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Search Results: 1 - 10 of 113953 matches for " 范晓枫 "
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张、压应力刺激下人牙周膜成纤维细胞早期增殖活性和差异表达基因的变化
,王羽,李宇,赵志河
华西口腔医学杂志 , 2012, DOI: 10.3969/j.issn.1000-1182.2012.05.004
Abstract: 目的对比在张应力和压应力两种不同性质力的刺激下人牙周膜成纤维细胞(HPDLF)增殖活性的变化,并对差异表达基因进行筛选,探讨不同应力作用后HPDLF增殖变化的分子机理。方法使用四点弯曲加力装置对细胞进行0.5Hz、4000μstrain的应力加载,加载时间2h,采用流式细胞术测定张、压应力刺激对HPDLF增殖活性的影响,应用基因芯片技术检测两种应力刺激下细胞的差异表达基因。结果在两种应力刺激作用下,S期细胞百分比和细胞增殖指数均降低;差异表达的基因最主要位于细胞核,集中在转录因子活性相关基因群,参与最多的生物过程为转录因子调控,其中压应力引起的差异表达基因和生物过程的数量较张应力更多。结论1)周期性张、压应力作用下,HPDLF增殖变缓、细胞周期阻滞。2)细胞增殖变缓和细胞周期阻滞可看成是细胞对外界刺激的适应和自我保护,有利于HPDLF有更多的时间决定如何应答外界应力刺激,其应答的结果首先是转录水平上基因表达的改变,最主要的生物学反应是核转录调控。3)HPDLF对周期性压应力更敏感。
活动式前牵引矫治器口内固位装置改进的临床研究
张慧,刘钧,,赵青,张景慧,赵志河
华西口腔医学杂志 , 2008,
Abstract: 目的探讨活动式前牵引矫治器口内固位装置改进后治疗骨性Ⅲ类错的临床效果。方法选择18例需行前牵引的替牙期骨性Ⅲ类错患者为研究对象,将18例患者随机分为传统组(T组)和改良组(M组),每组9例。根据分组,固位方式分别选择传统型活动式前牵引矫治器和改良型活动式前牵引矫治器。改良型活动式前牵引矫治器在传统型矫治器基础上,在上颌中切牙间加做箭头卡固位。分别在患者初次佩戴和第1次复诊时使用2个相同的弹簧测力计测定2种前牵引矫治器的最小脱位力,并在矫治初始3个月记录每次复诊时患者的牵引后脱位情况。结果初次佩戴和第1次复诊时M组与T组的最小脱位力之间均有统计学差异(P<0.01,P<0.001),改良型活动式前牵引矫治器的最小脱位力明显大于传统型活动式前牵引矫治器。矫治初始3个月内M组脱位2次,T组脱位9次,两组之间有统计学差异(P<0.05)。结论在传统型活动式前牵引矫治器上加做上颌中切牙间箭头卡,可以有效避免牵引加力过程中矫治器向下脱位,提高了前牵引矫治器的治疗效果。
社交网站中的自我呈现对生活满意度的影响:积极情绪和社会支持的作用
The Impact of Self-presentation in Online Social Network Sites on Life Satisfaction: The Effect of Positive Affect and Social Support

牛更,鲍娜,周宗奎,翠英,孔繁昌,
- , 2015, DOI: 10.16187/j.cnki.issn1001-4918.2015.05.07
Abstract: 采用社交网站中的积极自我呈现和真实自我呈现问卷、积极情绪量表、领悟社会支持量表和生活满意度问卷对武汉某高校645名在校大学生进行调查,探讨了社交网站中的积极自我呈现、真实自我呈现对个体生活满意的影响以及积极情绪和社会支持在其中的中介作用,结果发现:(1)社交网站中的积极自我呈现和真实自我呈现与积极情绪、社会支持和生活满意度都呈显著正相关;(2)真实和积极自我呈现对生活满意度都有显著的直接预测作用;此外,真实自我呈现还可以通过社会支持的中介作用对生活满意度产生影响,而积极自我呈现不仅可以通过积极情绪的单独中介作用对生活满意度产生影响,还能通过积极情绪—社会支持的链式中介作用对生活满意度产生影响。
In order to investigate the impact of self-presentation in SNS (Social Network Site)on life satisfaction as well as the mediation of positive affect and social support, a sample of 645 undergraduate students completed Questionnaire of Self-presentation in SNS, Positive Affect Scale, Perceived Social Support Scale and Satisfaction With Life Scale. Results revealed that: (1)Both positive and honest self-presentation in SNS were positively correlated with positive affect, social support and life satisfaction; (2)Both honest and positive self-presentation could predict life satisfaction directly; and honest self-presentation also had an indirect effect on life satisfaction through the mediation of social support, while positive self-presentation could affect life satisfaction through the mediation of positive affect as well as the serial mediation of positive affect and social support.
网络相关文字刺激和压力对网络成瘾者线索诱发渴求的影响
The Impact of Internet-related Word Stimulus and Stress on Internet Addicts' Cue-induced Craving

牛更,,周宗奎,孔繁昌,翠英,魏华
- , 2016, DOI: 10.16187/j.cnki.issn1001-4918.2016.04.14
Abstract: 渴求感特别是线索诱发渴求是成瘾行为维持和戒断失败的主要原因。本研究通过两个实验分别探讨了网络相关文字刺激和压力对网络成瘾者线索诱发渴求的影响。研究一以25名网络成瘾大学生为被试,以网络相关和中性词语为实验材料,采用线索暴露范式(Cue-reactivity Paradigm)的研究发现,只有网络相关文字刺激能显著诱发网络成瘾者的渴求感。在此基础上,研究二以40名网络成瘾大学生为被试,以公开演讲范式为压力诱发情境,以网络相关词语和中性词语为实验材料,采用线索暴露范式(Cue-reactivity Paradigm)的研究发现,在压力条件下,网络相关文字能诱发网络成瘾者更为强烈的渴求感。本研究深化了对网络成瘾形成维持心理机制的研究,也为网络成瘾的干预矫正提供了思路。
Craving, especially the cue-induced craving not only maintains and exacerbates the addictive behaviors, but also often leads to relapse. Based on existed researches, this study aimed to investigate the effect of Internet-related word stimulus and stress on Internet addicts' cue-induced craving through two experiments. In the first experiment, 25 Internet addicts participated in the Cue-reactivity experiment with Internet-related words and neutral words. The results showed that, only Internet-related words could significantly arouse Internet addicts' cue-induced craving. And in the second experiment, we examined the effect of stress on Internet addicts' cue-induced craving among 40 Internet addicts. Participants' stress was induced by Public speaking paradigm. The results showed that, under the condition of stress, Internet-related words could arouse more intense carving for Internet among Internet addicts. This study might deepen our understanding of the formation and maintenance mechanism of Internet addiction. Also, it provides a way for the intervention of Internet addiction from the perspective of craving.
抗cd20嵌合抗体片段fab′突变体的表达和活性研究
刘银星 熊冬生 冬梅 邵 许元富 朱祯平 杨纯正*
生物工程学报 , 2003,
Abstract: 利用pcr方法从抗cd20单链抗体(scfv)表达载体上扩增抗cd20抗体轻链可变区基因(vl)、重链可变区基因(vh),同时在抗体的可变区引入突变,然后将vh、vl基因重组到fab′表达载体pyzf1中,构建抗cd20嵌合抗体fab′片段表达载体,并在大肠杆菌16c9中进行高效可溶性分泌表达。经大量的筛选,获得一个产量和活性均有所提高的突变克隆。其突变位点在轻链可变区的cdr1区,即g77→a(ser→asn)。突变的抗体的表达量为每克干菌3.8mg,而未突变抗体的表达量为每克干菌1.3mg。突变体的亲和力常数ka为2.2×109l/mol,约为突变前的2倍。竞争性免疫荧光抑制实验表明,突变的fab′片段能竞争性抑制鼠源性抗cd20抗体hi47和cd20表达细胞raji细胞的结合,使hi47的结合阳性率由98%下降至37.55%,体外细胞生长抑制试验亦证明突变的fab′片段的抑制活性明显高于未突变的抗体。
131例纵隔原发性神经源性肿瘤手术治疗的临床经验分析
方硕,詹成,张毅,姚光宇,,张永星△,
- , 2017,
Abstract:
从葵花籽提取蛋白的工艺及其产品

食品科学 , 1985,
Abstract: ?向日葵是现在增长速度最快的一种大面积种植油料作物,它已经成为世界上食用油的第二大来源,饲料蛋白的第四大来源,并有可能在世界食用蛋白质的供应方面发挥重大作用。
基于贝叶斯网络的城市轨道交通应急系统可靠性分析
Research on Reliability Analysis of Urban Rail Transit Emergency System Based on Bayesian Network
 [PDF]


Open Journal of Transportation Technologies (OJTT) , 2014, DOI: 10.12677/OJTT.2014.34015
Abstract:
通过分析城市轨道交通应急系统可靠性评价指标体系结构,建立了基于贝叶斯网络的城市轨道交通应急系统可靠性评价的贝叶斯模型。最后,以某地铁为例,建立基于贝叶斯的应急系统可靠性的模型,并利用贝叶斯推理及BayesiaLab工具进行了可靠性的计算。对计算结果进行了分析,并给出了提高该地铁应急系统可靠性的几点措施。
Urban rail transit emergency problem is very complicated, which is affected by many uncertain factors. In view of this, considering the advantages of Bayesian network in solving uncertainty problems, through the analysis of reliability of city track traffic emergency system evaluation index system, this paper has established the Bayesian model to evaluate the reliability of city rail transit emergency system. Finally, taking a subway as an example, the reliability of emergency system model is established based on Bayesian network and the calculation is carried out by using Bayesian’s reasoning and BayesiaLab tools. By analyzing the calculation results, some measures of improving reliability of the subway emergency system are given.
许昌市不同土地利用方式对土壤速效养分的影响
Characters on Available Nutrients of Soils under Different Land Use Types in Xuchang
 [PDF]


Open Journal of Soil and Water Conservation (OJSWC) , 2015, DOI: 10.12677/OJSWC.2015.34009
Abstract:
本文选取许昌市菜地、果园、耕地、苗圃、林地和城市绿地6种土地利用类型为研究对象,通过野外采样和室内分析对不同土地利用类型表层土壤(0~10 cm)的基本理化性质和速效养分特征进行了分析。结果表明:1) 土壤容重变幅为0.9~1.82 g/cm3;城市绿地 > 林地 > 苗圃 > 菜地 > 果园 > 耕地。2) 土壤pH值变幅为7.21~8.49,均值7.93,属碱性;菜地 > 果园 > 耕地 > 苗圃 > 林地 > 城市绿地。3) 土壤速效氮变幅12.55~47.48 mg/kg,均值为26.01 mg/kg,属于土壤养分分级6级水平;耕地 > 菜地 > 果园 > 林地 > 苗圃 > 城市绿地。4) 土壤速效磷含量变幅为11.54~78.86 mg/kg,均值26.71 mg/kg,属于土壤养分分级2级水平,呈现出富磷特征;城市绿地 > 果园 > 苗圃 > 菜地 > 林地 > 耕地。5) 土壤速效钾含量变幅在129.5~295.3 mg/kg之间,均值234.59 mg/kg,属于土壤养分分级1级水平;耕地 > 苗圃 > 菜地 > 果园 > 林地 > 城市绿地。
On the basis of field sampling and laboratory experiment, the basic physicochemical properties and the available nutrients of surface soils (0 - 10 cm) were analyzed between six land use types (vegetable field, orchard, cultivated land, nursery, forest land and urban green space) in Xuchang city. The e results showed that: 1) the soil bulk density was in the range of 0.9 - 1.82 g/cm3 and followed the order of urban green land > forest land > nursery > vegetable plots > orchard > culti-vated land. 2) The value of pH was in the range of 7.21 - 8.49, with the mean of 7.93, which inda-cated the alkaline feature. The order of pH was vegetable field > orchard > cultivated land > nur-sery > forest land > urban green space. 3) The content of soil available nitrogen ranged between 12.55 to 47.48 mg/kg, with the mean of 26.01 mg/kg and the level was the sixth. The order of available nitrogen was cultivated land > vegetable field > orchard > forestland > nursery > urban green space. 4) The content of soil available phosphorus was in the range of 11.54 - 11.54 mg/kg, with the mean of 26.71 mg/kg and the level was the second, indicating a highly rich in the phos-phorus. The soil available phosphorus followed the order: urban green space > orchard > nursery > vegetable field > forestland > cultivated land. 5) The soil available potassium content ranged be-tween 129.5 to 295.3 mg/kg, with the mean of 234.59 mg/kg and the level. The order of soil avail-able potassium was cultivated land > nursery > vegetable field > orchard > woodland > urban green space.
GGT综合地球物理资料人机交互解释系统的初步研究
高锐,贺知捷,潘喻,秦爽,,王学颖,褚德志,王华啸,李莉,余钦,楼海,吴至善
地球学报 , 1990,
Abstract: 为提高GGT解释性剖面的质量,构制地球物理解释模型,设计和研制了人机交互解释系统。整套系统即具有单一方法相对独立的快速求解任意复杂断面的人机交互功能,又可通过模型转换和参数的替换方式实现多方法的联合解释。系统设计以二维非均质任意形体的计算方法和物性参数间关系的研究为物理基础,引进鼠标器工作方式和调用DBASE图形软件包,增强了系统的图形编辑功能。系统的菜单式结构和用DBASEⅢ语写、串写的各子程序模块,不仅为采用新型数据库软件管理和查询物理场与模型数据开辟了较好的软件环境,而且使整个系统的软件结构灵活、清晰,便于改进、扩充。文章结尾以3个实例,介绍了人机交互系统在GGT地球物理解释中的应用。
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