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Search Results: 1 - 10 of 431745 matches for " 多齿新米虾(Neocaridina denticulate);药物残留;麸胱苷肽硫基转移酶(Glutathione-S-Transferases);单氧酶(Monooxygenase);GOT (Glutamic Oxaloacetic Transaminase);GPT (Glutamic Pyruvic Transaminase)<br>Neocaridina denticulate "
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人类常用止痛剂布洛芬(Ibuprofen)及乙酰胺酚(Acetaminophen)对多齿新米虾(Neocaridina denticulate)的影响
Effect of Drug Residues of Ibuprofen and Acetaminophen on Aquatic Neocaridina denticulate
 [PDF]

赖慧绮, 蔡宜君, 叶芳伶, 宋宏红, 陈健民, 黄大骏
International Journal of Ecology (IJE) , 2012, DOI: 10.12677/ije.2012.12004
Abstract:

近年来许多研究指出,人类长期使用的药物如抗生素或止痛剂等医疗药品,无法经由污水处理厂而完全分解消失,当这些药物进入环境中时将可能具有生物毒性,并对生态环境系统造成冲击。为了解人类常用止痛剂(ibuprofen, IBU)及乙酰胺酚(acetaminophen, ACE)残留于水体后对水生生物可能的影响。本研究应用多齿新米虾(Neocaridina denticulate,后简称米虾)进行0.115 mg/LIBUACE不同浓度的曝露。并于曝露后147天,并测定米虾体内解毒酵素的活性(monooxygenase, Mon)glutathione-S-transferase, GST)与肝胰脏受损指标(glutamic oxaloacetic transaminase, GOT)(glutamic pyruvic transaminase, GPT)。试验结果显示,米虾曝露IBUACE147天,各处理组间Mon
以短暂暴露于邻苯二甲酸二丙酯(Dipropyl Phthalate)之淡水多齿新米虾(Neocaridina denticulata)建立生态毒理基因组生物标志物
Development of Ecotoxicogenomic Biomarkers on the Freshwater Shrimp (Neocaridina denticulate) Following Short-Term Exposure to Dipropyl Phthalate
 [PDF]

蔡伊茜, 宋宏红
International Journal of Ecology (IJE) , 2013, DOI: 10.12677/IJE.2013.24007
Abstract:
邻苯二甲酸酯类化合物(phthalate esters; PAEs)是广泛用于塑料制品之塑化剂和造成环境污染甚为可观的內分泌干擾物質。本研究以多齿新米虾(Neocaridina denticulata)暴露于次致死剂量(50 mg/L)之一種PAE——邻苯二甲酸二丙酯(dipropyl phthalate; DPrP)1天,利用抑制性扣减杂交法建立一套基因组指标,探讨其全面基因的表现变化。实验中共筛得71个独特的表达序列标签(expressed sequence tages; ESTs),包括23ESTs对应为已知功能基因及48ESTs为未知功能。根据生理功能,已知功能基因分别与9类相关,包括代谢、呼吸、防御、核糖体、染色体、转译、传讯、视觉与结构。虾子暴露于高浓度DPrP (50 mg/L)时,3个免疫及代谢相关的基因和3个未知功能基因,共6个基因的mRNA表现量明显下降。暴露于非致死剂量1.0 mg/L时,共8个基因受到影响,包括正调节的4个已知功能和1个未知功能基因,以及3未知功能基因被负调控。虾子暴露于更低剂量0.5 mg/L
Modeling analysis of GST (glutathione-S-transferases) from Wuchereria bancrofti and Brugia malayi
Rayavarapu Bhargavi,Siddharth Vishwakarma,Upadhyayula Suryanarayana Murty
Bioinformation , 2005,
Abstract: GST (glutathione S-transferases) are a family of detoxification enzymes that catalyze the conjugation of reduced GSH (glutathione) to xenobiotic (endogenous electrophilic) compounds. GST from Wb (Wuchereria bancrofti) and Bm (Brugia malayi) are significantly different from human GST in sequence and structure. Thus, Wb-GST and Bm-GST are potential chemotherapeutic targets for anti-filarial treatment. Comparison of modeled Wb and Bm GST with human GST show structural difference between them. Analysis of the active site residues for the binding of electrophilic co-substrates provides insight towards the design of parasite specific GST inhibitors.
EXPRESSION AND ACTIVITY ANALYSIS OF THE HUMAN GLUTATHIONE-S-TRANSFERASE IN LACTOCOCCUS LACTIS
人谷胱甘肽硫转移酶A1在乳酸乳球菌中的表达及活性研究

Xiang Hua,Xhang Yiqing,Wei Wenzhong,Tan Huarong,
向华
,张亦清,卫文仲,谭华荣

微生物学报 , 2000,
Abstract: Theglutathione S transferaseA1cDNAwasamplifiedfromhumanlivertotal RNAsbyRT PCRandwasclonedintoaEscherichiacoliexpressionvectorpET2 3b ,then therecombinantplasmidpET2 3bhgstwasintroducedintoE .coliBL2 1 (DE3)andinduced byIPTG ,thehigh levelexpressionofhGSTA1appearedintheE .colicells.ThecDNAen codinghGSTA1wassubclonedintopMG36e,alactococcalexpressionvector,andintroduced intoLactococcuslactisMG1 36 3byelectroporation .Inthepositivetransformants,thehGS TA1wasexpressedasafusionproteinwhichwasverifiedbySDS PAGEandWesternblot. ThehGSTA1frombothE .coliandL .lactiswaspurifiedbyaffinitychromatographyon glutathione agaroseandallshowedenzymaticactivity .Thepotentialapplicationofthere combinantLactococcuslactisinfunctionalfoodwasalsodisscussed .
Purification and properties of recombinant GST-haparinase III and optimizationm of cultivation conditions
重组黄杆菌肝素酶III的纯化、表征及培养条件对酶生产的影响

Xing Gao,Jian Zhao,Liqiang Fan,Suxia Li,Fujun Wang,Shengli Ji,Qinsheng Yuan,
高兴
,赵健,范立强,李素霞,王富军,姬胜利,袁勤生

生物工程学报 , 2009,
Abstract: 肝素酶III是一种特异性地裂解乙酰肝素的酶,在大肠杆菌中表达时容易形成包涵体。为实现肝素酶III的可溶性表达,利用谷胱甘肽-S-转移酶(GST)与肝素酶III融合性能,通过构建相应的表达质粒pGEX-heparinaseIII,在大肠杆菌中实现了肝素酶Ⅲ的可溶性表达。粗酶通过一步亲和纯化其纯度可达95%以上。通过对LB培养基摇瓶培养Escherichia coli BL21的诱导时机、诱导剂用量、诱导时间等培养条件的优化,确定了该可溶性肝素酶III融合蛋白的最适生产条件。通过对纯酶的最适反应温度、pH、Ca2+浓度等一系列性质研究,确定了该酶的最适反应条件。
Effcts of the herbicide gallant and mercury on liver function of Tilapia zilIi
Abdel-Salam Mohamed Ibraik Ohaida
Egyptian Journal of Hospital Medicine , 2008,
Abstract: Background: Herbicides are highly toxic for both human and animal health. The increased application of herbicides in agriculture during the last decades has resulted in the contamination of both soil and water. Also do heavy metals, which represent one of the most important group of pollutants produced as a result of many industrial activities which can find their way easily to the normal aquatic environments, disturbing and damaging the existing organisms. Material and Methods: Fishes of the species Tilapia zillii were exposed to sublethal concentration of herbicide Gallant (haloxyfop-ethopxy ethanol ester) (3 mg/L), mercury as mercuric chloride (6 mg/L) and a combined dose of herbicide (1.5 mg/L) and mercury (3 mg/L) for 96 hrs., in aquaria under controlled laboratory conditions. A comparative physiological study was carried out to test the toxicological effects of these pollutants on glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) activities & hepatosomatic index of liver. Results: A progressive decrease in enzyme activity as well as total protein content of liver were observed, while hepatosomatic index showed a slight insignificant increase
亚砷酸钠对神经细胞活力及3MST蛋白表达的影响
Effects of sodium arsenite on cell viability and expression of 3MST in neuronal cells

王念,潘际刚,范海琼,聂杨,孙达权,徐国强
- , 2017,
Abstract: 目的 探讨亚砷酸钠(NaAsO??2)对PC12细胞的生长和3-巯基丙酮酸硫基转移酶(3MST)表达的影响。方法 PC12细胞分别暴露于20、40、60、80、100μmol?L??-1?? NaAsO??212、24、36和48小时。倒置显微镜观察PC12细胞在药物处理后的形态变化。MTT法测定NaAsO??2在不同时间、不同浓度对PC12细胞存活率的影响;Western blot检测PC12细胞3MST的表达。结果 亚砷酸钠引起细胞缩小、胞体变圆、细胞间隙变宽和死细胞增多等显著细胞形态学改变。40-100μmol?L??-1??亚砷酸钠可以明显降低PC12 细胞的存活率;3MST蛋白表达也显著减少,呈现剂量和时间依赖效应(P<0.05)。结论 亚砷酸钠可抑制PC12细胞生长、诱导细胞损伤,其作用机制可能与下调3MST的表达有关。
Oxidation Stress and Toxicity of TBBPA Pollution on Polychaete Tubifex (Monopylephorus limosus)
四溴双酚-A污染对颤蚓的氧化胁迫及毒性

LI Ya-ning,ZHOU Qi-xing,HU Xian-gang,LUO Yi,
李亚宁
,周启星,胡献刚,罗义

环境科学 , 2008,
Abstract: Using the indoor simulating method of dynamic and static exposure respectively, the toxic effects of TBBPA on the antioxidant enzyme defense systems and Glutathione-S-transferase (GST) activity of tubifex Monopylephorus limosus were examined. The activity of superoxide dismutase (SOD) and catalase (CAT) with time was also examined. The results showed that after an 8 d exposure, the SOD activity was enhanced at first and then inhibited gradually, at last enhanced again. The highest activity of SOD (p < 0.01) was examined under 0.05 mg/L concentration of TBBPA. And the activity of SOD was much higher than that of control (1.5-7.8 times more than that of the control). The activity of CAT showed a tendency of induction firstly and then inhibition, then induction again and at last inhibition, reached the highest value under 0.5 mg/L of TBBPA. Furthermore, the CAT activity was higher than that of the control (1.1-1.9 times more than that of the control) except that under 0.005 mg/L and 0.25 mg/L of TBBPA. Moreover, the highest activity of GST (p < 0.01) was observed under 0.25 mg/L of TBBPA. The activity of GST was enhanced gradually at first and then inhibited. As the same as SOD, the activity of GST was induced significantly (p < 0.05). The changes in the SOD activity showed an "M" trend,while that in the CAT activity showed an "N" trend. And the activity of SOD is steadier than that of CAT. Thus, changes in the activity of SOD and GST, especially SOD, can better reflect the toxic effects of pollutants on tubifex.
A molecular epidemiological study on the relationship between the polymorphism of GSTP1 and susceptibility to gastric cancer in northern Chinese
中国北方人群谷胱甘肽转硫酶P1基因多态性及其与胃癌遗传易感性的关系

ZHANG Ye,SUN Li-Ping,CHEN Wei,BAI Xue-Lei,LIU Jin,WANG Xu-Guang,LIU Yun-Peng,YUAN Yuan,
张晔
,孙丽萍,陈威,白雪蕾,刘谨,王旭光,刘云鹏,袁媛

遗传 , 2007,
Abstract: We analyzed the distribution of glutathione-S-transferase P1 gene(GSTP1) polymorphism in a population from northern China and the relationship between the polymorphic BsmAI site in its exon 5 and gastric cancer susceptibility and evaluated the combined effect of GSTP1 polymorphism and H.pylori infection on gastric cancer.Blood samples were taken from 1,612 subjects in areas of high and low incidence of gastric cancer.Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) were performed to analyze the genotype of a GSTP1 polymorphism in exon 5(Ile105Val) .Serum levels of anti-H.pylori IgG were measured by enzymed-linked immunosorbent assay.We found that the GSTP1 Val variant allele frequency was 22%,which was significantly different from the Western people.There was a significant difference in GSTP1 allele gene distribution between the area of high incidence of gastric cancer(23%) and low incidence(20%) .The frequency of GSTP1 Val/Val genotype was statistically higher in the gastric cancer group compared tothe non-gastric cancer population.Analysis showed a statistically significant 1.587-fold increase in gastric cancer risk associated with the GSTP1 Val allele.Moreover,there was a statistically significant interaction(odds ratio,17.571;95% confidence interval,6.207 ? 49.742) between GSTP1 Val/Val genotype and positive H.pylori IgG status.Our results indicate that the distribution of GSTP1 polymorphism has geographic differences.Individuals with the GSTP1 Val allele gene show an increased risk for gastric cancer.Association of the GSTP1(Val/Val) genotype with H.pylori IgG positive status could significantly increase gastric cancer risk.
细菌群体感应淬灭酶的研究进展*
邱健,贾振华,李承光,马宏,宋水山
微生物学通报 , 2006,
Abstract: 细菌的群体感应系统(Quorum sensing,QS)参与许多生物学功能的调控,其中包括动植物病原细菌致病因子的生成以及人类某些病原细菌生物膜的形成。酰基高丝氨酸内酯(N-acylhomoserine lactone,AHL)是调控群体感应系统的关键信号分子。近年的研究表明,不同生物体包括细菌和真核生物中都存在类别不同的能够降解AHL的群体感应淬灭酶(Quorum-quenching enzyme)。在AHL依赖型致病菌和转基因植物中表达AHL降解酶能有效地抑制QS信号分子的积累,从而阻断了病原细菌的发
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