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Search Results: 1 - 10 of 259824 matches for " 张颖 "
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液相色谱串联质谱对掺假牛肉的鉴别及定量研究

- , 2017, DOI: 10.13982/j.mfst.1673-9078.2017.2.035
Abstract: 本文以差异蛋白质组学为理论研究基础,利用高效液相色谱串联质谱(HPLC-MS/MS)实现了对猪肉或鸡肉掺假的牛肉的定性鉴别及定量分析。首先采用高分辨质谱仪(nLC-QE)分别找出牛肉、猪肉和鸡肉的相对专属性多肽链,并对其进行二级扫描,得到了三个物种的特异性离子对,进一步利用HPLC-MS/MS的多反应监测模式(MRM)对掺杂猪肉或鸡肉的牛肉进行特异性离子对验证,分别选择了牛肉、猪肉和鸡肉的9、8和7条多肽进行定性研究;并与聚合酶链式反应(PCR)技术进行比对,得到的结果一致;此外将鸡肉和猪肉以0.5、1.0、5.0、10.0、25.0%的比例掺杂于牛肉中,各选择3组离子对进行定量分析,其各自的线性相关系数均大于0.99,最低定量限为0.5%。由此表明,液相色谱串联质谱的方法可以快速地对牛肉中的猪肉和鸡肉进行鉴别并能准确定量分析。
Differential proteomics was used as the basis for theoretical study, while high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was employed to identify and quantify beef adulterated with pork and chicken. First, polypeptides relatively specific to beef, pork, and chicken were identified using a high-resolution mass spectrometer (nLC-QE), followed by product ion scan to determine the specific ion pairs in the three types of meats. These specific ion pairs were then detected in the beef adulterated with pork or chicken using HPLC-MS/MS in multiple reaction monitoring (MRM) mode, and a qualitative study was carried out using nine, eight, and seven quantitative peptides from beef, pork, and chicken, respectively. The results were compared with those obtained from polymerase chain reaction (PCR), and the results were found to be consistent with each other. Chicken and pork were blended in beef in proportions of 0.5, 1.0, 5.0, 10.0, and 25.0%, and three quantitative peptides specific to each type of meat were selected to perform the quantitative analysis. The linear correlation coefficient for each quantitative ion pair was more than 0.99, and the limit of quantitation was 0.5% blending. The results showed that the HPLC-MS/MS method can quickly and efficiently identify pork and chicken in beef, and can be used for accurate quantitative analysis.
电针结合特定体位治疗后半规管耳石症的疗效观察

- , 2017,
Abstract:
提高电脑文字输入速度的尝试

中国科技期刊研究 , 2001,
Abstract: 在编辑部工作中,提高电脑字、符输入速度十分必要。对“UCDOS”操作系统及“北大方正书版系统”辅助工具进行开发,结合编辑部工作电脑文字输入的需要,编“自定义词(条)”、创建“辅助码表”、增编“动态键盘”,以达到迅速、准确地输入文字(符号),从一个方面提高编辑部工作效率。
编辑在稿件科学性控制方面的责任与作为

中国科技期刊研究 , 2010,
Abstract:
The Signal Processing Design of the Spacial Transient Optical Signal Based on LabVIEW
基于LabVIEW空间瞬态光信号处理技术


光子学报 , 2006,
Abstract: The frequency features of the optical transient signal, the background signal and high frequency noise are analyzed, and the virtual instruments of signal processing are given based on LabVIEW. The design of the digital filter is introduced and the original signal is filtered, and then the spectrum estimation of the filtered signal gives the power spectrum density. The test result shows that the new method is simple, reliability and effective.
关于铁电铁弹材料的自然构形

力学学报 , 2000, DOI: 10.6052/0459-1879-2000-2-1997-311
Abstract:
黑龙江大兴安岭森林绿色核算研究

自然资源学报 , 2006, DOI: 10.11849/zrzyxb.2006.05.006
Abstract: ?在对国内外森林绿色核算研究综述的基础上,对黑龙江大兴安岭1997—2003年森林资源的实物量、价值量和森林绿色GDP等进行了核算。核算结果表明:黑龙江大兴安岭森林的eaGDP(environmentallyadjustedGDP)1997年为38.19×108元,2003年为57.34×108元,年均增长7.01%;森林的eaNDP(environmentallyadjustedNDP)1997年为25.70×108元,2003年为43.96×108元,年均增长9.36%。在森林绿色GDP核算的基础上,进行了资产负债和财富核算。结果表明,1997—2003年,黑龙江大兴安岭林地、林木存量价值呈减少的趋势;森林环境效益呈增加的趋势,反映在社会经济发展中对林地、林木存量的过度开发和利用。森林绿色GDP占GDP的比重和森林资源占国民财富的比重也呈下降的趋势,说明黑龙江大兴安岭虽然在天然林保护工程后,全面禁止天然林采伐,森林的生长量大于消耗量,表现为流量价值有所增加,反映森林是可持续的经营,但从存量上看对国民经济发展的支撑力是下降的。最后,研究指出,应加强生态补偿政策的研究,积极落实森林可持续经营对策和建议加强绿色财富政策的制定,以保证黑龙江大兴安岭森林的可持续发展和绿色财富的增长。
如何提升商业银行研发管理效率——基于经济学的分析

中央财经大学学报 , 2014,
Abstract: ?尽管我国商业银行已经搭建起系统性的研发管理架构,但管理机制运行不畅一直制约着研发效率的提升。笔者通过建立理论模型,对商业银行研发流程中各责任主体的行为进行经济学分析,并在行为分析的基础上,将影响研发管理效率的因素总结为信息成本和博弈成本两大类。最后,以模型分析为基础,针对商业银行如何提升研发管理效率提出改进建议。
青蒿素的提取工艺优化研究

北方园艺 , 2014,
Abstract: 以干青蒿叶粉末为原料,以青蒿素提取量为考察指标,采用有机溶剂提取法,以料液比、温度、时间为考察因素,在单因素试验基础上进行了3因素3水平正交实验,研究提取青蒿素的最佳工艺条件。结果表明青蒿素的最佳提取条件为料液比1∶20g/mL,提取温度50℃,提取时间2.5h,青蒿素提取量达5.27mg/g。
?河源市旅游产业与人才资源耦合发展研究

惠州学院学报 , 2014,
Abstract:
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