培养有利于总细胞的扩增;cd34+细胞扩增倍数、培养物中cd34+细胞含量均相近,无显著性差异;而cd34+cd38-细胞扩增倍数以及培养物中cd34+cd38?细胞的百分含量分别为(1.82±0.58)和(3.90±0.85)倍以及(9.45±4.85)和(37.47±14.06)%,循环培养明显高于静态培养。可见,在该生物反应器内,采用静态和循环两种培养方式,均能实现造血干/祖细胞的体外扩增,但静态培养促使造血干细胞向定向祖细胞分化,而循环培养则更有利于早期造血干细胞的扩增。
基于中心组合设计和响应面分析的血清替代物浓度优化 Optimization of Serum Substitute Concentration in Serum-Free Media for CIK Cells Using Central Composite Design and Response Surface Analysis
陈小东,海波,谭文松
- , 2016,
Abstract: 细胞因子诱导杀伤细胞(Cytokine-induced killer cells,CIK cells)是一类广泛应用的肿瘤过继免疫疗法的效应细胞,其体外扩增过程中常需要使用无血清培养基。今以胰岛素、亚麻酸、胆固醇和乙醇胺4种可替代血清促进细胞扩增的组份为研究对象,采用中心组合设计和响应面分析相结合的方法,考察上述组分的浓度以及相互作用对单个核细胞扩增的影响,得到了可支持单个核细胞扩增的最优浓度,分别为10、5.66、19.86和1.22 mg·L-1。将上述4种组分以优化的浓度添加到DMEM/F12和IMDM以1:1体积比混合而成的基础培养基中,制成无血清培养基Optimizer。以含10% FBS的RPMI 1640为对照,采用半量稀释法培养脐血单个核细胞,以培养14天的总细胞扩增倍数、细胞组成、CD3+CD56+细胞扩增倍数以及对K562细胞的杀伤活性为指标,评价了制成的无血清培养基Optimizer支持CIK细胞扩增的效果。结果显示,采用所制备的无血清培养基Optimizer,总细胞扩增倍数为56.54±18.87,明显高于SFM1的5.14±1.03(p<0.05)和SFM2的3.59±0.56(p<0.05),与含10% FBS的RPMI 1640的35.24±20.92(p>0.05)接近;CD3+细胞为97.98%±1.41%,与含10% FBS的RPM I1640的94.34%±1.29%相当(p>0.05);尽管CD3+CD56+细胞比例18.17%±7.38%低于含10% FBS的RPMI 1640中的数值25.49%±3.35%(p<0.05),但两种培养基的CD3+CD56+细胞扩增倍数分别为440.86±222.89和429.27±249.16,没有显著性差异(p>0.05);当效靶分别为9:1时分别采用两种培养基扩增的细胞对K562细胞的杀伤活性均能达到50%以上。该研究结果为用于CIK细胞体外扩增的无血清培养基的开发提供了依据。
川芎提取物穴位敷贴对大鼠脑缺血再灌注损伤的保护作用
杨洁红,海波,,
中国中医药信息杂志 , 2004,
Abstract: 目的观察川芎提取物穴位敷贴对大鼠脑缺血再灌注损伤的保护作用。方法采用四血管阻断法(4vo法)制作大鼠脑缺血再灌注损伤模型,测定丙二醛(mda)、超氧化物歧化酶(sod)活性和6-酮-前列腺素flα(6-keto-pgflα)含量。结果川芎提取物穴位敷贴可使脑组织mda含量显著减少,使6-keto-pgflα含量显著升高。结论川芎提取物穴位敷贴具有抗自由基损伤、抗凝血作用,对脑缺血再灌注损伤具有显著保护作用。
FL对脐血造血细胞长期液体培养的影响*
张孝兵,海波,赵佼,谭文松,俞俊棠
生物工程学报 , 1999,
Abstract: 用脐血进行千细胞移植有许多优点,但有一个主要的缺点是可获得的细胞数量有限。因此脐血干细胞的体外扩增对于其临床应用具有重要意义。考察了Flt-3配体(FL)和干细胞因子(SCF)、白介索3(IL一3)、IL-6、粒细胞集落刺激因子(G-csF)、粒细胞巨噬细胞集落刺激因子(GM—CSF)的组合对脐血细胞扩增和分化的影响。培养42d,总细胞最多扩增了385,30±163 51倍(FL+SCF+G.CSF+GM—CSF),粒细胞巨噬细胞集落形成单位(CFU-GM)在第28天达到最高,最高扩增了409.52±189.50倍(FL十SCF+IL-3+IL一6)。FL与SCF等细胞因子具有协同作用,对所有考察的细胞因子组合中,加入FL都使总细胞和CFUGM的扩增倍数增加。FL+SCF培养的总细胞扩增最小,而CFU-GM长时间保持在较高水平,表明这FL和SCF有利于保持造血干细胞的活性,防止细胞分化。在存在G-CSF和GMCSF的培养中,总细胞获得了最大的扩增,但CFU-GM达到最大后很快下降至O,表明G-CSF和GM—CSF促进了细胞的分化。结果提示,细胞因子组合对脐血造血细胞的扩增和分化具有重要的作用.FL和SCF可促进造血细胞的扩增,而G-CSF和GM—CSF等可导致细胞的过度分化。
The Application of a New-type Bioreactor in the ex vivo Expansion of Hematopoietic Stem/Progenitor Cells
一种新型生物反应器在造血干/祖细胞体外扩增中的应用

Meiqin Zhou,Haibo Cai,Wensong Tan,
周美琴
,海波,谭文松

生物工程学报 , 2008,
Abstract: Based on the requirement of culture conditions for hematopoietic stem and progenitor cells (HSPCs) ex vivo expansion, we developed a new-type bioreactor by combining superiorities of static and stirred culture models. Stem cell factor(SCF), thrombopoietin(TPO), FLT-3 ligand(Flt-3) were used as the cytokines cocktails. The effects of the static and cyclic culture on the expansion characteristics of CD34+ selected cells were compared in the new-type bioreactor. After 7 d cultures, the expansion of total cells in the static culture was 13.86 ± 4.26 fold, higher than that in the cyclic culture (7.23 ± 2.67 fold). The analysis of the fold expansion and the proportion of CD34+ cells showed that there was no marked difference between the static culture and the cyclic culture. However, the fold expansion and the proportion of CD34+CD38- cells were higher in the cyclic culture than those in the static culture (3.90 ± 0.85 versus 1.82 ± 0.58), which reflected more primary CD34+CD38- cells were obtained in the cyclic culture. The above results demonstrated that both the static culture and the cyclic culture could be used in ex vivo expansion of CD34+ cells with the new-type bioreactor. In static culture hematopoietic stem cells differentiated into progenitor cells, whilst the cyclic culture favored the expansion of primary HSPCs.
The Application of a New-type Bioreactor in the ex vivo Expansion of Hematopoietic Stem/Progenitor Cells
一种新型生物反应器在造血干/祖细胞体外扩增中的应用

Meiqin Zhou,Haibo Cai,Wensong Tan,
周美琴
,海波,谭文松

微生物学报 , 2008,
Abstract: Based on the requirement of culture conditions for hematopoietic stem and progenitor cells (HSPCs) ex vivo expansion, we developed a new-type bioreactor by combining superiorities of static and stirred culture models. Stem cell factor(SCF), thrombopoietin(TPO), FLT-3 ligand(Flt-3) were used as the cytokines cocktails. The effects of the static and cyclic culture on the expansion characteristics of CD34+ selected cells were compared in the new-type bioreactor. After 7 d cultures, the expansion of total cells in the static culture was 13.86 ± 4.26 fold, higher than that in the cyclic culture (7.23 ± 2.67 fold). The analysis of the fold expansion and the proportion of CD34+ cells showed that there was no marked difference between the static culture and the cyclic culture. However, the fold expansion and the proportion of CD34+CD38- cells were higher in the cyclic culture than those in the static culture (3.90 ± 0.85 versus 1.82 ± 0.58), which reflected more primary CD34+CD38- cells were obtained in the cyclic culture. The above results demonstrated that both the static culture and the cyclic culture could be used in ex vivo expansion of CD34+ cells with the new-type bioreactor. In static culture hematopoietic stem cells differentiated into progenitor cells, whilst the cyclic culture favored the expansion of primary HSPCs.
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