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Search Results: 1 - 10 of 257317 matches for " 张积森 "
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葡萄糖神经酰胺合酶基因在多药耐药肿瘤细胞株kbv200的表达及其与肿瘤多药耐药性的关系
杨泉,,,
南方医科大学学报 , 2004,
Abstract: 目的观察葡萄糖神经酰胺合酶(glucosylcerarnidesynthase,gcs)基因在人口腔表皮样癌多药耐药细胞株kbv200的表达及其与肿瘤多药耐药性(mdr)的关系。方法采用rt-pcr分析kbv200及其亲本kb细胞株gcs基因表达的差异,运用mtt法分析kbv200经糖脂合成酶抑制剂苯基棕榈酰胺吗啡丙醇(dl-ppmp)及钙离子通道阻滞剂异搏定处理前后细胞mdr的变化,应用rt-pcr技术检测kbv200细胞耐药逆转后gcs及mdrl基因的表达。结果kbv200细胞gcs和mdrl基因的表达明显强于kb细胞,而kb细胞mdrl基因表达为阴性。5~25μmol/ldl-ppmp均可抑制kbv200gcsmrna表达,25μmol/l时抑制强度最大;10μmol/l异搏定即可抑制kbv200gcsmrna表达,15μmol/l时抑制作用明显。dl-ppmp和异搏定均可抑制mdrl基因的表达,kbv200经25μmol/ldl-ppmp作用48h后细胞内mdrl表达为阴性。结论异搏定和dl-ppmp对gcs和marl基因表达的抑制调节呈浓度依赖性,它们可通过抑制gcs和mdrl基因表达,逆转kbv200对长春新碱的耐药。kbv200gcs基因的表达与mdr存在正相关,gcs基因可能在肿瘤的多药耐药过程中起着重要作用。
盐酸洛拉曲克长循环脂质体制备及其抑瘤特性研究
陈斯泽汪
南方医科大学学报 , 2008,
Abstract:
醋酸棉酚对人宫颈癌细胞增殖的影响及其机制初探
田丹,,,肖明星,,梁璐
解放军医学杂志 , 2008,
Abstract:
斑茅两个看家基因片段的克隆及其在基因芯片中的应用
,李伟,阙友雄,阮妙鸿,木清*,陈如凯?
热带亚热带植物学报 , 2007, DOI: 10.3969/j.issn.1005-3395.2007.4.001
Abstract: 根据已发表的同源基因序列,利用rt-pcr技术分离了斑茅(erianthusarundinaceus)的gapdh和aprt两个看家基因片段,用它们作为cdna芯片阳性参照,以未经聚乙二醇(peg)胁迫处理的斑茅叶片为对照,和peg胁迫的4组材料同cdna芯片进行杂交分析。杂交结果显示,gapdh杂交的cy5与cy3平均信噪比(signal/noise,s/n)分别为56.12和60.8,aprt杂交的cy5与cy3平均信噪比分别为51.06和47.25,信噪比均很高;同时两个看家基因的杂交都显示出极强的信号,其中gapdh的杂交信号值大于10000,aprt也在8000以上,杂交结果可靠。分析了peg胁迫下4个时段badh与两个看家基因的表达,badh的表达有明显变化,而看家基因表达均较稳定。上述结果表明所克隆的两个看家基因在斑茅中表达量高,且peg胁迫下表达较为稳定,是基因芯片理想的阳性参照。
肝动脉栓塞化疗联合氩氦刀消融和无水乙醇注射治疗肝癌
朱伟良李虹义健汪
南方医科大学学报 , 2009,
Abstract:
nk/lak细胞对人口腔癌耐细胞药株杀伤活性的观察
郑颖娟,,,
南方医科大学学报 , 2004,
Abstract: 目的明确免疫活性细胞对肿瘤细胞的杀伤活性是否与肿瘤的耐药性相关。方法采用连续形态学观察及mtt比色法,研究淋巴因子激活的杀伤(lak)细胞及自然杀伤(nk)细胞,对人口腔癌耐药细胞株kbv200耐药性逆转前后及亲本敏感株kb的杀伤活性。(1)在肿瘤细胞与lak细胞共育后3h内连续在倒置显微镜下观察lak细胞对上述3者的杀瘤效应;(2)采用mtt比色法检测nk及lak对3株细胞的杀伤率。结果与kb细胞组相比,在kbv200和kbv200+维拉帕米组,lak细胞出现在靶细胞周围的时间早、数量多,伸出伪足的lak细胞比率高,出现集落样细胞团块时间亦早。nk、lak细胞对kbv200细胞株耐药性逆转前后的杀伤率均明显高于敏感株kb(p<0.05),而对耐药株耐药性逆转前后的杀伤率无统计学差异(p>0.05);lak对各株的杀伤活力均明显强于nk细胞(p<0.05)。结论免疫活性细胞对kbv200细胞株有较强的杀伤作用,逆转耐药性不降低免疫活性细胞杀伤活力,提示细胞过继免疫治疗可能成为控制化疗耐药病人病情发展的一个有效手段。
多处损伤壁板的剩余强度研究
李仲,,广,肖迎春,白生宝
工程力学 , 2009,
Abstract: 多处损伤(MSD)严重危害老龄飞机的结构完整性。进行了多处损伤铝合金壁板的剩余强度试验研究。试验件包括加筋板和平板两种壁板,含有不同尺寸的主裂纹和MSD裂纹,裂纹为钼丝切割预制的穿透裂纹。壁板承受拉伸载荷直到破坏,通过载荷传感器和计算机记录破坏载荷。以试验数据为基础,提出了一种改进的评估多处损伤壁板剩余强度模型。用塑性区连通准则和提出的改进剩余强度模型分别计算了多处损伤壁板的剩余强度。与试验结果的比较表明:对不同的主裂纹长度、MSD裂纹长度,以及不同的主裂纹与相邻的MSD裂纹之间的韧带长度b,塑性区连通准则和改进的剩余强度模型预测结果的最大误差分别为53.63%和23.08%,而平均误差则分别为20.74%和8.6%。可以看出,对不同的裂纹尺寸,改进的剩余强度模型大大提高了预测结果的精度,可以直接应用于工程实践。
卵巢癌顺铂耐药细胞中dna转录与修复相关基因的表达
曹漫明,,,胡喜钢,胡利娟
南方医科大学学报 , 2005,
Abstract: 目的研究dna转录与修复相关基因的表达与卵巢癌顺铂耐药性之间的关系。方法以卵巢癌亲本细胞系coc1为对照,应用cdna微阵列检测其顺铂耐药细胞系coc1/ddp中dna转录与修复相关基因的表达。结果和结论与coc1细胞相比,coc1/ddp细胞有143个基因的表达水平发生改变,其中共有20个dna转录与修复相关基因的表达发生改变,表达上调的有13个基因,表达下调的有7个基因,包括cad、ddb1、ddit4等大量dna修复基因以及一般转录因子rna多聚酶ⅰ、ⅱ基因和dhx9、ddx3x基因的表达上调以及mlh1和btf3基因的低表达,提示coc1/ddp细胞对顺铂的耐药性与dna转录和损伤修复能力的异常有关。
改良缝合法治疗急性闭合性跟腱断裂的疗效分析
Effectiveness of modified suture technique in treatment of acute closed Achilles tendon rupture

詹俊锋,方家刘,,程文丹,吕浩,荆珏华
- , 2017, DOI: 10.7507/1002-1892.201702090
Abstract: 目的探讨使用改良缝合法治疗急性闭合性跟腱断裂的临床疗效。 方法2013 年 12 月—2016 年 12 月,采用改良缝合法治疗 29 例急性闭合性跟腱断裂。男 23 例,女 6 例;年龄 22~45 岁,平均 34 岁。患者均为运动损伤,其中左侧 22 例、右侧 7 例。术前美国足踝外科协会(AOFAS)踝与后足功能评分为(44.6±3.6)分;参照 Amer-Lindholm 疗效评定标准评价均为差。MRI 检查显示跟腱完全断裂。 结果手术时间 35~62 min,平均 46 min;术中出血量 7~15 mL,平均 10 mL。术后切口均Ⅰ期愈合,无腓肠神经损伤发生。患者均获随访,随访时间 5~38 个月,平均 18.4 个月。术后 3 个月,AOFAS 踝与后足功能评分为(93.1±4.3)分,与术前比较差异有统计学意义( t=–49.581, P=0.000)。参照 Amer-Lindholm 疗效评定标准评价,获优 24 例、良 5 例,优良率 100%;与术前比较差异有统计学意义( Z=–7.294, P=0.000)。MRI 检查示跟腱连续性完整。随访期间均无跟腱再断裂发生。 结论改良缝合法治疗急性闭合性跟腱断裂具有创伤小、再断裂发生率低、腓肠神经损伤风险低及功能恢复较好等优点。
ObjectiveTo investigate the effectiveness of modified suture technique in the treatment of acute closed Achilles tendon rupture. MethodsBetween December 2013 and December 2016, 29 cases with acute closed Achilles tendon rupture were treated with modified suture technique. There were 23 males and 6 females with an average age of 34 years (range, 22-45 years). All patients were sport injuries. The injury located at left side in 22 cases and right side in 7 cases. American Foot and Ankle Surgery Association (AOFAS) ankle and hind foot function score was 44.6±3.6. According to the Amer-Lindholm criteria, 29 cases were rated as poor. MRI showed the complete rupture of the Achilles tendon. ResultsThe operation time was 35-62 minutes (mean, 46 minutes). The intraoperative blood loss volume was 7-15 mL (mean, 10 mL). The incisions healed by first intention, and no sural nerve injury occured. All patients were followed up 5-38 months (mean, 18.4 months). AOFAS ankle and hind foot function score was 93.1±4.3 at 3 months after operation, showing significant difference when compared with the preoperative value ( t=–49.581, P=0.000). According to the Amer-Lindholm criteria, the results were excellent in 24 cases and good in 5 cases, and the excellent and good rate was 100%; there was significant difference between pre- and post-operation ( Z=–7.294, P=0.000). MRI showed the continuous and integrity of Achilles tendon. No Achilles tendon rupture recurred during the follow-up period. ConclusionModified suture technique in the treatment of acute closed Achilles tendon rupture owns the advantages of less trauma, lower recurrence rate of rupture, lower risk of the sural nerve injury, and satisfactory function recovery.
游离上臂外侧分叶皮瓣修复手部多创面皮肤缺损的临床应用
CLINICAL APPLICATION OF LATERAL ARM LOBULATED FLAPS TO REPAIR MULTIPLE SOFT TISSUE DEFECT OF HAND

叶曙明,滕晓峰,陈宏,荆珏华,
- , 2016, DOI: 10.7507/1002-1892.20160089
Abstract: 目的探讨应用游离上臂外侧分叶皮瓣修复手部多创面皮肤软组织缺损的方法和临床效果。 方法2013年10月-2015年9月,采用游离上臂外侧分叶皮瓣修复手部外伤后多处皮肤软组织缺损13例。患者均为男性,年龄23~45岁,平均28岁。致伤原因:工业贯穿伤7例,交通事故伤3例,热压伤3例。其中6例外伤后手指毁损行开放性截指后遗留2指皮肤缺损,7例外伤后手掌伴手背皮肤缺损。皮肤缺损范围6 cm×5 cm~9 cm×6 cm。所有患者急诊清创,二期修复,受伤至手术时间5~9 d,平均7 d。皮瓣切取范围6 cm×5 cm~9 cm×6 cm。 结果术后皮瓣完全成活,无血管危象等并发症发生。患者供受区切口均Ⅰ期愈合,植皮全部成活。13例均获随访,随访时间3~24个月,平均12个月。皮瓣质地、颜色与周围正常软组织相似;4例皮瓣稍臃肿,于术后3个月行皮瓣修整术。所有皮瓣感觉恢复至S 3~S 4。 结论以桡侧血管束为蒂的游离上臂外侧分叶皮瓣血管解剖恒定,切取简便,分叶后旋转弧度较大,皮瓣质地和感觉恢复好,是修复手部外伤后中、小面积多创面皮肤缺损的一种有效方法。
ObjectiveTo explore the effectiveness and operation of repairing multiple soft tissue defects in hands with lateral arm lobulated flaps. MethodBetween October 2013 and September 2015, 13 cases of multiple soft tissue defects in the hand with tendon or bone exposure were treated with lateral arm lobulated flaps. All patients were males with average age of 28 years (range, 23-45 years). Defects were caused by penetrating injury in 7 cases, traffic accident injury in 3 cases, and hot-crush injury in 3 cases. Six patients had skin defect of the left 2 fingers after opening finger amputation, and 7 patients had skin defect of the palm and the back after hand injury. The size of skin defects ranged from 6 cm×5 cm to 9 cm×6 cm. All patients underwent emergency debridement and two-stage repair; the duration from injury to operation was 5-9 days (mean, 7 days). The size of flap was 6 cm×5 cm-9 cm×6 cm. ResultsAll flaps survived completely, with no vascular crisis. Primary healing was obtained at donor and recipient sites, and the grafted skin survived. All cases were followed up 3-24 months (mean, 12 months). The appearance and texture of the flaps were similar to those of adjacent skin. Bulky flap was observed in 4 cases, and second stage operation was performed to make the flap thinner at 3 months after operation. The sensation of flap reached S 3-S 4. ConclusionsThe lateral arm lobulated flap based on the radial collateral artery has constant vascular anatomy, easy-to-harvest, and large rotation angle. It is an effective procedure to repair small and medium size skin defects of the hand with satisfied texture and sensory recovery.
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