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Search Results: 1 - 10 of 176102 matches for " 杨辉娜 "
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骨活素基因转染的兔骨髓基质干细胞复合多孔丝素蛋白支架体外构建组织工程骨
Construction of Tissue Engineering Bone with Osteoactivin Gene Transfected Rabbit Bone Marrow Stromal Cells and Porous Silk Fibroin Scaffold in Vitro
 [PDF]

王涵, 征毅, 程峰, 潘广嗣, 易晓, 孙晋, 曹依, 袁林
Advances in Clinical Medicine (ACM) , 2014, DOI: 10.12677/ACM.2014.42006
Abstract:

目的:用腺病毒表达载体将骨活素基因转染到兔骨髓基质干细(BMSCs),复合多孔丝素蛋白支架体外构建组织工程骨。方法:用表达骨活素基因的腺病毒载体转染体外培养的兔BMSC,免疫组化、原位杂交染色和蛋白印迹方法检测细胞骨活素的表达,并通过流式细胞仪和ALP活性检测分析其对细胞增殖、分化的影响。然后将转染后细胞接种到多孔丝素蛋白支架上,扫描电镜观察细胞贴附、生长状况。结果:转染后,骨活素基因在mRNA水平和蛋白水平均有表达;S期细胞比例和ALP活性明显增高。扫描电镜见转染细胞分布均匀,伸展良好。结论骨活素基因可高效转染兔BMSC,且促进细胞增殖及成骨转化。转染后细胞在多孔丝素蛋白支架上生长良好,骨活素基因治疗的组织工程骨构建成功。
Objective: Rabbit bone marrow stromal cells (BMSCs) infected by a recombinant adenoviral vector carrying the osteoactivin gene (Ad-OA) were seeded into porous silk fibroin scaffold to construct tissue engineering bone in vitro. Method: Ad-OA infected RBM SC cultured in vitro and the expression of OA in these cells after infection were determined by in situ hybridization and immune ohistochemical analysis. OA productions were confirmed by western blot analysis of the supernatant collected from the cells. The changes of cellular proliferation and differentiation in the cells were observed by flowcytometry and ALP activity analysis. OA transduced cells were then seeded into porous silk fibroin scaffolds. The attachment and growth of the cells on the scaffold were examined using SEM. Results: The expression of OA was confirmed in mRNA and protein levels in the cells after infection and the presence of OA was detected in the supernatant of the cells. In addition, cellular proportion in S period and ALP activity obviously increased in the cells. SEM examination revealed extensive cellular attachment and growth on the porous silk fibroin scaffolds composite in 1 day. Conclusion: Ad-OA could infect RBMSC with high efficiency and promote cellular proliferation and osteoblast conversion. The cells after infection grew well on a porous silk fibroin scaffold. Tissue engineering bone used to regional gene therapy is constructed successfully.

Overview on AES-Rijndael Algorithm
AES—Rijndael算法综述

,王丽,于戈
计算机科学 , 2002,
Abstract: 今年夏天,美国NIST将颁布新的数据加密标准,比利时的Joan Daemen和Vincent Rijmen提交的Rijndael算法作为AES(Advanced Encryption Standard)将被美国政府和团体广泛用作为2001年以后的加密敏感性信息的标准。其实,早在1997年NIST就公开征求AES作为2001年以后的数据加密标准,同时提出了对AES的几点要求,此项举措得到世界密码界的积极响应。AES征集通告发出之后,许多国家、企业和个人提出了自己的方案。1998年8月,AES召开第一次候选会,确定15个算法入围;1999年3月,AES召开第二次候选会,有5个算法入围;2000年10月,NIST选出Rijn-dael作为AES,可以说Rijndael算法代表国际上分组加密算法的最高水平。2001年2月28日颁布FIPS-AES草案,2月29日起,NIST开始为期90天的公众评论,正式的AES将在今年夏天颁布。
壳聚糖絮凝法回收鱼糜漂洗水中水溶性蛋白质的工艺研究
许永安,吴靖,苏捷,
南方水产科学 , 2011, DOI: 10.3969/j.issn.2095.0780.2011.03.001
Abstract: 采用壳聚糖作为絮凝剂进行鱼糜漂洗水中水溶性蛋白质的回收试验。分别通过体系的ph、回收温度和壳聚糖絮凝剂加入量的单因素试验及这3个因素的正交试验,研究其对蛋白质回收率、透光率和化学耗氧量(cod)去除率的影响,得出壳聚糖絮凝法回收40ml已知蛋白质质量浓度(9.0mg·ml-1)的鱼糜漂洗水中水溶性蛋白质的最佳工艺条件为体系ph8.0,回收温度35℃,1%壳聚糖添加量为1.5ml。该工艺获得的蛋白质回收率为73.17%,透光率87.50%,cod去除率47.20%。
一种基于改进蚁群算法与GIS的多约束配送中心选址方法
赵仁,,邵静
地球信息科学学报 , 2015, DOI: 10.3724/SP.J.1047.2015.00172
Abstract: 针对单一指派约束和容量约束的设施选址问题(SingleSourceCapacitatedFacilityLocationProblem,SSCFLP),建立了一种基于改进蚁群算法与GIS的配送中心选址方法。构建了以总成本费用最小为目标的配送中心选址模型;提出了适合求解SSCFLP问题的改进双层蚁群算法,将求解过程划分为彼此关联的设施选择层和需求指派层2层蚁群,采用改进的全局信息素更新策略加强双层蚁群交流,并对迭代最优解的指派关系进行局部优化;将方法应用于汽车配送中心的选址,利用GIS工具构建选址空间。实验结果表明,该选址方法能找到质量较好的选址及指派结果,对于求解同类问题具有较强的借鉴意义。
aes—rijndael算法综述
王丽 于戈?
计算机科学 , 2002,
Abstract:
面向人工肌肉驱动器的无模型自适应滑模控制研究
,郝丽,孙智涌,陈洋
- , 2017, DOI: 10.12068/j.issn.1005-3026.2017.11.020
Abstract: 摘要 针对人工肌肉驱动器模型的控制策略较为复杂、控制参数较多、调节过程较为繁琐、通用性较差等问题.首先基于梯度法设计了无模型自适应滑模控制器(MFASMC)并对其稳定性进行了证明.然后,利用该控制器分别对离子交换聚合金属(IPMC)以及气动人工肌肉(PAM)两种典型人工肌肉驱动器进行控制仿真实验,从而对其控制精度、适应性及通用性进行验证.由仿真结果可知,通过设计控制器参数的调节规律,MFASMC算法可以实现对控制参数的快速在线调节,故对于不同种类的人工肌肉驱动器避免了繁杂的数学建模过程及控制参数调节过程,具有良好的适应性及通用性.
Abstract:The traditional control strategies for artificial muscle actuators based on the control object model have drawbacks such as complex in control laws, too many control parameters which is tedious to adjust, and having poor universality. Thus, a model free adaptive sliding mode controller (MFASMC) was designed based on the gradient method and proved in stability. Then, the new algorithm was simulated to contorl the identification models of IPMC(ionic polymer metal composite) and PAM(pneumatic artifical muscle) respectively. The results proved that the control parameters can be adjusted online by the new MFASMC algorithm rapidly, by designing the control parameters regulation rules. In this way, MFASMC makes the modeling process is not necessary. Moreover, the good control effects can be obtained for various artificial muscle actuators with no need to adjust any control parameters. Therefore, MFASMC has good adaptability and universality.
针对气动肌肉仿生肘关节抖振现象的Kalman-PID控制
Kalman-PID control for chattering phenomena of bionic elbow joint actuated by pneumatic artificial muscles

,,郝丽,陈洋,薛帮灿
控制理论与应用 , 2017, DOI: 10.7641/CTA.2017.60535
Abstract: 本文为了抑制气动人工肌肉(PAM)抖振现象, 首先利用PID控制律近似代替其数学模型, 求得其离散状态方程并代入到离散卡尔曼递推公式中, 进而提出基于PID控制参数的卡尔曼PID(Kalman-PID, KPID)控制算法. 为了 验证算法的有效性, 以3自由度PAM仿生肘关节为控制对象, 分别利用PID及KPID控制器对其进行位姿控制. 由实验结果可知, 该算法相较PID控制器拥有更高的控制精度, 提升了系统的鲁棒性, 能够有效抑制由系统过程误差及测量误差所引起的PAM抖振现象, 从而使仿生肘关节运动更加平稳. 此外, 不同于传统卡尔曼滤波算法与控制算法相结合的方式, KPID控制算法无需事先知道被控对象精准的数学模型及噪音特性的先验知识, 从而避免复杂的数学建模过程, 扩大了卡尔曼滤波算法的应用范围.
For restraining the chattering phenomena of pneumatic artificial muscles (PAMs) caused by process errors and measurement errors, the mathematical model of a PAM was replaced by PID control law whose discrete state equations was solved and taken into the discrete Kalman recursive formulas. Then, the Kalman-PID (KPID) controller based on the PID control parameters was proposed. In order to verify effectiveness of KPID controller, a 3-DOF bionic elbow joint actuated by three pneumatic artificial muscles was treated as the controlled object and controlled via PID controller and KPID controller, respectively. From the results, KPID controller has higher control precision and better robustness than PID controller. KPID controller can restrain the chattering phenomena effectively, which makes the bionic elbow joint rotate smoothly. Moreover, KPID controller does not need the accurate mathematical model of controlled objects and priori knowledge of the noise characteristics, which avoids complicated modeling process and expands the application range of Kalman filtering algorithm.
发作间期/发作期SPECTrCBF显像在癫痫灶定位诊断中的价值
,罗焱,胡云,苏红媛,,张青
第三军医大学学报 , 2012,
Abstract: 目的探讨发作间期/发作期SPECT局部脑血流灌注显像(regionalcerebralbloodflow,rCBF)在癫痫术前定位诊断中的价值。方法回顾性分析第三军医大学新桥医院神经外科行外科手术治疗的115例癫痫患者,术前分别行发作间期/发作期SPECT显像、常规脑电图(electroencephalogram,EEG)、视频脑电图(vedio-EEG,VEEG)、CT或MRI检查,术中行皮层脑电图(electrocorticogram,ECoG)监测,与发作间期/发作期SPECT、EEG、VEEG、CT和MRI检查结果进行比较分析。结果发作间期/发作期SPECT检出癫痫的阳性率为90.4%,高于EEG的37.4%(P<0.05)、VEEG的82.6%(P>0.05)、CT的48.8%(P<0.05)和MRI的54.2%(P<0.05)。与术中ECoG结果比较,发作间期/发作期SPECT对癫痫灶定侧率为91.3%,定位率为71.2%。结论术前发作间期/发作期SPECT显像与ECoG在癫痫灶定位诊断上具有较高的一致性,在术前综合定位诊断中具有重要意义。
福建省农业科学院主办期刊现状及改革建议
刘新永,小萍,林玲,王景
中国科技期刊研究 , 2012,
Abstract: 根据科技期刊发展趋势,结合分析福建省农业科学院主办期刊现状,提出进一步促进福建省农科院主办期刊发展的建议及对策。
近500年南极涛动指数重建及其变率分析
张自银,龚道溢,何学兆,,冯胜
地理学报 , 2010, DOI: 10.11821/xb201003001
Abstract: 南极涛动是南半球大气环流的主要模态,在多种尺度上对南半球及北半球部分地区的气候系统产生重要影响。在对树轮、珊瑚、冰芯等多种代用资料进行挑选与主成分分析的基础上,重建了公元1500年以来南半球夏季(当年12月-次年2月)的南极涛动指数。重建序列的解释方差(r2)、误差减少量(RE)平均值分别为59.9%、0.47,较高的r2、RE表明重建具有了较高的可信度;而整体呈减少趋势的标准误差(SE)表明代用资料的增多可以减少重建序列的不确定性。重建结果与其它研究给出的南极涛动指数有着较好的一致性。重建序列的功率谱分析表明,过去500多年南极涛动年际变率突出的周期有2.4a、2.6a、6.3a,年代际变率突出的周期有24.1a、37.6a,均达到95%显著性水平。进一步的小波分析表明南极涛动在不同时间尺度上的变率及其周期有着随时间而演变的特征。
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