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Search Results: 1 - 10 of 91011 matches for " 柴志欣 "
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牦牛MC1R基因的多态性研究
陈思,,王永,钟金城
生物技术通报 , 2014,
Abstract: 旨在为探究牦牛MC1R基因多态性与毛色形成的相关性,利用PCR-SSCP和DNA测序技术,对64头牦牛(33头黑色九龙牦牛,31头白色天祝白牦牛)的MC1R基因多态性进行检测。结果表明:天祝白牦牛和九龙牦牛均有3种基因型(AA、BB、AB),但天祝白牦牛的多态性较低,而九龙牦牛表现为中度多态。经χ2适合性检验,2个牦牛品种在该基因多态位点上均偏离Hardy-Weinberg平衡。测序结果表明BB型与AA型在该片段的第179位碱基处存在C→A单碱基突变;第214位碱基处发生T→C突变。
?过渡季节vav空调系统送风温度的优化控制策略
?晋,小峰,
天津大学学报(自然科学与工程技术版) , 2009,
Abstract: ?变风量空调系统中,如何在良好室内空气品质与节能之间进行权衡一直是研究的难点.通过对多区域变风量空调及其控制系统进行分析研究,按照ashrae通风标准对新风量的要求,针对混和送风系统提出并在trnsys仿真平台上分析了送风温度优化控制方案在不同负荷条件下的控制效果.研究结果表明,室外新风温度低于室内温度设定值时,送风温度的设定值受到设计风量和运行时刻各个空调区域风阀开度大小的限制.当过渡季节早晚的新风温度低于室内设定温度的工况时,若将送风温度由13,℃升高到16,℃,不但可以减少2.8%的空调系统能耗,而且有助于改善空气品质.
牦牛POMC基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of POMC Gene of GBJD Yak

陈攀攀,,钟金城
- , 2015, DOI: 10.7606/j.issn.1004-1389.2015.07.002
Abstract: 为探讨工布江达牦牛POMC基因的分子结构特征,通过分子克隆技术获得工布江达牦牛POMC基因全序列,采用生物信息学方法对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构等进行预测与分析。结果表明,工布江达牦牛POMC基因包含1个798 bp的开放阅读框,编码265个氨基酸,其编码蛋白属于亲水性蛋白,具有明显的信号肽,二级结构主要以无规卷曲和α-螺旋为主。以POMC基因构建的邻接系统发育树表明,工布江达牦牛POMC与野牦牛、普通黄牛、水牛和羊等品种的POMC遗传距离较近,具有高度同源性。
In order to explore the structures of POMC gene of GBJD yak, the complete sequence of GBJD yak POMC gene was cloned by molecular cloning technique, Some characters of POMC gene and its encoded protein sequences were predicted and analyzed by methods in bioinformatics, such as the general physical and chemical properties, hydrophobicity, signal peptide and secondary structure.The results showed that the full-length of GBJD yak POMC gene contained a complete ORF(798 bp), which could encode 265 amino acid, the protein encoded by yak POMC gene had obvious signal peptide, it was a hydrophilic protein, the secondary structure was mainly composed of Helix and Coli. The POMC gene of GBJD yak,Bos mutus, Bos taurus,Bubalus bubalis and Ovis were close in phylogenetic tree,which had high homology.
麦洼牦牛H-FABP和MC4R基因的克隆及生物信息学分析
Cloning and Bioinformatical Analysis of H-FABP and MC4R Gene in Maiwa Yak

,钟金城,,,文勇立
- , 2015, DOI: 10.7606/j.issn.1004-1389.2015.05.002
Abstract: 为探究H-FABP、MC4R基因在麦洼牦牛中的结构和功能,克隆测序麦洼牦牛H-FABP、MC4R基因的编码区全序列,并利用生物信息学软件分析其编码区序列、蛋白质结构、功能及进化关系。结果表明,麦洼牦牛的H-FABP 基因全长440 bp,其中编码区为401 bp,编码133个氨基酸;MC4R基因全长1 434 bp,其中编码区999 bp,编码332个氨基酸。麦洼牦牛H-FABP和MC4R基因核苷酸序列与普通牛、绵羊、猪、人和小鼠的核苷酸比较,其一致性分别为83%~99%、85%~99%,其中与普通牛的最高(99%、99%),其次是绵羊(96%、95%),与小鼠的最低;说明在不同物种中,这2个基因属于直系同源的基因。H-FABP和MC4R蛋白均为疏水性结构蛋白。
To study structure and function of H-FABP and MC4R gene in Maiwa yak, so as to provide a theoretical basis for breeding and utilization in the future. The CDS sequence of the H-FABP and MC4R gene was cloned by RT-PCR method, coding region sequence.Protein structure and phylogenetic relationship were analyzed by bioinformatics software. The results showed that the cDNA length of H-FABP gene was 440 bp and its CDS sequence was 401 bp encoding a putative protein of 133 amino acid (AA) residues.The cDNA length of MC4R gene was 1 434 bp and its CDS sequence was 999 bp encoding a putative protein of 332 amino acid (AA) residues. The nucleotide sequence identity of the H-FABP and MC4R gene between cow, sheep, pig, person and mouse were 83%-99%, 85%-99% respectively. The homology of ordinary cattle was highest with yak(99%, 99%), followed by sheep(96%, 95%)and mouse, the mouse is the lowest. The results also indicated that they were orthologous genes in different species. H-FABP and MC4R were structural proteins with hydrophobic.
牦牛和犏牛 DDX25/ GRTH基因序列及其在睾丸组织的表达水平
Sequencing and Expression of DDX25/GRTH mRNA in Yak and Cattle-yak Testis

张思源,,彭娅林,钟金城
- , 2015, DOI: 10.7606/j.issn.1004-1389.2015.10.001
Abstract: 为了解 DDX25基因的结构、功能及对犏牛雄性不育的影响。采用RT-PCR技术克隆获得牦牛和犏牛的 DDX25基因,对核苷酸序列和氨基酸序列进行生物信息学分析,运用实时荧光定量PCR技术检测睾丸组织 DDX25 mRNA的表达情况。结果表明,牦牛和犏牛 DDX25基因编码区序列全长均为1 452 bp,编码483个氨基酸,氨基酸存在19个磷酸化位点,无信号肽。牦牛睾丸组织 DDX25基因表达水平显著高于犏牛, DDX25基因在犏牛睾丸组织的低表达与其雄性不育存在一定关系,该基因可作为研究犏牛雄性不育的候选基因。
To understand the structure of DDX25, gene function and its impact on cattle-yak male sterility, DDX25 gene was cloned by RT-PCR from cattle-yak and yak, conducting bioinformatics analysis based on the sequences of nucleotide and amino acid, and DDX25 mRNA expression in testis was detected by Real-time PCR.The results showed that DDX25 gene coding sequences in yak and cattle-yak is the full length of 1 452 bp, encoding 483 amino acids with 19 phosphorylation sites, without signal peptide. DDX25 gene expression level in testis of yak were significantly higher than in testis of cattle-yak.Low expression of DDX25 gene in testis of cattle-yak has a certain relationship with their male sterility, so the gene can be used as a candidate gene for male sterility cattle-yak.
牦牛 DKK1基因多态性及其与生长性状的相关性
Association of Single Nucleotide Polymorphism of DKK1 Gene with Growth Traits in Yak

,,,钟金城,陈智华
- , 2017, DOI: 10.7606/j.issn.1004-1389.2017.07.003
Abstract: 选取麦洼牦牛、类乌齐牦牛、申扎牦牛、帕里牦牛和斯布牦牛5个群体共287头个体,采用直接测序法检测牦牛 DKK1基因的SNP位点,分析其与生长性状的相关性。结果表明,牦牛 DKK1基因存在2个突变位点, g.983A→G位点和g.1075C→G位点;位点g.983A→G在斯布牦牛中处于Hardy-Weinberg不平衡状态,其余位点处于平衡状态。在遗传多态性研究中,2个SNP位点均为中度多态。2个位点不同基因型与体高、体斜长、胸围、管围和体质量的相关分析结果显示,位点g.983A→G与体高、体斜长、胸围和体质量均具有相关性;位点g.1075C→G与生长性状不相关。
To investigate the genetic polymorphism of Dickkopf-1 ( DKK1) gene in yak and reveal the association between SNPs detected and growth traits,and looking for alternative auxiliary marker. This study selected 287 individuals which include five yak breeds named Maiwa,Leiwuqi ,Shenzha ,Pali and Sibu,to detect the SNPs of the DKK1 gene in yaks and its correlation with the growth traits of yak. The results showed that:there are two mutations in the DKK1 gene,each of them has three different genotypes. The χ value of g.983A→G site in SB yak was 6.324 2,which meant the site was out-off-balance of the Hardy-Weinberg equilibrium (HWE),but the rest of sites matched the balance of the HWE;the distribution of both SNPs were moderate polymorphic;the correlation analysis between different genotypes of two SNPs of DKK1 gene and body height,body length,chest bust,cannon circumference,body mass and other growth traits showed that g.983A→G site was associated with body height,body length,chest bust,and body mass,but no significant difference was detected between g.1075C→G and growth traits.
野牦牛线粒体基因组序列测定及其系统进化
钟金城?,,杰?,王永?,杨万远?,拉环?
生态学报 , 2015,
Abstract: 野牦牛属高寒地区的特有物种,是我国最珍贵的野生动物遗传资源之一,已被列为国家一级重点保护动物。对野牦牛mtdna进行全序列测定和结构分析,并基于线粒体基因组序列对其系统发生进行了探讨。结果表明:(1)野牦牛线粒体基因组全序列的大小为16322bp,整个基因组由37个编码基因和d-loop区组成;22个trna基因序列长度为1524bp、2个rna基因序列长度为2528bp、13个编码蛋白基因序列长度为11420bp、d-loop区长度为892bp。基因组中无间隔序列,基因间排列紧密,基因内无内含子。(2)野牦牛具有较丰富的遗传多样性。(3)分子系统发生关系显示牦牛为牛亚科中的一个独立属,即牦牛属(poephagus),牦牛属包括家牦牛(poephagusgrunniens)和野牦牛(poephagusmutus)2个种。野牦牛线粒体基因组全序列的获得和结构解析对研究牦牛的起源、演化和分类,以及野牦牛遗传资源的保护、开发和利用均具有重要的理论和实际意义。
CAPN1和CAST基因在牦牛不同组织中的表达差异研究
姚慧,陈智华,钟金城,曾贤彬,
河南农业科学 , 2014,
Abstract: 为检测钙蛋白酶基因(CAPN1)和钙蛋白酶抑制基因(CAST)在牦牛心、肝、脾、肺、肾、胃、眼肌、腹肌等不同组织中的表达水平,采用实时荧光定量PCR技术分析这2个基因的表达规律。结果显示,CAPN1和CAST基因在牦牛的8种组织中均有表达,但在不同组织的表达量存在差异。CAPN1基因在眼肌中的表达量最高,其次为腹肌,在肌肉中的表达量显著高于内脏组织,内脏组织中,在肝脏的表达量最高,其次为肾脏,胃中的表达量最低;CAST基因在胃中的表达量最高,其次为眼肌、腹肌,在肾脏中的表达量最低。
西藏牦牛SRAP遗传多样性及分类进化研究
赵上娟,钟金城,,张成福,信金伟,陈智华
生物技术通报 , 2012,
Abstract: 用4对SRAP分子标记引物对西藏11个牦牛类群和四川麦洼牦牛的DNA进行扩增,研究其遗传多样性和分类关系。结果表明,在335头牦牛中,共得到29个基因位点,其中有19个多态位点,多态率占65.52%。12个牦牛群体间的Nei's遗传多样性和Shannon多样性指数分别为0.0482和0.0734,遗传相似系数在0.7811-0.9891。巴青牦牛和康布牦牛的遗传多样性指数较其他类群高,分别为0.0955和0.0900;桑日牦牛类群的遗传多样性指数最低,仅为0.0085。这些结果表明,12个牦牛类群的SRAP遗传多样性较低。根据Nei's遗传距离,利用UPGMAM构建聚类关系图结果显示,嘉黎牦牛、帕里牦牛、类乌齐牦牛、桑桑牦牛、康布牦牛、巴青牦牛、丁青牦牛、斯布牦牛和麦洼牦牛聚为一大类,然后依次才与桑日牦牛、工布江达牦牛和江达牦牛相聚在一起,显示在SRAP分子遗传标记所反映的牦牛基因组的遗传结构中,江达牦牛、工布江达牦牛和桑日牦牛与其他牦牛群体间的亲缘关系较远,牦牛的这种亲缘关系与其地理分布也不一致,说明这12个牦牛的起源、演化关系较复杂,有待于进一步研究分析。
西藏牦牛的rapd遗传多样性及其分类研究
,赵上娟,姬秋梅,张成福,信金伟,钟金城
牲畜兽医学报 , 2011,
Abstract: ?为了解西藏地区牦牛品种或类群的遗传多样性和亲缘关系,本研究从33个rapd多态性引物中筛选出8个条带清晰且多态性丰富的引物对西藏地区的巴青牦牛、类乌齐牦牛、丁青牦牛、桑日牦牛、工布江达牦牛、江达牦牛、康布牦牛、桑桑牦牛、嘉黎牦牛、帕里牦牛、斯布牦牛等11个类群的核基因组dna进行了rapd分析,并用nei氏标准距离和upgma聚类法分析了类群间的亲缘关系。结果表明:(1)西藏牦牛类群的遗传多样性指数变异范围在0.1857~0.4053之间,其中帕里牦牛最小(0.1857),说明相对较纯,群体较整齐;而工布江达牦牛最大(0.4053),显示该群体内部具有较多的遗传变异。(2)在11个类群中,其遗传多样性指数大小分别为:工布江达牦牛(0.4053)>江达牦牛(0.3536)>斯布牦牛(0.3448)>康布牦牛(0.3428)>嘉黎牦牛(0.3323)>桑日牦牛(0.2823)>巴青牦牛(0.2793)>桑桑牦牛(0.2698)>丁青牦牛(0.2597)>类乌齐牦牛(0.2241)>帕里牦牛(0.1857),具有西藏东部牦牛类群遗传多样性相对较高,而西部牦牛类群遗传多样性相对较低的趋势,预示着西藏东部可能是牦牛的起源地之一。(3)遗传距离构建的分子聚类关系图表明:西藏11个牦牛类群可分为2大类,帕里牦牛(pl)为一类,其余10个牦牛类群为另一类。综上所述,西藏牦牛具有较丰富的遗传多样性,品种或种群内的遗传分化显著,这是西藏牦牛业持续发展和牦牛适应外界环境的遗传基础,是将来培养牦牛新品种或品系的重要基因资源;西藏牦牛品种可分为2大类群。
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