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Search Results: 1 - 10 of 350857 matches for " 乙型肝炎病毒 乙型肝炎表面抗原 乙型肝炎表面抗体 育龄期妇女</br>Hepatitis B virus HBsAg HBsAb Child-bearing women "
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绵阳市育龄期妇女乙型肝炎病毒表面抗原与表面抗体的 双阴分布现况
- , 2017,
Abstract: 目的 了解绵阳市育龄期妇女乙型肝炎病毒表面抗原(HBsAg)与表面抗体(HBsAb)的双阴分布现况,为制定育龄期妇女乙肝控制策略提供科学依据。方法 采用多阶段随机抽样方法,抽取绵阳市62 551名15~49 岁妇女作为研究对象,对所有研究对象进行问卷调查并采集其血标本,采用酶联免疫吸附试验(ELISA)对血清中HBsAg和HBsAb进行检测,HBsAg和HBsAb检测结果均为阴性判定为易感。结果 共62 035名育龄期妇女纳入分析,检测出HBV易感者共28 460名,双阴率为45.88%;15~20岁组HBV表面抗原抗体的双阴性最低;汉族HBV表面抗原抗体的双阴性高于其他民族;农村人口HBV表面抗原抗体的双阴性高于城市人口;丧偶/离异HBV表面抗原抗体的双阴性最高,已婚HBV表面抗原抗体的双阴性最低;农民HBV表面抗原抗体的双阴性最高,医务人员HBV表面抗原抗体的双阴性最低;有乙肝家族史育龄期妇女易感性低于无乙肝家族史者;有乙肝接种史者易感性低于无乙肝接种史者,差异均有统计学意义( P<0.005)。结论 绵阳市育龄期妇女HBV表面抗原抗体的双阴性较高,应加强对其HBV 易感性的监测和接种乙肝疫苗,以减少乙肝母婴传播的概率。
Objective To investigate the distribution of women with negative HBsAg and HBsAb at child-bearing age in Mianyang. Methods A total of 62 551 women aged 15-49 yr. were selected randomly using a multistage sampling strategy in Mianyang to participate in a questionnaire survey. Blood samples were collected during the survey. HBsAg and HBsAb were detected by enzyme-linked immunosorbent assay (ELISA). Those who were both HBsAg and HBsAb negative were deemed as susceptible to future infection of HBV. Results Data from 62 035 participants were valid for analysis: 28 460 (45.88%) were both HBsAg and HBsAb negative. Those aged 15-20 yr. had the lowest negative rate. Higher HBsAg and HBsAb negative rates were found in Han (lowest in Tibetan), rural residents, widowed/divorced (lowest in married), peasants (lowest in medical workers), those with a family history of Hepatitis B and without vaccination ( P<0.005). Conclusion A high proportion of women at child-bearing age in Mianyang was found to be HBsAg and HBsAb negative. They should be monitored and vaccinated as a priority population in the prevention of mother-to-children infection of hepatitis B.
- , 2018,
Abstract: 目的 了解绵阳市农村居民乙肝表面抗原(HBsAg)阳性、HBsAg和乙肝表面抗体(HBsAb)双阴分布的现况,为制定农村乙肝防控策略提供科学依据。方法 采取多阶段随机抽样方法抽取163 797名农村居民作为调查对象,对其进行一对一问卷调查,并采取血标本,采用酶联免疫吸附试验(ELISA)对分离出的血清进行HBsAg和HBsAb检测,分析其表达分布的流行病学特征。结果 调查对象HBsAg总阳性率为6.57%〔95%可信区间(CI):6.45%~6.69%〕,双阴率为40.32%(95%CI:40.36%~40.84%)。男性HBsAg阳性率(7.74%)高于女性(5.73%),双阴率(39.93%)低于女性(40.61%);56~65岁人群HBsAg阳性率(7.36%)最高,86~96岁人群双阴率(47.61%)最高;已婚人群HBsAg阳性率(6.63%)高于未婚人群(5.54%)和离异/丧偶人群(6.11%),离异/丧偶人群的双阴率(43.04%)最高;涪城区HBsAg阳性率(9.23%)高于江油市(5.38%)和安县(5.77%),安县双阴率(55.24%)最高;有乙肝家族史人群的HBsAg阳性率(21.01%)高于无乙肝家族史人群(6.41%),双阴率(30.10%)低于后者(40.60%);有乙肝疫苗接种史人群的HBsAg阳性率(3.78%)和双阴率(37.91%)均低于无乙肝疫苗接种史人群(分别为7.30%和40.92%);上述差异均有统计学意义(P<0.05)。结论 绵阳市农村居民HBsAg阳性率和双阴率均较高,高龄、未接种乙肝疫苗者应重点防控。
Objective To determine the prevalence of positive HBsAg and negative HBsAg populations in rural Mianyang, and provide evidence support for proper immunization strategies. Methods A questionnaire survey was conducted on 163 797 rural residents in Mianyang selected through a multistage random sampling strategy. Serum samples were taken from the participants to detect HBsAg and HBsAb with enzyme-linked immunosorbent assay (ELISA). Results Overall, 6.57% 〔95% confidence interval (CI): 6.45%-6.69%〕 of participants were HBsAg positive. In those with negative HBsAg, 40.32% (95%CI: 40.36%-40.84%) had negative HBsAb. Higher prevalence of positive HBsAg was found in the male participants (7.74%) compared with the females (5.73%). But the male participants with negative HBsAg were less likely to have negative HBsAb (39.93%) than their female counterparts (40.61%). Those aged between 56 and 65 years had the highest prevalence of positive HBsAg (7.36%); whereas, those aged between 86 and 96 years had the highest prevalence of negative HBsAg/HBsAb (47.61%). The participants who were married (6.63%), resided in Fucheng District (9.23%), had a family history of Hepatitis B (21.01%) and were not vaccinated (7.30%) had higher prevalence of positive HBsAg than others. Those who were divorced and widowed (43.04%), resided in An County (55.24%), had no family history of Hepatitis B (40.60%), and were vaccinated (40.92%) had higher prevalence of negative HBsAg/HBsAb than others. These differences were statistically significant (P<0.05). Conclusion A high proportion of ruralresidents in Mianyang are HBsAg positive or HBsAg/HBsAb negative. The older population and those without ]vaccination should be the main target in the prevention and control of hepatitis B.
- , 2015, DOI: 10.16118/j.1008-0392.2015.04.012
Abstract: 目的评价血清病毒学标记物预测慢性乙型肝炎(CHB)肝组织病理状态的有效性。方法 CHB患者211例,其中HBeAg阳性和阴性患者分别为125例和86例。血清HBsAg、HBeAg、抗-HBc和HBcrAg分别采用化学发光微粒子免疫法和化学发光酶免疫法检测,血清HBV DNA采用实时荧光定量PCR检测。结果 HBeAg阳性患者,血清HBsAg、HBeAg、HBcrAg、HBV DNA与病理学分级和分期均呈显著负相关(P<0.05);血清抗-HBc与病理学分级和分期均呈显著正相关(P<0.05);HBeAg阴性患者,血清HBsAg、抗-HBc与病理学分级和分期无显著相关性(P>0.05);血清HBcrAg、HBV DNA与病理学分级和分期均呈显著正相关(P<0.01)。有序logistic回归分析显示,HBeAg阳性患者,血清HBsAg预测病理学分级和HBsAg、HBeAg预测病理学分期的偏回归系数有统计学意义(P<0.05);HBeAg阴性患者,血清HBcrAg和HBV DNA预测病理学分级和分期的偏回归系数均有统计学意义(P<0.05)。结论 血清HBsAg和血清HBsAg、HBeAg分别有预测HBeAg阳性患者肝组织病理学分级和分期的价值,血清HBcrAg和HBV DNA均有预测HBeAg阴性患者肝组织病理学分级和分期的意义。
Objective To assess the application of serum viral markers in prediction of pathological status of liver tissue in patients with chronic hepatitis B. Methods Two hundred and eleven patients with chronic hepatitis B, including 125 HBeAg-positive and 86 HBeAg-negative patients, were enrolled in present study. Serum HBsAg, HBeAg, anti-HBc and HBcrAg were measured by chemilu-minescence microparticle immunoassay and chemiluminescence enzyme immunoassay, respectively; HBV DNA was assayed by Real Time fluorescent quantitative PCR. ResultsIn HBeAg-positive patients, serum HBsAg, HBeAg, HBcrAg and HBV DNA were significantly correlated negatively with the pathological grading and staging of liver tissue(P<0.05), and serum anti-HBc was significantly correlated positively with the pathological grading and staging of liver tissue(P<0.05). in HBeAg-negative patients, serum HBsAg and anti-HBc were not correlated with the pathological grading and staging of liver tissue(P>0.05), however, serum HBcrAg and HBV DNA were significantly correlated positively with the pathological grading and staging of liver tissue(P<0.01). Ordinal logistic regression analyses showed that in HBeAg-positive patients the partial regression coefficients of serum HBsAg in predicting the pathological grading and serum HBsAg, HBeAg in predicting the pathological staging of liver tissue had statistical significance(P<0.05); in HBeAg-negative patients, the partial regression coefficients of serum HBcrAg and HBV DNA in predicting both the pathological grading and staging of liver tissue had statistical significance(P<0.05). Conclusion Serum HBsAg and HBsAg, HBeAg in HBeAg-positive patients have predictive value to the pathological grading and staging, and serum HBcrAg and HBV DNA in HBeAg-negative patients have predictive significance to the pathological grading and staging of liver tissue

GONG Fei JIANG Shu-De LI Wei-Dong CHEN Jun-Ying,

微生物学通报 , 2001,
Abstract: Study the immunological adjuvant function of biodegradable microspheres for DNA immunization. Empty poly (D, L-lactide-co-glycolic acid) microspheres were prepared using the water- inoil-in-water (w-o-w) technique; A plasmid DNA pRc-CMV encoding hepatitis B virus S antigen was constructed; The mixture of the microspheres and the plasmid DNA was prepared by incubation method. The mixture was administered to Balb/c mice by intramuscular injection. Result: The high antibody titer(1:1600) of intramuscular injection of the mixture of microspheres and the plasmid DNA was obtained, similar to that of intramuscular injection of the mixture of AL(OH)3 and hepatitis B virus S antigen; while intramuscular injection the plasmid DNA elicited no serum antibody respones. Conclusion: biodegradable microspheres may be used as an good adjuvant for DNA immunization.
Quantitative detection on different HBsAg levels by chemiluminescence immunoassay and timeresolved immunofluorescence assay

符小玉, 邬飞源, 陈钢, 谢艳玲, 邓国华, 甘绍军, 谭德明
FU Xiaoyu
, WU Feiyuan, CHEN Gang, XIE Yanling, DENG Guohua, GAN Shaojun, TAN Deming

- , 2017, DOI: 10.3969/j.issn.1671-9638.2017.03.018
Abstract: 目的以雅培全自动化学发光免疫分析法(CMIA法)为标准,探讨时间分辨免疫荧光法(TRIFA)检测血清乙型肝炎表面抗原(HBsAg)的准确性和可行性,以便基层医院普遍开展此项目,为抗病毒个体化的策略及疗效的预测提供依据。 方法分别用CMIA法和TRIFA法对157份乙型肝炎病毒(HBV)感染患者的血清进行检测,对于HBsAg滴度超过检测上限的样本,采用稀释液手动稀释后,再进行定量分析。将HBsAg水平分为4组:≤100 IU/mL、101~1 000 IU/mL、1 001~20 000 IU/mL、>20 000 IU/mL,分析两种方法阳性标本定量相关性。结果两种方法的直线回归方程为:Y=2.323X-896.3,相关系数r=0.943,P<0.001。以CMIA法检测值为参考,分为4组进行分析,结果显示在检测低浓度HBsAg样本时,TRIFA数值较CMIA法偏低,而高浓度样本以CMIA法检测值偏高。两种试剂在检测不同浓度的HBsAg均有较好的一致性(均P<0.05),其中以浓度在1 001~20 000 IU/mL时相关性最好。结论两种试剂在HBsAg定量检测上的准确性基本相当,定量相关性以检测值在1 001~20 000 IU/mL之间最佳。TRIFA成本低廉、易操作,更适于基层医院使用,具有广泛的应用前景
年龄和血清HBsAg、HBV DNA预测慢性乙型肝炎肝组织病理状态的研究
- , 2015, DOI: 10.16118/j.1008-0392.2015.01.012
Abstract: 目的构建基于年龄和血清HBsAg、HBV DNA诊断慢性乙型肝炎肝组织不同病理状态的Logistic回归模型,优化血清HBsAg、HBV DNA诊断肝组织不同病理状态的效能。方法 经肝组织活检的慢性乙型肝炎患者472例,其中HBeAg阳性279例,HBeAg阴性193例。血清HBsAg和HBeAg采用Abbott Architect I2000及其配套试剂检测,血清HBV DNA采用实时荧光定量PCR检测。统计分析采用SPSS 13.0软件。结果 HBeAg阳性患者的血清HBsAg和HBV DNA与病理学分级和分期均呈显著负相关(P<0.05);HBeAg阴性患者的血清HBV DNA与病理学分级和分期呈显著正相关(P<0.01)。预测HBeAg阳性和阴性患者不同病理状态的回归模型的预测概率诊断不同病理状态的ROC曲线下面积均显著大于对角参考线下面积(P<0.01)。对HBeAg阳性患者,预测进展期纤维化的回归模型的预测概率和血清HBsAg诊断进展期纤维化的最佳截断值分别为≥0.185和≤3.797 log10IU/ml,其对应的灵敏度、特异度、准确度分别为0.886、0.646、0.706和0.800、0.660、0.695;对HBeAg阴性患者,预测显著纤维化的回归模型的预测概率和血清HBV DNA诊断显著纤维化的最佳截断值分别为≥0.603和≥3.095 log10IU/mL,其对应的灵敏度、特异度、准确度分别为0.636、0.720、0.668和0.669、0.653、0.663。结论 基于年龄和血清HBsAg、HBV DNA构建的Logistic回归模型可提升血清HBsAg、HBV DNA诊断肝组织不同病理状态的效能。
Objective To construct a Logistic regression model based on age and serum HBsAg, HBV DNA for prediction of liver tissue pathological states in patients with chronic hepatitis B. MethodsTotal 472 consecutive chronic hepatitis B patients with pathological diagnoses of liver tissues, including 279 HBeAg-positive and 193 HBeAg-negative patients, were enrolled in present study. Serum HBsAg and HBeAg were determined by Abbott Architect I2000 and auxiliary reagents, serum HBV DNA was determined by real-time fluorescence quantitative PCR. SPSS 13.0 software was used for statistical analyses. ResultsIn HBeAg-positive patients, serum HBsAg and HBV DNA were negatively correlated with pathological grading and staging(P<0.05). In HBeAg-negative patients, serum HBV DNA was positively correlated with the pathological grading and staging(P<0.01). In both HBeAg-positive and HBeAg-negative patients, the area under ROC curve of the regression model for predicting different pathological states was significantly larger than that of the diagonal reference(P<0.01). In HBeAg-positive patients, the optimal cut-offs of predictive probability in the regression model for predicting advanced fibrosis and serum HBsAg for diagnosis of advanced fibrosis were ≥0.185 and ≤3.797 log10IU/ml respectively, and the corresponding sensitivities, specificities, accuracies were 0.886,0.646,0.706 and 0.800,0.660,0.695 respectively. In HBeAg-negative patients, the optimal cut-offs of predictive probability in the regression model for predicting significant fibrosis and serum HBV DNA for diagnosis of significant fibrosis were ≥0.603 and ≥3.095 log10IU/ml respectively, and the corresponding sensitivities, specificities, accuracies were 0.636,0.720,0.668 and 0.669,0.653,0.663, respectively. ConclusionLogistic regression models based on age and serum HBsAg, HBV
四川省绵阳市15~49岁育龄期妇女抗 - HBs流行现状分析
Seroprevalences of hepatitis B surface antibody among 15~49 childbearing-age women in Mianyang, Sichuan Province

- , 2018,
Abstract: 目的 了解四川省绵阳市15~49岁育龄期妇女的抗 - HBs流行现状,为四川社区育龄期妇女乙肝防治管理模式的建立提供科学依据。方法 2014年12月 - 2015年12月,采用多阶段分层整群随机抽样方法,对四川省绵阳市105 606名育龄期妇女进行问卷调查和乙肝血清学检测。结果 绵阳市15~49岁育龄期妇女抗 - HBs阳性60 697人,抗 - HBs阳性率为57.5%;抗 - HBs阳性率随年龄增大而降低,20~24岁组妇女抗 - HBs阳性率最高,为62.3%(7 847/12 589),25~29岁组抗 - HBs阳性率最低,为54.5%(3 722/6 824);医务人员抗 - HBs阳性率最高,为72.5%(980/1 351),农民抗 - HBs阳性率最低,为53.9%(20 432/37 935);不同年龄组、职业、婚姻状况、民族、居住地区及有无乙肝疫苗接种史人群的抗 - HBs阳性率差异均有统计学意义(P<0.05)。 结论 绵阳市15~49岁育龄期妇女抗 - HBs阳性率较低,对HBV感染抵抗力不足,应及时制定与实施育龄期妇女乙肝疫苗加强免疫策略。
Production of Recombinant Humanized anti-HBsAg Fab Antibody by Fermentation
人源性抗HBsAg Fab抗体的发酵生产研究

DENG Ning,XIANG Jun Jian CHEN Wen Yin XIONG Sheng WANG Xun Zhang,SU Kuan Yuan,

生物工程学报 , 2004,
Abstract: In order to produce recombinant human anti-HBsAg Fab antibody in Pichia pastoris, the recombinant yeast was fermented using fed-batch system in a 30 L bioreactor. The fermentation temperature was 30 degrees C, the pH was 5.0 approximately 5.3, and the DO was 20% approximately 30%. The recombinant Fab antibody was purified from crude culture supernatant by ion exchange and analyzed by SDS-PAGE and western blot and ELISA. When the absorbance (OD600) of broth reach 300 at the end of fed-batch phase, the induced phase was initiated. The results showed that recombinant human anti-HBsAg Fab antibody was high-level expressed in recombinant Pichia pastoris using a fed-batch fermentation system. Both chains of the Fab were successfully expressed upon methanol induction. After 192 h of induction, the expression level of recombinant Fab (soluble) reached 412 mg/L. The recombinant Fab antibody was purified effectively by ion-exchange chromatography from the fermentation supernatant to a purity of 95%. And the affinity activities of the purified recombinant Fab antibdy and fermentation supernatant were detected, and both of them showed high affinity activities. The results demonstrated that recombinant human anti-HBsAg Fab antibody could be high level produced by fed-batch fermentations in Pichia pastoris. Which can be efficiently used in industrial production.
Preparation and Application of Monoclonal Antibody Against Hepatitis B Surface Antigen

CHAI Zhi,LUO Xu-guang,XU Jun,ZHANG Yue-hong,XIE Jun,

中国生物工程杂志 , 2008,
Abstract: Objective: Preparation of monoclonal antibody to detect the wild-type HBsAg as well as variant forms. Methods: BALB/c female Mice were immunized with HBsAg isolated from serum of infected patients. mAbs were prepared by hybridoma technology. Identify the purity and characteristics of the selected mAb purified with saturated ammonium sulfate and give preliminary evaluation of the quality and effectiveness of its application, in which polyacrylamide gel electrophoresis and agarose gel electrophoresis and emzyme-linked immunosorbent assay (ELISA) were used. Results: A monoclonal antibody can detect the wild-type HBsAg as well as variant forms was obtained designated as D12. The purity of D12 was high. D12 was highly sensitive and specific to HBsAg and was tested for subtype as IgGl . The recognition sites existed in the nature antigen. The ability to detect the wild-type HBsAg using D12 was better than that of three commercial HBsAg diagnostic kits and the ability to detect the variant HBsAg using D12 was better than that of five commercial HBsAg diagnostic kits. Conclusion: The preparation of mAb D12 is very important to further improve the detection rate of variant hepatitis B virus and enhance HBV prevention.
Effect of routine cleaning and disinfection on eliminating HBV contamination of gastroscopes

SHI Zhimei
,WU Jun,WANG Shuping,LU Rui,WANG Kunke,WANG Shuzhi

- , 2016, DOI: 10.3969/j.issn.1671-9638.2016.05.016
Abstract: 目的了解常规消毒方法对乙型肝炎表面抗原(HBsAg)阳性患者使用过的胃镜乙型肝炎病毒(HBV)污染的清除情况。方法检测2014年12月1—31日于某院行胃镜检查的HBsAg阳性患者使用后的胃镜,根据检查时患者有无黏膜损伤分为黏膜损伤组和黏膜完整组,分别于初洗前、初洗后、消毒后进行3次采样,进行HBsAg、HBVDNA定量检测。结果黏膜损伤组胃镜初洗前HBsAg检测阳性率为93.33%(28/30)、HBVDNA定量检测阳性率为53.33%(16/30),黏膜完整组初洗前HBsAg 定量检测阳性率为80.00%(24/30)、HBVDNA定量检测阳性率为20.00%(6/30);初洗前两组HBVDNA阳性率比较差异有统计学意义(P<0.05);初洗后仅黏膜损伤组HBsAg阳性2例(6.67%),余均为阴性;消毒后两组HBsAg与HBVDNA检测结果均为阴性。结论HBsAg阳性患者使用后的胃镜可以通过规范的常规清洗和消毒达到消毒效果
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