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Search Results: 1 - 10 of 229879 matches for " 刘博佳 "
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培养基组分对锦带花粉离体萌发的影响
雪莲,,孙?
北方园艺 , 2012,
Abstract: 以锦带新鲜花粉为试材,采用正交设计L16(45),研究了蔗糖浓度、钙离子浓度、硼酸浓度对锦带花粉离体萌发的影响,以期筛选出锦带花粉离体萌发的最适宜培养基成分。结果表明蔗糖浓度和钙离子浓度对花粉萌发和生长影响较大,硼酸的影响不如二者显著。锦带花粉离体萌发最适宜培养基成分为蔗糖25%、硼酸0.016%、钙离子0.0050%。
乙肝病毒生物标志物及其检测方法研究进展
Progress of Hepatitis B Virus Biomarkers and Detection Methods
 [PDF]

张力玲, 隋志伟, 瑛颖, 王晶,, 陈文,
Medical Diagnosis (MD) , 2013, DOI: 10.12677/MD.2013.34013
Abstract:
全世界约有3.5亿人感染乙型肝炎病毒(Hepatitis B virus, HBV),其中中国有约1.2亿人。应用HBV生物标志物检测,对于HBV的诊断、防治至关重要。目前我国针对HBV的检测方法非常多,这些检测方法基于不同的HBV生物标志物和不同的检测原理,其在临床上的应用以及检测效果也有所不同,因此了解HBV生物标志物及其检测方法的最新进展就显得更为重要。本文逐一介绍了HBV的理化特征、结构、生物标志物以及检测方法的研究进展,以供HBV的临床检测参考。
Hepatitis B is one of the most common infectious diseases around the world. About 350 million people are infected with hepatitis B virus totally in the world, and 120 million people are infected in China. It is very important to apply biomarker detection for diagnosis, prevention and treatment of HBV. At present, there are many detection methods of HBV based on different biomarkers and principles, and they have different clinic application and detection effects, so the progress of the HBV biomarkers and detection methods is more and more important. This article introduced the physical and chemical characteristics, structure, biomarkers of HBV and the research progress of analytical methods, in order to provide the reference for the clinical detection of HBV.
增程式电动环卫车动力系统匹配与仿真
解少,玺斌,,魏朗
公路交通科技 , 2014,
Abstract: 进行增程式电动环卫车动力系统的匹配,对驱动电机、增程器和动力电池组等关键部件进行了选型和指标验证。基于Matlab/Simulink搭建了整车正向仿真模型,对增程器在恒功率模式和功率跟随模式两种控制策略下进行了百公里典型城市公交连续工况仿真。结果表明匹配的动力系统能够满足增程式电动环卫车的工况要求;增程器能够在动力电池荷电状态下降到设定值时开启,以延长车辆的续驶里程,并能够使电池组荷电状态维持在一定的区间。从能量消耗来看,基于增程器开关运行的恒功率模式和功率随动模式在我国典型城市公交工况下的平均等效百公里油耗分别为28.70L和29.51L,即恒功率模式的等效百公里燃油消耗比功率跟随模式的等效百公里燃油消耗少0.81L。
干旱胁迫及复水对大花飞燕草幼苗生理特性的影响
张彦妮,,
北方园艺 , 2015, DOI: 10.11937/bfyy.201509018
Abstract: 以大花飞燕草幼苗为试材,研究了干旱胁迫及复水条件对大花飞燕草幼苗膜脂过氧化和保护酶活性、渗透物质等生理特性的影响。结果表明随干旱胁迫时间的延长,细胞膜透性和丙二醛(MDA)含量不断增加,在干旱胁迫的第20天,细胞膜透性和MDA含量均达到最大,此时植物受害严重。叶片中脯氨酸(Pro)含量迅速增加,复水后又开始下降,膜透性得到了一定的恢复。可溶性蛋白质含量不断下降。复水后,植物的可溶性蛋白质含量有所升高。当胁迫20d时,其体内可溶性蛋白质含量最低。随着时间的推移,干旱胁迫下植株体内的过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性均呈先上升后下降的趋势,复水后,POD、SOD活性呈缓慢升高的趋势。第10天时,POD、SOD活性达到最大值,说明此时植物自身有较高的清除活性氧的能力。
绞股蓝皂苷成分的研究进展
史琳,宋东平,潘明,
药物评价研究 , 2011,
Abstract: 绞股蓝是五加科外含有人参皂苷的植物之一,具有滋补保健、抗癌防衰、增强体质和改善脂质代谢等多种功能,近年来一直是国内外学者研究的热点。综述绞股蓝皂苷成分的研究近况,为绞股蓝的进一步研究和开发提供参考,也为临床用药及相关活性成分的确定提供科学依据。
低强度不同频率全身震动治疗对兔膝骨关节炎的影响
王俊,,陈红英,
- , 2017,
Abstract: 目的研究不同频率低强度全身震动治疗(WBV)对于兔膝骨关节炎软骨下骨微结构、软骨降解、骨/软骨转换以及关节功能的影响。方法成年新西兰兔96只,随机分为膝关节前交叉韧带切断术组(ACLT组,16只)和WBV+ACLT组(80只)。各组行左侧ACLT,制备膝骨关节炎模型。术后2个月,ACLT组不行WBV治疗,WBV+ACLT组分别以频率5 Hz、10 Hz、20 Hz、30 Hz、40 Hz进行WBV治疗(每亚组16只),震动幅度2~4 mm,40 min/d,5 d/周,持续治疗8周。治疗8周后采用负重不对称法检测关节疼痛度;尾静脉血液及尿液采集检测骨/软管转换标记物血清软骨寡聚基质蛋白(COMP),骨转换标记物1型/2型胶原羧基末端肽(CTX-Ⅰ、CTX-Ⅱ),运用Micro-CT仪扫描各组模型左侧膝关节,并分析软骨体积和骨微结构;关节软骨采用大体形态学和组织学评分分析。结果治疗8周,与ACLT组相比, 10 Hz (P<0.05)和20 Hz (P<0.01)亚组疼痛改善明显,而40 Hz (P<0.05)亚组则疼痛加重;大体评分和组织学评分,仅40 Hz亚组内侧髁与ACLT组相比评分增加(P<0.05),病变更重;Micro-CT结果显示,关节软骨体积随着频率的改变呈现先增加后减少的变化趋势,以20 Hz亚组与ACLT组相比增加最为明显(P<0.05);骨小梁厚度、骨小梁数量和骨体积分数随频率的增加而增加(P<0.05);CTX-Ⅰ随频率上升表达下降(P<0.05),而COMP和CTX-Ⅱ的表达随频率增加先降低后升高,以20 Hz亚组最低(P<0.05)。结论较低频率(20 Hz)WBV可有效改善关节功能和软骨下骨微结构,增加骨转换,延缓软骨降解,为较优治疗参数。
Objective To determine the effects of low-magnitude whole body vibration (WBV) on the structure and function of subchondral trabecular bones, cartilage degradation, bone/cartilage turnover, and osteoarthritis (OA) joint function. Methods Knee osteoarthritis model was established in 96 rabbits through left anterior cruciate ligament transaction (ACLT). The rabbits were randomly divided into six groups: ACLT control group, WBV+ACLT group (five subgroups, each comprising 16 rabbits receiving 5 Hz, 10 Hz, 20 Hz, 30 Hz and 40 Hz WBV, respectively, with 2-4 mm amplitude for 40 min/d and 5 d/week over a period of 8 weeks). Joint function was tested via weight-bearing asymmetry. The microarchitecture of subchondral trabecular bones was examined using vivo micro-computed tomography (micro-CT). Cartilage samples from knee joints were taken for gross morphology and histology examinations. Serum samples were taken to detect cartilage oligomeric matrix protein (COMP), C-terminal telopeptide of type Ⅰ collagen (CTX)-Ⅰ and urine CTX-Ⅱ. Results Knee joint pain decreased with 10 Hz (P<0.05) and 20 Hz WBV treatment (P<0.05), but increased with 40 Hz treatment (P<0.05). The micro-CT results showed that articular cartilage increased first, peaked at 20 Hz, and then decreased (P<0.05). With increased frequency of WBV, the trabecular number, subchondral bone thickness and bone volume fraction increased, serum CTX-Ⅰ decreased, COMP and CTX-Ⅱ increased, especially at 20 Hz (P<0.05). Conclusion Lower frequency (20 Hz) WBV can improve bone microstructure, increase bone turnover, delay cartilage degeneration and improve limb function of rabbits with OA.
载脂蛋白A5基因多态性与代谢综合征的相关性
Association between apolipoprotein A5 genepolymorphism and metabolic syndrome

,,施秉银,,,高爱
- , 2015, DOI: 10.7652/jdyxb201505017
Abstract: 摘要:目的 探讨载脂蛋白A5(apoA5)基因-1131T>C(rs662799)及1259T>C(rs2266788)单核苷酸多态性与代谢综合征的关系。方法 采用聚合酶联反应及限制性片段长度多态性分析方法测定110例代谢综合征(MS)患者及110例健康对照组的apoA5基因-1131T>C(rs662799)及1259T>C(rs2266788)二位点的基因型,并检测血糖、血脂等指标,比较不同基因型与MS患病风险的关系。结果 -1131CC基因型携带者体重指数(BMI)、甘油三酯(TG)较TC、CC基因型升高,存在统计学差异(P<0.05)。1259CC基因型携带者TG、糖化血红蛋白(HBA??1C)较TC、CC基因型升高,存在统计学差异(P<0.05)。-1131CC基因型携带者发生MS的风险是TT基因型携带者的4.5倍(OR=4.504, 95% CI:1.766~11.490, P=0.002),且都得到统计学支持。结论 apoA5-1131T>C(rs662799)基因多态性与MS的发病可能相关;而apoA5 1259T>C(rs2266788)基因多态性与MS的发病无明显相关性。
ABSTRACT: Objective To investigate the relationship between apolipoprotein A5 (apoA5) gene and metabolic syndrome (MS). Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of apoA5 -1131T>C (rs662799) and 1259T>C (rs2266788) in 110 patients with MS and 110 healthy control subjects. Results The levels of BMI and TG of subjects with apoA5-1131CC genotype were higher than those of subjects with TC and CC genotype (P<0.05). The levels of TG and HBA1C of subjects with apoA5 1259CC genotype were higher than those of subjects with TC and CC genotype (P<0.05). The risk of MS in subjects with apoA5-1131CC genotype was 4.5 times higher than that in subjects with TT genotype (OR=4.504, 95% CI: 1.766-11.490, P=0.002). Conclusion The genetic polymorphism of apoA5 -1131T>C (rs662799) may contribute to an increased risk of MS while that of apoA5 1259T>C(rs2266788) has no obvious relationship with the occurrence of MS
基于字形与语音的音译单元对齐方法
Integrating of Grapheme-Based and Phoneme-Based Transliteration Unit Alignment Method

,徐金安,陈钰枫,张玉洁
- , 2016, DOI: 10.13209/j.0479-8023.2016.001
Abstract: 摘要 为了解决仅采用基于语音或基于字形的音译方法造成的误差过大问题, 以汉英音译为主要研究对象, 运用统计与规则的理论思想, 提出融合基于语音和字形的音译单元对齐方法, 设计了4个实验, 与传统方法进行对比。实验结果显示, 该方法能够很好地提高机器音译的准确性。
Abstract In order to solve the errors caused by only using the pheneme-based method or the grapheme-based method, applying the theory of statistics and rules, this paper proposes a new method for transliteration unit alignment which integrates the two main transliteration methods. Four experiments are designed to compare with the traditional methods. Experimental results show that proposed method outperforms other methods in terms of performance in machine transliteration.
三角形截面碳纤维在复合材料中的分布规律
,武湛君,王荣国,,
科技导报 , 2013, DOI: 10.3981/j.issn.1000-7857.2013.07.004
Abstract: 三角形截面碳纤维在复合材料内部的排布结构像微波暗室一样,能够对入射的电磁波进行多次反射,从而衰减电磁波,使三角形截面碳纤维复合材料具有吸波性能,因此,研究三角形截面碳纤维在复合材料中的排布规律对研究其吸波机理非常重要.采用实验和模拟的方法对三角形截面碳纤维在复合材料中的排布规律进行了分析.首先,通过扫描电子显微镜(SEM)观察了多组三角形截面碳纤维复合材料试样的截面微观形貌,分析了三角形碳纤维在复合材料中的排布角度及均匀性;其次,为进一步研究三角形截面碳纤维复合材料的吸波机理,根据蒙特卡洛原理,通过Matlab软件对三角形截面碳纤维在复合材料中的排布情况进行了模拟.实验及数值模拟结果吻合较好,均表明三角形截面碳纤维在复合材料中的排布是以各种排布角度随机排布的,而三角形碳纤维分布是比较均匀的,没有明显的密布区域和稀疏区域,该模拟方法可以用于模拟不同含量的三角形截面碳纤维在复合材料中的排布规律.
荔枝和龙眼部分珍稀种质的染色体观察
张永福,,王英,潘丽,胡又厘,,赵海燕,成明
园艺学报 , 2010,
Abstract: 以荔枝和龙眼部分种质幼叶为材料,采用等体积饱和对二氯苯和2mmol?l-18–羟基喹啉混合水溶液预处理,卡诺固定液固定,酸解与酶解相结合,改良卡宝品红染色,观察了体细胞染色体数。结果表明,‘裂叶’龙眼为单倍体(2n=x=15),且生长表现良好;用以创建荔枝遗传图谱的作图群体母本‘马贵荔’和父本‘焦核三月红’及用以构建龙眼遗传图谱的两个作图群体母本‘凤梨朵’和‘石硖’,父本‘大乌圆’和‘中秋一号’均为二倍体(2n=2x=30),通过远缘杂交得到的2株荔枝和龙眼的属间杂种a和b也为二倍体,且生长良好;一些性状独特的荔枝,如‘紫娘喜’、‘无核荔’、‘ms-07’、‘sga’、‘ya1’、‘yn4’,以及龙眼‘双季’也均为二倍体。
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