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Search Results: 1 - 10 of 416576 matches for " 朱静 王皓 梁改芹 严自正 "
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焦曲霉产木聚糖酶的研究*
,,,
生物工程学报 , 1999,
Abstract: 从1144株真菌中筛选到一株产木聚糖酶活力较高的焦曲霉(Aspergillus ustus)。该菌株适宜的产酶条件为在4%麸皮液中添加0.5%葡萄糖,0.4%硝酸钠及0.1%氯化钠,30℃振荡培养6d,木聚糖酶活力可达2176 u/mL。酶反应的最适温度为55℃,最适pH为5.5,在pH5~8酶活力稳定。45℃保温1h,酶活力剩余35%。酶水解桦木木聚糖的Km值为4.3×10-3g/mL,Vmax值为4.9mg/(mL·min)。酶解产物以木三糖和木四糖为主,表明该酶是一种典型的内切糖苷酶。
焦曲霉产木聚糖酶的研究*

生物工程学报 , 1999,
Abstract: 从1144株真菌中筛选到一株产木聚糖酶活力较高的焦曲霉(aspergillusustus)。该菌株适宜的产酶条件为在4%麸皮液中添加0.5%葡萄糖,0.4%硝酸钠及0.1%氯化钠,30℃振荡培养6d,木聚糖酶活力可达2176u/ml。酶反应的最适温度为55℃,最适ph为5.5,在ph5~8酶活力稳定。45℃保温1h,酶活力剩余35%。酶水解桦木木聚糖的km值为4.3×10-3g/ml,vmax值为4.9mg/(ml·min)。酶解产物以木三糖和木四糖为主,表明该酶是一种典型的内切糖苷酶。
黑曲霉木聚糖酶的纯化与性质
陈红歌,,,,贾新成,张树政
菌物学报 , 2000,
Abstract: 由凝胶电泳酶谱检测到黑曲霉149发酵液中存在两型木聚糖酶,依次为X-Ⅰ和X-Ⅱ.通过硫酸铵分级沉淀及DEAE-SephadexA50柱层析分别将X-Ⅰ、X-Ⅱ纯化到凝胶电泳均一。由SDS一凝胶电泳和浓度梯度凝胶电泳测得X-Ⅰ和X-Ⅱ的分子量分别为37kDa,76kDa,24kDa和23kDa,X-Ⅰ具有亚基。二者的含糖量分别为276%和7.3%。X-Ⅰ和X-Ⅱ最适反应温度分别为50℃和55℃,pH为46和5.2。在pH4.6~9.2和pH4.0~10.0之间X-Ⅰ、X-Ⅱ活力稳定。50℃保温24h,X-Ⅰ活力仍为100%,而X-Ⅱ的活力已降为2.8%。HgCl2和AgNO3显著抑制X-Ⅰ、X-Ⅱ的活力。X-Ⅰ与X-Ⅱ水解不同来源的木聚糖,其产物有所不同。
Screening of acidic xylanase producing strain and studies on its enzyme production conditions]
酸性木聚糖酶产生菌的筛选及产酶条件

Chen Hongge,Zhu Jing,Liang Gaiqin,Yan Zizheng,Zhang Shuzheng,
陈红歌
,,,,张树政

微生物学报 , 1999,
Abstract: From 150 fungal strains, the authors found 8 strains contained mainly of xylanase activity over 100 U/mL in which the No. 149 strain was the highest xylanase producer. Which tentatively identified as Aspergillas niger. The appropriate medium composition was as follows: wheat bran hemicellulose 4%; NaNO3 1%; wheat bran 1% prepared in Mandels nutritional solution without (NH4)2SO4 and urea. After cultivated in shake-flask at 28 degrees C-32 degrees C for 60 h, the activity reached the highest value of 357.2 U/mL. The optimum pH of xylanase was 4.6 and it was stable at pH3-11. The fermented broth of strain 149 contained in addition to xylanase (relative activity 100) also included amylase(1.8), mannanase(0.98), beta-xylosidase(0.94) and cellulase(0.17).
PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER
黑曲霉木聚糖酶的纯化与性质

CHEN Hong-Ge,ZHU Jing,LIANG Gai-QinYAN Zi-Zheng,JIA Xin-Cheng,ZHANG Shu-Zheng,
陈红歌
,,,,贾新成,张树政

菌物学报 , 2000,
Abstract: 由凝胶电泳酶谱检测到黑曲霉149发酵液中存在两型木聚糖酶,依次为X-Ⅰ和X-Ⅱ.通过硫酸铵分级沉淀及DEAE-SephadexA50柱层析分别将X-Ⅰ、X-Ⅱ纯化到凝胶电泳均一。由SDS一凝胶电泳和浓度梯度凝胶电泳测得X-Ⅰ和X-Ⅱ的分子量分别为37kDa,76kDa,24kDa和23kDa,X-Ⅰ具有亚基。二者的含糖量分别为276%和7.3%。X-Ⅰ和X-Ⅱ最适反应温度分别为50℃和55℃,pH为46和5.2。在pH4.6~9.2和pH4.0~10.0之间X-Ⅰ、X-Ⅱ活力稳定。50℃保温24h,X-Ⅰ活力仍为100%,而X-Ⅱ的活力已降为2.8%。HgCl2和AgNO3显著抑制X-Ⅰ、X-Ⅱ的活力。X-Ⅰ与X-Ⅱ水解不同来源的木聚糖,其产物有所不同。
产碱菌麦芽四糖淀粉酶多型性的形成
车风琴 张树政
生物工程学报 , 1997,
Abstract: 产碱菌培养液的酶谱呈-主带g4a-l及两次带g4a-2与g4a-3,分别进行纯化并鉴定其水解产物,均为麦芽四糖.说明三者全是麦芽四塘淀粉酶,它们以多型性形式存在于产碱菌培养液中。培养液中没有检出塘苷酶。24,72,96h培养液中不含蛋白酶(仅48h培养液中有微量蛋白酶),说明多型性不是糖苷酶和蛋白酶造成的。改变培养条件不影响多型性产生.仅影响到g4a-2及g4a-3生成量的多少。
Studies on the Multiple forms of Maltotetraose forming Amylase from Alcaligenes sp.
产碱菌麦芽四糖淀粉酶多型性的形成

Zhu Jing,Chen Fengqin,Yan Zizheng,Liang Gaiqin,Zhang Shuzheng,
,车风琴,,,张树政

生物工程学报 , 1997,
Abstract: 产碱菌培养液的酶谱呈-主带G4A-l及两次带G4A-2与G4A-3,分别进行纯化并鉴定其水解产物,均为麦芽四糖.说明三者全是麦芽四塘淀粉酶,它们以多型性形式存在于产碱菌培养液中。培养液中没有检出塘苷酶。24,72,96h培养液中不含蛋白酶(仅48h培养液中有微量蛋白酶),说明多型性不是糖苷酶和蛋白酶造成的。改变培养条件不影响多型性产生.仅影响到G4A-2及G4A-3生成量的多少。
麦芽四糖淀粉酶的晶体培养及初步衍射研究
马建华,耀萍,韩毅,毕汝昌,,
生物物理学报 , 1997,
Abstract: 麦芽四糖淀粉酶是水解方式独特、其产物又具有较广泛应用价值的一种淀粉酶。应用悬滴汽相扩散法,用从产碱菌发酵培养液分离纯化得到的麦芽四糖淀粉酶成功地培养出了质量较好的单晶体。测得该种晶体属正交晶系,空间群为P212121,晶胞参数a=46.6A。,b=65.8A。,c=170.9A。,一个不对称单位含一个麦芽四糖淀粉酶分子。用MarResearchIP面探测器收集了一套分辨率高于2.7A。的衍射强度数据。
微生物产生的木聚糖酶的功能和应用

生物工程学报 , 1996,
Abstract: 术聚糖是一种异质多糖,主要由木糖和阿拉伯糖组成。微生物产生的木聚糖酶来源广泛,能将木聚糖水解为木寡糖和d-木糖。该酶具有极大的应用价值,如可用于纸浆的漂白以减少环境污染,也可将造纸工业及农业废料中的木聚糖转化为d-木糖。
Functions and Applications of Microbial Xylanase
微生物产生的木聚糖酶的功能和应用

Zhu Jing Yan Zizheng,
,

生物工程学报 , 1996,
Abstract: Xylans are heteropolymers consisting principally of xylose and arabinose. Mi-crobial xylanases occur in various microorganisms and can hydrolyze xylans into xy-looligosaccharides and D-xylose. The industrial applications of the microbial xylanases include mainly two aspects,one is the enhancing of pulp bleaching so as to reduce the environment pollution and the other is the conversion of xylan to D-xylose in the wastes of the paper-pulp industry and agriculture.
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