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Search Results: 1 - 10 of 292926 matches for " <br>赫荣乔 "
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FLUOROPHORE FORMATION OF YEAST D GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE IN POTASSIUM IODIDE SOLUTION
酵母GAPDH在碘化钾溶液中荧光发色团的形成

He Rongqiao,<br>
生物物理学报 , 1992,
Abstract: 酵母GAPDH的剩余活力在碘化钾为0.1M溶液中趋近于零,内源荧光在碘化钾为0.1—0.4M时迅速被淬灭,在0.5—2.0M时基本不变,3.0M时达到最低。并在一定浓度的碘化钾溶液中形成新的荧光发色性质(Ex336nm;Em383nm),量子产率大约为0.2,但形成该荧光发色团较为缓慢。与NAD荧光衍生物(Chen-LuTsou,etal.(1983)Biochem.Soc.Trans.11,425—429)不同的是,GAPDH在碘化钾溶液中形成荧光发色团时不需要光的激发。肌酸激酶及胰岛素不形成这种荧光衍生物。
COMPARISON OF THE DENATURATION BY GUANIDINIUM CHLORIDE BETWEEN ALL OF-THE SITES AND HALE OF-THE-SITES MODIFIED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
3-磷酸甘油醛脱氢酶的胍变性——全位与半位修饰酶的比较

He Rongqiao,<br>
生物物理学报 , 1991,
Abstract: Under carefully controlled conditions, yeast GAPDH carboxymethylated at only 2 or all 4 of the subunits can be respectively produced fluorescent NAD derivative at 1 or 2 of the decarboxylated subunits by irradiation in presence of NAD+ (Tsou, et al, Biochem. Soc. Trans. 11, 425-429, 1983). The emission peak at 410nm of the fluorescent NAD derivative introduced at the active site of this enzyme shows both a red shift and a marked decrease in intensity at the same GuHCl concentration required to bring about the inactivation and the initial changes in the intrinsic fluorescence of the holoenzyme. The conformation of modified GAPDH carrying the fluorescent NAD derivative at one subunit is relatively less perturbed than that carrying the derivative at two subunits. The kinetics of this emission change is also biphasic with a fast phase approaching that for the fast phase of inactivation. It appears that treatment by low concentration GuHCl leads both inactivation and perturbation of the active site conformation and these are then followed by unfolding of the molecule as a whole.
FORMATION AND PROPERTIES OF OXOACYL FLUORESCENT DERIVATIVE ON N-TERMINALS OF PROTEINS AND PEPTIDES
肽链及蛋白质N-末端羰酰荧光衍生物的形成

He Rongqiao,Tsou Chenlu,<br>,邹承鲁
生物物理学报 , 1991,
Abstract: Dixon transamination of oligopeptides and insulin results in the formation of a fluorescent derivative having emission maximum about 398-408nm with 0.02-0.03 quantum yield by the excitation at 3l2-333nm depending on the nature and length of the peptide. The chemical structure of the fluorophore may be ?-Oxo-acyl-amide which would be effected by thd second and the third amino acid residues, its intensity decreases when pH>9.0 and increases with the increment of concentrations of NaCl solution. Changes of the Oxoacyl-amide fluorescence of oligopeptides are comparatively different with their different kinds or configurations of the composed residues. It is suggested that such a fluorescence derivative may be used as a probe for the study of the conformation of the N-terminal region of peptides and proteins.
COMPARISON OF INACTIVATION AND CONFORMATIONAL CHANGES OF YEAST D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE DURING DENATURATION BY GUANIDINIUM CHLORIDE
3-磷酸甘油醛脱氢酶胍变性时的活力及构象变化

He Rongqiao,Tsou Chenlu,<br>,邹承鲁
生物物理学报 , 1991,
Abstract: Changes in intrinsic protein fluorescence of yeast GAPDH have been compared with its inactivation during denaturation in GuHCI solutions. The holo-and the apo-enzymes are completely inactivated at GuHCI concentrations less than 0.5M and this is accompanied by marked decrease in flurescence emission intensity and red Shift of the emission maxima at 335 nm suggesting exposure of aromatic residues. It has been reported before that during the denaturation of the muscle enzyme, following an initial decrease, there is a region of relatvely little fluorescence changes between 0.4-1.2 M GuHCI.For the yeast enzyme, the decrease in emission intensity is continuous with the increase in GuHCI concentration. At 0.7M GuHCI, the fluorescence change is monophasec with a first order rate constant of 1.3×10-4S-1 but the inactivation is a biphasic process with a fast phase rate constant greater than 0.1×S-1. This is 3 order of magnitudes faster than the corresponding unfolding rate. The slow phase inactivation rate, first order constant of 2.1×10-4 S-1 , is close to that of the unfolding rate as measured by fluorescence changes.
Endophytic Streptomyces Strains of Rice Plant Containing Circular as well as Linear Plasmids
水稻内生链霉菌中的线型和环型质粒

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: 植物内生放线菌的研究是一个近年来兴起的学科领域,在进一步探索和开发微生物资源方面,植物内生放线菌逐渐成为相关领域同行的关注热点.
A Novel Endophytic Bacteria H-6 with High Antifungal Activity from Huperzia serrata
一株新的药用植物内生菌Burkholderia sp. H-6

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: 植物内生菌(Plant endophyte)是微生物中的一个重要类群,能参与植物次级代谢产物的合成与转化或独立合成次级代谢产物,其物种丰富,数量庞大,已经成为新医(农)药活性物质的潜在资源.研究显示,目前从植物内生菌中分离出的生物活性物质,大约51%属于未报道过的新化合物.因此,植物内生菌相关领域的研究工作,愈来愈受到国内外同行的关注.
One of Sulfate-reducing Bacteria in the Corrosion Biofilm on Metal Pipeline
硫酸盐还原菌与油田金属管道腐蚀

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: 硫酸盐还原菌(sulfate-reducing bacteria,SRB)是一类利用硫酸盐和其他氧化态硫化物,或利用元素硫作为电子受体,并将其还原成S2-的原核生物。在长时间开采原油过程中,地层需要通过金属管道注水来维持油气输出压力,而输水管壁上很容易形成生物膜垢。该生物膜垢的形成是微生物腐蚀发生的必要条件,也是微生物对金属腐蚀问题难以解决的主要原因。
On the Technique for L-cystine Conversion by Pseudomonas putida TS1138
微生物酶法转化生产L-半胱氨酸的工艺研究

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: L-半胱氨酸(Cys)侧链巯基是构成蛋白质活性基团的重要氨基酸,在生物化学、医药、食品、饲料、化妆品等行业具有广泛的用途,国内外的需求量逐年增长.然而,Cys难以通过单纯的微生物发酵来进行生产;由于化学合成的步骤繁多,也很难进行化学合成.传统生产方法沿用毛发酸解制取L-半胱氨酸,收率低,能耗高,水解过程产生难闻气体及大量废酸,环境污染严重.
A Harpin Protein from Erwinia carotovora subsp. Betavasculorum (Ecb) Strain
胡萝卜软腐欧文氏菌甜菜亚种Ecb菌株表达的一种Harpin蛋白

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: 1986年,hrp基因被首次报道.由于Wei等(1992)从梨火疫病菌(Brwinia amylovora)中分离出了能激发过敏反应的HarpinEa蛋白,细菌所产生的Harpin分子受到了同行的重视.基于对Harpin蛋白的深入研究,国外已研制出了相关新型、高效、安全的生物农药.近年来,国内涉及Harpin蛋白结构与功能的研究课题组
Isolation of Polyketide Synthase I Cluster from the Environmental Strain X-2 Producing Macrolactins
从海洋细菌X-2中筛选Ⅰ型PKS基因簇

HE Rong-Qiao,<br>
微生物学通报 , 2008,
Abstract: 与发达国家的相关工作相比较,我国对海洋微生物功能基因资源的利用和开发尚处于初期.因此,在国内开展海洋微生物功能基因的研究和利用,不但在相关领域具有理论意义,同时具有潜在的社会效益和应用价值.Macrolactins是一类24员大环内酯类化合物,具有抗菌、抗病毒和抗肿瘤等多种生物学活性.国际同行的研究表明,Macrolactins有18个家族成员(Macrolactin A-R),主要为海洋来源的Actinomadura sp.和Bacillus sp..
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