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Search Results: 1 - 10 of 368336 matches for " 乙型肝炎病毒;生物标志物;检测<br>Hepatitis B Virus "
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Progress of Hepatitis B Virus Biomarkers and Detection Methods

张力玲, 隋志伟, 刘瑛颖, 王晶, 傅博强, 陈文, 王佳媛
Medical Diagnosis (MD) , 2013, DOI: 10.12677/MD.2013.34013
全世界约有3.5亿人感染乙型肝炎病毒(Hepatitis B virus, HBV),其中中国有约1.2亿人。应用HBV生物标志物检测,对于HBV的诊断、防治至关重要。目前我国针对HBV的检测方法非常多,这些检测方法基于不同的HBV生物标志物和不同的检测原理,其在临床上的应用以及检测效果也有所不同,因此了解HBV生物标志物及其检测方法的最新进展就显得更为重要。本文逐一介绍了HBV的理化特征、结构、生物标志物以及检测方法的研究进展,以供HBV的临床检测参考。
Hepatitis B is one of the most common infectious diseases around the world. About 350 million people are infected with hepatitis B virus totally in the world, and 120 million people are infected in China. It is very important to apply biomarker detection for diagnosis, prevention and treatment of HBV. At present, there are many detection methods of HBV based on different biomarkers and principles, and they have different clinic application and detection effects, so the progress of the HBV biomarkers and detection methods is more and more important. This article introduced the physical and chemical characteristics, structure, biomarkers of HBV and the research progress of analytical methods, in order to provide the reference for the clinical detection of HBV.
Present Status of Hepatitis B Virus Nucleic Acid Detection Kit in China

隋志伟, 冉令磊, 张玲, 陈文, 王晶, 傅博强, 王佳媛
Medical Diagnosis (MD) , 2014, DOI: 10.12677/MD.2014.42004
Abstract: 我国是乙型肝炎病毒(Hepatitis B virus, HBV)的高发区,应用核酸扩增技术对HBV核酸生物标志物进行定量检测是HBV诊断和治疗监测中的重要方法。然而,目前我国市场上的商业化HBV核酸检测的试剂盒很多,有必要对不同厂商生产的乙肝病毒核酸检测试剂盒进行评价和分析。本文对乙肝病毒核酸检测方法、乙肝病毒核酸检测试剂盒现状和比较分析等方面作一介绍,以供试剂盒使用者和研发者参考。
China is a high incidence of hepatitis B virus; nucleic acid amplification (PCR) quantitative detection of HBV nucleic acid biomarkers is one of the important means in diagnosis and treatment monitoring of hepatitis B virus. However, there are many commercial HBV nucleic acid detection kits in the Chinese market, it is necessary to evaluate and analyze the kit for hepatitis B virus nucleic acid production of different manufacturers. This article introduced HBV nucleic acid detection methods, present status and comparative analysis of HBV nucleic acid detection kits to provide the reference for the users and developers.
年龄和血清HBsAg、HBV DNA预测慢性乙型肝炎肝组织病理状态的研究
- , 2015, DOI: 10.16118/j.1008-0392.2015.01.012
Abstract: 目的构建基于年龄和血清HBsAg、HBV DNA诊断慢性乙型肝炎肝组织不同病理状态的Logistic回归模型,优化血清HBsAg、HBV DNA诊断肝组织不同病理状态的效能。方法 经肝组织活检的慢性乙型肝炎患者472例,其中HBeAg阳性279例,HBeAg阴性193例。血清HBsAg和HBeAg采用Abbott Architect I2000及其配套试剂检测,血清HBV DNA采用实时荧光定量PCR检测。统计分析采用SPSS 13.0软件。结果 HBeAg阳性患者的血清HBsAg和HBV DNA与病理学分级和分期均呈显著负相关(P<0.05);HBeAg阴性患者的血清HBV DNA与病理学分级和分期呈显著正相关(P<0.01)。预测HBeAg阳性和阴性患者不同病理状态的回归模型的预测概率诊断不同病理状态的ROC曲线下面积均显著大于对角参考线下面积(P<0.01)。对HBeAg阳性患者,预测进展期纤维化的回归模型的预测概率和血清HBsAg诊断进展期纤维化的最佳截断值分别为≥0.185和≤3.797 log10IU/ml,其对应的灵敏度、特异度、准确度分别为0.886、0.646、0.706和0.800、0.660、0.695;对HBeAg阴性患者,预测显著纤维化的回归模型的预测概率和血清HBV DNA诊断显著纤维化的最佳截断值分别为≥0.603和≥3.095 log10IU/mL,其对应的灵敏度、特异度、准确度分别为0.636、0.720、0.668和0.669、0.653、0.663。结论 基于年龄和血清HBsAg、HBV DNA构建的Logistic回归模型可提升血清HBsAg、HBV DNA诊断肝组织不同病理状态的效能。
Objective To construct a Logistic regression model based on age and serum HBsAg, HBV DNA for prediction of liver tissue pathological states in patients with chronic hepatitis B. MethodsTotal 472 consecutive chronic hepatitis B patients with pathological diagnoses of liver tissues, including 279 HBeAg-positive and 193 HBeAg-negative patients, were enrolled in present study. Serum HBsAg and HBeAg were determined by Abbott Architect I2000 and auxiliary reagents, serum HBV DNA was determined by real-time fluorescence quantitative PCR. SPSS 13.0 software was used for statistical analyses. ResultsIn HBeAg-positive patients, serum HBsAg and HBV DNA were negatively correlated with pathological grading and staging(P<0.05). In HBeAg-negative patients, serum HBV DNA was positively correlated with the pathological grading and staging(P<0.01). In both HBeAg-positive and HBeAg-negative patients, the area under ROC curve of the regression model for predicting different pathological states was significantly larger than that of the diagonal reference(P<0.01). In HBeAg-positive patients, the optimal cut-offs of predictive probability in the regression model for predicting advanced fibrosis and serum HBsAg for diagnosis of advanced fibrosis were ≥0.185 and ≤3.797 log10IU/ml respectively, and the corresponding sensitivities, specificities, accuracies were 0.886,0.646,0.706 and 0.800,0.660,0.695 respectively. In HBeAg-negative patients, the optimal cut-offs of predictive probability in the regression model for predicting significant fibrosis and serum HBV DNA for diagnosis of significant fibrosis were ≥0.603 and ≥3.095 log10IU/ml respectively, and the corresponding sensitivities, specificities, accuracies were 0.636,0.720,0.668 and 0.669,0.653,0.663, respectively. ConclusionLogistic regression models based on age and serum HBsAg, HBV
绵阳市育龄期妇女乙型肝炎病毒表面抗原与表面抗体的 双阴分布现况
- , 2017,
Abstract: 目的 了解绵阳市育龄期妇女乙型肝炎病毒表面抗原(HBsAg)与表面抗体(HBsAb)的双阴分布现况,为制定育龄期妇女乙肝控制策略提供科学依据。方法 采用多阶段随机抽样方法,抽取绵阳市62 551名15~49 岁妇女作为研究对象,对所有研究对象进行问卷调查并采集其血标本,采用酶联免疫吸附试验(ELISA)对血清中HBsAg和HBsAb进行检测,HBsAg和HBsAb检测结果均为阴性判定为易感。结果 共62 035名育龄期妇女纳入分析,检测出HBV易感者共28 460名,双阴率为45.88%;15~20岁组HBV表面抗原抗体的双阴性最低;汉族HBV表面抗原抗体的双阴性高于其他民族;农村人口HBV表面抗原抗体的双阴性高于城市人口;丧偶/离异HBV表面抗原抗体的双阴性最高,已婚HBV表面抗原抗体的双阴性最低;农民HBV表面抗原抗体的双阴性最高,医务人员HBV表面抗原抗体的双阴性最低;有乙肝家族史育龄期妇女易感性低于无乙肝家族史者;有乙肝接种史者易感性低于无乙肝接种史者,差异均有统计学意义( P<0.005)。结论 绵阳市育龄期妇女HBV表面抗原抗体的双阴性较高,应加强对其HBV 易感性的监测和接种乙肝疫苗,以减少乙肝母婴传播的概率。
Objective To investigate the distribution of women with negative HBsAg and HBsAb at child-bearing age in Mianyang. Methods A total of 62 551 women aged 15-49 yr. were selected randomly using a multistage sampling strategy in Mianyang to participate in a questionnaire survey. Blood samples were collected during the survey. HBsAg and HBsAb were detected by enzyme-linked immunosorbent assay (ELISA). Those who were both HBsAg and HBsAb negative were deemed as susceptible to future infection of HBV. Results Data from 62 035 participants were valid for analysis: 28 460 (45.88%) were both HBsAg and HBsAb negative. Those aged 15-20 yr. had the lowest negative rate. Higher HBsAg and HBsAb negative rates were found in Han (lowest in Tibetan), rural residents, widowed/divorced (lowest in married), peasants (lowest in medical workers), those with a family history of Hepatitis B and without vaccination ( P<0.005). Conclusion A high proportion of women at child-bearing age in Mianyang was found to be HBsAg and HBsAb negative. They should be monitored and vaccinated as a priority population in the prevention of mother-to-children infection of hepatitis B.
Effect of hepatitis B virus X protein on promoting hepatocellular senescence

熊莹晖, 傅永明, 周鹏程, 王晓芳, 黄泽炳, 全俊, 胡兴旺, 范学工
XIONG Yinghui
, FU Yongming, ZHOU Pengcheng, WANG Xiaofang, HUANG Zebing, QUAN Jun, HU Xingwang, FAN Xuegong

- , 2018, DOI: 10.3969/j.issn.1671-9638.2018.07.004
Abstract: 目的探讨乙型肝炎病毒通过乙型肝炎病毒X蛋白(HBx)对肝细胞衰老的影响。方法对HepG2.2.15细胞、HBx沉默HepG2.2.15细胞(HepG2.2.15HBxsi)及HBx过表达HepG2细胞(HepG2HBx)进行β半乳糖苷酶染色,采用Western Blot对肝细胞衰老相关蛋白进行检测;对比分析HBx对肝细胞衰老的影响。结果β半乳糖苷酶染色阳性细胞比例:HepG2.2.15组为(0.480±0.096),HepG2组为(0.016±0.005),两组比较差异有统计学意义(P<0.001);HepG2.2.15HBxsi组为(0.278±0.065),对照组HepG2.2.15HBxnc组为(0.329±0.044),两组比较差异无统计学意义(P=0.092);HepG2HBx组为(0.319±0.033),对照组HepG2HBxcon组为(0.064±0.012),两组比较差异有统计学意义(P<0.001)。Western Blot检测肝细胞衰老蛋白:HepG2.2.15组和HepG2HBx组肝细胞衰老相关蛋白p53及p21表达量均高于其对照组,HepG2.2.15HBxsi组肝细胞衰老相关蛋白p53及p21表达量低于HepG2.2.15HBxnc组。结论乙型肝炎病毒可通过HBx促进肝细胞衰老
- , 2015, DOI: 10.16118/j.1008-0392.2015.04.012
Abstract: 目的评价血清病毒学标记物预测慢性乙型肝炎(CHB)肝组织病理状态的有效性。方法 CHB患者211例,其中HBeAg阳性和阴性患者分别为125例和86例。血清HBsAg、HBeAg、抗-HBc和HBcrAg分别采用化学发光微粒子免疫法和化学发光酶免疫法检测,血清HBV DNA采用实时荧光定量PCR检测。结果 HBeAg阳性患者,血清HBsAg、HBeAg、HBcrAg、HBV DNA与病理学分级和分期均呈显著负相关(P<0.05);血清抗-HBc与病理学分级和分期均呈显著正相关(P<0.05);HBeAg阴性患者,血清HBsAg、抗-HBc与病理学分级和分期无显著相关性(P>0.05);血清HBcrAg、HBV DNA与病理学分级和分期均呈显著正相关(P<0.01)。有序logistic回归分析显示,HBeAg阳性患者,血清HBsAg预测病理学分级和HBsAg、HBeAg预测病理学分期的偏回归系数有统计学意义(P<0.05);HBeAg阴性患者,血清HBcrAg和HBV DNA预测病理学分级和分期的偏回归系数均有统计学意义(P<0.05)。结论 血清HBsAg和血清HBsAg、HBeAg分别有预测HBeAg阳性患者肝组织病理学分级和分期的价值,血清HBcrAg和HBV DNA均有预测HBeAg阴性患者肝组织病理学分级和分期的意义。
Objective To assess the application of serum viral markers in prediction of pathological status of liver tissue in patients with chronic hepatitis B. Methods Two hundred and eleven patients with chronic hepatitis B, including 125 HBeAg-positive and 86 HBeAg-negative patients, were enrolled in present study. Serum HBsAg, HBeAg, anti-HBc and HBcrAg were measured by chemilu-minescence microparticle immunoassay and chemiluminescence enzyme immunoassay, respectively; HBV DNA was assayed by Real Time fluorescent quantitative PCR. ResultsIn HBeAg-positive patients, serum HBsAg, HBeAg, HBcrAg and HBV DNA were significantly correlated negatively with the pathological grading and staging of liver tissue(P<0.05), and serum anti-HBc was significantly correlated positively with the pathological grading and staging of liver tissue(P<0.05). in HBeAg-negative patients, serum HBsAg and anti-HBc were not correlated with the pathological grading and staging of liver tissue(P>0.05), however, serum HBcrAg and HBV DNA were significantly correlated positively with the pathological grading and staging of liver tissue(P<0.01). Ordinal logistic regression analyses showed that in HBeAg-positive patients the partial regression coefficients of serum HBsAg in predicting the pathological grading and serum HBsAg, HBeAg in predicting the pathological staging of liver tissue had statistical significance(P<0.05); in HBeAg-negative patients, the partial regression coefficients of serum HBcrAg and HBV DNA in predicting both the pathological grading and staging of liver tissue had statistical significance(P<0.05). Conclusion Serum HBsAg and HBsAg, HBeAg in HBeAg-positive patients have predictive value to the pathological grading and staging, and serum HBcrAg and HBV DNA in HBeAg-negative patients have predictive significance to the pathological grading and staging of liver tissue
- , 2018,
Abstract: 目的 了解绵阳市农村居民乙肝表面抗原(HBsAg)阳性、HBsAg和乙肝表面抗体(HBsAb)双阴分布的现况,为制定农村乙肝防控策略提供科学依据。方法 采取多阶段随机抽样方法抽取163 797名农村居民作为调查对象,对其进行一对一问卷调查,并采取血标本,采用酶联免疫吸附试验(ELISA)对分离出的血清进行HBsAg和HBsAb检测,分析其表达分布的流行病学特征。结果 调查对象HBsAg总阳性率为6.57%〔95%可信区间(CI):6.45%~6.69%〕,双阴率为40.32%(95%CI:40.36%~40.84%)。男性HBsAg阳性率(7.74%)高于女性(5.73%),双阴率(39.93%)低于女性(40.61%);56~65岁人群HBsAg阳性率(7.36%)最高,86~96岁人群双阴率(47.61%)最高;已婚人群HBsAg阳性率(6.63%)高于未婚人群(5.54%)和离异/丧偶人群(6.11%),离异/丧偶人群的双阴率(43.04%)最高;涪城区HBsAg阳性率(9.23%)高于江油市(5.38%)和安县(5.77%),安县双阴率(55.24%)最高;有乙肝家族史人群的HBsAg阳性率(21.01%)高于无乙肝家族史人群(6.41%),双阴率(30.10%)低于后者(40.60%);有乙肝疫苗接种史人群的HBsAg阳性率(3.78%)和双阴率(37.91%)均低于无乙肝疫苗接种史人群(分别为7.30%和40.92%);上述差异均有统计学意义(P<0.05)。结论 绵阳市农村居民HBsAg阳性率和双阴率均较高,高龄、未接种乙肝疫苗者应重点防控。
Objective To determine the prevalence of positive HBsAg and negative HBsAg populations in rural Mianyang, and provide evidence support for proper immunization strategies. Methods A questionnaire survey was conducted on 163 797 rural residents in Mianyang selected through a multistage random sampling strategy. Serum samples were taken from the participants to detect HBsAg and HBsAb with enzyme-linked immunosorbent assay (ELISA). Results Overall, 6.57% 〔95% confidence interval (CI): 6.45%-6.69%〕 of participants were HBsAg positive. In those with negative HBsAg, 40.32% (95%CI: 40.36%-40.84%) had negative HBsAb. Higher prevalence of positive HBsAg was found in the male participants (7.74%) compared with the females (5.73%). But the male participants with negative HBsAg were less likely to have negative HBsAb (39.93%) than their female counterparts (40.61%). Those aged between 56 and 65 years had the highest prevalence of positive HBsAg (7.36%); whereas, those aged between 86 and 96 years had the highest prevalence of negative HBsAg/HBsAb (47.61%). The participants who were married (6.63%), resided in Fucheng District (9.23%), had a family history of Hepatitis B (21.01%) and were not vaccinated (7.30%) had higher prevalence of positive HBsAg than others. Those who were divorced and widowed (43.04%), resided in An County (55.24%), had no family history of Hepatitis B (40.60%), and were vaccinated (40.92%) had higher prevalence of negative HBsAg/HBsAb than others. These differences were statistically significant (P<0.05). Conclusion A high proportion of ruralresidents in Mianyang are HBsAg positive or HBsAg/HBsAb negative. The older population and those without ]vaccination should be the main target in the prevention and control of hepatitis B.
Expression, purification, and bio-activity analysis of fusion protein HBx-EGFP-TLM

Xiaoyan Shi,Yingying Zhang,Xiaowei Zhou,Jiansheng Lu,Zekun Guo,Peitang Huang,

生物工程学报 , 2011,
Abstract: Hepatitis B virus X protein (HBx) has various functions and plays a crucial role in the development of hepatocellular carcinoma (HCC). However, due to different transfection efficiency levels and experimental approaches, it is difficult to correlate the exact functions of HBx to HBV-associated HCC. In this study, we constructed two prokaryotic expression vectors, pGEX-HBx-EGFP-TLM and pGEX-EGFP-TLM, which expressed HBx-EGFP-TLM and EGFP-TLM fusion proteins respectively. Both vectors contained a coding sequence of TLM transduction motif derived from the PreS2-domain of Hepatitis B Virus surface antigens. In addition, EGFP was expressed as a reporter reflecting the transduction efficiency of TLM. The fusion protein HBx-EGFP-TLM or EGFP-TLM purified from Escherichia coli BL21(DE3) by ?KTATM Purifier system was incubated with AML12 and SMMC-7721 cells. Both Western blotting and laser confocal results indicated that the translocation motif TLM could lead HBx-EGFP and EGFP into the cytoplasm. Dual-Luciferase Reporter Assay revealed that the activity of mEZH2 promoter could be up-regulated by the recombinant HBx. In conclusion, we expressed a cell-permeable HBx, which could provide a new method to study the functions of HBx.
Prokaryotic Expression of Variable Domain of Neutralizing Antibody Against Hepatitis B Virus pre-S1

GUAN Bao_Quan,ZHANG Jun,LUO Wen_Xin,CHEN Min,WU Ting,CHENG Tong,XIA Ning_Shao,

生物工程学报 , 2004,
Abstract: 鼠源单克隆抗体MA18/7是特异识别乙型肝炎病毒 (HBV)pre S1抗原的中和抗体。为表达MA18/7的小分子抗体 ,将MA18/7的重链可变区基因(VH)和轻链可变区(VL)基因分别克隆到原核表达载体pTO-T7进行原核表达。结果VH和VL均以包涵体的形式高效表达 ,包涵体经过变性、复性及金属离子亲和纯化后获得纯度>90%的重组抗体片段。生物传感器、竞争ELISA和间接ELISA测定均显示 ,VH 或VL 均不能结合相应抗原 ,但二者体外混合后可迅速非共价结合形成具有良好活性的可变区抗体Fv,表明MA18/7的抗原结合活性区由重链可变区和轻链可变区共同组成.
- , 2016, DOI: 10.16781/j.0258-879x.2016.12.1464
Abstract: 目的 研究外来务工人员子女乙肝病毒感染及乙肝疫苗免疫现况,为乙肝防治工作提供依据。方法 从上海市浦东新区5个城郊结合社区的幼儿园与中小学中招募1 071名外来务工人员子女开展流行病学问卷调查,采集静脉血进行乙肝血清学指标及HBV DNA浓度检测。结果 乙肝疫苗接种率87.1%(933/1 071),10岁以下学生接种率高于10岁及以上学生(P<0.01),汉族接种率高于少数民族(P<0.001),父母亲文化程度大专及以上学生的接种率高于父母亲文化程度高中及以下的学生(P<0.001)。HBsAg阳性率0.7%(7/1 071),年龄与家庭中有HBV感染亲属为HBsAg阳性的主要影响因素(P<0.05);HBcAb阳性率1.7%(18/1 071),HBcAb阳性率与年龄呈正相关(P<0.01);HBsAb阳性率为33.9%(363/1 071),3~14岁学生HBsAb阳性率随年龄增长而递减,15岁后再次升高(P<0.001),少数民族学生阳性率高于汉族(P<0.001)。HBsAb滴度低水平比例随年龄增大而降低,正常水平与高水平比例随年龄增大而上升(P<0.01)。结论 外来务工人员子女乙肝疫苗接种率低于所在地户籍儿童,HBsAg、HBcAb阳性率较浦东新区2006年阳性率显著下降,建议进一步加强该人群的乙肝疫苗预防接种服务与管理。
Objective To investigate the statuses of hepatitis B virus (HBV) prevalence and hepatitis B vaccination coverage in children of the migrant workers, so as to provide the evidence for HBV control and prevention in Shanghai. Methods An epidemiological survey was conducted with 1 071 children of the migrant workers; the children were from the kindergartens, primary and secondary schools in five urban-rural fringe communities in Pudong New Area of Shanghai. Fasting bloods samples were collected to detect the biomarkers and DNA titer of HBV. Results The hepatitis B vaccination coverage rate was 87.1% (933/1 071); the coverage rate of students under the age of ten was significantly higher than those at 10 years of age or older (P<0.01); the coverage rate in children of the Han nationality was significantly higher than that of the other minorities (P<0.001), and the coverage rate in children whose parents with the college education background and above was higher than that of children whose parents with a lower education background (P<0.001). The positive rate of HBsAg was 0.7% (7/1 071) in this study. We found that age and HBV infected relatives in the family were the main risk factors of HBV infection in children (P<0.05). The positive rate of HBcAb was 1.7% (18/1 071) in the present study, which was positively correlated with the age of children (P<0.01). The positive rate of HBsAb was 33.9% (363/1 071), and the rate decreased with the age increase between 3 to 14 years old and increased with the age increase after 15 years old (P<0.001). The positive rate of HBsAb was significantly higher in other minorities than that in the Han nationality (P<0.001). The proportion of children with low level of HBsAb titer decreased with the increase of age, while the proportion of children with normal and high level of HBsAb increased with the increase of age (P<0.01). Conclusion The hepatitis B vaccination coverage rate in the children of migrant
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