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Search Results: 1 - 10 of 32554 matches for " 和一之 "
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1-磷酸鞘氨醇对2型糖尿病小鼠胰岛β细胞损伤的保护作用
Protective effects of sphingosine-1-phosphate on islet β-cell proliferation and less cell apoptosis in type 2 diabetic mice

,,,,,赵心蕊,施秉银
- , 2018, DOI: 10.7652/jdyxb201806012
Abstract: 摘要:目的 研究1-磷酸鞘氨醇(S1P)对2型糖尿病(T2DM)小鼠胰岛β细胞的促进增殖、抑制凋亡情况和对血糖的影响,以及S1P受体S1PR1-3在T2DM小鼠胰腺中的表达定位,探讨S1P对糖尿病胰岛β细胞损伤的保护作用。方法 雄性C57BL/6J小鼠,高脂饮食联合小剂量链脲佐菌素(STZ)制备T2DM小鼠模型,随机分为S1P高剂量组(S1PH)、S1P低剂量组(S1PL)、糖尿病对照组(DC)及正常对照组(NC),每日予S1PH组和S1PL组S1P溶液100μg/kg及20μg/kg腹腔注射,DC组及NC组予同体积溶剂替代。给药3周后行葡萄糖耐量试验(IPGTT),胰腺组织HE及TUNEL染色,免疫组化检测胰岛中胰岛素、Ki67及S1PR1-3蛋白的表达。结果 S1P给药3周后,S1PL组及S1PH组空腹血糖及IPGTT 2h血糖较DC组轻度下降。胰岛组织HE染色及胰岛素免疫组化染色示S1PL组及S1PH组较DC组胰岛病变减轻。Ki67免疫组化染色示S1PL组及S1PH组胰岛β细胞增殖率较DC组明显升高(P<0.05);TUNEL染色示S1PL组及S1PH组胰岛β细胞凋亡率较DC组明显降低(P<0.05)。胰岛组织免疫组化示S1P受体S1PR1-3均表达于小鼠胰岛β细胞中,在胰腺组织外分泌部表达甚微或不表达。其中S1PL组、S1PH组和DC组S1PR1及S1PR2的表达均较NC组明显增多,各组间S1PR3表达差异无统计学意义。结论 S1P与其受体S1PR1及S1PR2结合能够显著改善T2DM小鼠胰岛β细胞形态,促进小鼠胰岛β细胞增殖、抑制凋亡,提示S1P对T2DM胰岛β细胞损伤有保护作用。S1P/S1PR信号通路在T2DM中发挥着重要作用,有可能成为未来药物治疗的新靶点。
ABSTRACT: Objective To explore the potential protective effects of sphingosine-1-phosphate (S1P) on pancreatic islet β-cell proliferation and less cell apoptosis in type 2 diabetic mice and to detect the localization and expression of S1P receptors in the islets of type 2 diabetic mice. Methods Mice with type 2 diabetes induced by high-fat diet with low-dose streptozotocin were randomly divided into 4 groups: high-dose S1P administration group (S1PH), low-dose S1P administration group (S1PL), diabetic model control group (DC), and normal control group (NC). Exogenous S1P was intraperitoneally injected daily at a dose of 100μg/kg for S1PH group and 20μg/kg for S1PL group for 3 weeks. The potential protective effects of S1P on β-cell proliferation and apoptosis were determined. Intraperitoneal glucose tolerance test (IPGTT) and homeostasis model assessment (HOMA) index were assessed to test the effects of S1P on islet function. Expressions of insulin, Ki67, S1P receptor isoforms (S1PR1, S1PR2 and S1PR3) proteins in pancreatic islets were tested by immunohistochemical staining. Results Three weeks after S1P administration, fasting blood glucose and 2-hour glucose after injection during IPGTT in the two S1P administration groups were decreased slightly compared to DC group. β-cell proliferation rate was higher and the apoptosis rate was lower in S1PH and S1PL groups than in DC group (P<0.05). S1P receptors S1PR1, S1PR2 and S1PR3 were positively stained in the islet cell cytoplasm and membrane. S1PR1 and S1PR2 proteins were significantly expressed in S1PH and S1PL groups and DC diabetic groups compared with NC group (P<0.01), while S1PR3 expression did not significantly differ among these groups. Conclusion Extracellular S1P promotes β-cell proliferation and suppresses cell apoptosis via S1P receptor
麝、獐、麂和鹿间线粒体dna的差异及其系统进化研究
李明,,玉手英利,增田隆,永田纯子,大泰司纪
兽类学报 , 1998,
Abstract: ?用pcr技术和序列测定方法从线粒体dna上得到367bp的细菌色素b基因片段序列。分析得出,麝与獐、麂和鹿的遗传差异在12.53%-14.44%之间,处在科间变化范围之内,在分子水平上进一步表明麝应作为一独立科;獐、麂和鹿间序列的平均差异为10.28%,属于亚科间的差异,与形态研究结果一致。麝与獐、麂和鹿的系统进化中,麝约在600万年前与鹿科分歧,而鹿科的三个亚科是在350-500万年开始分歧;麝与獐、麂和鹿共同组成一单系群,在600万年前具有一共同祖先。
新疆地区图书馆事业调查访问侧记

中国图书馆学报 , 1983,
Abstract:
原麝安徽亚种分类地位的再研究
李明,李元广,,玉手英利,增田隆,永田纯子,大泰纪
科学通报 , 1999,
Abstract: 自分布于安徽大别山的麝标本被定名为原麝安徽亚种以来,就其分类地位的划分产生了众多分岐,不同学者从形态喾生态学方面研究和生态学方面研究得出了多种不同的结论。为了对安徽亚种的分类地位有一明确认可,本研究利用形态特征,头骨形态差异的主成分分析和线粒体DNA细胞色素b的367bp片段序列差异,对该亚种的分类地位进行再探讨。
原麝安徽亚种分类地位的再研究
李明,李元广,,玉手英利,增田隆,永田纯子,大泰纪
科学通报 , 1999,
Abstract: 自分布于安徽大别山的麝标本被定名为原麝安徽亚种以来,就其分类地位的划分产生了众多分歧,不同学者从形态学和生态学方面研究得出了多种不同的结论.为了对安徽亚种的分类地位有一明确认可,本研究利用形态特征、头骨形态差异的主成分分析和线粒体DNA(mtDNA)细胞色素b的367bp片段序列差异,对该亚种的分类地位进行再探讨.经分析得出安徽麝标本与其他麝在外部形态和头骨形态上均存在着明显的差异,而且它们之间的DNA序列差异在6.24%~7.90%,处于鹿类动物的种间差异水平.据此,从形态学和分子水平上认为安徽所产的麝应为一个独立种,即安徽麝(MoschusanhuiensisWang,Huetal).
阿拉善滨藜——滨藜属一新种

植物分类学报 , 1997,
Abstract: Inner Mongolia(内蒙古):Alxa League(阿拉善盟),Alxa Left Banner(阿拉善左旗),Xinhaote(新浩特),roadside,1984-07-22,X.T.Lei(雷喜亭)et al.840535(type,HIMC)。 一年生草本,高约30cm。茎直立,四棱形,无粉,有分枝。下部叶对生,中部和上部
荒漠连蕊芥——内蒙古十字花科一新种

植物分类学报 , 1998,
Abstract: 荒漠连蕊芥新种图1SynstemondeserticolusY.Z.Zhao,sp.nov.TYPE:NeiMonggol(内蒙古),AlxaLeague(阿拉善盟),AlxaRightBanner(阿拉善右旗),Ailibugaisumu(艾力布盖...
内蒙古乌头属二新种

植物分类学报 , 1985,
Abstract:
阿拉善滨藜——滨藜属一新种

中国科学院研究生院学报 , 1997,
Abstract:
荒漠连蕊芥——内蒙古十字花科一新种

中国科学院研究生院学报 , 1998,
Abstract:
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