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Search Results: 1 - 10 of 312212 matches for " 王健 "
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Solubilization and Refolding of Inclusion Body Proteins Expressed in Bacterial Host

Bioprocess (BP) , 2012, DOI: 10.12677/BP.2012.24023
Abstract: 原核细胞表达外源基因常常会导致包涵体的产生。包涵体的复性是制备有效的功能蛋白质的必需途径。每一种蛋白质的基本结构与性质不同,决定了包涵体中外源蛋白质的复性方法多种多样。本文总结并介绍了目前在包涵体复性方面的几种方法与策略。初步讨论了在复性过程中蛋白质的结构变化。
Inclusion bodies produced in Escherichia coli are composed of densely packed denatured protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive forms is cumbersome, results in poor re- covery and accounts for the major cost in production of recombinant proteins from E. coli. With new information available on the structure and function of protein aggregates in bacterial inclusion bodies, it has been possible to de- velop improved solubilization and refolding procedures for higher recovery of bioactive protein indexed at this paper.
A Finite Spectral Finite Element Method for Incompressible Navier-Stokes Equations

International Journal of Mechanics Research (IJM) , 2014, DOI: 10.12677/IJM.2014.33004
A finite element method combined with the idea of finite spectral method was represented to solve the partial differential equations. In each element, finite spectral interpolation functions were used. The finite spectral finite element method was used to solve incompressible Navier- Stokes equations in this paper. The results of 2D-square cavity flow are presented and are in agreement with the accepted benchmark solutions.
Discussion on the Position of Fixed End of the Tall Building Structure

, 敦强
Journal of Security and Safety Technology (JSST) , 2013, DOI: 10.12677/JSST.2013.12002
In the practical engineering design, structural engineering staffs did not attach importance to fixed end of high-rise building structure. This will cause material waste, and will also leave hidden troubles to the high-rise building structure. Some of the structural designers have mistakes in the definition of fixed end of the tall building, who regard them as mechanics embedment, but in fact it should be strength-mounted. Specification makes requirements clearly about the roof of the underground layer as embedding end. In this paper, three basement flat area types of high-rise building structure embedded end have been studied. It comes to conclusions 1) there are different embedding positions in different basement flat forms under normal circumstances, and 2) the lateral stiffness ratio of ground floor with the relevant range of underground floor is the key to measure whether the basement roof can be used as a high-rise building structure embedded fixed end.

中外法学 , 1998,

中外法学 , 2001,

中外法学 , 2005,

比较法研究 , 1997,

比较法研究 , 1996,

比较法研究 , 2003,
Abstract: 不为商海淘金所动,却愿史海钩沉,是乃传统中国文人士子之常道。然今日为之,实属难能可贵。近代中国社会变革,与德国多有相关。其中德国法制对中国法制的影响,尤为学界之人每每称道。然德国法究竟如何在中国展开影响?其途径与方式究竟有哪些?细细考察,可知这方面的研究并不多,王健教授对于“青岛特别高等专门学堂”的用心与考证,当为可以成史之作。至少,我们又可从中了解到一段鲜为人知的史实。

比较法研究 , 1998,
Abstract: 闪现在我脑海中的念头,是心灵与经验相碰撞而生出的小火花。它们不召而来,又婉拒邀请。法律是理想与现实的契合点,就仿佛莲花,它的根深深地植入泥土,而花苞和花瓣向天空伸展。法律是一种把物质利益的摩擦转化成理想物之光的艺术。
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