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对3个品种的红花(新疆库车无刺红花、安徽红花、云红3号红花)的核型进行研究。采用酶解去壁低渗火焰干燥法进行染色体制片确定其染色体数目。结果表明：新疆库车无刺红花细胞染色体数目为24条，核型公式为2n = 2x = 24 = 22sm + 2st，属于3B型。核型不对称系数为68.82%。安徽红花的染色体数目为2n = 24。核型公式为2n = 2x = 24 = 18sm + 4m + 2st。属于2B类型，不对称系数为67.57%。云红3号红花染色体数目为2n = 24。核型公式为2n = 2x = 24 = 10m + 10sm + 2st + 2M。属于2B类型，核型不对称系数为60.24%。Karyotypes of 3 different Carthamus tinctorius L. varieties (Xinjiang, Kuche; Anhui; Yunhong 3#- XJ, AH, YH3#) were investigated. The number of chromosomes in Carthamus tinctorius L. was calculated by the method of flame-dying protocol after enzyme digestion of cell wall was used to prepare chromosome. Results showed that the number of chromosomes of XJ was 24. Its Karyotype was formulated as 2n = 2x = 24 = 22sm + 2st which indicated Stebbins’ 3B type. As. K% = 68.82%. The number of chromosomes of AH was 24. Its Karyotype was formulated as 2n = 2x = 24 = 18sm + 4m + 2st which indicated Stebbins’ 2B type. As. K% = 67.57%. The number of chromosomes of YH3# was 24. Its Karyotype was formulated as 2n = 2x = 24 = 10 m + 10sm + 2st + 2m which indicated Stebbins’s 2B type, As. K% = 60.24%.