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Search Results: 1 - 10 of 110155 matches for " 佴文惠 "
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水貂的染色体研究
,陈玉泽,刘瑞清
兽类学报 , 1998,
Abstract: ?采用复制带、c带和硝酸银染色等分带技术研究了水貂的核型和带型。结果表明,2n=30,核型为10(m)+16(sm)+2(a),xx(m)。c-带显示该水貂的一些染色体的结构异染色质比较丰富,从着丝粒区域延伸到两臂上,no.5染色体着丝粒结构异染色质有些弱化;x染色体的结构异染色质较常染色体的丰富。ag-nors有3个,分布在no.8染色体的次缢痕区域和一条no.2染色体长臂接近着丝粒的区域。
A STUDY ON THE CHROMOSOMES OF WHITE-CHEEKED GIBBON(Hylobates leucogenys)
白颊长臂猿染色体的研究

Liu Ruiqing,Nai Wenhui,Chen Yuze,Yu Dinghui,
刘瑞清
,

动物学研究 , 1996,
Abstract: White-cheeked gibbon(H. leucogenys) is distributed in the south of Yunnan, China.The chromosomes of a female and a male were studied by several banding techniques(G-,C -and Ag-staining), and a techniqe for simultaneous exhibiting chromosome replication patterns and late replicating Y chromosome. The diploid chromosome number is 52. There are 22 pairs of metacentrlcs or submetacentrics (No. 1-22); 3 pairs of acrocentrics(Nos. 23-25), and chromosomes Nos. 23-24 with a secondary constriction near the centromere on the short arm.The X chromosome is a large metacentric, and the Y, the smallest acrocentric. Its karyotype for mula is 44(M or SM)+ 6(A), XY(M, A). The centrimeric distribution of heterochromatin has been demonstrated in some chromosomes. In addion, there are 3 kinds of heterochromatin on the chromosomes:(1) ab sent of centromeric heterochromatin,(2) interstitial heterochromatin,(3) terminal heterochromatin.Interstitial heterochromatin has been observed on the blarm of X chromosome. Y chromosome is completely C-band positive. Silver staining revealed 5 chromosomes with NORs in the male specimen.4 Ag-NORs of theth are heated on the secondary constriction of chromosomes 23 and 24 respectivly,and one of them is located on the Y chromosome which has been confirmed by Fluorescence in situ hybridization(FISH) in the same cell. There are 4 chromosomes with Ag-NORs in the female specimen,and they are also located on the secondary constriction of chromosomes 23 and 24. Moreover,the association of Ag-NORs have been observed. Base on the above results,the relationship in gibbons and the way of chromosome evo lution in the genus Hylobates have been discussed.
CHROMOSOME STUDY OF STONE MARTEN(Martes foina intermedia)
石貂的染色体研究

Liu Ruiqing Nie Wenhui Chen Yuze,
刘瑞清
,

动物学研究 , 1995,
Abstract: The karyotype of Martes foina intermedia were studied by C-banding and silver staining. The number of diploid chromosome is 38. Autosomes consist of 7 pairs of metacentrics, 2 pairs of submetacentrics, 9 pairs of subacrocentrics. Chromosome No.18 has a secondary constriction near by centromere on the long arm. X chromosome is a metacentrics and of a size between that of chromosome No.3 and No.4, and the Y, the smallest acrocentric.The centromeric distribution of heterochromatin has been demonstrated in most of chromosomes. In some of chromosome centromericregions are stained slightly C-bands. Moreover, the constitutive heterochromatin of centromere region of chromosome No.1 is apparently disappeared. The whole short arm of chromosome No.9 is found to be a complete heterochromatin one. The secondary constriction region of chromosome No.18 is with heterochromatin. The uncommon distribution of interstitial heterochromatin has observed on the long arm of X chromosome. Y chromosome is completely C-band positive.There is only one pair of Ag-NORs in the silver-stained karyotype, it is located at the secondary constriction of chromosom No.18. These specific distribution of C-bands and relative to karyotype evolution of Martes foina intermedia were discussed.
THE DISTRIBUTION OF SCE AMONG QUAIL EMBRYO CELLS AND AMONG CHROMOSOMES
姐妹染色单体互换在鹌鹑胚胎细胞染色体间及染色体上的分布

Nai Wenhui Zan Ruiguang,
,昝瑞光

动物学研究 , 1991,
Abstract: The distribution of SCE among quail embryo cells coincided with Poisson distribution, while among chromosomes, it was nonrandom. There was a positive correlation (P<0.05) between SCE frequency and the chromosomal relative length, but SCE distribution in each chromosome was not completely in accordance with the relative length of each chromosome. The SCE frequency in each centromeric region was very high and accordance with the relative length of each chromosome. The per cell SCE average value was not significantly influenced by the sex of embryo (P>0.05), but that whether the SCE per Z was effected significantly by sex had not been determined.
两种长臂猿染色体的c带和ag—nors的比较研究
刘瑞清,,陈玉泽,余定会
兽类学报 , 1996,
Abstract: ?本文对长臂猿属(hylobates)有4个亚属,其中白眉长臂猿(h.hoolock)2n=38和白氟长臂猿(h.leucogenys)2n=52的染色体c带和ag—nors进行了比较研究。结果表明,两种长臂猿的c带核型中除多数染色休有着丝点c带外,一些染色体上还出现插入c带和着丝点c带弱化或减少现象;白眉长臂猿有全异染色质臂;白颊长臂猿有较多的端位c带.白眉长臂猿有两个ag-nors,而白颊长臂猿的ag-nors雌体有4个,雄体有5个,y染色体上有nor.本文对长臂猿的核型进化作了讨论。
黑麂和费氏麂四种卫星DNA的克隆特征和染色体定位(英文)
刘 妍,,黄 玲,王金焕,苏伟婷,LIN Chyi Chyang,杨凤堂
动物学研究 , 2008,
Abstract: 近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒-着丝粒融合)是麂属动物核型演化的主要重排方式。尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合。以前的研究发现在赤麂和小麂染色体的大部分假定的串联融合位点处存在着非随机分布的卫星DNA。然而在麂属的其他物种中,这些卫星DNA的组成以及在基因组中的分布情况尚未被研究。本研究从黑麂和费氏麂基因组中成功地克隆了4种卫星DNA(BMC5、BM700、BM1.1k和FM700),并分析了这些卫星克隆的特征以及在小麂、黑麂、贡山麂和费氏麂染色体上的定位情况。结果表明,卫星I和II DNA (BMC5, BM700和FM700)的信号除了分布在这些麂属动物染色体的着丝粒区域外,也间隔地分布在这些物种的染色体臂上。其研究结果为黑麂、费氏麂和贡山麂的染色体核型也是从一个2n=70的共同祖先核型通过一系列的串联融合进化而来的假说提供了直接的证据。
亚洲象的染色体
陈玉泽,刘瑞清,,王金焕,杨梅,何聪郝,包燕芬,
动物学研究 , 1998,
Abstract: 亚洲象的染色体THECHROMOSOMEOFASIANELEPHANT关键词亚洲象,核型,染色体带型KeywordsAsianelephant,Karyotype,Bandingofchromosomes中图分类号Q959845本文1996-08-...
朱鹮细胞系的建立及其生物学特性观察
王金焕,苏榕,苏伟婷,成诚,魏曙光,丁海华,段英,李生斌,
动物学研究 , 2012, DOI: 10.3724/SP.J.1141.2012.06591
Abstract: 濒危级生物朱鹮是朱鹮属的唯一物种.为保藏朱鹮的遗传资源并为开展其相关生物学研究提供方便的实验材料,利用采自雌、雄两只成年朱鹮的皮肤样品,成功建立了两株朱鹮的细胞系.两株细胞系的形态均为典型的成纤维样细胞;免疫荧光染色检测显示细胞强表达平滑肌特异α-actin,表明两株细胞均来源于血管平滑肌组织;生长特性分析显示细胞增殖能力在10代以前较强,在15代以后逐渐减弱,25代以后则生长缓慢,难以生成单层细胞;染色体分析显示,两株细胞系均为正常二倍体,核型为2n=68,ZZ(♂),ZW(♀).该研究首次报道了朱鹮染色体的Ag带、C带和G带,并通过带型分析和荧光原位杂交鉴别了朱鹮的性染色体.该文建系所用的皮肤组织块贴壁种植法,可以应用于其他鸟类,特别是珍稀濒危鸟类细胞系的建立.
中国4种蝙蝠的G-带和C-带
毛秀光,王金焕,苏伟婷,张礼标,赵旭东,韦力,,杨凤堂
动物学杂志 , 2007,
Abstract:
A Study of Chromosome Translocation of Francois'' Monkey by Fluoresence in situ Hybridization (FISH)
应用荧光原位杂交(FISH)技术研究 黑叶猴染色体易位 A Study of Chromosome Translocation of Francois Monkey by Fluoresence in situ Hybridization (FISH)

NAI Wen-Hui,LIU Rui-Qing,CHEN Yu-Ze,WANG Jin-Huan,
,刘瑞清,陈玉泽,王金焕NAI Wen-Hui,LIU Rui-Qing,CHEN Yu-Ze,WANG Jin-Huan

遗传 , 1999,
Abstract: 本文应用染色体荧光原位杂交(FISH)技术,利用人9号和14号染色体特异探针,对深低温冻存和长期传代的黑叶猴细胞株染色体畸变进行了分析。确定在长期冻存和传代过程中,一些黑叶猴细胞在No12和No17染色体之间发生了易位,一条No17染色体发生断裂,断裂点在17q13,断裂片段17q13-17qter易位到一条No12染色体长臂末端,形成一条小的中着丝粒的和一条具较长长臂的衍生染色体即der(17)和der(12)。结果表明,荧光原位杂交技术用人染色体特异探针不仅能检测出人类染色体畸变,也能有效地检测灵长类动物染色体畸变
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