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Search Results: 1 - 10 of 101290 matches for " 周兵? "
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PD98059对H2O2诱导的人乳腺癌细胞凋亡及相关蛋白的影响
The Effect of the PD98059 on H2O2 Induced Apoptosis and Associated Proteins of Human Breast Cancer Cells
 [PDF]

钱梅, 苏式
Hans Journal of Biomedicine (HJBM) , 2011, DOI: 10.12677/hjbm.2011.12007
Abstract:
目的:探讨细胞外信号调节激酶的上游激酶(MEK)抑制剂PD98059对H2O2诱导的人乳腺癌MCF-7细胞凋亡的影响及其作用机制。方法:H2O2诱导乳腺癌MCF-7细胞凋亡,PD98059干预其细胞凋亡。荧光染料吖啶橙(AO)观察细胞凋亡的形态学变化;流式细胞术法进行凋亡细胞计数;Western blot法检测p53,PCNA,caspase-3、caspase-9和Bcl-2的表达。结果:H2O2刺激细胞4 h、12 h,明显诱导了MCF-7细胞凋亡。PD98059将H2O2诱导的细胞凋亡率分别降低了约4%、14%,与H2O2刺激细胞12 h比较,具有显著的统计学差异(P < 0.05)。H2O2显著提高了p53蛋白的表达,激活了caspase-3与caspase-9蛋白,降低了Bcl-2的表达,而PD98059逆转了H2O2的刺激效应,下调了H2O2导致的p53与PCNA蛋白表达,降低了caspase-3与caspase-9蛋白活性,上调了Bcl-2蛋白表达。结论:MEK抑制剂PD98059抑制了H2O2诱导的MCF-7细胞凋亡,其作用机制与其降低了p53和PCNA蛋白表达和caspase-3与caspase-9蛋白活性以及增加Bcl-2蛋白表达有关。
Objective: to study the effect of MEK inhibitor of PD98059 on apoptosis induced by H2O2 and its mechanisms in human breast cancer MCF-7 cells. Methods: H2O2 inducing apoptosis in MCF-7 cells was set to observe apoptosis and its associated proteins treated in the present or absent of PD98059. The cellular morphology was investigated by AO staining. Apoptosis ratio was analyzed by flow cytometry. The expres-sions of p53, PCNA, caspase-3, caspase-9 and Bcl-2 were observed using Western blot analysis. Results: apoptosis was observed with cells treated H2O2 for 4 or 12 h while the degree of apoptosis was greatly de-creased by 4% or 14% in MCF-7 cells treated with H2O2 and PD98059 together. There was significantly dif-ference between with the treatment of H2O2 and PD98059 in the presence of H2O2 for 12 h (P < 0.05). The increased p53, activated caspase-3 and caspase-9 and decreased Bcl-2 were appeared with H2O2 treatment. However, the levels of p53 and PCNA were downregulated, the extent of caspase-3 and caspase-9 activation was decreased, and the level of Bcl-2 was upregulated in MCF-7 cells treated with PD98059 in the presence of H2O2. Conclusions: MEK inhibitor of PD98059 inhibited apoptosis by downregulating the levels of p53 and PCNA, decreasing the activation of caspase-3 and caspase-9 and upregulating the level of Bcl-2 in MCF-7 cells.
不同高压聚乙烯工艺生产涂覆料性能差异探讨

化工进展 , 2010,
Abstract: 就两种不同工艺产品——茂名石化管式法生产的涂覆牌号2420M和燕山石化釜式法生产的1C7A涂覆牌号在物性、加工性能、相对分子质量分布、结晶行为等差异进行探讨。经对比发现,管式法产品的分子支化程度过低是造成其性能不佳的主要原因,釜式法生产产品具有相对分子质量分布宽、长支链多、挤出收缩小,因此在生产涂覆料方面要优于管式法。
变频恒压供油装置应用浅析

黄金科学技术 , 2005,
Abstract: 黄金矿山恒压供油装置一般用于球磨机、液压圆锥破碎机等设备中。它由内置PID的变频器、开关元件、压力变送装置及标准柜体组成。可取代传统的供油设备。具有压力稳定、维护量小、节能效果显著等特点,是国家重点推广应用的节能新产品。
新疆西昆仑康赛音地区地球化学特征及找矿预测

地质论评 , 2015,
Abstract:
新疆中天山巴伦台地区地球化学特征及找矿预测

地质论评 , 2015,
Abstract:
新疆巴伦台地区铁锰矿床特征与找矿远景

地质论评 , 2015,
Abstract:
中国天然气消费结构的模糊地域聚类
The Fuzzy Regional Clusters of China’s Natural Gas Consumption Structure
 [PDF]

, 尹海彤
Advances in Energy and Power Engineering (AEPE) , 2016, DOI: 10.12677/AEPE.2016.45021
Abstract:
出于提高天然气政策效率、优化天然气消费预测以及改善天然气管网规划等目的,运用基于模糊等价关系闭包的聚类方法对大陆30个省份(不含西藏) 2013年天然气消费结构进行了聚类研究。结果为,这些省份:1) 在0.9的精度水平上,共有22类,其中河北与黑龙江组成1类,山西与湖北组成1类,江西、广东、贵州、云南与陕西组成1类,其余省份各独为1类;2) 在0.8的精度水平上,共有12类,其中北京、天津、内蒙古、上海、福建、河南、海南、重庆、青海及新疆各独为1类,江苏与山东组成一类,其余省份组成1类;3) 在0.7的精度水平上,共有4类,其中重庆独为1类,北京与上海组成1类,福建、河南组成1类;4) 在0.6的精度水平上,共有2类,其中重庆独为1类,其余所有省份组成1类;5) 在0.5的精度水平上,仅有1类。据研究结果,得三个主要结论:1) 地域特征并非中国天然气消费结构差异的唯一因素;2) 重庆、北京、上海、福建以及河南在天然气消费结构方面难以与其它省份聚为1类;湖北与山西以及江西、广东、贵州、云南与陕西在这方面很大程度上又各同属一类;3) 重庆表现出了最明显的独特性。这些现象的深层原因值得进一步研究。
In order to enhance the efficiency of natural gas policy, optimize the forecast of natural gas con-sumption and improve the plan of natural gas pipeline construction, an fuzzy clustering based on the fuzzy equivalence relation’s transitive closure is applied to the natural gas consumption structures of 30 provinces (excluding Tibet) of Mainland China in 2013. The results show that, among those provinces: 1) at the accuracy level of 0.9, there are 22 clusters, with Hebei and Hei-longjiang being one cluster, Shanxi and Hubei being another, Jiangxi, Guangdong, Guizhou and Shannxi being a third and each of the rest along being a single cluster; 2) at the accuracy level of 0.8, there are 12 clusters, with Beijing, Tianjin, Inner Mongolia, Shanghai, Fujian, Henan, Hainan, Chongqing, Qinghai and Xinjiang each being a single cluster, Jiangsu and Shandong being one, and all others being another; 3) at the accuracy level of 0.7, there are 4 clusters, with Chongqing along being a single, Beijing and Shanghai being one, Fujian and Henan being another, and all others be-ing the fourth; 4) at the accuracy level of 0.6, there are 2 clusters, with Chongqing along being a single, and all others being the other; and 5) at the accuracy level of 0.5, there is only 1 cluster. Three points can be concluded from the results. First, no evidence of regional characteristic has been found associated with the structures of natural gas consumption in those provinces. Second, it turns out to be rather difficult for Chongqing, Beijing, Shanghai, Fujian as well as Henan to be classified in one cluster, while Hubei & Shanxi and Jiangxi & Guangdong & Guizhou & Yunnan & Shannxi belong to one cluster rather significantly. And third, Chongqing appears the most unique. The in depth logics of those phenomena are worth further investigation.
政府会计管制的三元逻辑框架
彭韶,
财会月刊 , 2009,
Abstract: 本文通过对政府会计管制进行分类解读,提出并深入探讨了政府会计管制的三元逻辑框架,即会计信息披露管制逻辑、会计信息质量管制逻辑、会计行为管制逻辑,以此来说明政府会计管制的必要性及其内在逻辑性。  【关键词】政府会计管制分类解读三元逻辑框架一、对政府会计管制的分类解读  会计管制是由政府强制实施的外在正式制度,其核心是一系列约束公司会计信息生产、披露行为的规则,包括会计信息披露管制、会计信息质量管制与会计行为管制。会计管制的作用在于提供会计信息生产的质量标准、会计信息披露的数量要求以及产生惩戒威胁,以保证会计信息能准确反映企业的财务状况、经营成果和现金流量状况或经营者的经营业绩。这样一来,就能融合信息经济学和新制度经济学对会计管制的解释。
转座酶的人工改造与修饰
倩倩?,
生物工程学报 , 2014, DOI: 10.13345/j.cjb.130653
Abstract: 转座子是基因组中能发生移动和自主复制的dna片段,随着人们在分子水平上对转座子结构和功能认识的不断深化,许多转座子已被改造为遗传分析的工具应用于基因功能分析、基因转化和基因治疗。然而,天然转座子的转座能力不高是转座子的开发和利用的主要障碍,近几年来,科学家们运用生物信息学和蛋白质工程相结合的方法来构建活性的转座酶,通过氨基酸优化的方法获得自然界不存在的超活性的转座酶,显著地提高了转座子的转座效率,应用于植物转基因和基因标签技术;另一方面,通过蛋白质融合技术构建转座酶嵌合体,改造转座子插入特性,实现其插入位点的人工调控,应用于基因治疗。
基于Spring AOP的有序查找算法的重构
曹大有,
计算机系统应用 , 2007,
Abstract: 数据结构有很多查找方法,大体上可分为有序查找和无序查找.但有序查找在查找效率上要好一些.Spring是J2EE的轻量级架构,它通过反向控制和依赖注射的能力来装配和管理任何种类的应用程序对象(Bean).文中通过Spring AOP框架的前置建议对有序查找进行了重构,使得不关数据是否有序都可以按有序序列进行查找,而且这种重构是无侵入性的.
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