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Search Results: 1 - 10 of 4724 matches for "  Hitoshi Takahashi "
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Rupture of the Anterolateral Papillary Muscle Caused by a High Lateral Branch Occlusion  [PDF]
Kayo Sugiyama, Masaki Kano, Satoshi Takahashi, Nobusato Koizumi, Hitoshi Ogino
Open Journal of Thoracic Surgery (OJTS) , 2018, DOI: 10.4236/ojts.2018.81002
Abstract: Although necrosis of the left ventricular papillary muscles during acute myocardial infarction is common, rupture of the anterolateral papillary muscle due to occlusion of high lateral branch is rare. We present a rare case of anterolateral papillary muscle rupture caused by occlusion of a high lateral branch of the left coronary artery. Although the patient was in cardiogenic shock on admission, she was successfully treated by emergency mitral valve replacement after percutaneous catheter intervention and intra-aortic balloon pumping support. This case implies that the anterolateral papillary muscle can have a single blood supply and that it can be ruptured by an occlusion of a high lateral branch. Prompt diagnosis, immediate mechanical stabilization and aggressive surgical treatment are essential to save this group of patients.
Oligonucleotide Microarray Analysis of Age-Related Gene Expression Profiles in Miniature Pigs
Junko Takahashi,Masaki Misawa,Hitoshi Iwahashi
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019761
Abstract: Miniature pigs are useful model animals for humans because they have similar anatomy and digestive physiology to humans and are easy to breed and handle. In this study, whole blood microarray analyses were conducted to evaluate variations of correlation among individuals and ages using specific pathogen-free (SPF) Clawn miniature pigs. Whole blood RNA is easy to handle compared to isolated white blood cell RNA and can be used for health and disease monitoring and animal control. In addition, whole blood is a heterogeneous mixture of subpopulation cells. Once a great change occurs in composition and expressing condition of subpopulations, their associated change will be reflected on whole blood RNA. From 12 to 30 weeks of age, fractions of lymphocytes, monocytes, neutrophils, eosinophils, and basophils in white blood cells showed insignificant differences with age as a result of ANOVA analysis. This study attempted to identify characteristics of age-related gene expression by taking into account the change in the number of expressed genes by age and similarities of gene expression intensity between individuals. As a result, the number of expressed genes was less in fetal stage and infancy period but increased with age, reaching a steady state of gene expression after 20 weeks of age. Variation in gene expression intensity within the same age was great in fetal stage and infancy period, but converged with age. The variation between 20 and 30 weeks of age was comparable to that among 30 weeks individuals. These results indicate that uniformity of laboratory animals is expected for miniature pigs after 20 weeks of age. Furthermore, a possibility was shown that whole blood RNA analysis is applicable to evaluation of physiological state.
Transcriptome Analysis of Porphyrin-Accumulated and X-Ray-Irradiated Cell Cultures under Limited Proliferation and Non-Lethal Conditions
Junko Takahashi,Masaki Misawa,Hitoshi Iwahashi
Microarrays , 2015, DOI: 10.3390/microarrays4010025
Abstract: 5-Aminolevulinic acid (ALA) is a precursor of the photosensitizer used in photodynamic therapy. It accumulates in tumor cells and subsequently metabolizes to protoporphyrin IX (PpIX), which generates singlet oxygen after light irradiation. PpIX enhances the generation of reactive oxygen species following physicochemical interactions with X-rays. ALA-based treatment using fractionated doses of irradiation suppressed tumor growth in a mouse melanoma model. To study the transcriptomic effects of PpIX, microarray analyses were conducted using HeLa cells with limited proliferation capacity. Based on the p-values ( p < 0.01), we selected genes showing altered expression in each treatment group with reference to the non-treatment (NT) group. We detected 290, 196 and 28 upregulated genes, as well as 203, 146 and 36 downregulated genes after a 6 h-long PpIX treatment (1 μg/mL) prior to 3 Gy X-ray irradiation (PpIX-XT), 3 Gy X-ray irradiation alone (XT) and PpIX treatment alone (PpIXT), respectively. Functional analysis revealed that a majority of the regulated genes in the XT and PpIX-XT groups were related to cell-cycle arrest. The XT and PpIX-XT groups differed in the quantity, but not in the quality of their gene expression. The combined effect of PpIX and X-ray irradiation sensitized HeLa cells to X-ray treatment.
Pituitary intratumoral hemorrhage during radiation therapy following partial removal of giant pituitary adenoma: A case report  [PDF]
Junyang Liu, Yuichiro Yoneoka, Kensuke Tanaka, Hiraku Satou, Eisuke Abe, Naoto Watanabe, Yukihiko Fuji, Hitoshi Takahashi, Hidefumi Aoyama
Case Reports in Clinical Medicine (CRCM) , 2014, DOI: 10.4236/crcm.2014.31010
Abstract:


We report a rare case of intratumoral hemorrhage during postoperative radiotherapy for pituitary adenoma. A 57-year-old Asian male, complaining of long-standing eye strain, underwent a medical checkup of the brain. Magnetic resonance imaging showed a multicystic giant pituitary adenoma. The patient underwent an endoscopic endonasal transsphenoidal partial removal of the adenoma to provide optic pathway decompression and got relief from the visual symptoms. Just before completion of the postoperative radiotherapy for residual adenoma, the patient developed right hemiparesis, mild motor aphasia, and right oculomotor palsy. A cranial CT scan showed intratumoral hemorrhage into the intratumoral cyst. The patient therefore had to undergo three additional craniotomies for evacuation of cyst contents over the next 8 months. The follow-up MRI at 11 months after the initial hemorrhage showed that the new oozing of blood in the intratumoral cyst was still appearing. Intratumoral hemorrhage is a rare, albeit life-threatening, complication of pituitary adenoma. We reviewed relevant literature and suggested that the cystic component in pituitary adenoma could be a key pathogenesis of this rare complication. In conclusion, we suggest that it may be necessary to realize that cases which have cystic giant pituitary adenoma may cause hemorrhage by chance with the foreseeability.


Lipoprotein Lipase as a Candidate Target for Cancer Prevention/Therapy
Shinji Takasu,Michihiro Mutoh,Mami Takahashi,Hitoshi Nakagama
Biochemistry Research International , 2012, DOI: 10.1155/2012/398697
Abstract: Epidemiological studies have shown that serum triglyceride (TG) levels are linked with risk of development of cancer, including colorectal and pancreatic cancers, and their precancerous lesions. Thus, it is assumed that serum TG plays an important role in carcinogenesis, and the key enzyme lipoprotein lipase (LPL), which catalyzes the hydrolysis of plasma TG, may therefore be involved. Dysregulation of LPL has been reported to contribute to many human diseases, such as atherosclerosis, chylomicronaemia, obesity, and type 2 diabetes. Recently, it has been reported that LPL gene deficiency, such as due to chromosome 8p22 loss, LPL gene polymorphism, and epigenetic changes in its promoter region gene, increases cancer risk, especially in the prostate. In animal experiments, high serum TG levels seem to promote sporadic/carcinogen-induced genesis of colorectal and pancreatic cancers. Interestingly, tumor suppressive effects of LPL inducers, such as PPAR ligands, NO-1886, and indomethacin, have been demonstrated in animal models. Moreover, recent evidence that LPL plays important roles in inflammation and obesity implies that it is an appropriate general target for chemopreventive and chemotherapeutic agents.
Laminar Mixing in Stirred Tank Agitated by an Impeller Inclined
Koji Takahashi,Yoshiharu Sugo,Yasuyuki Takahata,Hitoshi Sekine,Masayuki Nakamura
International Journal of Chemical Engineering , 2012, DOI: 10.1155/2012/858329
Abstract: The mixing performance in a vessel agitated by an impeller that inclined itself, which is considered one of the typical ways to promote mixing performance by the spatial chaotic mixing, has been investigated experimentally and numerically. The mixing time was measured by the decolorization method and it was found that the inclined impeller could reduce mixing time compared to that obtained by the vertically located impeller in laminar flow region. The effect of eccentric position of inclined impeller on mixing time was also studied and a significant reduction of mixing time was observed. To confirm the experimental results, the velocity profiles were calculated numerically and two novel numerical simulation methods were proposed. 1. Introduction In recent years, many theoretical and experimental studies have been carried out for laminar chaotic mixing and have provided much beneficial information on how laminar mixing can be enhanced. However, it has also been recognized that the mixing in periodic flows is not necessarily complete, because such systems often display coexisting chaotic and nonchaotic regions. Fluids can neither penetrate nor leave these islands of unmixed fluids by regular motion. Therefore, the isolated mixing regions may become barriers to mixing. In the pioneering studies in this field, Lamberto et al. [1] first attempted to solve this problem using time-dependent rotational velocity to enhance the mixing in a stirred tank equipped with ordinary small impellers. After that, several experimental studies have been also undertaken [2, 3] to demonstrate that the mixing performance can be improved markedly by increasing the chaotic degree in temporal terms. While the chaotic degree can be increased effectively by temporal terms, the approach is restricted to practical applications because of the restriction of the motor and the speed reducer machine to drive the impeller. In practice, the temporal approach has rarely been employed in the mixing industry except for washing machines. In contrast, the chaotic degree can also be increased by spatial terms, for example, by reducing the circumferential symmetry and shifting the complexity in normal mixing equipment. While this spatial method does not improve the mixing performance as impressively as the temporal method, it places less demanding requirements on the machinery. Consequently, this approach has been widely used in industry. The special measures employed in an agitated vessel related to spatial chaotic mixing include baffles, off-center impeller mounting, and uncircumferentially
Experimental Animal Models of Pancreatic Carcinogenesis for Prevention Studies and Their Relevance to Human Disease
Mami Takahashi,Mika Hori,Michihiro Mutoh,Keiji Wakabayashi,Hitoshi Nakagama
Cancers , 2011, DOI: 10.3390/cancers3010582
Abstract: Pancreatic cancer is difficult to cure, so its prevention is very important. For this purpose, animal model studies are necessary to develop effective methods. Injection of N-nitrosobis(2-oxopropyl)amine (BOP) into Syrian golden hamsters is known to induce pancreatic ductal adenocarcinomas, the histology of which is similar to human tumors. Moreover, K- ras activation by point mutations and p16 inactivation by aberrant methylation of 5’ CpG islands or by homozygous deletions have been frequently observed in common in both the hamster and humans. Thus, this chemical carcinogenesis model has an advantage of histopathological and genetic similarity to human pancreatic cancer, and it is useful to study promotive and suppressive factors. Syrian golden hamsters are in a hyperlipidemic state even under normal dietary conditions, and a ligand of peroxizome proliferator-activated receptor gamma was found to improve the hyperlipidemia and suppress pancreatic carcinogenesis. Chronic inflammation is a known important risk factor, and selective inhibitors of inducible nitric oxide synthase and cyclooxygenase-2 also have protective effects against pancreatic cancer development. Anti-inflammatory and anti-hyperlipidemic agents can thus be considered candidate chemopreventive agents deserving more attention.
Influence of serotonergic/noradrenergic gene polymorphisms on nausea and sweating induced by milnacipran in the treatment of depression
Hisashi Higuchi, Hitoshi Takahashi, Mitsuhiro Kamata, Keizo Yoshida
Neuropsychiatric Disease and Treatment , 2009, DOI: http://dx.doi.org/10.2147/NDT.S4369
Abstract: fluence of serotonergic/noradrenergic gene polymorphisms on nausea and sweating induced by milnacipran in the treatment of depression Original Research (4508) Total Article Views Authors: Hisashi Higuchi, Hitoshi Takahashi, Mitsuhiro Kamata, Keizo Yoshida Published Date July 2009 Volume 2009:5 Pages 393 - 398 DOI: http://dx.doi.org/10.2147/NDT.S4369 Hisashi Higuchi1, Hitoshi Takahashi2, Mitsuhiro Kamata3, Keizo Yoshida4 1Department of Psychiatry, St. Marianna University, School of Medicine, Kanagawa, Japan; 2Department of Psychiatry, Tokyo Women’s Medical University, School of Medicine, Tokyo, Japan; 3Department of Psychiatry, Yuri-Kumiai General Hospital, Yuri-Honjo, Akita, Japan; 4Department of Psychiatry, Nagoya University School of Medicine, Aichi, Japan Abstract: The present study was conducted to find out the predictors of side effects such as nausea and excessive sweating induced by milnacipran, a serotonin/norepinephrine reuptake inhibitor. Both clinical characteristics prior to the treatment and gene polymorphisms such as serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR), a variable number of tandem repeats in the second intron of the 5-HTT gene (5-HTTVNTR), 5-HT2A receptor gene (5-HT2A G-1438A), a TPH gene polymorphism in intron 7 (TPH A218C), norepinephrine transporter (NET) gene polymorphism in the promoter region (NET T-182C) and in the exon 9 (NET G1287A), a variable number of tandem repeats in the promoter region of monoamine oxidase A, were items to be assessed in this study. Ninety-six patients with major depressive disorder were treated with milnacipran. Side effects were assessed at 1, 2, 4, and 6 weeks of treatment with Udvalg for Kliniske Undersogelser side effects scale. The results showed that no gene polymorphisms included in this study affected the susceptibility of nausea and excessive sweating induced by milnacipran. Patients with older age are more likely to develop excessive sweating than others. The major limitation of this study is a small sample size. Further studies with larger populations and more kinds of gene polymorphisms should be needed to see if specific gene polymorphisms determine the susceptibility of side effects induced by milnacipran.
Influence of serotonergic/noradrenergic gene polymorphisms on nausea and sweating induced by milnacipran in the treatment of depression
Hisashi Higuchi, Hitoshi Takahashi,Mitsuhiro Kamata,Keizo Yoshida
Neuropsychiatric Disease and Treatment , 2009,
Abstract: Hisashi Higuchi1, Hitoshi Takahashi2, Mitsuhiro Kamata3, Keizo Yoshida41Department of Psychiatry, St. Marianna University, School of Medicine, Kanagawa, Japan; 2Department of Psychiatry, Tokyo Women’s Medical University, School of Medicine, Tokyo, Japan; 3Department of Psychiatry, Yuri-Kumiai General Hospital, Yuri-Honjo, Akita, Japan; 4Department of Psychiatry, Nagoya University School of Medicine, Aichi, JapanAbstract: The present study was conducted to find out the predictors of side effects such as nausea and excessive sweating induced by milnacipran, a serotonin/norepinephrine reuptake inhibitor. Both clinical characteristics prior to the treatment and gene polymorphisms such as serotonin transporter (5-HTT) gene-linked polymorphic region (5-HTTLPR), a variable number of tandem repeats in the second intron of the 5-HTT gene (5-HTTVNTR), 5-HT2A receptor gene (5-HT2A G-1438A), a TPH gene polymorphism in intron 7 (TPH A218C), norepinephrine transporter (NET) gene polymorphism in the promoter region (NET T-182C) and in the exon 9 (NET G1287A), a variable number of tandem repeats in the promoter region of monoamine oxidase A, were items to be assessed in this study. Ninety-six patients with major depressive disorder were treated with milnacipran. Side effects were assessed at 1, 2, 4, and 6 weeks of treatment with Udvalg for Kliniske Undersogelser side effects scale. The results showed that no gene polymorphisms included in this study affected the susceptibility of nausea and excessive sweating induced by milnacipran. Patients with older age are more likely to develop excessive sweating than others. The major limitation of this study is a small sample size. Further studies with larger populations and more kinds of gene polymorphisms should be needed to see if specific gene polymorphisms determine the susceptibility of side effects induced by milnacipran. Keywords: milnacipran, nausea, excessive sweating, gene polymorphisms
Lipoprotein Lipase as a Candidate Target for Cancer Prevention/Therapy
Shinji Takasu,Michihiro Mutoh,Mami Takahashi,Hitoshi Nakagama
Biochemistry Research International , 2012, DOI: 10.1155/2012/398697
Abstract: Epidemiological studies have shown that serum triglyceride (TG) levels are linked with risk of development of cancer, including colorectal and pancreatic cancers, and their precancerous lesions. Thus, it is assumed that serum TG plays an important role in carcinogenesis, and the key enzyme lipoprotein lipase (LPL), which catalyzes the hydrolysis of plasma TG, may therefore be involved. Dysregulation of LPL has been reported to contribute to many human diseases, such as atherosclerosis, chylomicronaemia, obesity, and type 2 diabetes. Recently, it has been reported that LPL gene deficiency, such as due to chromosome 8p22 loss, LPL gene polymorphism, and epigenetic changes in its promoter region gene, increases cancer risk, especially in the prostate. In animal experiments, high serum TG levels seem to promote sporadic/carcinogen-induced genesis of colorectal and pancreatic cancers. Interestingly, tumor suppressive effects of LPL inducers, such as PPAR ligands, NO-1886, and indomethacin, have been demonstrated in animal models. Moreover, recent evidence that LPL plays important roles in inflammation and obesity implies that it is an appropriate general target for chemopreventive and chemotherapeutic agents. 1. Introduction A high-calorie diet and low physical activity, part of the so-called “Westernization” of lifestyle, are associated with elevated incidences of the breast, colon, liver, pancreas, and prostate cancers. Moreover, they are also linked with the risk of obesity, type 2 diabetes, and dyslipidemia. The World Cancer Research Fund and American Institute for Cancer Research have evaluated causal relationships between body fat and cancer and provided strong evidence for roles in such as colorectum and pancreas cancers [1]. In Japan, overweight and obesity (body mass index ≥25) are reported to be associated with cancers of specific organs, such as the colorectum (male), postmenopausal breast (female), and the liver in individuals positive for hepatitis C virus infection [2–4]. Greater body fatness is a major risk factor for the metabolic syndrome, which presents as a combination of symptoms, such as dyslipidemia (elevated triglyceride (TG) levels or low high-density lipoprotein (HDL) cholesterol), elevated blood pressure, and elevated fasting glucose levels. Hypertriglyceridemia is associated with the risk of colon cancer in Japanese men ( ) and being overweight with the risk of breast cancer ( ) [5]. In addition, most epidemiological studies, including our own, have consistently showed that serum TG levels are associated with the risk of colorectal
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