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In this study, the protoplasts from tobacco BY-2 (Nicotiana tabacum) were used to determine whether cells can be induced to expand in a preferential direction in response to an externally applied centrifugal force. Spherical protoplasts were embedded into agarose block. Constant uniaxial pressure released by a centrifugal force was imposed on the block. The results showed that protoplasts elongated with a preferential axis oriented perpendicularly to the applied force direction, according to a certain dose-dependent relation between the intensity of centrifugal force and the stress induced responses. The microtubules with centrifuged protoplasts were found to be oriented parallel to the centrifugal force direction.