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Search Results: 1 - 10 of 30632 matches for " 唐赟 "
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一株高效降解中长链烷烃菌株的分离鉴定及其降解特性研究
Isolation and Characterization of a Novel Medium-Long Alkane-Degrading Strain
 [PDF]

, 谭洪
Bioprocess (BP) , 2013, DOI: 10.12677/BP.2013.31002
Abstract: 从南充市炼油厂被石油污染土壤中分离到一株中长链烷烃降解菌TY12。采用形态学观察、生理生化指标测定、抗生素抗性实验、16S rRNA基因序列同源性分析等多种方法对该菌株进行鉴定。菌株为Acinetobacter beijerinckii,革兰氏阴性,短杆状,无荚膜,无芽孢,接触酶阳性,氧化酶阴性,菌体大小为0.9~1.6 μm × 1.5~2.5 μm;对新霉素、氯霉素、卡拉霉素等14种抗生素敏感,而对四环素、阿莫西林、氨苄西林耐药;在GenBank中与其16S rRNA基因序列相似度最高的模式株分别为Acinetobacter beijerinckii CCM7266 (AJ626712)、Acinetobacter beijerinckii LMH6214 (AJ303013),相似性为100%。研究表明,以工业乙醇为唯一碳源的无机盐培养基培养种子最适宜;其最适温度和pH分别为30℃和7.0,无水乙醇最适加入量为0.7%,最适酵母粉浓度为0.025 g/L。在初始正十二烷浓度为1%(W/V)培养基中接入1%种子液,于30℃、180 r/min震荡培养36 h,正十二烷降解率可达92%;菌株对正构烷烃的降解范围较宽,能以C12~C32的正构烷烃为唯一碳源和能源生长。该菌是一株能够降解中长链烷烃的不动杆菌新种,具有应用到石油污染修复的潜力。
A strain of medium-long chain n-alkanes degrading strain TY12 was screened from petroleum-contaminated soils of Nanchong oil refinery. We adopted several phenotypic and genotypical methods, such as morphological, physical and biochemical characteristics, antibiotic susceptivities, 16S rRNA gene sequences based phylogenetic analysis, to outline the basic biological characteristics and determinate the phylogenetic position. The Gram-negative isolate TY12 was a member of the genus Acinetobacter, short rods, no capsules, no endospores, catalase-positive, oxidase-negative, 0.9 - 1.6 μm in diameter and 1.5 - 2.5 μm in length; TY12 was susceptible to 14 kinds of tested antibiotics but resistant to Tetracycline, Amoxicillin, Ampicillin; The similarity between its 16S rRNA gene and that of its most closely related type strain in GenBank Acinetobacter beijerinckii CCM7266 (AJ626712), Acinetobacter beijerinckii LΜH6214 (AJ303013) were 100%. The optimal temperature and pH for the strain utilizing industrial ethanol were 30?C and 7.0, and the optimal concentration of ethanol and concentration of yeast extract were 0.7% and 0.025 g/L, respectively. The n-dodecane degradation was 92% after the strain was growing on in hydrocarbon degradation medium with 1% (W/V) of n-dodecane at 30?C and 180 r/min for 36 h. The strain could degrade a large range of n-alkanes with chain length C12 - C32. It has potential in bioremediation of oil contaminated environment.
不同金属离子对苯酚降解菌XH-10的生长和苯酚降解的影响
The Affect of Growth and Phenol Degrading on Phenol Degrading Strain XH-10 with Different Metal Ions
 [PDF]

, 岳黎, 彭超, 宋嫣, 谭洪
Bioprocess (BP) , 2012, DOI: 10.12677/bp.2012.21006
Abstract: 本文探测了18种金属离子对苯酚降解菌XH-10生长和苯酚降解的影响。结果显示:Al3+、Co2+、Cu2+、Hg2+、Ni2+、Ag+对苯酚降解菌XH-10的生长和苯酚降解有强烈的抑制作用;K+、Zn2+、Cr2+、Mo2+、Pb2+、Sn2+对苯酚降解菌XH-10的生长和苯酚降解有一定的抑制作用,但在低浓度时,XH-10有一定的生长和苯酚降解潜力;Ca2+、Mg2+、Na+、Mn2+在较低浓度时对苯酚降解菌XH-10的生长有促进作用,对苯酚降解的抑制作用较小,在高浓度时对菌体生长和苯酚降解的抑制作用明显;Ba2+对苯酚降解的抑制作用明显,而在较低浓度时对苯酚降解菌XH-10的生长有促进作用;Fe3+对菌体生长的抑制作用明显,而在较低浓度时对苯酚降解菌XH-10的苯酚降解抑制作用较小。其研究结果对菌株XH-10用于含酚废水的处理提供了一定的依据。
This article surveyed the influence which 18 metal ions on phenol degrading bacterium XH-10 growth and the phenol degradation. The results showed: Al3+, Co2+, Cu2+, Hg2+, Ni2+, Ag+ have intense inhibitory action to the phenol degrading bacterium XH-10 growth and the phenol degeneration; K+, Zn2+, Cr2+, Mo2+, Pb2+, Sn2+ have certain in-hibitory action to the phenol degrading bacterium XH-10 growth and the phenol degeneration, but at low concentrations, XH-10 has certain growth and the phenol degeneration potential; Ca2+, Mg2+, Na+, Mn2+ at lower concentration to the phenol degrading bacterium XH-10 growth have promoter action, degrades the inhibitory action to be small, at high concentrations to the bacterium growth and the phenol degradation have obvious inhibitory; Ba2+ the inhibitory action which degrades to the phenol is obvious, but at lower concentration have promoter action to the phenol degrading bac-terium XH-10 growth; Fe3+ the inhibitory action which growth to the bacterium is obvious, but at lower concentration have smaller inhibitory action to the phenol degrading bacterium XH-10 phenol degeneration. The results of the phenol degrading bacterium XH-10 for the treatment of wastewater containing phenol provides certain basis.
The Practice and Cogitation of Microbiology Experiment Teaching Reform in Normal college
高师院校微生物实验教学改革的实践与思考

TANG Yun SONG Bo,
,宋波

微生物学通报 , 2010,
Abstract: According to the microbiology experimental teaching system under the new curriculum reform of college,the microbiology experiment guide in normal college has been reformed,and the cultivating students' manipulative ability as the starting point was also put forward.From the reform of the experimental content,teaching method,experimental teaching staff and some other aspects,a set of innovation teaching system on microbiology experiment course to suit the biological science undergraduate in normal college wa...
微型预混腔内流体传质影响因素的数值模拟
张力,,,冉景煜
中国电机工程学报 , 2007,
Abstract: 微型发动机由预混腔和燃烧腔组成,而微型预混腔内流体的流动和传质是影响微型发动机预混催化重整燃烧的重要因素。甲烷和空气分别沿轴向和径向进入微型预混腔,2种气体形成垂直交叉射流,经过微槽道进入环形腔。采用连续介质层流模型二阶离散对三维微型预混腔内流体流动和传质进行了数值模拟,采用相对未混合度表示气体混合的均匀性。结果表明,入口效应和壁面碰撞效应有利于腔体内传质和混合。甲烷和空气的射流动量比、接触面积及停留时间越大,相对未混合度越小。气体在旋流槽中的停留时间较大,而在直流槽中有环形腔壁面碰撞效应增强扰动和混合,基金项目:教育部博士点基金项目(20040611013);中国博士后科学基金项目。因而2种微型预混腔结构都能得到较好的传质效果。混合气体流动为层流,流动阻力较小。
铝合金Ni-Co-P/Si3N4镀层的化学复合镀制备及其硬度
胡佳,方亮,安琼,
重庆大学学报 , 2011, DOI: 10.11835/j.issn.1000-582X.2011.09.020
Abstract: 针对铝合金硬度较低等缺点,采用化学复合镀方法在铝合金表面制备了Ni-Co-P/Si3N4镀层。通过正交试验,研究了铝硅合金基体化学镀Ni-Co-P/Si3N4镀层的工艺参数对镀层硬度的影响。得出镀液pH值、硫酸钴/(硫酸镍+硫酸钴)物质的量比、氮化硅粉末含量、施镀温度对硬度的影响规律。发现:增大pH值以及镀液中氮化硅含量,保持硫酸钴/(硫酸镍+硫酸钴)物质的量比适中、升高施镀温度可以提高镀层硬度。此外,研究了热处理温度对镀层硬度的影响,发现:硬度随着热处理温度升高而增大,当热处理温度超过400℃时,硬度开始下降。
Orlicz-Sobolev空间的端点与严格凸性
The Extreme Points and Rotundity of Orlicz-Sobolev Spaces
 [PDF]

曹法
Pure Mathematics (PM) , 2015, DOI: 10.12677/PM.2015.53018
Abstract:
本文在Orlicz-Sobolev空间上给出了一种模范数,给出了由严格凸N函数生成Orlicz-Sobolev空间严格凸的充要条件。
In this paper we give a modular norm for Orlicz-Sobolev spaces, and obtain a necessary and suffi-cient condition for the Orlicz-Sobolev spaces which is formed by strictly convex N function to be rotund.
带加性噪声的随机非线性Klein-Gordon方程

四川师范大学学报(自然科学版) , 2011,
Abstract: 建立了该方程Cauchy问题的局部适定性,在负参数非线性情形下,根据能量方程和Bore-Cantelli引理证明了解都几乎整体存在.在正参数非线性情形下,根据质量方程证明了对于某些情形时,系统的解爆破,所得结论推广了相关文献的结果.
带无界势的阻尼非线性Schrdinger方程的整体存在性

四川师范大学学报(自然科学版) , 2010,
Abstract: 考虑带无界势的阻尼非线性Schrdinger方程.运用变分方法,导出一个可以刻画该系统整体解的定态方程.结合系统以及该定态方程的特征,构建系统的不变发展流,从而回答了使系统的解整体存在时初值的极小程度
Effect of Metal Ions on Cell Growth and Phenol Degradation of a Thermophilic Strain BF80
金属离子对嗜热菌BF80生长及苯酚降解的影响研究

YANG Feng-Xiao,TANG Yun,
杨峰晓
,

微生物学通报 , 2008,
Abstract: The effect on the growth and phenol degradation of the thermophilic strain BF80 by seventeen different metal ions were studied. The results showed that the metal ions certainly affected the growth and phenol degradation of the strain BF80. At the concentration of 0.01%, contrasting to the comparison, the growth and phenol degradation of the strain BF80 were restrained intensively by the metal ions of Cu2+, Zn2+, Co2+, Ba2+, Hg2+, Ni2+, Ag+, Al3+. The metal ions of Cr2+ restrained the phenol degradation of BF80 strongly while Cr2+ restrained the growth of BF80 faintly. The metal ions of Sn2+, Fe2+, Fe3+, Pn2+ restrained the growth and phenol degradation of the strain BF80 at a certain concentration, and the effect of inhibition increased with the increase of the concentrations of the metal ions. At the low concentration of Mn2+ or Mo2+, the growth and phenol degradation of the strain BF80 were increased, but if the concentration beyond the 0.1%, the growth and phenol degradation of the strain BF80 were inhibited. At the different concentration of Ca2+ or Mg2+, the growth of the strain BF80 were increased and the phenol degradation were accelerated, but the max rate of phenol degradation by BF80 was not influenced obviously. When the medium was added the mixture of metal ions of Mo2+ and Mn2+, the strain BF80 grew better, but the rate of the phenol degradation was lower than that of single Mo2+ and Mn2+.
Effect of Metal Ions on Cell Growth and Phenol Degradation of a Thermophilic Strain BF80
金属离子对嗜热菌BF80生长及苯酚降解的影响研究

YANG Feng-Xiao,TANG Yun,
杨峰晓
,

微生物学报 , 2008,
Abstract: The effect on the growth and phenol degradation of the thermophilic strain BF80 by seventeen different metal ions were studied. The results showed that the metal ions certainly affected the growth and phenol degradation of the strain BF80. At the concentration of 0.01%, contrasting to the comparison, the growth and phenol degradation of the strain BF80 were restrained intensively by the metal ions of Cu2+, Zn2+, Co2+, Ba2+, Hg2+, Ni2+, Ag+, Al3+. The metal ions of Cr2+ restrained the phenol degradation of BF80 strongly while Cr2+ restrained the growth of BF80 faintly. The metal ions of Sn2+, Fe2+, Fe3+, Pn2+ restrained the growth and phenol degradation of the strain BF80 at a certain concentration, and the effect of inhibition increased with the increase of the concentrations of the metal ions. At the low concentration of Mn2+ or Mo2+, the growth and phenol degradation of the strain BF80 were increased, but if the concentration beyond the 0.1%, the growth and phenol degradation of the strain BF80 were inhibited. At the different concentration of Ca2+ or Mg2+, the growth of the strain BF80 were increased and the phenol degradation were accelerated, but the max rate of phenol degradation by BF80 was not influenced obviously. When the medium was added the mixture of metal ions of Mo2+ and Mn2+, the strain BF80 grew better, but the rate of the phenol degradation was lower than that of single Mo2+ and Mn2+.
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