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Search Results: 1 - 10 of 310046 matches for " <br>黄姗 "
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Determination of Salicin in Extract of Willow Bark by High Performance Liquid Chromatography

LI Lai sheng,HUANG Wei dong,HE Qi,YE Shan,<br>李来生,伟东,何琦,
色谱 , 2001,
Abstract: An HPLC method for the determination of salicin in extract of willow bark is described. Chromatographic analysis was carried out on a Kromasil C18, 5 microns column(4.6 mm i.d. x 250 mm) with methanol-0.01 mol/L KH2PO4 buffer (pH 4.01) (15:85, volume ratio) as mobile phase. The detection wavelength was 265 nm. Salicin was extracted from samples with methanol-water(50:50, volume ratio), and centrifuged. Ten microL of supernatant were injected. The average recoveries were from 96.1% to 101.2% (n = 5), and the relative standard deviation (RSD) was 1.43%. The method is simple, rapid and accurate.
Synthesis and Characterization of Micro- and Nano- Metal-porphyrin Coordination Polymer

- , 2016,
Abstract: 微纳米尺度的金属有机配合物不仅具有纳米尺寸效应,而且具有可调的组装单元,从而表现出诸多良好性能。我们通过一种简单的绿色合成方法,得到微纳米尺度的金属卟啉配合物,并通过选择不同的金属离子,实现了对金属卟啉配合物的可控组装,得到了从零位、一维和二维的金属卟啉配合物。并借助扫描电镜、红外光谱、X射线衍射谱(XRD), X射线光电子能谱(XPS)等对其结构进行了表征,详细考察了其影响纳米结构的影响因素和动力学生长过程。
Micro- and nano-scale metal-organic complexs not only have size effect, but have an adjustable assembly unit, which shows a lot of good performance. in this paper, we synthesized nanoscale mertal-porphyrin complexs by a simple and green method. by choosing the different metal ions, the different nanostructures (0D, 1D, 2D) of metal-porphyrin polymer was abtained by controlled assemble unit. its structures were characterizatized by scanning electron microscopy (SEM), infrared spectroscopy (IR), x-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), and were detailed examinated of the impact and dynamica growth of the metal-porphyrin polymer
Cloning and expression analysis of an hydrophobin gene Nrhyd in Nomuraea rileyi

HUANG Shan,WANG Zhong-Kang,CHEN Huan,WANG Meng,YIN You-Ping,<br>,王中康,陈环,王萌,殷幼平
菌物学报 , 2012,
Abstract: The full-length cDNA of a hydrophobin gene Nrhyd gene was cloned from the insect pathogenic fungus Nomuraea rileyi using SMART RACE RT-PCR. The expression of Nrhyd gene by N. rileyi under different culture conditions was analyzed using Realtime-PCR, and the effects of different culture conditions on expression of Nrhyd gene on mRNA level were explored. Sequence analysis shows that the cDNA contains an open reading frame of 339bp which encoding a polypeptide with 111 amino acids. The precursor protein has a molecular mass of 10.6 and a calculated pI of 6.19. The RT-qPCR results showed that the expression of Nrhyd gene was suppressed under liquid culture condition. While under solid agar culture condition, the expression level of Nrhyd gene increased along with the formation of conidia and reached the highest level at day 8 of post-inoculation, and then decreased gradually. It is inferred therefore that the Nrhyd gene might play an important role in the formation of conidia in N. rileyi. Phylogenetics analysis of Nrhyd gene with other fungal hydrophobin indicates the gene is most closely related to the homologues from Metarhizium spp.
Role of human TIMP-2 protein in the migration and invasion of hepatocellular carcinoma cell

王伟,曹煜,孙达权,小琼,徐国强<br>WANG Wei, CAO Yushan, SUN Daquan, HUANG Xiaoqiong, XU Guoqiang
- , 2016, DOI: 10.6040/j.issn.1671-7554.0.2016.132
Establishment of integrated methodology for bay ecosystem health assessment and its application in Daya Bay

LI Chunhou,LIN Lin,XU Shannan,DAI Ming,HUANG Honghui,DU Feiyan,LIU Yong,QI Zhanhui,<br>李纯厚,林琳,,戴明,洪辉,杜飞雁,刘永,齐占会
生态学报 , 2013,
Abstract: Bay ecosystem health, recently, has faced a growing interference and disturbance by anthropogenic activities, which has also caused challenges to ecosystem management. Ecosystem health assessment, a new methodology of ecosystem assessment, could obtain the current situation both of ecosystem structure and function and provide a great of necessary information for ecosystem management. An integrated assessment methodology for bay ecosystem health was established based on geographical information system (GIS) technology, which was on basis of a "pressure-structure-response" frame model for marine ecosystem and an ecosystem health assessment indicator system constructed by external pressure, ecosystem structure and response parameters. This paper also presented a case study of Daya bay in spring by employing GIS technology.The results showed that the average ecosystem health integrated index was 0.57 in Daya Bay ecosystem in spring. Detailedly, organic pollutant index ranged from 0.36 to 1, with the average value at 0.87, was at "very good" level of Daya Bay ecosystem. Eutrophic level index value ranged from 0.17 to 1, with the average of 0.77, indicated "better" level of the ecosystem. Furthermore, phytoplankton abundance index was at a "worse" level because of the ranges from 0.33 to 1 and the average of 0.21. Zooplankton biomass index ranged from 0.16 to 1, with the average of 0.88, suggested the ecosystem was at a "very good" level. Zoobenthos biomass index was at "critical state", as the index value ranged from 0 to 1 and the average value at 0.59. Moreover, phytoplankton diversity index was also "critical state" level, as the index ranged from 0.39 to 0.75 and the average of 0.55. Primary productivity index, ranged from 0.21 to 1 and the average of 0.77, illustrated the "better" level of the ecosystem. However, ecological buffer capacity index was at a "worst" level, as the index value ranged from 0 to 0.39 and the average value of 0.06. Resumptively, spatial distribution of the assessed sea area could be divided into three zones according to the grade of integrated assessment index, namely "good health zone", "critical health zone" and "bad health zone". The percentages of three zones were 46.56%, 47.97% and 5.47%, with the average integrated health index of 0.64, 0.54 and 0.35, respectively.In general, the status of the Daya Bay ecosystem health was at a "better" level according to the above indexes. However, the health status of the ecosystem was potential to change to "critical state" by the negative influences by factors including phytoplankton abundance, phytoplankton diversity and buffer capacities of the ecosystem. The "bad health zone" in the Daya Bay ecosystem distributed at or near the area with the intensive anthropogenic activity. Consequently, the special distribution of Daya Bay ecosystem health status was mostly affected by anthropogenic activity around the bay. The established methodology has been ultimately proved to be a valu
On the divergence problem of temperature fluctuations in simple systems

Hou Ji-Xuan,Wang Xin,Huang Shan,Lin Jian-Jun,Wan Cheng-Lan,Liu Quan-Hui,<br>侯吉旋,王 鑫, ,林建军,万承兰,刘全慧
物理学报 , 2006,
Abstract: The presupposition of deriving the fluctuation from equilibrium thermodynamics is that the fluctuation must be small, yet for some systems the derived temperature fluctuations are divergent as the temperature approaches 0K. For such fluctuations, the statistical physics has to be utilized to yield the correct results. Our results show that the fluctuations go to zero as the temperature approaches 0K.
Effect of Pseudomonas aeruginosa on the production of lignocellulase from Phanerochaete chrysosporium

WANG Pingping,YUAN Xingzhong,LIANG Yunshan,ZENG Guangming,HUANG Huajun,ZHU Huin,CHAO Yang,SUN Ting,<br>汪萍萍,袁兴中,梁运,曾光明,华军,祝慧娜,晁阳,孙婷
环境科学学报 , 2011,
Abstract: The effects of Pseudomonas aeruginosa on the production of lignin peroxidase, manganese peroxidase and CMCase from Phanerochaete chrysosporium in a solid substrate fermentation process were studied. Effects of different concentrations of Ps. aeruginosa on other parameters, such as pH, surface tension of crude enzyme liquid, volatile organic matter concentration were also evaluated. The results showed that Ps.aeruginosa AB93066 at 5.72% increased the production of LiP, MnP and CMCase by 67.8%, 57.9% and 17.8%, respectively while strain Ps. aeruginosa ATCC9027 at 2.86% increased the production of LiP, MnP and CMCase by 86.3%, 49.3% and 43.7%, respectively. The surface tension of crude enzyme liquid from P. aeruginosa ATCC9027 was lower than that from Ps. aeruginosa AB93066. The addition of Ps. aeruginosa had no significant influence on the pH value. The volatile organic matter concentration was positively correlated with the enzymatic activity.
Assessment model for heavy metal pollution in river sediment based on triangular fuzzy numbers

FAN Mengji,YUAN Xingzhong,ZHU Huin,HUANG Huajun,ZENG Guangming,LIANG Yunshan,JIANG Hongwei,<br>樊梦佳,袁兴中,祝慧娜,华军,曾光明,梁运,江洪炜
环境科学学报 , 2010,
Abstract: 将模糊集理论引入污染评价领域,建立基于三角模糊数的地累积指数模糊评价模型.用三角模糊数表示沉积物污染物浓度和地球化学背景值,并通过α-截集技术和区间数的隶属度计算得出重金属污染程度级别,从而为重金属的综合污染评价提出一种新的思路.将该模型应用于湘江7个河段底泥沉积物重金属污染的评价,结果表明,7个河段沉积物中重金属污染严重,且6种重金属的地累积指数差异较大,各种重金属的富集程度由高至低排列的顺序为:Cd>Zn>Hg>Pb>As >Cr.各河段沉积物中重金属的综合污染程度顺序为:衡阳>湘潭>郴州>长沙>岳阳>株洲>永州.Cd、Zn和Hg是各河段沉积物污染的主要环境污染因子.
中国高速增长的“半城市化”调节模式:布瓦耶和阿瑞吉的比较与补充 The Regulation Mode of“PeriUrbanization” in Chinas HighGrowth Economy: A Comparative and Complementary Analysis of R. Boyer and G. Arrighi
- , 2017,
Abstract: 摘要 法国调节学派代表人物布瓦耶近期对“中国体制”的概括值得关注。他将城乡分割的“半城市化”调节模式视为支撑中国经济高速增长的制度基础,并基于发达工业化国家的经验对这一体制提出批评。这与肯定中国“半无产阶级化”现象的社会历史学派代表人物阿瑞吉的观点形成了对比。就法国调节学派概括“中国体制”的整体性分析框架与社会历史学派分析中国特色发展道路的历史性、民族性视角进行比较与综合,有助于我们准确把握中国高速增长的制度条件和历史背景。鉴于布瓦耶和阿瑞吉所掌握的统计数据和制度细节尚不全面,对“半城市化”历史制度根源的认识存在一定局限性,我们尝试基于具体的统计数据和历史制度背景对他们的观点进行补充,以期在发展的视角下全面准确地认识中国高速增长的“半城市化”调节模式。
大豆科学 , 2012, DOI: 10.3969/j.issn.1000-9841.2012.04.008
Abstract: 在对大豆根系盐胁迫抑制差减杂交文库EST序列分析的基础上,利用RT-PCR技术克隆得到了一个大豆?UspA?基因,命名为GmUsp1,其编码一个包含164个氨基酸残基的多肽链。多序列比对和编码蛋白结构分析表明,GmUsp1属于泛应激蛋白(UniversalStressProtein)家族成员之一。其氨基酸序列与蚕豆Vf_enod18序列相似度最高,属于MJ-0577类中含有ATP结合位点的UspA亚族,与MJ-0577有相似的蛋白二级结构。分别采用250mmol?L-1NaCl、100μmol?L-1ABA和30%PEG6000进行胁迫处理,耐盐品种文丰7号和盐敏感品种Union的GmUsp1均被诱导表达,二者对胁迫诱导响应时间和表达量存在差异,说明GmUsp1可能参与大豆对非生物逆境胁迫的应答调控。
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