oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 146 )

2018 ( 6898 )

2017 ( 6860 )

2016 ( 7045 )

Custom range...

Search Results: 1 - 10 of 374127 matches for " <br>罗庆华 "
All listed articles are free for downloading (OA Articles)
Page 1 /374127
Display every page Item
Habitat characteristics of Andrias davidianus in Zhangjiajie of China
张家界大鲵生境特征

LUO Qing-hua,<br>
应用生态学报 , 2009,
Abstract: By the methods of field investigation and laboratory analysis, the habitat characteristics of Chinese giant salamander (Andrias davidianus) in Zhangjiajie Giant Saramander National Nature Reserve were studied from July to August in 2006-2008. In the study area, the inhabited reaches of A. davidianus were characterized by moderate altitude (373±97 m), relatively small river width (6.39±2.13 m) and depth (1.07±0.53 m), lithoid substrate with more gravels or rocks, irregular river bed, stony river bank with more scrub vegetation and larger coverage (>50%), steeper bank gradient (58°±17°), higher water flow rate (0.33±0.08 m·s-1), and weaker anthropogenic disturbances. There were many burrows (4±2 ind·100 m-1) in the habitat, and the water flow rate in the burrows were relatively low (0.22±0.10 m·s-1). The waters in the habitat were with high DO (7.23±0.85 mg·L-1), low COD (4.00±2.51 mg·L-1), low TN (0.11±0.15 mg·L-1), moderate total hardness (137.16±67.69 mg·L-1), and lesser coliform groups (3419±3407 ind·L-1). Crabs were the most important food organisms affecting the A. davidianus population in the study area, followed by shrimps.
Effectiveness of releasing artificially-bred Chinese giant salamander (Andrias davidianus) into the wild in Zhangjiajie, Hunan
张家界大鲵人工放流效果及其影响因素分析

Qinghua Luo,Ying Liu,Liyun Zhang,<br>,刘英,张立云
生物多样性 , 2009,
Abstract: 大鲵(Andrias davidianus)是我国特有的两栖动物, 由于栖息地破坏和人类过度捕杀等, 种群数量急剧下降, 被列为我国II类保护野生动物。为探索大鲵人工放流的有效方法, 实现野生大鲵资源增殖, 作者对2002–2008年间在湖南张家界大鲵国家级自然保护区内进行的大鲵人工放流活动与放流效果进行了评估, 并通过对放流点的隐蔽物、水质及饵料生物等生境特点的分析, 探讨影响放流成败的因素。该保护区内共在9处大鲵原栖息地进行了11次人工放流活动, 共放流各种规格大鲵995尾, 其中4次成功。成功放流的大鲵为1–4.5 kg的性成熟个体, 雌雄比在1:1和1:1.5之间, 成功放流点均有良好的后期管理措施, 如建立保护站, 补充饵料等。虽然所选择的多数放流点生境基本能满足大鲵生长和繁殖的要求, 但由于受水质、饵料或安全某一因素的制约, 野生大鲵资源增殖效果不明显, 导致放流失败。因此我们认为依据野生大鲵繁殖的生境特征标准, 科学选择放流地点, 选择性成熟的大鲵在春季放流, 并进行完善的后期跟踪保护, 可能是大鲵人工放流成功和实现资源增殖的有效途径。
张家界大鲵生境特征

应用生态学报 , 2009,
Abstract: 2006—2008年的7—8月,对湖南张家界大鲵国家级自然保护区境内大鲵栖息河段及其洞穴特征、水质和饵料生物资源进行了实地观测和取样分析,研究了该保护区野生大鲵的生境特征.结果表明:研究区大鲵栖息河段海拔适中,在(373±97)m,河宽与河深均较小,分别为(6.39±2.13)m和(1.07±0.53)m;河段底质均为石质性结构,多为卵石或礁石,河床多为不规则型,河岸多为石壁;两岸植被类型多为灌木丛,植被覆盖度(>50%)与坡度[(58±17)°]均较大;研究河段的水流速较大,在(0.33±0.08)m·s-1,且人为干扰较弱.研究区大鲵栖息地的洞穴数量较多,达(4±2)个·100m-1,洞穴中水的流速较小,在(0.22±0.10)m·s-1.大鲵栖息处水质溶解氧较高,为(7.23±0.85)mg·l-1,化学耗氧量与总氮含量均较低,分别为(4.00±2.51)和(0.11±0.15)mg·l-1,总硬度适中,为(137.16±67.69)mg·l-1,大肠菌群较少,为(3419±3407)ind·l-1.对研究区大鲵种群数量影响最大的饵料生物是蟹,其次为虾.
成都市青龙场鸟类栖息地植物的选择和配置
,辜 彬
北方园艺 , 2010, DOI: 10.11937/bfyy.201021048
Abstract:
Effects of allelopathic rice on rhizosphere microbial flora and enzyme activity
化感水稻根际微生物类群及酶活性变化

HU Kaihui,LUO Qingguo,WANG Shihua,LIN Xuan,LIN Wenxiong,<br>胡开辉,,汪世,林旋,林文雄
应用生态学报 , 2006,
Abstract: This paper studied the dynamics of microbial flora and enzyme activity in the rhizosphere of allelopathic rice PI312777 (PI) and non-allelopathic rice Lemont (LE) at the growth stage of 3 - 7 leaves. The results showed that in the rhizosphere of PI, the amounts of bacteria, actinomycetes and azotobacter were 11.2% - 28.3%, 40% - 78.6% and 111.5% - 173.9%, respectively, while that of fungi was lower, with the maximum being 25.5% of that in the rhizosphere of LE, suggesting that allelopathic rice PI promoted the growth of bacteria, actinomycetes and azotobacter, but inhibited that of fungi. Further analysis on the physiological groups of microbial flora showed that PI favored the growth of ammonifier, aerobic azotobacter, aerobic cellulose - decomposer, sulphate - reducer, nitrite - bacteria and nitrate - bacteria, among which, ammonifier and aerobic azotobacter increased by 53.7% and 57.6%, respectively, while inhibited the growth of desulphate bacteria and denitrifyier. Moreover, PI increased the activities of urease, phosphatase and sucrase, but decreased the catalase activity in its rhizosphere.
A Semliki Forest virus RNA replicon-based DNA plasmid encoding classical swine fever virus E2 gene induces immune response in mice
基于Semliki Forest病毒RNA复制子的猪瘟RNA疫苗初步研究

Hua-Ji Qiu,<br>李娜,,李国新,,玉子,贾洪林,童光志
中国生物工程杂志 , 2005,
Abstract: 将猪瘟病毒E2基因克隆于我们此前构建的衍生于Semliki Forest病毒(semliki forest virus,SFV)RNA复制子的新型真核表达载体pSFV1CS中,获得重组质粒pSFV1CS-E2。用纯化的pSFV1CS-E2分别转染BHK-21细胞和293T细胞,经间接免疫荧光试验检测显示,CSFV E2基因在转染细胞中得到表达。小鼠接种试验结果表明,10μg或100μg pSFV1CS-E2可诱导小鼠产生猪瘟特异性抗体。
A Modified Algorithm of Subgraph Isomorphism Based on Decomposition
改进的基于分解的子图同构算法

ZHANG Zhi-Xiang,LI Qing-Hua,LUO Jian-Ming,<br>张志祥,,建明
计算机科学 , 2006,
Abstract: t The algorithm of subgraph isomorphism based on decomposition proposed by Messmer et. is analysed, and its problems are pointed out. A revised algorithm is given, which adapted the decomposition and combination procedure of the original algorithm, and resolve the problems of the original algorithm with higher effeeienee. The experimental result shows that this algorithm is correct.
《拉丁美洲标准刑法典》(1971年)
,树中
中外法学 , 1992,
Abstract:
VEGF特异siRNA重组腺病毒构建及其对K562细胞增殖的抑制作用
李方方,徐酉,
第三军医大学学报 , 2009,
Abstract: 目的构建重组腺病毒Ad5-VEGFsi观察其抑制人红白血病K562细胞血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)表达及其对K562细胞增殖抑制作用的影响。方法①合成针对人VEGFmRNA的特异性siRNA;将其插入穿梭质粒pSES-HUS,命名为pSES-VEGFsi;后者与腺病毒骨架质粒pAdEasy-l同源重组。酶切鉴定成功后,于HEK293细胞中包装、扩增,氯化铯超速梯度离心后得到滴度较高的重组腺病毒Ad5-VEGFsi;②Ad5-VEGFsi以最适感染复数(multiplicityofinfection,MOI)感染K562细胞72h后,RT-PCR及ELISA方法检测VEGFmRNA及细胞培养液上清VEGF蛋白表达、MTT法检测细胞增殖、流式细胞仪测定细胞凋亡率。结果成功构建重组腺病毒Ad5-VEGFsi,氯化铯梯度离心纯化获得重组腺病毒滴度约4.6×1011pfu/ml;Ad5-VEGFsi感染K562细胞72h后,VEGFmRNA水平与对照组相比下降了66.55%(P<0.01),蛋白浓度下降了86.03%(P<0.01)。感染Ad5-VEGFsi后K562细胞生长明显受到抑制,且细胞的凋亡率也较对照组增加了13.89%(P<0.01)。结论重组腺病毒腺Ad5-VEGFsi可明显抑制人白血病K562细胞VEGF的表达,从而抑制细胞增殖,诱导细胞凋亡。
张家界大鲵人工放流效果及其影响因素分析
,刘英,张立云
生物多样性 , 2009,
Abstract: ?大鲵(andriasdavidianus)是我国特有的两栖动物,由于栖息地破坏和人类过度捕杀等,种群数量急剧下降,被列为我国ii类保护野生动物。为探索大鲵人工放流的有效方法,实现野生大鲵资源增殖,作者对2002–2008年间在湖南张家界大鲵国家级自然保护区内进行的大鲵人工放流活动与放流效果进行了评估,并通过对放流点的隐蔽物、水质及饵料生物等生境特点的分析,探讨影响放流成败的因素。该保护区内共在9处大鲵原栖息地进行了11次人工放流活动,共放流各种规格大鲵995尾,其中4次成功。成功放流的大鲵为1–4.5kg的性成熟个体,雌雄比在1:1和1:1.5之间,成功放流点均有良好的后期管理措施,如建立保护站,补充饵料等。虽然所选择的多数放流点生境基本能满足大鲵生长和繁殖的要求,但由于受水质、饵料或安全某一因素的制约,野生大鲵资源增殖效果不明显,导致放流失败。因此我们认为依据野生大鲵繁殖的生境特征标准,科学选择放流地点,选择性成熟的大鲵在春季放流,并进行完善的后期跟踪保护,可能是大鲵人工放流成功和实现资源增殖的有效途径。
Page 1 /374127
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.