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Search Results: 1 - 10 of 32467 matches for " 傅松滨? "
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Study on the Relationship Between the Resistance to MTX and the Transport Protein Superfamily of ATP-binding Cassette that Induces Multiple Drug Resistance
介导多药耐药的ABC转运蛋白超家族与MTX耐药性的关系研究

ZHANG Chun-Yu,FENG Yuan-Xi,LI Pu,FU Song-Bin,
张春玉
,冯源熙,李璞,

遗传 , 2006,
Abstract: A major problem, especially the multidrug resistance, in chemotherapy was the resistance to the chemotherapeutic agents. ATP-binding cassette transporter superfamily that mediated the efflux of drugs was involved in multidrug resistance. In order to understand the relationship between the resistance to MTX and the transport protein superfamily of ATP-binding cassette, and to investigate the mechanism of resistance to MTX, the study detected the expressions of mdr1, mrp1, mrp2, mrp3, mrp5, mrp6 and abcg2 that encoded the transport proteins by SuperArray analysis and the expressions of MRPland MRP5 proteins by Western blot analysis. The results showed that the multidrug resistance proteins were the chief member of ATP-binding cassette transporter superfamily related to resistance to MTX. And the high expression levels of mrp1 and mrp5 were detected. Moreover, it revealed by SuperArray analysis that expression of mrp5 in MTX-resistant cells was significantly higher than that in normal mouse cells. Besides, corresponding excessive expression of MRP5 protein in MTX-resistant cells was also confirmed by Western blot. So, MRP5 could play important roles in the resistance to MTX and would be a new potential drug target.
The Roles of rab5a Gene in the Mechanism of Tumor Metastasis
rab5a基因在肿瘤转移中的作用研究

SHI Zhong-Cheng,YU Yang,LI Yu,FU Song-Bin,
史忠诚
,于旸,李钰,

遗传 , 2005,
Abstract: To study the role of rab5a gene in the mechanism of tumor metastasis, rab5a gene was stable transfected into AGZY83-a cell, a lung adenocarcinoma cell line with low metastatic potential. Superarray was applied to study the effect of rab5a on the differential expression of genes related to tumor metastasis. Five differentially expressed genes were obtained, including one up-regulated gene s100a4 and 4 down-regulated genes, nm23a, rac1, cst3 and col4a2. These results were confirmed at both the RNA and protein levels. We concluded that rab5a promoted tumor metastasis by affecting multiple genes in several pathways involved in tumor metastasis.
重组阳离子抗肿瘤肽aik的原核表达、纯化及活性测定
范芳芳?,孙慧莹?,徐晖?,刘佳玮?,张海员?,李亦兰?,宁雪莲?,孙悦?,白静?,,周春水?
生物工程学报 , 2015, DOI: 10.13345/j.cjb.150072
Abstract: 利用gateway克隆技术构建重组抗瘤肽aik的原核表达体系,建立表达及纯化重组aik的最优条件,为深入研究和利用aik奠定基础。首先,设计含attb重组位点的引物,通过重叠pcr技术扩增出attb-tev-flag-aik序列,利用bp重组反应将目的序列tev-flag-aik克隆到供体载体pdonr223中,构建入门载体,再通过lr重组反应,将目的序列转移到目的载体pdest15中,构建gst-aik融合蛋白原核表达质粒。随后,在bl21(de3)工程菌中优化诱导融合蛋白表达的条件。以谷胱甘肽磁珠纯化gst-aik融合蛋白,再以rtev酶切除gst,获得flag-aik重组蛋白。最后以mts法检测flag-aik对白血病细胞hl-60的细胞毒性。菌液pcr验证和测序分析表明成功构建了重组抗瘤肽aik的入门质粒和原核表达质粒。在bl21(de3)工程菌中实现了gst-aik融合蛋白的高效可溶性表达。并测得在37℃下以0.1mmol/liptg诱导工程菌(od600=1.0)4h,重组蛋白表达量占菌体总蛋白的30%以上。经gst亲和层析、rtev酶切除gst标签及二次gst亲和层析获得纯度高于95%的flag-aik蛋白。mts法测得所制备的flag-aik蛋白抑瘤活性与化学合成的aik相当。总之,本课题应用gateway克隆系统成功构建了抗瘤肽aik的原核表达质粒,实现了gst-aik融合蛋白的高效可溶性表达,经亲和层析获得了有生物活性的重组aik多肽,为后续深入研究和大规模制备奠定了基础。
Overexpression of Tumor Suppressor Gene P21 in a Pair of Lung Adenocarcinoma Cell Lines,Anip973 and AGZY83-a,with Different Metastasis Potential
高低转移肺腺癌细胞系Anip973和AGZY83-a中P21过表达的研究 Overexpression of Tumor Suppressor Gene P21 in a Pair of Lung Adenocarcinoma Cell Lines,Anip973 and AGZY83-a,with Different Metastasis Potential

WANG Bai-qiu,YAN Cheng-hui,WU Yan,HUANG Cheng-bin,FU Song-bin,LI Pu,
王柏秋
,闫承慧,吴焱,黄承,,李璞

遗传 , 2000,
Abstract: 为探讨肿瘤抑制基因对肺腺癌细胞生长的抑制作用。利用FuGene转染方法将P21基因的表达质粒转入一对分别具高、低转移能力的肺癌细胞系Anip973和AGZY83-a中,对P21蛋白过表达的细胞系进行了细胞生长曲线,克隆形成率,原位末端标记分析和流式细胞仪分析。P21蛋白过表达的一对细胞系细胞生长曲线斜率降低、克隆形式能力下降并出现明显的G1基因阻滞,但未检测到凋亡信号,结果表明P21基因的过表达通
二步层次分析与化工厂厂区环境质量评价

环境科学 , 1990,
Abstract: 本文提出二步层次分析法,即直接-间接给出判断矩阵,将其应用于化工厂厂区环境质量综合评价,并建立了质量评价模型,与层次分析法相比较,该方法具有判断简便、一致性程度高、分析灵敏度高、决策结果的可信度高、受决策者主观臆断影响小、分析监测数据利用率高等特点。经实例分析表明,评价结果与实际状况较符合。
Establishment and Characterization of Human Lung Carcinoma Cell Line HB-99
人肺癌细胞系HB-99的建立及其生物学特征 Establishment and Characterization of Human Lung Carcinoma Cell Line HB-99

黄昀,吴焱,杨焕杰,张临友,,刘权章HUANG Yun,WU Yan,YANG Huan-jie,ZHANG Lin-you,FU Song-bin,LIU Quan-zhang
遗传 , 2001,
Abstract: 利用一例鳞癌手术标本通过原代培养建立了肺癌细胞系命名为HB-99,该细胞系呈单层贴壁生长,从相差显微镜和电镜分析具有细胞的多形性,细胞倍增时间为24小时,克隆形成率40%,染色体改变复杂,众数63-65,将细胞移植到裸鼠体内而生长的肿块具有与原始病人手术标本相似的组织形态,免疫组织化学分析,近100%的细胞表达角蛋白17(CK17),10%的细胞表达波形蛋白(vimentin),根据该细胞系的生物学特征提示HB099是一新建立的肺鳞癌细胞系。
Zymographic Analysis of MMP in Human Lung Adenocarcinoma Cell Lines with Differential Metastatic Potential
不同转移能力的肺腺癌细胞系金属蛋白酶活性分析 Zymographic Analysis of MMP in Human Lung Adenocarcinoma Cell Lines with Differential Metastatic Potential

YAN Cheng-hui,WU Yan,JIN Yan,FU Song-bin,
闫承慧
,吴焱,金焰,YAN Cheng-hui,WU Yan,JIN Yan,FU Song-bin

遗传 , 2000,
Abstract: To investigate the relationship between the MMP activities and metastatic ability in human lung adenocarcinoma cell lines with differential metastatic potential.Nine lung adenocarcinoma cell lines were tested for their MMP activities by zymographic analysis methods.The MMP production capabilities of carcinoma cells rose following the increase of their metastatic potentials.The result suggested that there was some relationship between MMP-9 activties and metastatic potential in human lung adenocarcinomas.
13q14 Aberration is related to the Metastatic Potential of Human NSCLC
13q14断裂重排与非小细胞肺癌转移潜能关系的研究

HUANG Yun,YANG Huan-jie,JIN Yan,LI Hui-Min,FU Song-bin,
黄 昀
,杨焕杰,金 焰,李慧敏,

遗传 , 2005,
Abstract: A large number of numerical and structural aberrations were analyzed in human tumor metastatic cells and 13q14 aberrations were frequently detected in some types of metastatic cancers. The rearrangement of 13q14 was identified previously in two lung adenocarcinoma cell lines with the same origin but different metastatic potential AGZY83-a and Anip973. BRI gene showed different expression levels in the cell lines as revealed by mRNA differential display (mRNA DD) in the two cell lines, and located in 13q14. In order to investigate the relationship between 13q14 abnormalities and tumor metastasis, a painting probe (13q) was used to hybridize three G-banded NSCLC cell lines with different metastatic potential. The major abnormality of 13q differs among different cell lines, including 13q32-33 frequent breakpoint in these three cell lines. But low metastatic potential cell lines PAa, SPC-1-A were not found breakpoint in 13q14, while 95D cell line with high metastatic potential had the common breakpoint 13q14 in two cell clones. The results suggested that the breakage at 13q14 may possibly be related to lung cancer metastasis. The affirmative relationship between 13q14 aberration and NSCLC needs further investigation.
Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines
野生型P53、P16基因协同对肺腺癌细胞系生长抑制作用的研究 Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines

YAN Cheng-hui,WANG Bai-qiu,WU Yan,FU Song-bin,LI Pu,
闫承慧
,王柏秋,吴焱,,李璞

遗传 , 2001,
Abstract: To investigate the suppression effect of tumor suppressor genes in lung adenocarcinoma cell lines,we transferred a pair of lung adenocarcinoma cell lines with different metastasis potential,Anip973(High metastasis potential cell line) and AGZY83-a (Low metastasis potential cell line)and this pair of cell lines transfected with P16 gene:AGZY83-a P16 and Anip973 P16 with wild type P53 gene with adenovirus vector.The suppression effects of P53 gene were evaluated by cell growth curve,MTT,western blotting analysis and TUNEL technique.Overexpression of wild type P53 gene in AGZY83-a,Anip973,Anip973 P16 and AGZY83-a P16 inhibited the growth of these four kinds of lung cancer cells and induced apoptosis of the cells.The suppression effect of P53 gene in Anip973 and Anip973 P16 was higher than AGZY83-a and AGZY83-a P16 while co expression of P53 and P16 in this pair of cell lines inhibited the cells more efficiently comparing with the expression of P53 alone.Wild type P53 gene might act as a candidate gene in lung adenocarcinoma gene therapy while co transfection of P53 and P16 genes was a more effective method.
弹丸滑翔弹道的能量法研究
,王中原
兵工学报 , 2002,
Abstract: ?本文针对弹丸滑翔飞行的运动特性,根据能量守恒原理,采用能量分析法对弹丸在滑翔段的弹道特性进行了研究。分析了弹丸理想滑翔飞行弹道的特点,推导出弹丸实现滑翔飞行的必备条件,以及弹丸滑翔飞行距离的计算公式、计算方法以及近似估算公式,对滑翔飞行初始时刻的选择进行了初步讨论。所得结果对滑翔弹丸的气动力设计和控制系统设计具有一定的参考价值。
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