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Search Results: 1 - 10 of 13748 matches for " 劳妙芬 "
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抗T细胞单克隆抗体(211-1)清除骨髓中T细胞的实验研究
卜风荣,,毛秉智,董波,吴祖泽
生物工程学报 , 1986,
Abstract: 本文报告应用抗T淋巴细胞单克隆抗体(211—1)和幼兔补体,体外处理骨髓细胞对GM-cFuc生成率无不良影响,并能清除骨髓中98%以上的T细胞。淋巴细胞转化试验也证明,211—1单克隆抗体可以清除骨髓中T细胞。
重组腺病毒介导人肝细胞生长因子在病理性瘢痕基因治疗研究中的应用
哈小琴,苑宾,李元敏,,吴祖泽
中国科学 生命科学 , 2003,
Abstract: 采用细胞内质粒DNA同源重组方法,将人肝细胞生长因子基因构建于E1,E3区缺失的复制缺陷型5型腺病毒载体上,获得携带人肝细胞生长因子基因(HGF)的重组腺病毒Ad—HGF.在293细胞中扩增后用氯化铯密度梯度离心法扩增制备Ad—HGF和Ad—GFP(携带绿色荧光蛋白报告基因的重组腺病毒),然后,用Ad—GFP和Ad—HGF体外分别转染原代培养的兔耳瘢痕成纤维细胞,观察体外转染效率及表达,并以新西兰兔耳瘢痕为体内模型,观察局部瘢痕组织中一次性注射Ad—HGF后对已形成瘢痕的治疗作用.结果显示:(i)Ad—GFP感染原代培养兔瘢痕成纤维细胞后,3 d时转染效率为(36.8±14.1)%,并持续表达20 d以上.(ii)用ELISA方法检测Ad—HGF感染原代培养兔瘢痕成纤维细胞后HGF的表达,结果感染后3 d表达量约为76 ng/4.0×105细胞.(iii)用兔耳瘢痕模型观察在瘢痕内注射不同剂量(8.6×109,8.6×108,8.6×107,8.6×106pfu)的Ad—HGF,于注射后第32天肉眼可见Ad—HGF治疗组瘢痕较治疗前明显缩小、变薄,甚至与周围皮肤在同一水平.这一效应呈剂量依赖性:大、中剂量组(8.6×109,8.6×108pfu)治疗前后瘢痕肥大指数的减少有统计学意义(P<0.05),尚可见不同程度再生的丛状新生毛,低剂量组(8.6×107,8.6×106pfu)治疗后瘢痕肥大指数有减小但无统计学意义,对照组
重组腺病毒介导人肝细胞生长因子在病理性瘢痕基因治疗研究中的应用
哈小琴,苑宾,李元敏,,吴祖泽
中国科学 生命科学 , 2003,
Abstract: 采用细胞内质粒DNA同源重组方法,将人肝细胞生长因子基因构建于E1,E3区缺失的复制缺陷型5型腺病毒载体上,获得携带人肝细胞生长因子基因(HGF)的重组腺病毒Ad-HGF.在293细胞中扩增后用氯化铯密度梯度离心法扩增制备Ad-HGF和Ad-GFP(携带绿色荧光蛋白报告基因的重组腺病毒),然后,用Ad-GFP和Ad-HGF体外分别转染原代培养的兔耳瘢痕成纤维细胞,观察体外转染效率及表达,并以新西兰兔耳瘢痕为体内模型,观察局部瘢痕组织中一次性注射Ad-HGF后对已形成瘢痕的治疗作用.结果显示:(ⅰ)Ad-GFP感染原代培养兔瘢痕成纤维细胞后,3d时转染效率为(36.8±14.1)%,并持续表达20d以上.(ⅱ)用ELISA方法检测Ad-HGF感染原代培养兔瘢痕成纤维细胞后HGF的表达,结果感染后3d表达量约为76ng/4.0′105细胞.(ⅲ)用兔耳瘢痕模型观察在瘢痕内注射不同剂量(8.6′109,8.6′108,8.6′107,8.6′106pfu)的Ad-HGF,于注射后第32天肉眼可见Ad-HGF治疗组瘢痕较治疗前明显缩小、变薄,甚至与周围皮肤在同一水平.这一效应呈剂量依赖性:大、中剂量组(8.6′109,8.6′108pfu)治疗前后瘢痕肥大指数的减少有统计学意义(P<0.05),尚可见不同程度再生的丛状新生毛,低剂量组(8.6′107,8.6′106pfu)治疗后瘢痕肥大指数有减小但无统计学意义,对照组多数瘢痕大小无明显变化.光学显微镜观察组织切片显示,治疗组真皮中纤维组织明显少于对照组,并可见毛囊及皮脂腺的存在;天狼猩红染色显示大剂量组创口中胶原纤维薄且稀少,而低剂量组和对照组创口中仍有丰富的大而粗的Ⅰ型胶原.(ⅳ)治疗兔体内未检测到抗HGF抗体的存在.(ⅴ)局部应用Ad-HGF所介导的基因只在局部表达,不累及远隔的器官和组织,也未见任何毒副作用.结果提示,以腺病毒介导人肝细胞生长因子基因治疗病理性瘢痕在整形外科中具有一定的应用前景.
抗t细胞单克隆抗体(211-1)清除骨髓中t细胞的实验研究
卜风荣 毛秉智 董波 吴祖泽
生物工程学报 , 1986,
Abstract: 本文报告应用抗t淋巴细胞单克隆抗体(211—1)和幼兔补体,体外处理骨髓细胞对gm-cfuc生成率无不良影响,并能清除骨髓中98%以上的t细胞。淋巴细胞转化试验也证明,211—1单克隆抗体可以清除骨髓中t细胞。
携带肝细胞生长因子基因的重组腺病毒对犬心肌缺血的基因治疗
吴丹莉,张友荣,,袁丽珍,王澜,哈小琴,吴祖泽
科学通报 , 2004,
Abstract: 为了进一步研究重组腺病毒Ad-HGF对心肌缺血的治疗效果及其安全性,在体外实验和大鼠实验的基础上制作了犬心肌缺血的模型,从3个方面评价Ad-HGF对心肌缺血的治疗效果.首先,通过冠状动脉造影观察侧支循环形成的情况,发现治疗组动物的侧支循环比对照组丰富;然后,对心脏切片进行TTC染色,并通过图像分析确定缺血区面积,结果显示治疗组动物的缺血区明显小于对照组;最后,从心肌中回收彩色微球计算局部心肌血流量,结果显示治疗组动物的局部心肌血流量基本恢复到了结扎前的水平.另外,在长期毒性研究中,用中和方法检测了腺病毒抗体产生的情况,发现腺病毒抗体在第4次注射后产生,达到高峰后缓慢下降,于停药12周后消失.总之,Ad-HGF对心肌缺血的治疗是有效的,同时也会诱导机体产生抗体中和体内的腺病毒,这可能是腺病毒载体的清除机制.
腺病毒载体介导肝细胞生长因子基因对大鼠心肌缺血的基因治疗
吴丹莉,张友荣,,邱兆华,哈小琴,吴祖泽
科学通报 , 2002,
Abstract: 为研究腺病毒载体介导肝细胞生长因子对心肌缺血基因治疗的可行性,首先用RT-PCR方法从人胎盘cDNA文库中克隆人肝细胞生长因子(HGF)基因全长编码区cDNA,通过脂质体共转染和细胞内质粒同源重组的方法构建了携带肝细胞生长因子基因的重组腺病毒Ad-HGF.经过在293细胞系内的扩增和氯化铯密度梯度离心获得大量纯化的重组腺病毒.体外实验中用ELISA方法证明肝细胞生长因子能在原代培养的乳大鼠心肌细胞中表达并分泌到培养上清液中.然后用MTT方法测定了其对内皮细胞的刺激增殖作用.活体实验中用绿色荧光蛋白基因作为报告基因检测了腺病毒在大鼠体内的分布和表达持续时间,通过大鼠冠状动脉结扎后制作的心肌缺血动物模型,证明重组腺病毒Ad-HGF对大鼠心肌缺血的治疗是有效的.以上结果说明Ad-HGF的构建是成功的,在体内外都显示了其生物学活性,为基因治疗缺血性心脏病提供了实验依据.
Gene therapy for pathological scar with hepatocyte growth factor mediated by recombinant adenovirus vector
HA Xiaoqin,YUAN Bin,LI Yuanmin,LAO Miaofen,WU Zuze,
哈小琴
,苑宾,李元敏,,吴祖泽

中国科学C辑(英文版) , 2003,
Abstract: A complementary DNA (cDNA) encoding human hepatocyte growth factor was introduced into a replication-defective type 5 adenovirus (lacking E1, E3 domains) vector by homologous recombination of intracellular plasmid DNA, thus a recombinant vector containing HGF (Ad-HGF) was obtained. Ad-HGF and Ad-GFP (adenovirus vector carrying green fluorescence protein gene) were expanded in 293 cells and purified by cesium chloride gradient centrifugation for large-scale preparation, then were infected to the primarily cultured scar fibroblast of rabbit ear to observe the transfer efficiency and expression level of HGF in vitro. To evaluate the effect of Ad-HGF on established scar Ad-HGF solution was injected into excessively formed scar, which bears some clinical and histologic similarities to human hypertrophic scars. The results showed that: (i) the transfer efficiency was 36.8% ±14.1% on day 3 in primarily cultured scar fibroblasts treated with Ad-GFP and lasted more than 20 d; (ii) high-level expression of HGF protein was detected by means of ELISA in supernatant of scar fibroblasts treated with Ad-HGF, the amount of expression was 76 ng/4.0 x 105 cells on day 3; (iii) on day 32 after a single intradermal injection of Ad-HGF at different doses (8.6 x 109 pfu, 8.6 x 108 pfu, 8.6 x 107 pfu, 8.6 x 106 pfu) per scar, most of the scars in the former two dose groups were dramatically flattened, some were even similar to that of the normal skin. The value of Hl (hypertrophie index) showed that there was a therapeutic effect of Ad-HGF on scars at the dose of 109 pfu and 108 pfu. Whereas no therapeutic effects were seen at lower dose (107 pfu and 106 pfu of Ad-HGF) groups. In addition, clusters of hair were observed to different extent on healed wound treated with Ad-HGF. Histopathologic examination revealed that in most healed wounds of Ad-HGF treated group, the dermal layer was thinner, the amount of fibrous tissue was much fewer, and hair follicles growth and sebaceous glands were observed. In Sirius red-stained sections the amount of type I collagen in the Ad-HGF-treated scars was diminished markedly, compared to that in Ad-GFP group, in which a huge amount of type I collagen was still observed; (iv) immune response against HGF was absent. Antibody against HGF was not detectable by ELISA in serum from rabbit treated with Ad-HGF; (v) no local or systemic side-effects and toxicity associated with the gene transfer were found. These results demonstrated the potential use of treating pathologic scar by Ad-HGF, an alterative strategy of gene therapy for scar in clinical practice.
固定床吸附分离C_8芳烃的数学模型
,叶振华
化工学报 , 1986,
Abstract: 以自制和进口Y型合成沸石在室温下液相吸附分离对二甲苯-乙苯二元体系,研究固定床吸附分离C_8芳烃的数学模型.数模中的传质速率方程用总传质系数表征其传质特点,结合相平衡和物料衡算方程,进行数值积分,得到的理论透过曲线与实验曲线相近.为了进一步探索吸附过程动力学和传质机理,对液相控制、固相控制和固液相联合控制三种数学模型进行了初步探索和比较,发现固相控制模型更适合本研究体系.
鹤望兰叶面积数学模拟、花苞包片长度与花茎长度相关性研究

中国生态农业学报 , 2003,
Abstract: 采用几何图形法、叶片长度乘宽度的统计参数法,模拟鹤望兰叶面积结果较好,花苞包片长度与叶面积、花茎长度均有较好的秩相关。
马蹄莲花期调控指标探析

中国生态农业学报 , 2003,
Abstract: 对马蹄莲出花速度、环境温度平行观测及回归分析表明,马蹄莲出花天数与积温呈线性关系,花茎长度与有效积温呈对数关系,出花高度、售花高度与积温三者有较好关联,控制环境温度可有效控制花期。
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