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Search Results: 1 - 10 of 31158 matches for " 丁之铨 "
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杀虫防病基因工程枯草芽孢杆菌的构建
陈中义,张杰,曹景萍,,黄大昉,陈志谊
生物工程学报 , 1999,
Abstract: 分别以枯草芽孢杆菌大肠杆菌穿梭质粒pHB201和pRP22为载体,通过感受态转化方法,将Bt-HD-1杀虫蛋白基因cry1Ac导入了水稻纹枯病生防菌株枯草芽孢杆菌B916。工程菌株质粒酶切电泳分析、Southern印迹分析和杀虫生物活性测定结果证实了cry1Ac基因的导入及其在B916中的有效表达。抑菌测定证明工程菌株保持了原野生型菌株良好的抑菌活性。质粒稳定性分析表明以载体pRP22构建的工程菌株Bs2249具有良好的稳定性,而以载体pBH201构建的工程菌株Bs2014则不稳定。此外,实验还证实Bt基因的导入与表达对B916的生长没有不良影响。
杀虫防病基因工程枯草芽孢杆菌的构建
陈中义 张杰 曹景萍 黄大昉* 陈志谊
生物工程学报 , 1999,
Abstract: 分别以枯草芽孢杆菌大肠杆菌穿梭质粒phb201和prp22为载体,通过感受态转化方法,将bt-hd-1杀虫蛋白基因cry1ac导入了水稻纹枯病生防菌株枯草芽孢杆菌b916。工程菌株质粒酶切电泳分析、southern印迹分析和杀虫生物活性测定结果证实了cry1ac基因的导入及其在b916中的有效表达。抑菌测定证明工程菌株保持了原野生型菌株良好的抑菌活性。质粒稳定性分析表明以载体prp22构建的工程菌株bs2249具有良好的稳定性,而以载体pbh201构建的工程菌株bs2014则不稳定。此外,实验还证实bt基因的导入与表达对b916的生长没有不良影响。
EXPRESSION AND SYNERGISM OF TWO CRY INSECTICIDAL PROTEIN GENES IN PSEUDOMONAS FLUORESCENS
双价杀虫蛋白基因在荧光假单胞菌中的表达及增效

Ding Zhiquan,Zhang Jie,Song Fuping,Huang Dafang,Li Jilun,
,张杰,宋福平,黄大昉,李季伦

微生物学报 , 2000,
Abstract: Several engineered Pseudomonas fluorescens(Pf) strains were constructed mainly based on a Pseudomonas plasmid pJMS6 alpha-lac and two insecticidal crystal protein genes of Bacillus thuringiensis, cry1Ac and cry2Aa, and the host Pf strain, P303, which was with highly antifungal activity to some plant disease fungi and colonizing ability on a wide range of plants. The DNA introduction was confirmed by PCR-RFLP and Southern blot. The 132 kD insecticidal protein was detected in IPP101 and IPP202 by SDS-PAGE and rhombic insecticidal protein crystals of them were observed through electron microscope, also indicating that cry1Ac gene was highly expressed. The results of insecticidal bioassay indicated that IPP101 was more toxic than IPP201, and IPP202 was the most toxic among the 3 strains. LC50 to the neonates of cotton boll worm(Helicoverpa armigera) were 0.02604, 0.00812 and 0.00186 mL/g feed, consecutively. In IPP202, two gene products showed significant synergism, with the co-toxicity coefficient of 332.8.
SOME BIOLOGICAL CHARACTERISTICS OF GENETICALLY ENGINEERED INSECTICIDAL PSEUDOMONAS FLUORESCENS
杀虫遗传工程荧光假单胞菌IPP202部分生物学特性

Z Ding,J Zhang,Z Chen,D Huang,J Li,
,张杰,陈中义,黄大昉,李季伦

微生物学报 , 2001,
Abstract: Plasmid stability, Antifungal activity, plant-colonizing ability, UV resistance and inseticidal activity in field were analysed for the engineered Pseudomonas fluorescens (Pf) strain IPP202. The results indicated that the recombinant plasmid in IPP202 was very stable after successive diluting culturing and after continuous culturing, There was no significant change in the properties beneficial to plants, such as antifungal activity and plant-colonizing ability as compared with the original strain P303. IPP202 was much more resistant to UV than Bt strain HD73. The control effect in field against cotton boll worm in field was close to that of a locally used Bt-chemical mixture in normal applied concentration. All the data indicated that the engineered Pf strain was a one with prosperous future after further study.
建立低阶模型的POD方法
,陶文
工程热物理学报 , 2009,
Abstract: 将分析湍流拟序结构中的最佳正交分解技术运用到流动和传热问题中,并使用最佳正交分解技术建立了物理问题的低阶模型。通过对样本矩阵实施最佳正交分解得到一组特征函数。将控制方程采用Galerkin投影的方法投影到基函数所代表的空间上,从而得到原物理问题的低阶模型。计算结果表明,通过POD方法得到的低阶模型可以非常准确,并且快速地得到问题的解。?
管壁导热对脉管内自然对流换热影响的研究
文静,何雅玲,陶文
工程热物理学报 , 2003,
Abstract: 本文计算了不同脉管倾角和管壁材料下脉管管壁导热对自然对流换热的影响。发现脉管壁面导热对换热的影响不仅体现在增加了壁面的纯导热部分,更主要的上强化了脉管内的自然对流;壁面和内部气体的温度差异沿脉符轴向的变化是管壁导热强化自然对流的主要原因。
基于脉管制冷机的二维可压缩流动数值计算程序的开发与应用
文静,何雅玲,陶文
工程热物理学报 , 2004,
Abstract: 本文在SIMPLEC计算程序的基础上,开发了适用于各种流动Mach的计算程序,既能用于计算不可压缩流体,又可以计算可压缩流动。利用此程序计算了一些典型算例,和有关文献结果符合较好。最后利用可压缩程序计算了小孔型脉管制冷机当小孔处于最佳开度时小孔处的气流分布。
基于POD方法的湍流大尺度信息提取与分析
阳祥,,李增耀,陶文
工程热物理学报 , 2010,
Abstract: 本文用正交分解(POD)方法对径向旋转方通道内直接数值模拟得到的湍流数据进行处理得到了湍流大尺度结构和POD模态的能量分布。对于不同的工况,文中均采用了200个具有相同时间间隔的湍流瞬时主流速度场作为样本。为研究浮升力对湍流大尺度结构的影响及POD模态的能量分布,对四个不同格拉晓芙数Gr,下的主流速度场进行了POD处理和分析。结果表明:湍流大尺度结构主要集中在通道的非稳定壁面(可=0)附近和侧壁面附近,随着格拉晓芙数的增加湍动能分布于更多的模态中。
基本型脉管制冷机的全三维数值模拟计算
文静,何雅玲,陶文
工程热物理学报 , 2006,
Abstract: 建立了基本型脉管制冷机的三维物理和数学模型,在可压缩流动程序的基础上,开发了基本型脉管制冷机的三维非稳态可压缩交变流动与传热耦合计算的数值模拟计算程序。考察了截面平均参数在一个周期内随时间的变化情况,详细分析了脉管内的流场和温度场以及其他各主要参数的变化,对脉管制冷机的工作过程和制冷机理有了进一步的了解和认识,为脉管制冷机的结构改进和实用化奠定了一定的基础。
激光二极管侧面泵浦高功率266nm紫外激光器
李斌,姚建,,张帆,王鹏
强激光与粒子束 , 2011,
Abstract: ?报道了一种半导体激光列阵侧面泵浦nd:yag四倍频266nm全固态紫外激光器,采用z型腔结构,ⅰ类临界相位匹配lbo和bbo晶体分别作为二倍频晶体和四倍频晶体。在调制频率为5khz时,最终获得了2.1w的266nm紫外激光输出,单脉冲能量420μj,绿光到紫外激光的转换率为13.13%,在相同的泵浦功率下利用v型腔结构仅获得305mw的266nm紫外激光输出。
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