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Search Results: 1 - 10 of 66470 matches for " 胡锦官 "
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石菖蒲及其有效成分对小鼠学习记忆的作用及α-细辛醚药物动力学研究
顾健,,谭睿
世界科学技术-中医药现代化 , 2003,
Abstract: 采用不同的动物实验方法,用石菖蒲去油煎剂(Ⅰ)、总挥发油(Ⅱ)、α-细辛醚(Ⅲ)进行实验。结果表明:对正常小鼠学习和记忆获得能力(跳台法试验),戊巴比妥钠造成的记忆获得障碍模型(电迷宫法试验),亚硝酸钠造成的记忆巩固不良模型(跳台法试验),乙醇造成的记忆再现缺失模型(跳台法试验)均有不同程度的促进作用和改善作用。测定其主要有效成分α-细辛醚在大鼠体内各项药物动力学参数,结果表明:α-细辛醚口服吸收快,在大鼠体内分布广泛。以上实验结果还表明:石菖蒲对学习记忆作用的主要有效部位是总挥发油(Ⅱ),其中α-细辛醚(Ⅲ)是其主要有效成分,去油煎剂(Ⅰ)有一定作用,但作用较弱。
四川南充地区棕背伯劳的繁殖习性
天培,,罗贵平,李奎,宋跃,
动物学杂志 , 2006,
Abstract:
Clinical Characteristics of Nephrotoxicity of Caulis AristolochiaeManshuriensis and Related Experimental Research
木通肾毒性的临床特点及实验研究概况

Hu Jinguan,Long Shaojiang,Gu Jian,
,龙绍疆,顾健

世界科学技术-中医药现代化 , 2003,
Abstract: The nephrotoxicity of Caulis Aristolochiae Manshuriensis has got more and more attention in China and abroad. This article makes the textual research of Caulis Aristolochiae Manshuriensis from the viewpoint of herbalism and summarizes the clinical characteristics of its nephrotoxicity as well as its experimental research carried out in animal models, toxicity mechanisms and pharmacokinetics in recent years.
华南瓜类疫霉的RAPD遗传多样性分析
吴永,蒋雅琴,黄思良,车江旅,付岗,,黎炎
菌物学报 , 2015, DOI: DOI:10.13346/j.mycosystema.140059
Abstract: 为了解来自广东和广西的瓜类疫霉的遗传多样性,利用从180条RAPD引物中所筛选出的多态扩增性强、重复性好的12条引物,对分离自两省区的96株瓜类疫霉进行了全基因组DNA遗传多样性分析和指纹图谱构建。通过对供试菌株的RAPD-PCR扩增,共获得135条DNA标记谱带,其中124条为多态性谱带,多态检测率为91.9%。利用NTSYSpcVersion2.1软件对供试菌株间的遗传距离进行聚类分析并构建系统树,以遗传相似系数0.81为阈值,将96个供试菌株划分为12个RAPD群,多数分离物之间遗传相似性较低,在DNA水平上存在显著的遗传变异,具有较丰富的遗传多样性。不同地区间菌株的遗传分化程度不同,分离自黄瓜的菌株遗传分化明显高于分离自冬瓜的菌株。RAPD群与菌株地理来源、分离寄主、致病力、交配型及甲霜灵抗性均无明显的相关性。
华南瓜类疫霉种群的致病力及其寄主嗜好性
吴永,,黄思良,陆少峰,车江旅,付岗
植物保护学报 , 2012,
Abstract: 2007―2010年从广西、广东两省(区)9个样区采集冬瓜、黄瓜疫病显症植株,经分离纯化鉴定共获得193株瓜类疫霉。采用菌丝块无伤接种法,将菌株接种冬瓜和黄瓜,测定、比较其对供试寄主植物的致病力及其寄主嗜好性。结果表明,病原群体对寄主植物冬瓜和黄瓜的致病力均存在明显分化现象,可分为强致病力、中等致病力及弱致病力3大类群;来自不同地区的瓜类疫霉对寄主植物冬瓜和黄瓜的致病力明显不同,多数样区以强致病力菌株占优势。瓜类疫霉种群中存在3种寄主嗜好型菌株,A型菌株对冬瓜和黄瓜均表现强致病力,与原始分离寄主无关;B型菌株仅对原始分离寄主冬瓜表现强致病力,而对黄瓜则表现为弱或中等致病力;C型菌株仅对原始分离寄主黄瓜表现强致病力,而对冬瓜则表现为弱或中等致病力。
全人源化抗结肠癌单链抗体基因的克隆和表达
朱建高,, 李跃辉, , 周国华, 志伟, 孙去病, 李小玲*
生物工程学报 , 2001,
Abstract: 分离大肠癌患者外周血单个核细胞(pbmc),在体外用灭活的大肠癌细胞再次致敏后,经ebv转化,然后用有限稀释法克隆分泌抗大肠癌细胞抗体的b细胞;多次pcr扩增和克隆其抗体可变区基因(vh/vlcdna),并用编码(gly4ser)3的互补序列连接成scfvcdna(vhlinkervl),经酶切克隆入载体fuse5rf,使之呈现于噬菌体表面。通过用3轮肿瘤细胞和正常人pbmc淘选后,elisa检测80%的单克隆噬菌体抗体显示了很强的阳性反应。以上结果初步说明:联合应用体外致敏、ebv转化、pcr扩增和噬菌体呈现技术制备高亲和力全人源化的抗肿瘤抗体是可行的.
云南省西北部水文站网分析与优化调整
Hydrologic Network Analysis and Optimal Adjustment in the Northwest Yunnan Province
 [PDF]

张坤, 陈岗, 张伦, , 蔡文静
Journal of Water Resources Research (JWRR) , 2016, DOI: 10.12677/JWRR.2016.53035
Abstract:
通过对云南省西北部大理、迪庆、怒江州三个州境内的流域、河流、水利水电工程和水文站网进行调查,对各类监测站网密度及布局进行分析,结果表明云南省西北部除雨量站网、蒸发站网和地下水站网外的其它各类监测站网密度基本达到世界气象组织及《水文站网规划技术导则》规定的密度要求。根据完全水文空白河流与流量测验空白河流评价方法,对河流的水文监测覆盖程度进行分析与评价,结果表明云南省西北部部分流域河流存在流量测验空白河流。根据成果提出站网优化调整对策。
Through investigating the river basin, rivers, hydropower engineering and hydrologic network in the northwest of Yunnan province, which consists of Dali, Diqing and Nujiang states, the networks’ density and layout of all kinds of monitoring stations is analyzed. The results show that the density of monitoring station network has reached basic requirements of World Meteorological Organization and “Technical Regulations for Hydrologic Network Design” except for the precipitation, evaporation and groundwater station networks. Based on the evaluation methods of the hydrologic completely blank river and the flow measurement blank river, the degree of rivers’ coverage is analyzed and evaluated. It is shown that the flow measurement blank rivers exist in some river basin in the northwest of Yunnan province. Finally, some optimized and adjusted countermeasures are put forward.
Cloning and Expression of Human Anti-colonic Cancer Single-chain Fv Fragment
全人源化抗结肠癌单链抗体基因的克隆和表达

J G Zhu,G C Li,Y H Li,J Y Hu,G H Zhou,Z W Hu,Q B Sun,X L Li,
朱建高
,,李跃辉,,周国华,志伟,孙去病,李小玲

生物工程学报 , 2001,
Abstract: We report a new strategy for the generation of human anticolon cancer monoclonal antibodies based on the molecular cloning and expression of immunoglobulin variable region cDNAs derived from peripheral blood mononuclear cells (PBMC) that were transformed by EBV. The immortalized B cells secreting tumor-specific antibodies were identified by HRT-18 cell ELISA and cloned by limiting dilution. Heavy- and light-chain VH-CH1 (gamma) and V kappa-C kappa cDNAs were rescued from ELISA-positive cells wells by RT-PCR. VH and V kappa were amplified by 2nd PCR and linked them together by 3rd PCR assembly with the use of a (Gly4Ser)3 linker. The ScFv cDNAs were then cloned into the fUSE 5 vector and displayed on phage. Phage clones were selected on HRT-18 cells and normal human PBMC. ELISA tested phage clones randomly picked after each panning step. > 80% of the clones showed a strong ELISA reaction, demonstrating the effectiveness of the panning procedure for selecting anticolon cancer antibodies. This approach offers an effective method by combining in vitro immunization, B-cell expansion for enrichment of specific B-lymphocytes, PCR gene cloning and phage display to make high-affinity human anticolon cancer monoclonal antibody molecules.
实体bean容器管理持久化研究与实现
建华 荷卿 陈宁江?
计算机科学 , 2005,
Abstract: 实体bean是一种重要的j2ee应用组件,具有持久保存状态的特性。实体bean容器是实体bean的运行环境,持久化管理器是其中具体负责持久化的组件。本文研究了以关系数据库作为存储介质实现容器管理持久化的理论,提出了一种灵活高效的实现方法。该持久化管理器通过静态映射模型创建持久化方案,并创建动态模型以响应客户请求。在实体bean容器并发访问控制机制的帮助下,该持久化管理器能够保证实体bean高效可靠的运行。该持久化管理器和容器已经在中科院软件所自主研制的j2ee应用服务器onceas中得到实现。
论西方违宪审查制度的发展趋势

比较法研究 , 1987,
Abstract:
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